Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,967,041 C→G 5.0% T218T (ACC→ACG) ‡ PP_1762 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,967,0410CG 5.0% 502.8 / 10.3 151T218T (ACC→ACG) ‡PP_1762hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (58/85);  new base G (3/5);  total (61/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

TGTGCTCGACCGACACCTATGCGCAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCG  >  NC_002947/1966893‑1967189
                                                                                                                                                    |                                                                                                                                                    
tgtgCTCGACCGACACCTATGCGCAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCAccc                                                                                                                                                     <  2:4016066/150‑1 (MQ=255)
tgtgCTCGACCGACACCTATGCGCAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCAccc                                                                                                                                                     >  2:4312632/1‑150 (MQ=255)
    cTCGACCGACACCTATGCGCAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTggg                                                                                                                                                 <  2:368841/150‑1 (MQ=255)
    cTCGACCGACACCTATGCGCAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTggg                                                                                                                                                 >  1:408927/1‑150 (MQ=255)
     tCGACCGACACCTATGCGCAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTgggg                                                                                                                                                <  2:2821437/150‑1 (MQ=255)
      cgaccgacACCTATGCGCAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGt                                                                                                                                               <  2:4843129/150‑1 (MQ=255)
       gaccgacACCTATGCGCAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTGTGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGtt                                                                                                                                              >  2:4032549/1‑150 (MQ=255)
         ccgacACCTATGCGCAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCAGGGTGGGGttct                                                                                                                                            <  1:3182496/150‑3 (MQ=255)
         ccgacACCTATGCGCAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGc                                                                                                                                            >  2:3753585/1‑150 (MQ=255)
          cgacACCTATGCGCAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCAGGGTGGGGttctc                                                                                                                                           <  2:3532238/150‑4 (MQ=255)
            acacCTATGCGCAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATGTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGa                                                                                                                                         >  2:3481284/1‑150 (MQ=255)
            acacCTATGCGCAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGa                                                                                                                                         <  1:1334507/150‑1 (MQ=255)
             cacCTATGCGCAGGGGGTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGAc                                                                                                                                        <  2:2307113/150‑1 (MQ=255)
              acCTATGCGCAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGAcc                                                                                                                                       <  1:1655403/150‑1 (MQ=255)
              acCTATGCGCAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGAcc                                                                                                                                       >  1:432876/1‑150 (MQ=255)
                cTATGCGCAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGa                                                                                                                                     <  1:4581286/150‑1 (MQ=255)
                   tGCGCAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGAcc                                                                                                                                       <  1:1361015/145‑1 (MQ=255)
                    gcgcAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTg                                                                                                                                 <  1:2749667/150‑1 (MQ=255)
                     cgcAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGGATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTGCCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTgc                                                                                                                                <  2:2103797/150‑1 (MQ=255)
                        aGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCa                                                                                                                             >  1:2370566/1‑150 (MQ=255)
                          gggTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGttctc                                                                                                                                           >  2:3320810/1‑131 (MQ=255)
                          gggTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAgg                                                                                                                           <  1:4623004/150‑1 (MQ=255)
                          gggTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAgg                                                                                                                           >  2:501785/1‑150 (MQ=255)
                                ccTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATc                                                                                                                     <  1:2409226/150‑1 (MQ=255)
                                 cTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCt                                                                                                                    <  2:2726833/150‑1 (MQ=255)
                                 cTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCt                                                                                                                    >  1:914177/1‑150 (MQ=255)
                                  tGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGGGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTg                                                                                                                   <  1:3050858/150‑1 (MQ=255)
                                  tGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTg                                                                                                                   >  1:2948827/1‑150 (MQ=255)
                                  tGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTg                                                                                                                   >  1:391114/1‑150 (MQ=255)
                                   gaagaaGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGc                                                                                                                  <  2:249723/150‑1 (MQ=255)
                                   gaagaaGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGc                                                                                                                  <  1:602605/150‑1 (MQ=255)
