BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,661,525	T→G	7.6%	V52G (GTC→GGC)	PP_1456 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,661,525	0	T	G	7.6%	432.5 / 19.7	132	V52G (GTC→GGC)	PP_1456	membrane protein
Reads supporting (aligned to +/- strand): ref base T (61/61); new base G (6/4); total (67/65)
Fisher's exact test for biased strand distribution p-value = 7.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 133 total aligned reads displayed.

TATCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCTGTGGCTCA  >  NC_002947/1661377‑1661674

tATCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTc                                                                                                                                                      >  2:1337659/1‑150 (MQ=255)

tATCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTc                                                                                                                                                      >  2:2020398/1‑150 (MQ=255)

 aTCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCg                                                                                                                                                     >  1:406354/1‑150 (MQ=255)

    tGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCa                                                                                                                                                  >  1:4121211/1‑150 (MQ=255)

     gATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCAt                                                                                                                                                 <  1:2312327/150‑1 (MQ=255)

       ttGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGc                                                                                                                                               >  1:2331804/1‑150 (MQ=255)

       ttGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGACCAGGGCGCCATga                                                                                                                                               <  1:434302/150‑2 (MQ=255)

       ttGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGACCAGGGCGCCATga                                                                                                                                               <  1:5113425/150‑2 (MQ=255)

       ttGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGACCAGGGCGCCATga                                                                                                                                               <  2:2485441/150‑2 (MQ=255)

          ccTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtgg                                                                                                                                            <  1:4894921/150‑1 (MQ=255)

          ccTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtgg                                                                                                                                            >  1:3089667/1‑150 (MQ=255)

           cTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtggt                                                                                                                                           >  1:4430246/1‑150 (MQ=255)

           cTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtggt                                                                                                                                           >  1:3297714/1‑150 (MQ=255)

            tACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtggtg                                                                                                                                          >  1:1298260/1‑150 (MQ=255)

               aTCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGACa                                                                                                                                       <  2:3171116/150‑1 (MQ=255)

                        gTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTg                                                                                                                              >  1:4218739/1‑150 (MQ=255)

                        gTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTg                                                                                                                              >  1:4218483/1‑150 (MQ=255)

                         tAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGt                                                                                                                             >  1:4235975/1‑150 (MQ=255)

                         tAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGt                                                                                                                             <  2:1066949/150‑1 (MQ=255)

                              cTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTAt                                                                                                                        <  1:2803924/150‑1 (MQ=255)

                                 aTCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTc                                                                                                                     >  2:3376063/1‑150 (MQ=255)

                                       tACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCg                                                                                                               >  2:3783178/1‑150 (MQ=255)

                                       tACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCg                                                                                                               >  1:3970497/1‑150 (MQ=255)

                                       tACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCg                                                                                                               >  2:4212754/1‑150 (MQ=255)

                                       tACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCg                                                                                                               >  2:3783225/1‑150 (MQ=255)

                                       tACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCAGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGACCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCg                                                                                                               >  1:1120547/1‑150 (MQ=255)

                                          gCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAg                                                                                                            <  1:4352635/150‑1 (MQ=255)

                                             ttCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCg                                                                                                         >  1:2274871/1‑150 (MQ=255)

                                                tcCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGcc                                                                                                      >  1:1561526/1‑150 (MQ=255)

                                                  cAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCAt                                                                                                    >  1:3135551/1‑150 (MQ=255)

                                                    gTGCCCCGCATCTGGACCTCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCg                                                                                                  <  2:4515065/150‑1 (MQ=255)

                                                    gTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCg                                                                                                  <  1:2396509/150‑1 (MQ=255)

                                                    gTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCg                                                                                                  <  2:1070622/150‑1 (MQ=255)

                                                         ccGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTg                                                                                             <  2:863184/150‑1 (MQ=255)

                                                         ccGCATCTAGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTg                                                                                             >  2:3556865/1‑150 (MQ=255)

                                                         ccGCATCTAGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTg                                                                                             >  2:3556876/1‑150 (MQ=255)

                                                          cGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTgg                                                                                            >  2:91412/1‑150 (MQ=255)

                                                              tCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAg                                                                                        <  1:364256/150‑1 (MQ=255)

                                                              tCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAg                                                                                        <  1:364089/150‑1 (MQ=255)

                                                                tGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCGgct                                                                                      <  2:1583682/150‑2 (MQ=255)

                                                                   aCATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCa                                                                                   <  1:1277426/150‑1 (MQ=255)

                                                                   aCATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCa                                                                                   <  1:3424277/150‑1 (MQ=255)

                                                                   aCATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCa                                                                                   <  1:850427/150‑1 (MQ=255)

                                                                       catcTGGTCTGGCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGc                                                                               >  2:534807/1‑150 (MQ=255)

                                                                       catcTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGc                                                                               <  2:802024/150‑1 (MQ=255)

                                                                        atcTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCg                                                                              >  2:4326346/1‑150 (MQ=255)

                                                                         tcTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGt                                                                             >  1:1627049/1‑150 (MQ=255)

                                                                         tcTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGt                                                                             >  2:1594533/1‑150 (MQ=255)

                                                                           tGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGttc                                                                           <  1:3417306/150‑1 (MQ=255)

