Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947648,2500AC51.5% ‑2.4 / 18.3 34intergenic (+16/+75)acoR/accCacetoin catabolism regulatory protein/acetyl‑CoA carboxylase biotin carboxylase subunit
Reads supporting (aligned to +/- strand):  ref base A (0/16);  new base C (0/17);  total (0/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGCTGATCCGCCATCGCTGGAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTC‑GCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGG‑CCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGC  >  NC_002947/648116‑648380
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ggtttaTCCGCCATCGGTGTACGCGGGCTTTGGGGGCAGGGGCTTTGGGATTTTC‑GCGGGCGACGATTTATCGGCGTGTGAATGACCCCCGCATCGACATGCCGCGTTTTACGGGGTAGCGCCGTGGGG‑CCGCGAc                                                                                                                                   <  2:3187191/131‑1 (MQ=255)
    tagccGCCATGGCTGGACGCGGGCTTTGGGGGCCGGGGCTTTGGGAATTCC‑GCGGGCGACGCTTTATCGGCGAGTGCATCACCACCGCATCCACATGCCGCGTATGACGGGGTAGCCCCGGGGGG‑ccgcgccgcgc                                                                                                                               <  2:11734/133‑2 (MQ=255)
                                    ccggggCTTTGGGCATTCC‑GCGGGCGACGCTTTAGCGGCGAGTGCATGCCCACCGCAGCGACAGGCCGCGGATGATGGGGTAGCGCCCGGGGG‑CCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCg                                                                                               <  2:2403203/133‑1 (MQ=255)
                                    ccggggCTTGGCGCTTTCG‑GCGGGCCCCGCTTTCGCGGGGCGGGCTTGCGCCCCGCACCGACTGGCCGCGGTTGAGGGGGTCGCCCCGGGGGG‑CCGCGCGGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCg                                                                                               <  2:3251229/133‑1 (MQ=255)
                                    caggggCTTTGGG‑ATTTCGGGGGGCTACGTTTTATGGGCCTGTCCTTGAGCACCGCATCGACATGCGGCGTATTATGGGTTAGCGCCGGGGGG‑CCGCGCCGCGCCCCCAGATTCTGGCAGGCAATAAAAAACCCCg                                                                                               <  2:3321388/133‑1 (MQ=255)
                                    cACGGTGTTGGGGAATTTC‑GCGGCCGACGATTTATCGGCGTGGGCATGACCACCGAATCTAAAGGCCGCGGATGAAGGGTTCGCGCCCTGGGG‑CGGCTACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCg                                                                                               <  2:2698460/136‑1 (MQ=255)
                                    cACGGGGTTTGGCATTTTC‑GCGGGCGCCGCTTTATCGGCGTGTGCATCAGCACCGAATCGACATGCCGCGTATTAAGGGGTAGCGCCCGGGGG‑CCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCg                                                                                               <  2:1115060/136‑1 (MQ=255)
                                    cACGGGGTTGGGGCTTTTC‑GCGGGGGACGCTTTATGGGCGTTGGCTTGAGCACCGCTTCGACTTGCGGGGGTTGAAGGGTCACCGCCCTGGGG‑CCGGTCCGGGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCg                                                                                               <  2:2673421/136‑1 (MQ=255)
                                    cACGGGCTTTGGGGATTTC‑GCGGGCGACGCTTTATCGGCGAGTGCATTAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCGGGGG‑CCTCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCg                                                                                               <  1:3976423/136‑1 (MQ=255)
                                    cACGGGCTTTGGCCTTTGC‑GGGGGCGACGCTTTATCGGCGTGTCCATCCCCCCCGCATCGACTGGCCGCGGATGACGGGGTCGCCCCCGGGGG‑CCGCGCCGCGCCCCCAGATTCTGGCAGGCAATAAAAAACCCCg                                                                                               <  2:2205947/136‑1 (MQ=255)
                                            ttGGCCTTTGC‑GCGGGCCCCGCTTTTGCGGCGAGGGCATGACCCCCGCATCCACATGCCGCGGATGACGGGGTAGCGCCCGGGGG‑CCGCGCCGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAggg                                                                                       <  2:3638234/136‑1 (MQ=255)
                                               ggCTTTGC‑GGGGGCTCCGCTTTAGGGGCGCGTCCATCCCCACCGCACCGACCGGCGGGGTTTGAGGGGGTAGCCCCGGGGGG‑CCGCGCCGGGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCg                                                                                    <  2:4577263/136‑1 (MQ=255)
                                                        gcgGCCGACTCTTTCTCGGCGTGTCCATTCCCCCCGAATCTACA‑GCCCCGGTTGGAGGGGTACCCCCCCGGGGCCCGCTCCGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTg                                                                            <  2:883639/136‑1 (MQ=255)
                                                             cgccGCTTTATCGGCGCGTGCATCCCCCCCGCTCCGCCAGGCCGCGTTTGAGGGGGTCGCGCCGGGGGG‑CCGCGCCGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACa                                                                       <  2:16062/133‑1 (MQ=255)
                                                             cccggCTTTAGCGGCGAGTGCATGACCCCCCCATCGACAGGCCGCGGATGACGGGGTCGCGCCCGGGGG‑CCGCGACGCGCCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACa                                                                       <  2:3607912/132‑1 (MQ=255)
                                                             cGACGTTTTAGCGGCGTGTCCATGCGCACCGCATCTACTTCCCGCGTTGGACGGGGTAGCGCCGGGGGG‑CCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACa                                                                       <  2:401369/136‑1 (MQ=255)
