BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,482,856	C→T	5.9%	S152S (TCG→TCA) ‡	PP_2175 ←	3‑oxoacyl‑ACP reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,482,856	0	C	T	5.9%	579.0 / 16.9	186	S152S (TCG→TCA) ‡	PP_2175	3‑oxoacyl‑ACP reductase
Reads supporting (aligned to +/- strand): ref base C (59/116); new base T (7/4); total (66/120)
Fisher's exact test for biased strand distribution p-value = 5.54e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 196 total aligned reads displayed.

TCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGAATGACATTGAGCAGATAGGTGTCCA  >  NC_002947/2482707‑2483004

tCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGccc                                                                                                                                                      >  2:4003052/1‑150 (MQ=255)

tCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGccc                                                                                                                                                      >  1:723159/1‑150 (MQ=255)

  gCAGGTGCCATAGCGTTTGAGCGGCTCGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGTGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGa                                                                                                                                                    <  1:2704822/150‑1 (MQ=255)

    aGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGc                                                                                                                                                  <  1:1695336/150‑1 (MQ=255)

      gTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCgg                                                                                                                                                >  1:95606/1‑150 (MQ=255)

         ccATAGCGTATGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCAGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAac                                                                                                                                             >  2:5000213/1‑150 (MQ=255)

           aTAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGACTTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAacac                                                                                                                                           >  1:4550592/1‑150 (MQ=255)

           aTAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAacac                                                                                                                                           >  2:3132642/1‑150 (MQ=255)

            tAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACt                                                                                                                                          >  2:3529910/1‑150 (MQ=255)

             aGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTg                                                                                                                                         <  2:1033927/150‑1 (MQ=255)

             aGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTg                                                                                                                                         <  2:517889/150‑1 (MQ=255)

             aGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTg                                                                                                                                         >  2:4674006/1‑150 (MQ=255)

               cGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATGCTCTCGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCa                                                                                                                                       <  2:1751565/150‑1 (MQ=255)

                 tttGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGa                                                                                                                                     <  1:3853730/150‑1 (MQ=255)

                  tgcataggCACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGaa                                                                                                                                    >  1:1680708/7‑149 (MQ=255)

                  tgcataggCACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGaa                                                                                                                                    >  2:4585561/7‑149 (MQ=255)

                    gAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGt                                                                                                                                  <  1:3227938/150‑1 (MQ=255)

                    cataggCACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGt                                                                                                                                  >  1:4394952/5‑150 (MQ=255)

                        ggCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCggg                                                                                                                              <  1:3364492/150‑1 (MQ=255)

                           aCGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAAc                                                                                                                           >  1:2684939/1‑150 (MQ=255)

                           aCGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAAc                                                                                                                           >  1:133264/1‑150 (MQ=255)

                            cGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACa                                                                                                                          >  1:2922597/1‑150 (MQ=255)

                            cGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACa                                                                                                                          <  2:2101824/150‑1 (MQ=255)

                              cTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGTCGGGAACatt                                                                                                                        <  1:1692348/150‑3 (MQ=255)

                                   gcggcgCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACt                                                                                                                   >  2:1598262/1‑150 (MQ=255)

                                   gcggcgCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACt                                                                                                                   <  1:3694687/150‑1 (MQ=255)

                                   gcggcgCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACt                                                                                                                   >  2:894433/1‑150 (MQ=255)

                                     ggcgCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTgg                                                                                                                 <  1:511687/150‑1 (MQ=255)

                                         cTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTc                                                                                                             <  1:2591092/150‑1 (MQ=255)

                                                  ggctggcAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCa                                                                                                    <  2:1865054/150‑1 (MQ=255)

                                                      ggcAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCg                                                                                                <  2:2875376/150‑1 (MQ=255)

                                                        cAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGATCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGt                                                                                              <  1:4972170/150‑1 (MQ=255)

                                                        cAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGt                                                                                              <  1:3093271/150‑1 (MQ=255)

                                                        cAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGt                                                                                              >  2:1662673/1‑150 (MQ=255)

                                                        cAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGt                                                                                              <  1:2432074/150‑1 (MQ=255)

                                                        cAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGt                                                                                              <  1:231480/150‑1 (MQ=255)

                                                         aGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTg                                                                                             <  2:1031093/150‑1 (MQ=255)

                                                           gCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGa                                                                                           <  2:931231/150‑1 (MQ=255)

                                                             tGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGaaa                                                                                         >  2:2725771/1‑150 (MQ=255)

                                                             tGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGaaa                                                                                         <  1:1976784/150‑1 (MQ=255)

                                                                cGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGt                                                                                      >  1:1606695/1‑150 (MQ=255)

                                                                   tCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGTACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTtga                                                                                   <  2:1574693/150‑1 (MQ=255)

                                                                   tCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTtga                                                                                   <  1:4596337/150‑1 (MQ=255)

                                                                    ccAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTtgat                                                                                  <  1:3767777/150‑1 (MQ=255)

                                                                        ccGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATc                                                                              <  1:4952677/150‑1 (MQ=255)

                                                                         cGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCTGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCa                                                                             <  2:3608492/150‑1 (MQ=255)

                                                                             gaggaCGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGcc                                                                         >  1:934765/1‑150 (MQ=255)

                                                                               ggaCGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGcccc                                                                       <  1:319147/150‑1 (MQ=255)

                                                                                gaCGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGccccc                                                                      >  1:2811587/1‑150 (MQ=255)

