BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,482,821	T→G	5.4%	Q164P (CAA→CCA) ‡	PP_2175 ←	3‑oxoacyl‑ACP reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,482,821	0	T	G	5.4%	689.6 / 20.6	202	Q164P (CAA→CCA) ‡	PP_2175	3‑oxoacyl‑ACP reductase
Reads supporting (aligned to +/- strand): ref base T (73/118); new base G (7/4); total (80/122)
Fisher's exact test for biased strand distribution p-value = 1.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

Only 100 of 205 total aligned reads displayed.

GCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGA  >  NC_002947/2482673‑2482970

gCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTg                                                                                                                                                      <  1:4369594/150‑1 (MQ=255)

gCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGAGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTg                                                                                                                                                      <  1:1043367/150‑1 (MQ=255)

   ggCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCTCGTTGTCCCCGGCGAATTGGTc                                                                                                                                                   <  1:1487702/150‑1 (MQ=255)

   ggCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTc                                                                                                                                                   <  2:4248926/150‑1 (MQ=255)

   ggCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTc                                                                                                                                                   >  2:3732886/1‑150 (MQ=255)

    gCGAGAACCGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCg                                                                                                                                                  >  2:4297106/1‑150 (MQ=255)

    gCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCg                                                                                                                                                  <  1:3846295/150‑1 (MQ=255)

    gCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTATCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCg                                                                                                                                                  <  2:4996955/150‑1 (MQ=255)

      gagaAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAAGTGGTCGGc                                                                                                                                                >  2:3897731/1‑150 (MQ=255)

       agaAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCg                                                                                                                                               >  2:4119328/1‑150 (MQ=255)

          aaCGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAg                                                                                                                                            <  2:4036228/150‑1 (MQ=255)

            cGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGAt                                                                                                                                          >  1:570123/1‑150 (MQ=255)

                aTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTg                                                                                                                                      <  1:1729849/150‑1 (MQ=255)

                aTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTg                                                                                                                                      <  1:277626/150‑1 (MQ=255)

                aTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTg                                                                                                                                      <  1:50015/150‑1 (MQ=255)

                  ggtggCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGt                                                                                                                                    <  2:4964939/150‑1 (MQ=255)

                  ggtggCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGt                                                                                                                                    <  1:4632040/150‑1 (MQ=255)

                        ggcgATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGc                                                                                                                              >  2:5052112/1‑150 (MQ=255)

                           gATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGc                                                                                                                           >  2:2352369/1‑150 (MQ=255)

                             tttCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCa                                                                                                                         >  1:4220052/1‑150 (MQ=255)

                              ttCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAg                                                                                                                        <  2:3411016/150‑1 (MQ=255)

                                  tCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGccc                                                                                                                    >  2:4335471/1‑150 (MQ=255)

                                  tCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGccc                                                                                                                    >  2:4003052/1‑150 (MQ=255)

                                  tCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGccc                                                                                                                    >  2:4842649/1‑150 (MQ=255)

                                  tCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGccc                                                                                                                    >  1:723032/1‑150 (MQ=255)

                                    gCAGGTGCCATAGCGTTTGAGCGGCTCGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGTGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGa                                                                                                                  <  1:2704822/150‑1 (MQ=255)

                                     cAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAg                                                                                                                 <  1:1579740/150‑1 (MQ=255)

                                      aGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGc                                                                                                                <  1:1695291/150‑1 (MQ=255)

                                        gTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCgg                                                                                                              <  1:620361/150‑1 (MQ=255)

                                         tGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGa                                                                                                             >  2:2007967/1‑150 (MQ=255)

                                             aTAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGACTTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAacac                                                                                                         >  1:4550592/1‑150 (MQ=255)

                                             aTAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAacac                                                                                                         >  2:3132642/1‑150 (MQ=255)

                                               aGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTg                                                                                                       >  2:4674006/1‑150 (MQ=255)

                                               aGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTg                                                                                                       <  2:517889/150‑1 (MQ=255)

                                                 cGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATGCTCTCGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCa                                                                                                     <  2:1751565/150‑1 (MQ=255)

                                                   tttGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGa                                                                                                   <  1:3853730/150‑1 (MQ=255)

                                                   tttGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGa                                                                                                   <  1:161630/150‑1 (MQ=255)

                                                    tgcataggCACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGaa                                                                                                  >  1:1680708/7‑149 (MQ=255)

                                                      gAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGt                                                                                                <  1:3227938/150‑1 (MQ=255)

                                                      cataggCACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGt                                                                                                >  1:4394952/5‑150 (MQ=255)

                                                         cGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCgg                                                                                             <  2:250025/150‑1 (MQ=255)

                                                          ggCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCggg                                                                                            <  1:3364492/150‑1 (MQ=255)

                                                           gCACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGTCGGGa                                                                                           >  2:1596471/1‑150 (MQ=255)

                                                           gCACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGTCGGGa                                                                                           <  2:2597248/150‑1 (MQ=255)

                                                            cACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGaa                                                                                          <  1:1471956/150‑1 (MQ=255)

                                                            cACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGTCGGGaa                                                                                          <  2:638621/150‑1 (MQ=255)