                                                             cGACGCTTTAACGCCGAGGGCACGACCACCGCATCCACATGCCGCGCATGAAGGGCCCCCCCCCTGGGG‑CCGCGCCGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACa                                                                       <  2:3114758/136‑1 (MQ=255)
                                                               ccGCTTTAGCGGCGAGGCCAGCCCCACCGCACCCCCACGCCGCGTATGACGGGGCCGCCCCCGGGGG‑CCGCGCCGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATc                                                                     <  2:3650823/135‑1 (MQ=255)
                                                                  cttttgcGGCGTGTGCATCACCACCCATTCCACTTGCCGGGCATTACGGGGTAGCCCCCGGGGG‑CCGCCACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATcaca                                                                  <  2:2991896/130‑1 (MQ=255)
                                                                   ttttccGGCGTCTGCTTGCGCACCGCATCCACCTGCCGTGTTTTATGGGGTCCCCCGCGGGGG‑CCGCGCGGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCacac                                                                 <  2:2118876/131‑1 (MQ=255)
                                                                        gggCGTGTGCATCCCCCCCCCATCCACAGCCCGCGTTTTATGGGGTAGCGCCGGGGGG‑CCGCGAGGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTacac                                                            <  1:1065952/135‑1 (MQ=255)
                                                                        gggCGCGTGCTTGAGCCCCGCAGCGACAGCCCGCGTATGCGGGGGGCGCGCCGGGGGG‑CCGGGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTacac                                                            <  2:976931/135‑1 (MQ=255)
                                                                                 caggAGCACCGCATCCACAGGCCGCTGATCTTGGGTGAGCGCCCGGGGG‑CCGCGTCGCGCCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTcc                                                   <  2:4105058/133‑1 (MQ=255)
                                                                                  atccccACCGCATCGACTGGCGGGGTATGAGGGGGGTGCGCGGGGGGG‑CCGCGCCGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCt                                                  <  2:4579963/131‑1 (MQ=255)
                                                                                          cGCATCCACATGCCGCGTTTTATGGGTTCGCGCGGGGGGG‑CCGCGCCGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGcc                                          <  1:1176732/136‑1 (MQ=255)
                                                                                            aaTCGACTTGCGGCGGTTTATGGGTTAGAGCCGGGGGG‑CCGCGACGGGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAg                                        <  2:1737745/135‑1 (MQ=255)
                                                                                            aaTCGACATGCCGCGGATGACGGGGTAGAGCCCTGGGG‑CCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAg                                        <  2:1180215/135‑1 (MQ=255)
                                                                                            aaTCCACATCCCGCGTATGACGGGGACGAGCCCGGGGC‑CCGCGACGCGCCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAg                                        <  2:2233712/135‑1 (MQ=255)
                                                                                                          ggATGACGGGGTAGAGCCCGGGGG‑CCGCGCCGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGt                          <  2:2214173/136‑1 (MQ=255)
                                                                                                            ttGATGGGGTAGACCCCTGGGG‑CCGCCACGGGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAg                        <  2:2705385/135‑1 (MQ=255)
                                                                                                                           cgggggg‑CCGCGACGGCCCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGAcgccg         <  1:3585282/133‑1 (MQ=255)
                                                                                                                           ccTGGGG‑CCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGAcgccg         <  2:1821370/136‑1 (MQ=255)
                                                                                                                                gg‑CCGCCCCGCGCCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCttt    <  1:857351/136‑1 (MQ=255)
                                                                                                                                gg‑CCGCCCCGCGCCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCttt    <  1:3216963/136‑1 (MQ=255)
                                                                                                                                   ccGCGCGGCGCCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGc  <  1:350885/136‑1 (MQ=255)
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CGCTGATCCGCCATCGCTGGAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTC‑GCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGG‑CCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGC  >  NC_002947/648116‑648380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