BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,637,546	C→T	5.4%	L331L (CTG→CTA) ‡	PP_2305 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,637,546	0	C	T	5.4%	632.7 / 15.9	201	L331L (CTG→CTA) ‡	PP_2305	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (90/100); new base T (6/5); total (96/105)
Fisher's exact test for biased strand distribution p-value = 7.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

AGGCCGCCTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGATCAAATCCA  >  NC_002947/2637398‑2637694

aGGCCGCCTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCa                                                                                                                                                     >  2:90975/1‑150 (MQ=255)

aGGCCGCCTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCa                                                                                                                                                     >  2:2172494/1‑150 (MQ=255)

  gccgccTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAgg                                                                                                                                                   >  1:2461953/1‑150 (MQ=255)

   ccgccTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAggg                                                                                                                                                  >  1:2248670/1‑150 (MQ=255)

      ccTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGa                                                                                                                                               <  2:4758660/150‑1 (MQ=255)

       cTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCTGGGGCGag                                                                                                                                              <  2:2052804/150‑2 (MQ=255)

       cTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGAt                                                                                                                                              <  1:5009484/150‑1 (MQ=255)

       cTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGAt                                                                                                                                              >  1:513387/1‑150 (MQ=255)

        tCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCTGGGGCGaga                                                                                                                                             <  2:2652819/150‑3 (MQ=255)

        tCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATg                                                                                                                                             <  2:4055811/150‑1 (MQ=255)

                 cTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGgc                                                                                                                                    >  1:1783273/1‑150 (MQ=255)

                  tGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGgcg                                                                                                                                   >  2:2573727/1‑150 (MQ=255)

                    tttCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGcc                                                                                                                                 >  2:1251855/1‑150 (MQ=255)

                      tCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCtt                                                                                                                               >  1:1302201/1‑150 (MQ=255)

                         ttttCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTa                                                                                                                            <  2:3758424/150‑1 (MQ=255)

                         ttttCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTa                                                                                                                            >  1:486435/1‑150 (MQ=255)

                         ttttCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTa                                                                                                                            <  1:369499/150‑1 (MQ=255)

                           ttCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGa                                                                                                                          >  1:3808624/1‑150 (MQ=255)

                            tCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGaa                                                                                                                         <  2:3589617/150‑1 (MQ=255)

                              gCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATACCTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACt                                                                                                                       <  2:3852841/150‑1 (MQ=255)

                                cAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCg                                                                                                                     >  1:1194612/1‑150 (MQ=255)

                                cAGCCGCGAGCACGGTCATGGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATACCTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCg                                                                                                                     >  2:4675230/1‑150 (MQ=255)

                                      cgAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAAc                                                                                                               <  2:645655/150‑1 (MQ=255)

                                       gAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAAcc                                                                                                              <  2:2527800/150‑1 (MQ=255)

                                        aGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCg                                                                                                             <  1:1386662/150‑1 (MQ=255)

                                             ggTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGcaca                                                                                                        >  2:5118123/1‑150 (MQ=255)

                                             ggTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGcaca                                                                                                        >  1:4646293/1‑150 (MQ=255)

                                                    gTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCtt                                                                                                 <  2:2964834/150‑1 (MQ=255)

                                                    gTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCtt                                                                                                 <  1:2076737/150‑1 (MQ=255)

                                                     tCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTg                                                                                                <  2:181311/150‑1 (MQ=255)

                                                             cacaGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGtt                                                                                        <  2:1941649/150‑1 (MQ=255)

                                                                 gTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATc                                                                                    >  2:877816/1‑150 (MQ=255)

                                                                 gTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATc                                                                                    >  2:4685057/1‑150 (MQ=255)

                                                                  tGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCa                                                                                   <  1:4848745/150‑1 (MQ=255)

                                                                   gCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAg                                                                                  >  1:4371317/1‑150 (MQ=255)

                                                                    ccTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGc                                                                                 <  2:4431856/150‑1 (MQ=255)

                                                                     cTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGca                                                                                >  1:4330682/1‑150 (MQ=255)

                                                                       tCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGca                                                                                >  1:760284/1‑148 (MQ=255)

                                                                            ggTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCgg                                                                         <  1:567355/150‑1 (MQ=255)

                                                                             gTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGt                                                                        >  2:3310026/1‑150 (MQ=255)

                                                                              tGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTg                                                                       <  1:2832068/150‑1 (MQ=255)

                                                                                  gcTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTGGGGTACGTTGATCAGCACATCCGGTGGATa                                                                   >  1:4828011/1‑150 (MQ=255)

                                                                                  gcTAACCAGGTGCTACCGCTTCACCCCTTCATAACCGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATa                                                                   >  2:810496/1‑150 (MQ=255)

                                                                                     aaCCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGcc                                                                >  1:1540687/1‑150 (MQ=255)

                                                                                      aCCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCg                                                               >  2:4319224/1‑150 (MQ=255)

                                                                                      aCCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCg                                                               <  1:4413952/150‑1 (MQ=255)

                                                                                        cAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCTGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCAGGTGTATAGCCGGc                                                             >  1:2089376/1‑150 (MQ=255)

                                                                                              cTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCtt                                                       <  1:220318/150‑1 (MQ=255)

