BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,482,801	T→G	6.7%	R171R (AGG→CGG) ‡	PP_2175 ←	3‑oxoacyl‑ACP reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,482,801	0	T	G	6.7%	625.5 / 26.6	195	R171R (AGG→CGG) ‡	PP_2175	3‑oxoacyl‑ACP reductase
Reads supporting (aligned to +/- strand): ref base T (75/107); new base G (8/5); total (83/112)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01

Only 100 of 203 total aligned reads displayed.

GGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCA  >  NC_002947/2482652‑2482950

ggTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCt                                                                                                                                                       >  1:931231/1‑150 (MQ=255)

ggTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCt                                                                                                                                                       >  2:4596337/1‑150 (MQ=255)

     tGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGt                                                                                                                                                  <  1:861693/150‑1 (MQ=255)

     tGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGt                                                                                                                                                  >  2:1176337/1‑150 (MQ=255)

      gTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGtt                                                                                                                                                 >  1:1031093/1‑150 (MQ=255)

      gTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGtt                                                                                                                                                 <  2:200780/150‑1 (MQ=255)

      gTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGtt                                                                                                                                                 <  1:3713576/150‑1 (MQ=255)

       tAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTg                                                                                                                                                >  2:4532612/1‑150 (MQ=255)

            aGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTcccc                                                                                                                                           >  2:1370619/1‑150 (MQ=255)

                   tCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGGAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAAt                                                                                                                                    <  1:2288547/150‑1 (MQ=255)

                     gCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTg                                                                                                                                  <  1:4369632/150‑1 (MQ=255)

                      cTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTgg                                                                                                                                 >  2:4566020/1‑150 (MQ=255)

                       tGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGt                                                                                                                                >  1:1044687/1‑150 (MQ=255)

                        ggCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTc                                                                                                                               <  1:1529631/150‑1 (MQ=255)

                         gCGAGAACCGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCg                                                                                                                              >  2:4297106/1‑150 (MQ=255)

                         gCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCg                                                                                                                              <  2:3745885/150‑1 (MQ=255)

                         gCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCg                                                                                                                              <  1:3846295/150‑1 (MQ=255)

                         gCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCg                                                                                                                              <  2:1107289/150‑1 (MQ=255)

                           gagaAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGc                                                                                                                            >  2:3897568/1‑150 (MQ=255)

                           gagaAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAAGTGGTCGGc                                                                                                                            >  2:3897731/1‑150 (MQ=255)

                             gaAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGa                                                                                                                          >  2:4110615/1‑150 (MQ=255)

                              aaaCGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGaa                                                                                                                         <  2:528389/150‑1 (MQ=255)

                              aaaCGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGaa                                                                                                                         >  1:1482490/1‑150 (MQ=255)

                               aaCGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAg                                                                                                                        <  2:4036228/150‑1 (MQ=255)

                                    aaTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGAtttt                                                                                                                   <  2:3599628/150‑1 (MQ=255)

                                     aTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTg                                                                                                                  <  1:4832023/150‑1 (MQ=255)

                                     aTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTg                                                                                                                  <  1:1458992/150‑1 (MQ=255)

                                     aTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTg                                                                                                                  <  1:277626/150‑1 (MQ=255)

                                       ggtggCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGt                                                                                                                <  1:4632040/150‑1 (MQ=255)

                                             ggcgATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGc                                                                                                          >  1:4580273/1‑150 (MQ=255)

                                                gATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGc                                                                                                       >  2:2352369/1‑150 (MQ=255)

                                                       tCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGccc                                                                                                >  2:4842649/1‑150 (MQ=255)

                                                       tCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGccc                                                                                                >  2:5083495/1‑150 (MQ=255)

                                                       tCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGccc                                                                                                >  2:4335471/1‑150 (MQ=255)

                                                       tCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGccc                                                                                                >  2:4003052/1‑150 (MQ=255)

                                                       tCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGccc                                                                                                >  1:723159/1‑150 (MQ=255)

                                                           aGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGc                                                                                            <  1:1695336/150‑1 (MQ=255)

                                                             gTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCgg                                                                                          <  2:1289290/150‑1 (MQ=255)

                                                             gTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCgg                                                                                          <  1:620361/150‑1 (MQ=255)

                                                             gTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCgg                                                                                          >  1:95606/1‑150 (MQ=255)

                                                                ccATAGCGTATGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCAGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAac                                                                                       >  2:5000213/1‑150 (MQ=255)

                                                                   tAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACt                                                                                    >  2:3529910/1‑150 (MQ=255)

                                                                    aGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTg                                                                                   >  2:4674006/1‑150 (MQ=255)

                                                                    aGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTg                                                                                   <  2:1033927/150‑1 (MQ=255)

                                                                      cGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATGCTCTCGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCa                                                                                 <  2:1751565/150‑1 (MQ=255)

                                                                       gTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAg                                                                                <  2:3686585/150‑1 (MQ=255)

                                                                        tttGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGa                                                                               <  1:3853730/150‑1 (MQ=255)

                                                                         tgcataggCACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGaa                                                                              >  2:4585561/7‑149 (MQ=255)

