Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,570,510	G→A	8.9%	intergenic (+76/‑112)	pcaF‑I → / → pcaT	beta‑ketoadipyl‑CoA thiolase/alpha‑ketoglutarate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,570,510	0	G	A	8.9%	193.9 / 7.5	69	intergenic (+76/‑112)	pcaF‑I/pcaT	beta‑ketoadipyl‑CoA thiolase/alpha‑ketoglutarate permease
Reads supporting (aligned to +/- strand):  ref base G (31/31);  new base A (3/3);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

GCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTACACCGGCGAAGAACGCAGCAA  >  NC_002947/1570361‑1570659                                                                                                                                                       |                                                                                                                                                      gCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCACGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCcg                                                                                                                                                       <  2:4408187/150‑1 (MQ=255) gCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGACAAGTTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCTCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCcg                                                                                                                                                       <  2:4408445/150‑1 (MQ=255)       gCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAaga                                                                                                                                                 <  1:1677458/150‑1 (MQ=255)       gCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCACCAACAGC‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAaga                                                                                                                                                 <  2:2468021/150‑1 (MQ=255)         aaGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAgg                                                                                                                                               <  2:5084452/150‑1 (MQ=255)                tGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGGATCCCCCGCCAACAGG‑GGTCCACGCCCACACCGGGGGGGGCGGGCGGCCCCCCGCAGCGGccgcgcc                                                                                                                                        >  1:3121550/1‑145 (MQ=255)                      cctggggtgtgGGTGTCGGCAATGGTGGGGGGTGGGCCATCGCGGGGGTTTGACCTGTCCCGGCCTCTTCGCGGGGAAGCCCGCGCCAACTGG‑GATCCACGAAATCACCGGTGGGAGCGGGCTCCCCCGCGAAGAGGCCTCTCCTGTgag                                                                                                                                  <  1:4157683/144‑1 (MQ=255)                      ccATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTgag                                                                                                                                  <  2:5012552/150‑1 (MQ=255)                             tgtgGGTGTCGGCCACGGTGTGGGGTTGGCCATCGCGCGGGTTTGACCTGTACCGGACTCTTCGGGGGGACGCCCGCTCCACCAGG‑GATCCACGCAATCACCTGGGGGCGGGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAg                                                                                                                           <  2:2293711/150‑1 (MQ=255)                                    gtCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCAtt                                                                                                                    <  2:1984153/150‑1 (MQ=255)                                       ggCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGcc                                                                                                                 >  2:3816128/1‑150 (MQ=255)                                          cAATGTATGGCGTTGGCCATCGAGCTGGTTAGACCAGTAACGGCCTCTTCGCGGGGAAGCCCTCACCAAAAGG‑GATACACGCAATAACCGGTGGGAGCGGGCTTCCCCGCGAAGAGGCCGCTCCTGGGAGACAAAAGGCCCATTGCCAGc                                                                                                              >  2:1181951/1‑150 (MQ=255)                                          cAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGc                                                                                                              >  2:1181953/1‑150 (MQ=255)                                          cAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCCCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCACCTGTGAGACATAAGGCCCATTGCCAGc                                                                                                              >  2:1181892/1‑150 (MQ=255)                                                cgggggTTGGCCTTGGCGGGGGTTTGACCTGTACCGGCCTCGTCGCGGGGAAGCCCGCTCCAACCGG‑GATCCCCGAAATCACGGGTGGGCGCGGGCTCCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATa                                                                                                        <  1:3742646/145‑1 (MQ=255)                                                      ttGGCCAGCGCGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGG‑TATCCACGCAATCCCCTGTGGGCGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGaa                                                                                                  <  2:3517709/150‑1 (MQ=39)                                                                     gTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAg                                                                                   <  2:2404338/150‑1 (MQ=255)                                                                       ttGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCCCCTGTGGGCGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGt                                                                                 <  2:1738719/150‑1 (MQ=255)                                                                           ccTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCTACAGG‑GTTCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGTCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATATCTGAACGAGTGTGTTACTAGGTATGt                                                                             <  2:4528012/150‑1 (MQ=255)                                                                                       tctTCGCGGGGAAGCCCGCTCCCACAGGTGATTGCGTGGATC‑CCTGTTGGAGCGGGCTTCCCCACGAAGAGGCCTCTCCTGTGAGACATAATGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCt                                                                 >  2:485337/1‑150 (MQ=18)                                                                                       tctTCGCGGGGAAGCCCGCTCCCACAGGTGATTGCGTGGATC‑CCTGTTGGAGCGGGCTTCCCCACGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCt                                                                 >  2:485582/1‑150 (MQ=18)                                                                                       tctTCGCGGGGAAGCCCGCTCCCACAGGTGATTGCGTGGATC‑CCTGTTGGAGCGGGCTTCCCCACGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCt                                                                 >  2:485466/1‑150 (MQ=18)                                                                                       tctTCGCGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCt                                                                 >  2:4070817/1‑150 (MQ=255)                                                                                        ttgggcgGGGACGCCCGCACCAACCGG‑GACCCCCGCAACCCCGGGTGGGCGCGGGCGGCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTa                                                                <  1:3549236/146‑1 (MQ=255)                                                                                        ctTCGCGGGGAAGCCCGCTCCAACCGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTAGTTCTAGACATACCTa                                                                <  2:3219532/150‑1 (MQ=255)                                                                                        ctTCGCGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTa                                                                <  2:2447336/150‑1 (MQ=255)                                                                                                  aaGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGTGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCa                                                      <  1:799517/150‑1 (MQ=255)                                                                                                   aGCCCGCCCCAACAGG‑GATCCACGCACTCACCTGTGGGTGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAg                                                     <  2:3121550/150‑1 (MQ=255)                                                                                                    gCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAgg                                                    >  1:2921389/1‑150 (MQ=255)                                                                                                     cccGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGa                                                   >  2:2139901/1‑150 (MQ=255)                                                                                                     cccGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGa                                                   <  2:3268721/150‑1 (MQ=255)                                                                                                     cccGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGa                                                   <  2:389887/150‑1 (MQ=255)                                                                                                     cccGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGa                                                   >  1:1815153/1‑150 (MQ=255)                                                                                                     cccGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGa                                                   <  1:2139901/150‑1 (MQ=255)                                                                                                         