                                                                                gaCGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGccccc                                                                      >  2:1213230/1‑150 (MQ=255)

                                                                                    ttgtagATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTc                                                                  <  2:2584083/150‑1 (MQ=255)

                                                                                     tgttgATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCt                                                                 <  2:324749/150‑1 (MQ=255)

                                                                                      gttgATCCTCACGTTGTCCCCGGCGAACTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGACGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTg                                                                >  2:3235030/1‑150 (MQ=255)

                                                                                       ttgATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTgg                                                               <  1:4888958/150‑1 (MQ=255)

                                                                                       ttgATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTgg                                                               <  2:28492/150‑1 (MQ=255)

                                                                                         gATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGgcg                                                             >  1:668486/1‑150 (MQ=255)

                                                                                           tCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCt                                                           >  2:2132375/1‑150 (MQ=255)

                                                                                           tCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCt                                                           <  2:1160194/150‑1 (MQ=255)

                                                                                             cTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGc                                                         >  1:4041459/1‑150 (MQ=255)

                                                                                              tCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCa                                                        >  1:3034870/1‑150 (MQ=255)

                                                                                              tCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCa                                                        >  2:527369/1‑150 (MQ=255)

                                                                                                 cGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGt                                                     <  2:494521/150‑1 (MQ=255)

                                                                                                    tGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAgg                                                  <  1:413373/150‑1 (MQ=255)

                                                                                                       ccccGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCg                                               >  2:2926120/1‑150 (MQ=255)

                                                                                                        cccGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGt                                              <  1:367355/150‑1 (MQ=255)

                                                                                                           ggCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCAc                                           <  1:1370619/150‑1 (MQ=255)

                                                                                                            gCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCAcc                                          <  2:1450001/150‑1 (MQ=255)

                                                                                                             cGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCa                                         <  1:3897568/150‑1 (MQ=255)

                                                                                                             cGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCa                                         <  1:3897731/150‑1 (MQ=255)

                                                                                                             cGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCa                                         <  1:4694688/150‑1 (MQ=255)

                                                                                                                aTTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGc                                      <  1:579635/150‑1 (MQ=255)

                                                                                                                aTTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGc                                      <  1:108624/150‑1 (MQ=255)

                                                                                                                  tGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGa                                    <  2:4253109/150‑1 (MQ=255)

                                                                                                                     tCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGc                                      <  1:759617/145‑1 (MQ=255)

                                                                                                                     tCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGc                                      >  2:759617/1‑145 (MQ=255)

                                                                                                                     tCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTc                                 >  2:2947337/1‑150 (MQ=255)

                                                                                                                       ggCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCgg                               <  1:4790721/150‑1 (MQ=255)

                                                                                                                        gCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCggg                              <  1:3538604/150‑1 (MQ=255)

                                                                                                                         cGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGc                             >  1:1040641/1‑150 (MQ=255)

                                                                                                                          gAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCg                            >  2:2498106/1‑150 (MQ=255)

                                                                                                                          gAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCg                            >  1:1621759/1‑150 (MQ=255)

                                                                                                                          gAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCg                            >  1:3108177/1‑150 (MQ=255)

                                                                                                                           aaGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGa                           >  2:3744341/1‑150 (MQ=255)

                                                                                                                           aaGATTTTGGTGAATGCGGCCAGACCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGa                           >  2:2290065/1‑150 (MQ=255)

                                                                                                                             gATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGAAt                         <  2:3852568/150‑1 (MQ=255)

                                                                                                                             gATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGAAt                         <  1:4297106/150‑1 (MQ=255)

                                                                                                                                 ttGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGAATGACa                     <  1:1176212/150‑1 (MQ=255)

                                                                                                                                   ggTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGAATGACAtt                   <  1:4003052/150‑1 (MQ=255)

                                                                                                                                   ggTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGAATGACAtt                   <  1:3944371/150‑1 (MQ=255)

                                                                                                                                     tGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGAATGACATTGa                 <  2:570123/150‑1 (MQ=255)

                                                                                                                                      gAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGAATGACATTGAg                <  2:4550592/150‑1 (MQ=255)

                                                                                                                                       aaTGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGAATGACATTGAGc               <  1:4674006/150‑1 (MQ=255)

                                                                                                                                            ggCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGAATGACATTGAGCAGATa          <  1:4532612/150‑1 (MQ=255)

                                                                                                                                             gCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGAATGACATTGAGCAGATAg         <  1:5083495/150‑1 (MQ=255)

                                                                                                                                             gCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGAATGACATTGAGCAGATAg         >  1:1505635/1‑150 (MQ=255)

                                                                                                                                             gCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGAATGACATTGAGCAGATAg         >  2:4195923/1‑150 (MQ=255)

                                                                                                                                              ccAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGAATGACATTGAGCAGATAgg        >  1:2066322/1‑150 (MQ=255)

                                                                                                                                               cAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGAATGACATTGAGCAGATAGgt       <  2:3318380/150‑1 (MQ=255)

                                                                                                                                                    ccGAGCGGAACACTGCAGACGTCGGGACCAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGAATGACATTGAGCAGATAGGTGTCCa  >  2:744647/1‑150 (MQ=255)

                                                                                                                                                    ccGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGAATGACATTGAGCAGATAGGTGTCCa  >  1:3466909/1‑150 (MQ=255)

TCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCCCCCTTTCTGGCGCTGCATGTAGGGCGTCACCAGGCGAGTCGGGCGAATGACATTGAGCAGATAGGTGTCCA  >  NC_002947/2482707‑2483004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`