Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,289,852 Δ1 bp 7.1% coding (1119/2349 nt) PP_1126 → hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,289,8520T. 7.1% 559.8 / 35.3 168coding (1119/2349 nt)PP_1126hydrolase
Reads supporting (aligned to +/- strand):  ref base T (85/71);  new base . (2/10);  total (87/81)
Fisher's exact test for biased strand distribution p-value = 1.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

Only 100 of 168 total aligned reads displayed.

GGGCACCCGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGCCG  >  NC_002947/1289706‑1290000
                                                                                                                                                  |                                                                                                                                                     
gggCACCCGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGc                                                                                                                                                    >  2:2437646/1‑150 (MQ=255)
    aCCCGTTCTATTCCCGGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTAGGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATg                                                                                                                                                >  2:1392426/1‑150 (MQ=255)
      ccGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGcc                                                                                                                                              <  1:1252714/150‑1 (MQ=255)
      ccGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGcc                                                                                                                                              >  2:3854071/1‑150 (MQ=255)
      ccGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGCACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGcc                                                                                                                                              >  2:859484/1‑150 (MQ=255)
       cGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGA‑GGCATTGccat                                                                                                                                            <  1:4492132/150‑3 (MQ=255)
       cGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGA‑GGCATTGccat                                                                                                                                            <  1:2252863/150‑3 (MQ=255)
       cGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGA‑GGCATTGccat                                                                                                                                            <  1:2252665/150‑3 (MQ=255)
        gTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑tc                                                                                                                                           >  1:3507903/1‑150 (MQ=255)
        gTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑tc                                                                                                                                           >  1:2446359/1‑150 (MQ=255)
         ttCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑tcg                                                                                                                                          >  2:2097839/1‑150 (MQ=255)
           cTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑tcgtc                                                                                                                                        <  1:4857857/150‑1 (MQ=255)
           cTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGA‑GGCATTGCCAtcgtc                                                                                                                                        <  1:321362/150‑1 (MQ=255)
           cTAGTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGA‑GGCATTGCCAtcgtc                                                                                                                                        <  2:4689840/150‑1 (MQ=255)
            tATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑tcgtcg                                                                                                                                       >  2:4137172/1‑150 (MQ=255)
            tATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑tcgtcg                                                                                                                                       >  1:1238414/1‑150 (MQ=255)
            tATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑tcgtcg                                                                                                                                       >  2:520160/1‑150 (MQ=255)
             aTTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGc                                                                                                                                      >  2:2430174/1‑150 (MQ=255)
             aTTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGc                                                                                                                                      <  2:4671587/150‑1 (MQ=255)
              ttCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCa                                                                                                                                     >  2:1135301/1‑150 (MQ=255)
               tCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCaa                                                                                                                                    <  2:3258985/150‑1 (MQ=255)
               tCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCaa                                                                                                                                    <  1:180339/150‑1 (MQ=255)
               tCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGA‑GGCATTGCCAtcgtcgttg                                                                                                                                    <  2:157819/150‑4 (MQ=255)
                 ccTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATTGCGAAAGCAACAACGACGA‑GGCATTGCCAtcgtcgttgtt                                                                                                                                  <  2:3803253/150‑6 (MQ=255)
                 ccTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAgg                                                                                                                                  >  2:2258182/1‑150 (MQ=255)
                 ccTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAgg                                                                                                                                  >  2:2460317/1‑150 (MQ=255)
                  cTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAggg                                                                                                                                 >  1:5082858/1‑150 (MQ=255)
                   tGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGgc                                                                                                                                <  2:3480276/150‑1 (MQ=255)
                   tGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGgc                                                                                                                                >  2:3734674/1‑150 (MQ=255)
                   tGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGgc                                                                                                                                <  2:3977947/150‑1 (MQ=255)
                   ggggggTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGgc                                                                                                                                <  2:4845403/149‑1 (MQ=255)
                     ggggTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGgcgc                                                                                                                              >  1:3388770/1‑150 (MQ=255)
                         tGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAAtt                                                                                                                          >  2:2254804/1‑150 (MQ=255)
                         tGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAAtt                                                                                                                          >  2:2254872/1‑150 (MQ=255)
                         tGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAAtt                                                                                                                          >  2:3256188/1‑150 (MQ=255)
                                 gTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAg                                                                                                                  <  1:1504205/150‑1 (MQ=255)
                                  tGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAgg                                                                                                                 <  1:2750989/150‑1 (MQ=255)
                                   ggcggcAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAATGCCTTGCCCGCTGAGGGCTATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAggg                                                                                                                <  1:2832286/150‑1 (MQ=255)
                                      ggcAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGgcg                                                                                                             >  1:1054082/1‑150 (MQ=255)
                                       gcAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGgcgc                                                                                                            <  2:591311/150‑1 (MQ=255)
                                          gACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTgg                                                                                                         <  1:4025208/150‑1 (MQ=255)
                                             ggcgggcgTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGCGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTc                                                                                                      <  2:3633620/150‑1 (MQ=255)
                                             ggcgggcgTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTc                                                                                                      <  2:1887949/150‑1 (MQ=255)
                                             ggcgggcgTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTc                                                                                                      <  1:2859192/150‑1 (MQ=255)
                                             ggcgggcgTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTc                                                                                                      <  2:3633970/150‑1 (MQ=255)
                                                gggcgTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGAc                                                                                                   <  1:520160/150‑1 (MQ=255)
                                                gggcgTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGAc                                                                                                   <  2:4388602/150‑1 (MQ=255)
                                                   cgTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTc                                                                                                <  2:4006980/150‑1 (MQ=255)
                                                    gTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCt                                                                                               >  1:1159517/1‑150 (MQ=255)