                                                                           gAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGt                                                                            <  1:3227938/150‑1 (MQ=255)

                                                                              cGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCgg                                                                         <  2:250025/150‑1 (MQ=255)

                                                                               ggCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCggg                                                                        <  1:3364492/150‑1 (MQ=255)

                                                                                gCACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGTCGGGa                                                                       >  2:1596471/1‑150 (MQ=255)

                                                                                gCACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGTCGGGa                                                                       <  2:2597248/150‑1 (MQ=255)

                                                                                 cACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGTCGGGaa                                                                      <  2:638621/150‑1 (MQ=255)

                                                                                  aCGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAAc                                                                     >  1:2919745/1‑150 (MQ=255)

                                                                                  aCGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAAc                                                                     >  1:2684939/1‑150 (MQ=255)

                                                                                  aCGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAAc                                                                     >  1:133264/1‑150 (MQ=255)

                                                                                  aCGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGTCGGGAAc                                                                     >  1:3686608/1‑150 (MQ=255)

                                                                                   cGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACa                                                                    <  2:2101824/150‑1 (MQ=255)

                                                                                    gCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGTCGGGAACat                                                                   >  1:4169167/1‑149 (MQ=255)

                                                                                     cTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGTCGGGAACatt                                                                  <  1:1692348/150‑3 (MQ=255)

                                                                                     cTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGTCGGGAACatt                                                                  <  2:2908762/150‑3 (MQ=255)

                                                                                            ggcgCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTgg                                                           <  1:511687/150‑1 (MQ=255)

                                                                                                cTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTc                                                       <  1:2591092/150‑1 (MQ=255)

                                                                                                   ttCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGCCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGaa                                                    >  2:3294816/1‑150 (MQ=255)

                                                                                                        tggctggcAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGccc                                               <  1:1639756/150‑1 (MQ=255)

                                                                                                         ggctggcAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCa                                              <  2:1865054/150‑1 (MQ=255)

                                                                                                               cAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGATCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGt                                        <  1:4972170/150‑1 (MQ=255)

                                                                                                               cAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGt                                        <  1:2432074/150‑1 (MQ=255)

                                                                                                               cAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGt                                        >  2:1662673/1‑150 (MQ=255)

                                                                                                               cAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGt                                        <  1:3093242/150‑1 (MQ=255)

                                                                                                                aGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTg                                       >  1:3614662/1‑150 (MQ=255)

                                                                                                                aGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTg                                       <  2:1031093/150‑1 (MQ=255)

                                                                                                                 ggCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTgg                                      <  1:4678302/150‑1 (MQ=255)

                                                                                                                 ggCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTgg                                      <  2:312298/150‑1 (MQ=255)

                                                                                                                  gCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGa                                     <  2:931152/150‑1 (MQ=255)

                                                                                                                    tGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGaaa                                   >  2:2725771/1‑150 (MQ=255)

                                                                                                                       cGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGt                                >  1:1606695/1‑150 (MQ=255)

                                                                                                                        gATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACCGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGtt                               <  2:4982919/150‑1 (MQ=255)

                                                                                                                        gATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGtt                               >  1:3931101/1‑150 (MQ=255)

                                                                                                                         aTCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTtg                              <  1:2923517/150‑1 (MQ=255)

                                                                                                                         aTCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTtg                              <  2:4220052/150‑1 (MQ=255)

                                                                                                                          tCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTtga                             <  1:173085/150‑1 (MQ=255)

                                                                                                                           ccAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTtgat                            <  2:3139527/150‑1 (MQ=255)

                                                                                                                           ccAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTtgat                            <  2:1162606/150‑1 (MQ=255)

                                                                                                                               ccGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATc                        <  1:4952677/150‑1 (MQ=255)

                                                                                                                                 ggggAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCAc                      <  1:2228213/150‑1 (MQ=255)

                                                                                                                                      ggaCGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGcccc                 <  1:319147/150‑1 (MQ=255)

                                                                                                                                            tgttgATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCt           <  2:324407/150‑1 (MQ=255)

                                                                                                                                             gttgATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTg          <  1:3106524/150‑1 (MQ=255)

                                                                                                                                              ttgATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTgg         <  2:28492/150‑1 (MQ=255)

                                                                                                                                              ttgATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTgg         <  1:4888958/150‑1 (MQ=255)

                                                                                                                                               tgATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGgc        <  1:2446430/150‑1 (MQ=255)

                                                                                                                                                gATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGgcg       >  1:668486/1‑150 (MQ=255)

                                                                                                                                                    cTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGACCAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGc   >  2:886312/1‑150 (MQ=255)

                                                                                                                                                    cTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGc   >  1:4041459/1‑150 (MQ=255)

                                                                                                                                                     tCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCa  <  1:4396896/150‑1 (MQ=255)

                                                                                                                                                     tCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCa  >  1:3034870/1‑150 (MQ=255)

                                                                                                                                                     tCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCa  >  2:527296/1‑150 (MQ=255)

                                                                                                                                                     tCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCa  >  1:50487/1‑150 (MQ=255)

GGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCA  >  NC_002947/2482652‑2482950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`