                                                             aCGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAAc                                                                                         >  1:2919745/1‑150 (MQ=255)

                                                             aCGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAAc                                                                                         >  1:133264/1‑150 (MQ=255)

                                                             aCGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGTCGGGAAc                                                                                         >  1:4917493/1‑150 (MQ=255)

                                                                     gcggcgCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACt                                                                                 <  1:3694687/150‑1 (MQ=255)

                                                                           cTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTc                                                                           <  1:2591092/150‑1 (MQ=255)

                                                                             gTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGa                                                                         <  2:383889/150‑1 (MQ=255)

                                                                                    ggctggcAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCa                                                                  <  2:3567125/150‑1 (MQ=255)

                                                                                    ggctggcAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCa                                                                  <  2:1865054/150‑1 (MQ=255)

                                                                                        ggcAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCg                                                              <  2:2875376/150‑1 (MQ=255)

                                                                                          cAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGt                                                            <  1:3093242/150‑1 (MQ=255)

                                                                                           aGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTg                                                           >  1:5090163/1‑150 (MQ=255)

                                                                                           aGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTg                                                           >  1:3614662/1‑150 (MQ=255)

                                                                                            ggCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTgg                                                          >  1:4971006/1‑150 (MQ=255)

                                                                                            ggCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTgg                                                          <  1:4678302/150‑1 (MQ=255)

                                                                                             gCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGa                                                         <  2:931152/150‑1 (MQ=255)

                                                                                               tGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGaaa                                                       <  1:1976784/150‑1 (MQ=255)

                                                                                               tGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGaaa                                                       >  2:2725771/1‑150 (MQ=255)

                                                                                                gTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAAt                                                      >  1:960975/1‑150 (MQ=255)

                                                                                                  cGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGt                                                    <  2:4580273/150‑1 (MQ=255)

                                                                                                    aTCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTtg                                                  <  1:4861598/150‑1 (MQ=255)

                                                                                                     tCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTtga                                                 <  1:473888/150‑1 (MQ=255)

                                                                                                     tCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTtga                                                 <  2:103127/150‑1 (MQ=255)

                                                                                                      ccAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTtgat                                                <  1:744209/150‑1 (MQ=255)

                                                                                                          ccGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATc                                            <  2:4591907/150‑1 (MQ=255)

                                                                                                           cGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCTGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCa                                           <  2:3608492/150‑1 (MQ=255)

                                                                                                            ggggAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCAc                                          <  1:2228213/150‑1 (MQ=255)

                                                                                                               gaggaCGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGcc                                       >  1:934765/1‑150 (MQ=255)

                                                                                                                 ggaCGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGcccc                                     <  1:319147/150‑1 (MQ=255)

                                                                                                                  gaCGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGccccc                                    >  2:1213230/1‑150 (MQ=255)

                                                                                                                       tgttgATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCt                               <  2:324749/150‑1 (MQ=255)

                                                                                                                        gttgATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTg                              <  1:3106524/150‑1 (MQ=255)

                                                                                                                        gttgATCCTCACGTTGTCCCCGGCGAACTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGACGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTg                              >  2:3235030/1‑150 (MQ=255)

                                                                                                                         ttgATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTgg                             <  2:28492/150‑1 (MQ=255)

                                                                                                                          tgATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGgc                            <  1:2446430/150‑1 (MQ=255)

                                                                                                                             tCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCt                         <  2:1160194/150‑1 (MQ=255)

                                                                                                                               cTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGACCAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGc                       >  2:886312/1‑150 (MQ=255)

                                                                                                                               cTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGc                       >  1:4041459/1‑150 (MQ=255)

                                                                                                                                tCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCa                      >  1:3034870/1‑150 (MQ=255)

                                                                                                                                tCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCa                      >  2:527369/1‑150 (MQ=255)

                                                                                                                                     ttGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAg                 <  1:2352369/150‑1 (MQ=255)

                                                                                                                                      tGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAgg                <  1:413373/150‑1 (MQ=255)

                                                                                                                                        tCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGc              <  2:1044687/150‑1 (MQ=255)

                                                                                                                                        tCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGc              >  2:211544/1‑150 (MQ=255)

                                                                                                                                        tCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTAGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGc              >  2:529277/1‑150 (MQ=255)

                                                                                                                                         ccccGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCg             >  2:2926120/1‑150 (MQ=255)

                                                                                                                                          cccGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGt            <  1:367355/150‑1 (MQ=255)

                                                                                                                                            cGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCa          >  1:399909/1‑150 (MQ=255)

                                                                                                                                             ggCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCAc         <  1:1370619/150‑1 (MQ=255)

                                                                                                                                             ggCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCAc         >  2:1675370/1‑150 (MQ=255)

                                                                                                                                              gCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCAcc        <  2:1450001/150‑1 (MQ=255)

                                                                                                                                               cGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCa       <  1:3897568/150‑1 (MQ=255)

                                                                                                                                               cGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCa       <  1:3897731/150‑1 (MQ=255)

                                                                                                                                                  aTTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGc    <  1:108624/150‑1 (MQ=255)

                                                                                                                                                   ttGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCg   <  1:2660689/150‑1 (MQ=255)

GCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGA  >  NC_002947/2482673‑2482970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`