                                                       ccTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTAcg                                                                                            >  1:4022064/1‑150 (MQ=255)
                                                        cTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTACTATGGCGAAAGCAACAAAGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTAGACTTCTAcgc                                                                                           >  1:3176768/1‑150 (MQ=255)
                                                           tGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTAcgccgc                                                                                        >  1:3509348/1‑150 (MQ=255)
                                                             tCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCa                                                                                      >  2:4547641/1‑150 (MQ=255)
                                                               gCCGGGCGCGAAGGCATGTTCAAGTCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGATCGACTTCTACGCCGCCAAc                                                                                    >  1:4092981/1‑150 (MQ=255)
                                                                 cGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCt                                                                                  <  1:932823/150‑1 (MQ=255)
                                                                   ggCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGc                                                                                <  1:4753226/150‑1 (MQ=255)
                                                                    gcgcGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCa                                                                               <  1:1128562/150‑1 (MQ=255)
                                                                    gcgcGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCa                                                                               <  2:4159853/150‑1 (MQ=255)
                                                                        gAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGt                                                                           >  2:2661684/1‑150 (MQ=255)
                                                                        gAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGt                                                                           >  2:654973/1‑150 (MQ=255)
                                                                        gAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGt                                                                           >  2:4375796/1‑150 (MQ=255)
                                                                          aGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTAc                                                                         <  1:2080788/150‑1 (MQ=255)
                                                                            gCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCt                                                                       <  2:1897983/150‑1 (MQ=255)
                                                                             cATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTa                                                                      <  1:3513047/150‑1 (MQ=255)
                                                                              aTGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTAt                                                                     <  2:1578084/150‑1 (MQ=255)
                                                                              aTGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTAt                                                                     >  2:1512285/1‑150 (MQ=255)
                                                                              aTGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTAt                                                                     >  1:1341942/1‑150 (MQ=255)
                                                                              aTGTTCAAGGCCTTGCCCGCGGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTAt                                                                     >  1:2590129/1‑150 (MQ=255)
                                                                                gTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATgg                                                                   <  2:3077210/150‑1 (MQ=255)
                                                                                 ttCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGc                                                                  >  1:4842297/1‑150 (MQ=255)
                                                                                 ttCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGc                                                                  >  1:4571584/1‑150 (MQ=255)
                                                                                    aaGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCACTGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCaaa                                                               >  2:2829053/1‑150 (MQ=255)
                                                                                    aaGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCaaa                                                               >  1:4441066/1‑150 (MQ=255)
                                                                                    aaGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCaaa                                                               >  2:2782116/1‑150 (MQ=255)
                                                                                      ggCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAAcc                                                             >  2:2727073/1‑150 (MQ=255)
                                                                                                         gATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAg                                          >  1:2745300/1‑150 (MQ=255)
                                                                                                             cACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGTCAAACCCTGCCGGCCCGCAGTGGAGGgcc                                      <  2:1387056/150‑1 (MQ=255)
                                                                                                             cACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGgcc                                      <  1:1900166/150‑1 (MQ=255)
                                                                                                              aCTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCACGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGgcca                                     >  2:2784032/1‑150 (MQ=255)
                                                                                                              aCTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGgcca                                     >  1:3109501/1‑150 (MQ=255)
                                                                                                              aCTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGgcca                                     >  1:4950972/1‑150 (MQ=255)
                                                                                                              aCTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGgcca                                     >  2:2061041/1‑150 (MQ=255)
                                                                                                                  gCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGgccagcca                                 >  1:3990065/1‑150 (MQ=255)
                                                                                                                   ccGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGgccagcagg                                <  2:244179/150‑4 (MQ=255)
                                                                                                                    cGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCcagccagc                               >  2:1298974/1‑150 (MQ=255)
                                                                                                                    cGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCcagccagc                               >  1:3054341/1‑150 (MQ=255)
                                                                                                                    cGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCcagccagc                               >  1:4107809/1‑150 (MQ=255)
                                                                                                                       tttCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGa                            <  1:4135503/150‑1 (MQ=255)
                                                                                                                         tCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGAtt                          <  2:1819570/150‑1 (MQ=255)
                                                                                                                           tATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTgc                        >  1:3522834/1‑150 (MQ=255)
                                                                                                                             tGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTgcgc                      >  2:2656544/1‑150 (MQ=255)
                                                                                                                              ggCGAAAGCAACAACGACGATGGCAATGCC‑TCCTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCAGCAGCGTACCTATGGCAAACCCAGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTgcgcg                     >  1:1477595/1‑150 (MQ=255)
                                                                                                                                        acaacGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGc           >  2:1023784/1‑150 (MQ=255)
                                                                                                                                         caacGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCt          >  1:3111321/1‑150 (MQ=255)
                                                                                                                                           acgacgATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTgg        <  2:1238414/150‑1 (MQ=255)
                                                                                                                                            cgacga‑GGCATTGCCATCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGa       >  1:3262111/1‑150 (MQ=255)
                                                                                                                                             gacgaTGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGAt      <  2:1600389/150‑1 (MQ=255)
                                                                                                                                               cgaTGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGc    <  1:2460317/150‑1 (MQ=255)
                                                                                                                                               cgaTGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGc    <  1:2097839/150‑1 (MQ=255)
                                                                                                                                                 aTGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGCCg  <  1:293775/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GGGCACCCGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGCCG  >  NC_002947/1289706‑1290000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