                                     agaagGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCaa                                                                                                                <  2:2543664/150‑1 (MQ=255)
                                        agGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAagag                                                                                                             <  2:4527480/150‑1 (MQ=255)
                                         gggCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGt                                                                                                            >  1:412406/1‑150 (MQ=255)
                                          ggCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGtt                                                                                                           <  1:266953/150‑1 (MQ=255)
                                          ggCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGtt                                                                                                           <  1:5069471/150‑1 (MQ=255)
                                           gcgcAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTa                                                                                                          >  2:612301/1‑150 (MQ=255)
                                           gcgcAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTa                                                                                                          >  2:3559893/1‑150 (MQ=255)
                                            cgcAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTAc                                                                                                         <  1:3900511/150‑1 (MQ=255)
                                            cgcAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTAc                                                                                                         <  2:1994312/150‑1 (MQ=255)
                                              cAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGa                                                                                                       <  1:1951134/150‑1 (MQ=255)
                                               aaGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGAc                                                                                                      >  1:1735775/1‑150 (MQ=255)
                                               aaGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGAc                                                                                                      <  1:4775487/150‑1 (MQ=255)
                                                  cGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGc                                                                                                   <  2:2487043/150‑1 (MQ=255)
                                                        aTGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCAtgttc                                                                                             <  1:3122068/150‑1 (MQ=255)
                                                               ggCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCAtgttcctgttcc                                                                                      <  2:1575537/150‑1 (MQ=255)
                                                                  tGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTgg                                                                                   >  1:1567509/1‑150 (MQ=255)
                                                                  tGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTgg                                                                                   >  1:4542302/1‑150 (MQ=255)
                                                                  tGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTgg                                                                                   >  2:1722670/1‑150 (MQ=255)
                                                                    aaGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAg                                                                                 >  1:4754904/1‑150 (MQ=255)
                                                                       atgcatgcCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGAGCTGCATGAGTTACGACTGCATGTTCCTGTTCCTGGAgcgc                                                                              >  1:4145372/1‑150 (MQ=255)
                                                                        tgcatgcCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCt                                                                             <  2:2177460/150‑1 (MQ=255)
                                                                        tgcatgcCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCt                                                                             <  1:2308484/150‑1 (MQ=255)
                                                                           atgcCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCtgca                                                                          <  1:614040/150‑1 (MQ=255)
                                                                            tgcCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCtgcat                                                                         >  1:494488/1‑150 (MQ=255)
                                                                             gcCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCtgcatg                                                                        >  1:2124840/1‑150 (MQ=255)
                                                                              cccAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCtgcatgc                                                                       <  2:3064982/150‑1 (MQ=255)
                                                                               ccAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCtgcatgca                                                                      >  1:757647/1‑150 (MQ=255)
                                                                                cAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAg                                                                     >  1:3589013/1‑150 (MQ=255)
                                                                                 aGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCCTGTTCCTGTTCCTGGAGCGCTGCATGCAGc                                                                    >  2:4310178/1‑150 (MQ=255)
                                                                                  ttATGACGATGCGCACCCGAGGGATGCGCTTGAAATCATCTGTACCCTGGCCGGCGAGAACCACACCCTGGGTTTGATGACCGAGTTTCGCAGTGCGATATGCAAGAGTTACTACTTCATGTTCCTGTTCCTGGAGATCTGCATGCATCt                                                                   <  2:3699514/149‑1 (MQ=255)
                                                                                  gTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCt                                                                   <  2:3996714/150‑1 (MQ=255)
                                                                                     tGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGa                                                                <  1:2161506/150‑1 (MQ=255)
                                                                                      gACGATGTGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGaa                                                               <  1:1494684/150‑1 (MQ=255)
                                                                                      gACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGTCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGaa                                                               <  1:3718488/150‑1 (MQ=255)
                                                                                      gACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGaa                                                               >  2:4882162/1‑150 (MQ=255)
                                                                                      gACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGaa                                                               >  1:4430877/1‑150 (MQ=255)
