BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,310,601	C→A	5.3%	G145V (GGC→GTC) ‡	PP_2031 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,310,601	0	C	A	5.3%	443.8 / 10.3	133	G145V (GGC→GTC) ‡	PP_2031	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (62/64); new base A (4/3); total (66/67)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 135 total aligned reads displayed.

GCGGGCGCGGCGCAGGGCCAGGCCGAGGCTGTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGACGAGGATGAAGATCGGCCGC  >  NC_002947/2310453‑2310750

gcgGGCGCGGCGCAGGGCCAGGCCGAGGCTGTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGATTGATTTCGTGGTGTTCGTTGTCGcc                                                                                                                                                      <  2:2761030/150‑1 (MQ=255)

  gggCGCGGCGCAGGGCCAGGCCGAGGCTGTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGa                                                                                                                                                    <  1:1224003/150‑1 (MQ=255)

  gggCGCGGCGCAGGGCCAGGCCGAGGCTGTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGa                                                                                                                                                    >  2:1140963/1‑150 (MQ=255)

   ggcgcggcgcAGGGCCAGGCCGAGGCTGTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGaa                                                                                                                                                   <  2:4887845/150‑1 (MQ=255)

   ggcgcggcgcAGGGCCAGGCCGAGGCTGTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGaa                                                                                                                                                   <  2:1226560/150‑1 (MQ=255)

        ggcgcAGGGCCAGGCCGAGGCTGTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCa                                                                                                                                              <  2:1713801/150‑1 (MQ=255)

        ggcgcAGGGCCAGGCCGAGGCTGTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGGCGACAACGAACACCa                                                                                                                                              <  2:3490505/150‑1 (MQ=255)

         gcgcAGGGCCAGGCCGAGGCTGTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGGCGACAACGAACACCac                                                                                                                                             <  2:1224257/150‑2 (MQ=255)

              gggCCAGGCCGAGGCTGTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGgccgc                                                                                                                                        <  2:2077741/150‑1 (MQ=255)

               ggCCAGGCCGAGGCTGTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGgccgcc                                                                                                                                       >  2:4158066/1‑150 (MQ=255)

                 ccAGGCCGAGGCTGTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGa                                                                                                                                     <  2:568566/150‑1 (MQ=255)

                     gCCGAGGCTGTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCg                                                                                                                                 >  2:4224959/1‑150 (MQ=255)

                     gCCGAGGCTGTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCg                                                                                                                                 <  1:1153746/150‑1 (MQ=255)

                      ccGAGGCTGTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCgg                                                                                                                                <  1:3944453/150‑1 (MQ=255)

                      ccGAGGCTGTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCgg                                                                                                                                <  2:86407/150‑1 (MQ=255)

                           gCTGTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGtt                                                                                                                           <  1:3056469/150‑1 (MQ=255)

                           gCTGTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGtt                                                                                                                           <  1:976419/150‑1 (MQ=255)

                              gTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGgccg                                                                                                                                         >  2:1424466/1‑133 (MQ=255)

                              gTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGgccg                                                                                                                                         <  1:1424466/133‑1 (MQ=255)

                                cGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGc                                                                                                                      >  1:4888620/1‑150 (MQ=255)

                                    cccGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAg                                                                                                                  >  2:2593137/1‑150 (MQ=255)

                                     ccGGGTATGGCTTCGGTGTGCCGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAgc                                                                                                                 >  2:4642556/1‑150 (MQ=255)

                                        ggTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCt                                                                                                              <  1:3383757/150‑1 (MQ=255)

                                        ggTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCt                                                                                                              <  1:3384425/150‑1 (MQ=255)

                                               cTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGc                                                                                                       <  2:789443/150‑1 (MQ=255)

                                                 tCGGTGTGCAGTGACTGGTTGACCTTTGCCACGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCg                                                                                                     <  2:3794469/150‑1 (MQ=255)

                                                    gtgtGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAAc                                                                                                  <  2:3967193/150‑1 (MQ=255)

                                                     tgtgCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACa                                                                                                 >  1:2290835/1‑150 (MQ=255)

                                                       tgCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAgc                                                                                               <  1:1973871/150‑1 (MQ=255)

