BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,482,742	G→A	7.0%	R190R (CGC→CGT)	PP_2175 ←	3‑oxoacyl‑ACP reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,482,742	0	G	A	7.0%	656.9 / 25.9	215	R190R (CGC→CGT)	PP_2175	3‑oxoacyl‑ACP reductase
Reads supporting (aligned to +/- strand): ref base G (108/92); new base A (10/5); total (118/97)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 225 total aligned reads displayed.

GGGCCGACAGGCAATCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACT  >  NC_002947/2482593‑2482891

gggCCGACAGGCAATCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTcg                                                                                                                                                       >  1:383889/1‑150 (MQ=255)

    cGACAGGCAATCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCgcggc                                                                                                                                                   >  2:5001699/1‑150 (MQ=255)

     gACGGGCAATCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCgcggcg                                                                                                                                                  >  2:4888958/1‑150 (MQ=255)

      aCAGGCAATCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGgcgc                                                                                                                                                 >  2:3364492/1‑150 (MQ=255)

        aGGCACTCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGGGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCACTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTg                                                                                                                                               >  2:473888/1‑150 (MQ=255)

        aGGCAATCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTg                                                                                                                                               >  1:1574693/1‑150 (MQ=255)

          gCAATCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCACTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGtt                                                                                                                                             >  2:633204/1‑150 (MQ=255)

           cAATCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTc                                                                                                                                            <  1:825009/150‑1 (MQ=255)

           cAATCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTc                                                                                                                                            >  2:1976784/1‑150 (MQ=255)

            aaTCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCg                                                                                                                                           >  2:1729849/1‑150 (MQ=255)

             aTCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCgg                                                                                                                                          >  2:3694687/1‑150 (MQ=255)

              tCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGt                                                                                                                                         >  1:388026/1‑150 (MQ=255)

              tCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGt                                                                                                                                         >  2:2437475/1‑150 (MQ=255)

              tCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGt                                                                                                                                         <  1:68370/150‑1 (MQ=255)

              tCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGt                                                                                                                                         >  2:3767777/1‑150 (MQ=255)

               cAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGtg                                                                                                                                        >  2:1639756/1‑150 (MQ=255)

                aaaCGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGtgg                                                                                                                                       >  1:4591907/1‑150 (MQ=255)

                  aCGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGtggct                                                                                                                                     <  2:287558/150‑1 (MQ=255)

                      tGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCa                                                                                                                                 >  1:2101824/1‑150 (MQ=255)

                           gtCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTg                                                                                                                            >  2:3192805/1‑150 (MQ=255)

                            tCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGt                                                                                                                           <  2:2324998/150‑1 (MQ=255)

                            tCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGt                                                                                                                           <  2:2797889/150‑1 (MQ=255)

                            tCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGt                                                                                                                           <  1:5120767/150‑1 (MQ=255)

                              aaGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCg                                                                                                                         >  1:28492/1‑150 (MQ=255)

                              aaGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCg                                                                                                                         >  2:3106524/1‑150 (MQ=255)

                                gccgccATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGAt                                                                                                                       >  1:3567125/1‑150 (MQ=255)

                                gccgccATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGAt                                                                                                                       >  1:250025/1‑150 (MQ=255)

                                gccgccATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGAt                                                                                                                       >  2:4972170/1‑150 (MQ=255)

                                    ccATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAg                                                                                                                   >  1:494521/1‑150 (MQ=255)

                                     cATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGc                                                                                                                  >  2:367355/1‑150 (MQ=255)

                                         gACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCggg                                                                                                              >  2:2228213/1‑150 (MQ=255)

                                           ccTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGgga                                                                                                            <  1:4530007/150‑1 (MQ=255)

                                           ccTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGgga                                                                                                            <  2:4589651/150‑1 (MQ=255)

                                            cTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGggag                                                                                                           >  2:3538604/1‑150 (MQ=255)

                                            cTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGggag                                                                                                           >  2:2923517/1‑150 (MQ=255)

                                            cTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGggag                                                                                                           >  1:1162606/1‑150 (MQ=255)

                                                  gTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACgtt                                                                                                     <  2:1181739/150‑1 (MQ=255)

                                                   ttCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACgttg                                                                                                    >  1:324749/1‑150 (MQ=255)

                                                   ttCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACgttg                                                                                                    <  2:4771390/150‑1 (MQ=255)

                                                    tCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACgttgt                                                                                                   <  2:5117469/150‑1 (MQ=255)

                                                     cggCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACgttgtt                                                                                                  <  2:138447/148‑1 (MQ=255)

                                                     cTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACgttgtt                                                                                                  <  2:4194072/150‑1 (MQ=255)

                                                         ccGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATc                                                                                              <  2:2493721/150‑1 (MQ=255)

                                                         ccGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATc                                                                                              <  1:4661142/150‑1 (MQ=255)

                                                         ccGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATc                                                                                              <  1:4030761/150‑1 (MQ=255)

                                                         ccGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATc                                                                                              >  2:579635/1‑150 (MQ=255)

                                                           tgTGATGTAGGCCGCACCGTCTGATGCCAGGAAGGCAACTGTTGCGGCAATTTCTTCGCTTTTGCCATAACGCTGCATAGGCACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCAtgcg                                                                                            >  2:4801200/2‑147 (MQ=255)