                                                                                      gACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGaa                                                               >  1:4430828/1‑150 (MQ=255)
                                                                                        cGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAgg                                                             >  1:1199323/1‑150 (MQ=255)
                                                                                          aTGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGgc                                                           <  1:1194323/150‑1 (MQ=255)
                                                                                          aTGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGgc                                                           <  2:4132451/150‑1 (MQ=255)
                                                                                            gcgcACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGgcgc                                                         <  1:3596120/150‑1 (MQ=255)
                                                                                              gcACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCAGGGTGGGGttct                                                                                                                                            >  2:4978998/1‑63 (MQ=255)
                                                                                                 cccGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCa                                                    >  2:2785016/1‑150 (MQ=255)
                                                                                                  ccGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCaa                                                   >  1:340173/1‑150 (MQ=255)
                                                                                                  ccGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCaa                                                   <  1:1559856/150‑1 (MQ=255)
                                                                                                     tGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGc                                                <  2:1344408/150‑1 (MQ=255)
                                                                                                     tGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGc                                                <  2:1535220/150‑1 (MQ=255)
                                                                                                     tGGGAGGCGCTGGAAATCATCTGGACCCTGGCCGGCGAGCACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGGTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGc                                                <  2:3457843/150‑1 (MQ=255)
                                                                                                                      catcTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGtt                               <  2:4928604/150‑1 (MQ=255)
                                                                                                                      catcTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGtt                               <  1:3273754/150‑1 (MQ=255)
                                                                                                                      catcTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGtt                               <  1:4683502/150‑1 (MQ=255)
                                                                                                                       atcTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGCATAcggcctt                                   >  1:4852266/1‑143 (MQ=255)
                                                                                                                             aCCCTGTCCGGCGAGAACACCACCCTGGGGTTGCGGACCGAGTTGCGCAGTGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCATCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGTCTGCg                        <  2:2807044/150‑1 (MQ=255)
                                                                                                                             aCCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCg                        <  2:412406/150‑1 (MQ=255)
                                                                                                                             aCCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCg                        <  1:4312632/150‑1 (MQ=255)
                                                                                                                               ccTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTAt                                           >  1:2759527/1‑129 (MQ=255)
                                                                                                                               ccTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTAt                                           <  2:2759457/129‑1 (MQ=255)
                                                                                                                                 tGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCt                    <  1:3753585/150‑1 (MQ=255)
                                                                                                                                   gCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGa                  <  2:228163/150‑1 (MQ=255)
                                                                                                                                     cGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATc                <  2:2228910/150‑1 (MQ=255)
                                                                                                                                         cgcaaCCCCAGGGTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCtt            >  2:1126716/4‑150 (MQ=255)
                                                                                                                                           gaACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTc          <  1:501785/150‑1 (MQ=255)
                                                                                                                                           gaACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTc          >  2:4574084/1‑150 (MQ=255)
                                                                                                                                           gaACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTc          <  2:4634803/150‑1 (MQ=255)
                                                                                                                                                ccACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTa     <  2:3775388/150‑1 (MQ=255)
                                                                                                                                                ccACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTa     <  2:1181576/150‑1 (MQ=255)
                                                                                                                                                   cccTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCG  >  1:2856058‑M1/1‑150 (MQ=255)
                                                                                                                                                    ccTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATgttcctgttcct                                                                                     >  2:4529315/1‑66 (MQ=255)
                                                                                                                                                    ccTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATgttcctgttcct                                                                                     <  1:4529315/66‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGTGCTCGACCGACACCTATGCGCAGGGGTTCCCTGAAGAAGGGCGCAAGCGGGCGATGAAGTGGCTGAAGATGCATGCCCAGTATGACGATGCGCACCCGTGGGAGGCGCTGGAAATCATCTGTACCCTGGCCGGCGAGAACCCCACCCTGGGGTTGCGGACCGAGTTGCGCAGGGCGATCTGCAAGAGTTACGACTGCATGTTCCTGTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCG  >  NC_002947/1966893‑1967189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