                                                        gCAGTGACTGTTTGACCTTTGCCAAGCGGTTGAGCATTTGAAGCATGCTTTGCTTTCTGAGTAGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCTAACACAAGGCCGCCGATGATCTCGTTGTGGCCCAGCTCTGCCCAGCCGAACatct                                                                                              <  2:778143/150‑4 (MQ=255)

                                                            tGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGcgccgc                                                                                          >  2:1527806/1‑150 (MQ=255)

                                                             gACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGGTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCGCAGCGCTGCCCAGCCGAACAGCGCCGCa                                                                                         >  2:2796676/1‑150 (MQ=255)

                                                                  gTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTg                                                                                    >  1:4457176/1‑150 (MQ=255)

                                                                  gTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTg                                                                                    >  1:3644977/1‑150 (MQ=255)

                                                                      aCCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGTTTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCTGCCTGGgcc                                                                                >  2:4944419/1‑150 (MQ=255)

                                                                      aCCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGgcc                                                                                >  2:1761775/1‑150 (MQ=255)

                                                                        cTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGgccgc                                                                              <  2:3309880/150‑1 (MQ=255)

                                                                        cTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGgccgc                                                                              <  2:1528184/150‑1 (MQ=255)

                                                                         tttGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGgccgcc                                                                             >  1:83952/1‑150 (MQ=255)

                                                                              cAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCAc                                                                        >  1:167629/1‑150 (MQ=255)

                                                                                  cGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGAc                                                                    <  1:3858407/150‑1 (MQ=255)

                                                                                  cGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGAc                                                                    <  1:4271184/150‑1 (MQ=255)

                                                                                    gTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACtt                                                                  <  2:1864757/150‑1 (MQ=255)

                                                                                    gTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACtt                                                                  <  2:305064/150‑1 (MQ=255)

                                                                                     ttGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTg                                                                 <  2:2276089/150‑1 (MQ=255)

                                                                                     ttGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTg                                                                 <  2:1798261/150‑1 (MQ=255)

                                                                                     ttGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTACCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTg                                                                 <  1:3083107/150‑1 (MQ=255)

                                                                                      tGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGa                                                                <  1:3599399/150‑1 (MQ=255)

                                                                                        aGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACCGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAAc                                                              >  2:225262/1‑150 (MQ=255)

                                                                                        aGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAAc                                                              >  2:3368382/1‑150 (MQ=255)

                                                                                            gTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCa                                                          <  2:2615120/150‑1 (MQ=255)

                                                                                            gTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCa                                                          >  1:4192564/1‑150 (MQ=255)

                                                                                            gTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCa                                                          <  2:4755257/150‑1 (MQ=255)

                                                                                            gTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCa                                                          <  2:4755244/150‑1 (MQ=255)

                                                                                            gTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCa                                                          <  2:4755284/150‑1 (MQ=255)

                                                                                               gcagcaGGCTGTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGcc                                                       <  1:4570951/150‑1 (MQ=255)

                                                                                                  gcagGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTg                                                    >  2:2164128/1‑150 (MQ=255)

                                                                                                   cagGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGa                                                   <  1:2525984/150‑1 (MQ=255)

                                                                                                     ggCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGc                                                 >  2:2607926/1‑150 (MQ=255)

                                                                                                       cTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCg                                               <  1:3264030/150‑1 (MQ=255)

                                                                                                         ttgcttgcTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCgaa                                             <  1:1194166/150‑1 (MQ=255)

                                                                                                         ttgcttgcTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCgaa                                             <  2:760442/150‑1 (MQ=255)

                                                                                                         ttgcttgcTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCgaa                                             <  1:697188/150‑1 (MQ=255)

                                                                                                           gcttgctGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCgaaga                                           >  2:4598259/1‑150 (MQ=255)

                                                                                                             ttgctGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGaaa                                         >  1:4348411/1‑150 (MQ=255)

                                                                                                             ttgctGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGaaa                                         >  1:4463331/1‑150 (MQ=255)

                                                                                                             ttgctGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGaaa                                         >  1:654593/1‑150 (MQ=255)

                                                                                                              tgctgcGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGaaaa                                        <  2:4646976/150‑1 (MQ=255)

                                                                                                              tgctgcGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGaaaa                                        <  1:2484283/150‑1 (MQ=255)