                                                                                               tACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGCAAAACCGGCACACCCGCTTGGGTACGTTGAGCAGCACATCCGGTGGATAGCCGGCTATCTTg                                                      >  2:2906298/1‑150 (MQ=255)

                                                                                               tACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTg                                                      >  2:3692766/1‑150 (MQ=255)

                                                                                               tACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTg                                                      >  2:669318/1‑150 (MQ=255)

                                                                                               tACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTg                                                      >  2:4592371/1‑150 (MQ=255)

                                                                                               tACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTg                                                      <  2:1783273/150‑1 (MQ=255)

                                                                                                 ccGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCTCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTa                                                    <  2:596776/150‑1 (MQ=255)

                                                                                                 ccGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTa                                                    <  1:921640/150‑1 (MQ=255)

                                                                                                 ccGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTa                                                    <  1:2407815/150‑1 (MQ=255)

                                                                                                     ttCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACtgt                                                >  2:273599/1‑150 (MQ=255)

                                                                                                     ttCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGCCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACtgt                                                <  2:4866353/150‑1 (MQ=255)

                                                                                                        aCCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGcc                                             >  2:1213532/1‑150 (MQ=255)

                                                                                                          cccTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCaa                                           >  2:1142960/1‑150 (MQ=255)

                                                                                                          cccTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCaa                                           >  2:4183638/1‑150 (MQ=255)

                                                                                                          cccTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCaa                                           >  1:4476998/1‑150 (MQ=255)

                                                                                                          cccTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCaa                                           <  1:4711795/150‑1 (MQ=255)

                                                                                                           ccTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAAc                                          <  2:1633898/150‑1 (MQ=255)

                                                                                                           ccTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAAc                                          <  1:3888803/150‑1 (MQ=255)

                                                                                                             ttCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGa                                        >  2:4306941/1‑150 (MQ=255)

                                                                                                              tCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGaa                                       <  2:2135711/150‑1 (MQ=255)

                                                                                                              tCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGaa                                       <  1:1547555/150‑1 (MQ=255)

                                                                                                                aTAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGt                                     <  2:2461953/150‑1 (MQ=255)

                                                                                                                  aaCTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCt                                   >  1:1727441/1‑150 (MQ=255)

                                                                                                                    cTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGc                                 <  2:401388/150‑1 (MQ=255)

                                                                                                                     tGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCa                                <  1:4023479/150‑1 (MQ=255)

                                                                                                                     tGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCa                                <  1:2713797/150‑1 (MQ=255)

                                                                                                                       tCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATg                              >  2:1628061/1‑150 (MQ=255)

                                                                                                                       tCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGAACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATg                              >  2:1112596/1‑150 (MQ=255)

                                                                                                                             gTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCg                        >  2:2320482/1‑150 (MQ=255)

                                                                                                                               aTCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGaa                      <  2:3540016/150‑1 (MQ=255)

                                                                                                                               aTCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGaa                      <  2:1370844/150‑1 (MQ=255)

                                                                                                                                    gTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGctct                 <  1:1955274/150‑1 (MQ=255)

                                                                                                                                      gCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCTACGAAGTCTGCATGACCTCGAAGCTCTgg               <  2:2406411/150‑1 (MQ=255)

                                                                                                                                       cAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGt              <  1:2573727/150‑1 (MQ=255)

                                                                                                                                        aaTCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTgg               >  1:1485144/1‑148 (MQ=255)

                                                                                                                                        aaTCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTgg               <  2:1485144/148‑1 (MQ=255)

                                                                                                                                        aaTCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGtt             >  1:3120711/1‑150 (MQ=255)

                                                                                                                                        aaTCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGtt             <  2:3824960/150‑1 (MQ=255)

                                                                                                                                        aaTCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGtt             >  2:482132/1‑150 (MQ=255)

                                                                                                                                         aTCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTg            <  1:1251855/150‑1 (MQ=255)

                                                                                                                                           tcatcGCCCTGGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGAt          >  2:3442233/4‑150 (MQ=255)

                                                                                                                                           tcatcGCCCTGGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGAt          >  2:185999/4‑150 (MQ=255)

                                                                                                                                           tcatcGCCCTGGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGAt          >  2:3076499/4‑150 (MQ=255)

                                                                                                                                           ctctcGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGAt          <  2:3177415/150‑1 (MQ=255)

                                                                                                                                            tctcGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGATc         >  2:2917679/1‑150 (MQ=255)

                                                                                                                                            tctcGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGATc         >  1:706305/1‑150 (MQ=255)

                                                                                                                                              tcGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGATCaa       >  1:202140/1‑150 (MQ=255)

                                                                                                                                               cGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGATCaaa      <  2:1302201/150‑1 (MQ=255)

                                                                                                                                               cGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGATCaaa      <  2:4995851/150‑1 (MQ=255)

                                                                                                                                                  cccAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGATCAAATcc   >  1:1043747/1‑150 (MQ=255)

                                                                                                                                                  cccAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGATCAAATcc   <  1:2979378/150‑1 (MQ=255)

                                                                                                                                                   ccAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTg                <  1:1626283/136‑1 (MQ=255)

                                                                                                                                                   ccAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTg                >  2:1626283/1‑136 (MQ=255)

AGGCCGCCTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGATCAAATCCA  >  NC_002947/2637398‑2637694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`