                                                                           tGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGttc                                                                           <  2:1320731/150‑1 (MQ=255)

                                                                           tGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTGGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTCTGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGttc                                                                           <  2:4238917/150‑1 (MQ=255)

                                                                           tGGTCTGCCTGCGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGttc                                                                           <  1:4030015/150‑1 (MQ=255)

                                                                             gTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGttctt                                                                         <  2:377613/150‑1 (MQ=255)

                                                                             gTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGttctt                                                                         <  1:321073/150‑1 (MQ=255)

                                                                                tGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCAt                                                                      >  1:3933264/1‑150 (MQ=255)

                                                                                     ggggCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCg                                                                 <  2:2250538/150‑1 (MQ=255)

                                                                                      gggCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGa                                                                <  1:2459784/150‑1 (MQ=255)

                                                                                       ggcggcCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAg                                                               <  2:4557504/150‑1 (MQ=255)

                                                                                        gcggcCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGc                                                              <  2:4286816/150‑1 (MQ=255)

                                                                                        gcggcCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGc                                                              <  1:1988524/150‑1 (MQ=255)

                                                                                           gcCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGa                                                           <  2:1641356/150‑1 (MQ=255)

                                                                                                      aTCCTGCGCGACATGGTGCAGACCCTTTTTGGTCTTGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTGCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGGGTTCTTCATTTCCGAGCTGATCGTctggctg                                                <  1:3664983/150‑1 (MQ=255)

                                                                                                      aTCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActggctg                                                >  1:4971184/1‑150 (MQ=255)

                                                                                                      aTCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActggctg                                                <  2:282711/150‑1 (MQ=255)

                                                                                                      aTCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActggctg                                                <  2:282695/150‑1 (MQ=255)

                                                                                                        ccTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGgt                                              <  2:1597931/150‑1 (MQ=255)

                                                                                                          tGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCa                                                                                                                                                  >  1:3786732/1‑48 (MQ=255)

                                                                                                                     gTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCAcc                                 >  1:3198940/1‑150 (MQ=255)

                                                                                                                     gTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCAcc                                 >  2:468637/1‑150 (MQ=255)

                                                                                                                     gTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCAcc                                 >  2:468568/1‑150 (MQ=255)

                                                                                                                       gCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCg                               >  2:3072259/1‑150 (MQ=255)

                                                                                                                         aGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTc                             <  2:2476739/150‑1 (MQ=255)

                                                                                                                         aGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTc                             <  2:1746038/150‑1 (MQ=255)

                                                                                                                         aGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTc                             <  1:3464573/150‑1 (MQ=255)

                                                                                                                         aGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTc                             >  2:353180/1‑150 (MQ=255)

                                                                                                                          gACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCg                            <  2:621909/150‑1 (MQ=255)

                                                                                                                          gACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCg                            <  2:621923/150‑1 (MQ=255)

                                                                                                                          gACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCg                            <  2:621994/150‑1 (MQ=255)

                                                                                                                          gACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCg                            <  2:1054319/150‑1 (MQ=255)

                                                                                                                                  ttGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTgc                    >  2:1550418/1‑150 (MQ=255)

                                                                                                                                  accagcATGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCGGCATCACCCGTCGGCCGCTgc                    >  2:3704540/6‑150 (MQ=255)

                                                                                                                                  accagcATGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTgc                    >  2:3704536/6‑150 (MQ=255)

                                                                                                                                  accagcATGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTgc                    >  2:3704641/6‑150 (MQ=255)

                                                                                                                                    ggTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCGTCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTgcgc                  <  1:3246592/150‑1 (MQ=255)

                                                                                                                                    ggTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTgcgc                  >  2:3998533/1‑150 (MQ=255)

                                                                                                                                     gTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGcgcg                 >  1:4002793/1‑150 (MQ=255)

                                                                                                                                     gTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGcgcg                 >  1:1739877/1‑150 (MQ=255)

                                                                                                                                      tCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGa                >  1:4974157/1‑150 (MQ=255)

                                                                                                                                      tCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGa                >  1:2030334/1‑150 (MQ=255)

                                                                                                                                      tCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGa                >  1:141910/1‑150 (MQ=255)

                                                                                                                                      gcATGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGa                >  1:2170366/2‑150 (MQ=255)

                                                                                                                                      gcATGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGa                >  2:4556218/2‑150 (MQ=255)

                                                                                                                                      gcATGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGa                >  1:2170153/2‑150 (MQ=255)

                                                                                                                                        aTGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGAcc              >  1:3685387/1‑150 (MQ=255)

                                                                                                                                        aTGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGAcc              >  1:1513026/1‑150 (MQ=255)

                                                                                                                                         tGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCg             <  1:515553/150‑1 (MQ=255)

                                                                                                                                          ggCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGc            >  2:1274244/1‑150 (MQ=255)

                                                                                                                                          ggCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGc            >  1:375011/1‑150 (MQ=255)

                                                                                                                                                cTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCTGTgg      <  1:1274244/150‑1 (MQ=255)

                                                                                                                                                   gTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCTGTGGCTc   <  2:406354/150‑1 (MQ=255)

TATCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCTGTGGCTCA  >  NC_002947/1661377‑1661674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`