                                                                                                                    gTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGtt                                  <  1:45008/150‑1 (MQ=255)

                                                                                                                         ggCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCtt                             >  2:288772/1‑150 (MQ=255)

                                                                                                                          gCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCACCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCttt                            >  1:441133/1‑150 (MQ=255)

                                                                                                                           cTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTg                           >  1:1430087/1‑150 (MQ=255)

                                                                                                                           cTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTg                           >  1:4622567/1‑150 (MQ=255)

                                                                                                                           cTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTg                           <  1:2818730/150‑1 (MQ=255)

                                                                                                                            ttGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGc                          >  1:300891/1‑150 (MQ=255)

                                                                                                                            ttGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGc                          >  2:4013254/1‑150 (MQ=255)

                                                                                                                              gATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTc                        >  2:3638122/1‑150 (MQ=255)

                                                                                                                               aTTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCTCAGCCTGGGCCGCCAGCACTGACTTGAACATCAGCCCTGAGCCGAAGCAAAGCCACCGGCTTTTCttc                       <  1:1759531/150‑3 (MQ=255)

                                                                                                                                    gtggtgTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCTCGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGACGAg                  >  2:4155239/1‑150 (MQ=255)

                                                                                                                                    gtggtgTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGCAAAGCCAACGGCTTTTCttcg                      <  2:3082596/146‑4 (MQ=255)

                                                                                                                                    gtggtgTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGCAAAGCCAACGGCTTTTCttcg                      >  1:3082596/1‑143 (MQ=255)

                                                                                                                                    ctggtgTTCGGCGACAACGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGGACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGACGAg                  >  2:4938630/2‑150 (MQ=255)

                                                                                                                                    ctggtgTTCGGCGACAACGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGACGAg                  >  1:1794736/2‑150 (MQ=255)

                                                                                                                                     tggtgTTCGGCGACAACGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGaaaa                                        <  2:3549618/127‑1 (MQ=255)

                                                                                                                                     tggtgTTCGGCGACAACGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGACGAgg                 >  2:1616377/1‑150 (MQ=255)

                                                                                                                                     gggtgTTCGGTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGACGAgg                 <  2:4053800/149‑1 (MQ=255)

                                                                                                                                      ggtgTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGACGAGGa                >  2:3748528/1‑150 (MQ=255)

                                                                                                                                        tgtTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGGACATCAGCCCGGAGCCGAAGAAAAGCCGGTGGCGTTGCTCGACGAGGATg              >  1:3976289/1‑150 (MQ=255)

                                                                                                                                         gtTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGACGAGGATGa             <  1:588893/150‑1 (MQ=255)

                                                                                                                                         gtTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGACGAGGATGa             >  1:3697601/1‑150 (MQ=255)

                                                                                                                                            cGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGACGAGGATGAAGa          <  2:2996652/150‑1 (MQ=255)

                                                                                                                                             gTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGACGAGGATGAAGAt         >  1:1772243/1‑150 (MQ=255)

                                                                                                                                             gTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGACGAGGATGAAGAt         >  2:3298184/1‑150 (MQ=255)

                                                                                                                                              ttGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTTTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGACGAGGATGAAGATc        >  1:136583/1‑150 (MQ=255)

                                                                                                                                              ttGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGACGAGGATGAAGATc        <  1:1527806/150‑1 (MQ=255)

                                                                                                                                              ttGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGACGAGGATGAAGATc        >  1:4871764/1‑150 (MQ=255)

                                                                                                                                                 tCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGACGAGGATGAAGATCGGc     >  2:3164578/1‑150 (MQ=255)

                                                                                                                                                  cGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGACGAGGATGAAGATCGGcc    <  2:4192564/150‑1 (MQ=255)

                                                                                                                                                    ccGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGACGAGGATGAAGATCGGCCGc  >  2:4325723/1‑150 (MQ=255)

GCGGGCGCGGCGCAGGGCCAGGCCGAGGCTGTCGGCCCCGGGTATGGCTTCGGTGTGCAGTGACTGGTTGACCTTTGCCAAGCGGTTGAGCAGTTGCAGCAGGCTTTGCTTGCTGCGTCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGACGAGGATGAAGATCGGCCGC  >  NC_002947/2310453‑2310750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`