cTCCAACCGG‑GAGCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTaa                                               <  1:4564843/150‑1 (MQ=255)                                                                                                          tCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCAAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTaaa                                              >  1:4771099/1‑150 (MQ=255)                                                                                                             aaCAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAAccc                                           >  2:603458/1‑150 (MQ=255)                                                                                                             aaCAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAAcac                                           >  1:4294785/1‑148 (MQ=255)                                                                                                              aCAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCg                                          >  1:820995/1‑150 (MQ=255)                                                                                                               cAGG‑GATCCACGCAATCACCTGGGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGt                                         <  1:3704474/150‑1 (MQ=255)                                                                                                                  g‑gATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCAt                                      >  1:914219/1‑150 (MQ=255)                                                                                                                        cACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCt                                 >  1:4316860/1‑150 (MQ=255)                                                                                                                         aCGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTc                                >  2:448233/1‑150 (MQ=255)                                                                                                                         aCGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTc                                >  2:3323574/1‑150 (MQ=255)                                                                                                                         aCGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTc                                >  2:721892/1‑150 (MQ=255)                                                                                                                         aCGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTc                                >  1:834548/1‑150 (MQ=255)                                                                                                                         aCGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTc                                >  2:3751436/1‑150 (MQ=255)                                                                                                                         aCGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTc                                >  2:722248/1‑150 (MQ=255)                                                                                                                            cAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAAc                             <  2:3029196/150‑1 (MQ=255)                                                                                                                             aaTCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAg                                                                                                                           <  2:520574/55‑1 (MQ=255)                                                                                                                             aaTCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAg                                                                                                                           >  1:520574/1‑55 (MQ=255)                                                                                                                                 aCCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTAtt                        >  1:3522921/1‑150 (MQ=255)                                                                                                                                 aCCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTAtt                        >  2:1684551/1‑150 (MQ=255)                                                                                                                                   cTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTac                      >  1:1534925/1‑150 (MQ=255)                                                                                                                                    tgtgGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTaca                     >  1:229255/1‑150 (MQ=255)                                                                                                                                     gtgGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTacac                    <  2:724647/150‑1 (MQ=255)                                                                                                                                     gtgGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTacac                    <  2:5010891/150‑1 (MQ=255)                                                                                                                                      tgGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTACAcc                   >  2:3810596/1‑150 (MQ=255)                                                                                                                                      tgGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTACAcc                   >  2:3545219/1‑150 (MQ=255)                                                                                                                                       gggAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCACCCTATTACACCg                  <  2:1772547/150‑1 (MQ=255)                                                                                                                                       gggAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTACACCg                  <  2:1585944/150‑1 (MQ=255)                                                                                                                                         gAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGt                                                                             >  2:4008307/1‑89 (MQ=255)                                                                                                                                         gAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGt                                                                             <  1:4008307/89‑1 (MQ=255)                                                                                                                                         gAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTACACCGGc                <  2:4092010/150‑1 (MQ=255)                                                                                                                                            ggggCTCCCCCGCTAATTGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTACACCGGCgaa             <  1:1298694/149‑1 (MQ=255)                                                                                                                                             gggCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTACACCGGCgaag            >  2:605907/1‑150 (MQ=255)                                                                                                                                               gCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTACACCGGCgaagaa          >  2:2033340/1‑150 (MQ=255)                                                                                                                                               gCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTACACCGGCgaagaa          >  2:4875161/1‑150 (MQ=255)                                                                                                                                                  tCCCCACGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTACACCGGCGAAGAACgc       <  1:485582/150‑1 (MQ=255)                                                                                                                                                  tCCCCACGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTACACCGGCGAAGAACgc       <  1:485466/150‑1 (MQ=255)                                                                                                                                                  tCCCCACGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTACACCGGCGAAGAACgc       <  1:485337/150‑1 (MQ=255)                                                                                                                                                      cgcgAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTATGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTACACCGGCGAAGAACgcagca   >  2:4125527/1‑150 (MQ=255)                                                                                                                                                       gcgAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTACACCGGCGAAGAACGCAGCaa  >  1:2887059/1‑150 (MQ=255)                                                                                                                                                       gcgAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTACACCGGCGAAGAACGCAGCaa  >  2:3277930/1‑150 (MQ=255)                                                                                                                                                       gcgAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTACACCGGCGAAGAACGCAGCaa  >  2:3277937/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                      GCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACCTCAACCTATTACACCGGCGAAGAACGCAGCAA  >  NC_002947/1570361‑1570659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