                                                           ggTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCt                                                                                            >  1:931152/1‑150 (MQ=255)

                                                           ggTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCt                                                                                            >  2:4596337/1‑150 (MQ=255)

                                                                tGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGt                                                                                       >  2:1176212/1‑150 (MQ=255)

                                                                 gTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGtt                                                                                      >  1:1031093/1‑150 (MQ=255)

                                                                  tAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTg                                                                                     >  2:4532612/1‑150 (MQ=255)

                                                                       aGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTcccc                                                                                >  2:2660689/1‑150 (MQ=255)

                                                                       aGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGAGCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTcccc                                                                                >  2:1370506/1‑150 (MQ=255)

                                                                         cgcCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCgg                                                                              <  2:3068955/150‑1 (MQ=255)

                                                                           ccTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCg                                                                            >  1:2436403/1‑150 (MQ=255)

                                                                             ttCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGaa                                                                          >  1:2232446/1‑150 (MQ=255)

                                                                                gCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTg                                                                       <  1:4369594/150‑1 (MQ=255)

                                                                                gCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGAGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTg                                                                       <  1:1043367/150‑1 (MQ=255)

                                                                                 cTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTgg                                                                      >  2:4566020/1‑150 (MQ=255)

                                                                                   ggCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTc                                                                    <  1:1529631/150‑1 (MQ=255)

                                                                                   ggCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTc                                                                    <  2:4248926/150‑1 (MQ=255)

                                                                                      gagaAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGc                                                                 >  2:3897568/1‑150 (MQ=255)

                                                                                      gagaAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAAGTGGTCGGc                                                                 >  2:3897731/1‑150 (MQ=255)

                                                                                       agaAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCg                                                                >  2:4119328/1‑150 (MQ=255)

                                                                                         aaaCGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGaa                                                              <  2:528389/150‑1 (MQ=255)

                                                                                         aaaCGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGaa                                                              >  1:1482490/1‑150 (MQ=255)

                                                                                         aaaCGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGaa                                                              <  1:2604111/150‑1 (MQ=255)

                                                                                                aTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTg                                                       <  1:4832023/150‑1 (MQ=255)

                                                                                                aTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTg                                                       <  1:1458992/150‑1 (MQ=255)

                                                                                                aTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTg                                                       <  1:50015/150‑1 (MQ=255)

                                                                                                aTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTg                                                       <  1:3088704/150‑1 (MQ=255)

                                                                                                aTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTg                                                       <  1:1729849/150‑1 (MQ=255)

                                                                                                           gATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGc                                            <  1:3670273/150‑1 (MQ=255)

                                                                                                                 cTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGcc                                      >  2:4301726/1‑150 (MQ=255)

                                                                                                                  tCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGccc                                     >  2:4003052/1‑150 (MQ=255)

                                                                                                                    gCAGGTGCCATAGCGTTTGAGCGGCTCGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGTGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGa                                   <  1:2704822/150‑1 (MQ=255)

                                                                                                                      aGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGc                                 <  1:1695336/150‑1 (MQ=255)

                                                                                                                        gTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGACGATTTTGGTGAATGCGGCCAGGCCCGAGCgg                               >  1:1091990/1‑150 (MQ=255)

                                                                                                                        gTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCgg                               <  1:620361/150‑1 (MQ=255)

                                                                                                                        gTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCgg                               >  1:95606/1‑150 (MQ=255)

                                                                                                                         tGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGa                              >  2:2007967/1‑150 (MQ=255)

                                                                                                                           ccATAGCGTATGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCAGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAac                            >  2:5000213/1‑150 (MQ=255)

                                                                                                                             aTAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGACTTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAacac                          >  1:4550592/1‑150 (MQ=255)

                                                                                                                             aTAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAacac                          >  2:3132642/1‑150 (MQ=255)

                                                                                                                              tAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACt                         >  2:3529910/1‑150 (MQ=255)

                                                                                                                               aGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTg                        <  2:1033927/150‑1 (MQ=255)

                                                                                                                               aGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTg                        >  2:4674006/1‑150 (MQ=255)

                                                                                                                                 cGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATGCTCTCGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCa                      <  2:1751565/150‑1 (MQ=255)

                                                                                                                                   tttGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGa                    <  1:161630/150‑1 (MQ=255)

                                                                                                                                   tttGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGa                    <  1:3853730/150‑1 (MQ=255)

                                                                                                                                      gAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGt                 <  1:3227938/150‑1 (MQ=255)

                                                                                                                                      cataggCACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGt                 >  1:4394952/5‑150 (MQ=255)

                                                                                                                                          ggCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCggg             <  1:3364492/150‑1 (MQ=255)

                                                                                                                                           gCACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGTCGGGa            >  2:1596471/1‑150 (MQ=255)

                                                                                                                                           gCACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGTCGGGa            <  2:2597248/150‑1 (MQ=255)

                                                                                                                                             aCGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAAc          >  1:2919745/1‑150 (MQ=255)

                                                                                                                                               gCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGTCGGGAACat        >  1:4169167/1‑149 (MQ=255)

                                                                                                                                                cTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGTCGGGAACatt       <  1:1692348/150‑3 (MQ=255)

                                                                                                                                                     gcggcgCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACt  <  1:3694687/150‑1 (MQ=255)

GGGCCGACAGGCAATCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACT  >  NC_002947/2482593‑2482891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`