BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,637,547	A→G	5.4%	L331P (CTG→CCG) ‡	PP_2305 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,637,547	0	A	G	5.4%	644.6 / 15.6	203	L331P (CTG→CCG) ‡	PP_2305	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (89/102); new base G (6/5); total (95/108)
Fisher's exact test for biased strand distribution p-value = 7.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

Only 100 of 208 total aligned reads displayed.

AGGCCGCCTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGATCAAATCCAGC  >  NC_002947/2637398‑2637696

aGGCCGCCTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCa                                                                                                                                                       >  2:90975/1‑150 (MQ=255)

aGGCCGCCTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCa                                                                                                                                                       >  2:2172494/1‑150 (MQ=255)

 ggCCGCCTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAg                                                                                                                                                      >  1:2152435/1‑150 (MQ=255)

  gccgccTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAgg                                                                                                                                                     >  1:2461953/1‑150 (MQ=255)

   ccgccTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAggg                                                                                                                                                    >  1:2248670/1‑150 (MQ=255)

      ccTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGa                                                                                                                                                 <  2:4758660/150‑1 (MQ=255)

       cTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGAt                                                                                                                                                <  1:5009484/150‑1 (MQ=255)

       cTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGAt                                                                                                                                                <  1:4164640/150‑1 (MQ=255)

        tCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCTGGGGCGaga                                                                                                                                               <  2:2958328/150‑3 (MQ=255)

        tCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCTGGGGCGaga                                                                                                                                               <  1:2085702/150‑3 (MQ=255)

         cGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGa                                                                                                                                              <  1:604228/150‑1 (MQ=255)

                 cTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGgc                                                                                                                                      >  1:1783273/1‑150 (MQ=255)

                    tttCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGcc                                                                                                                                   >  2:1251855/1‑150 (MQ=255)

                      tCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCtt                                                                                                                                 >  1:1302201/1‑150 (MQ=255)

                         ttttCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTa                                                                                                                              >  1:486435/1‑150 (MQ=255)

                         ttttCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTa                                                                                                                              >  1:399496/1‑150 (MQ=255)

                         ttttCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTa                                                                                                                              <  1:369499/150‑1 (MQ=255)

                            tCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGaa                                                                                                                           <  2:4504412/150‑1 (MQ=255)

                                cAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCg                                                                                                                       >  1:1194612/1‑150 (MQ=255)

                                      cgAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAAc                                                                                                                 <  2:645655/150‑1 (MQ=255)

                                       gAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAAcc                                                                                                                <  2:2527904/150‑1 (MQ=255)

                                        aGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCg                                                                                                               <  1:1386662/150‑1 (MQ=255)

                                         gCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCgg                                                                                                              <  2:4097396/150‑1 (MQ=255)

                                         gCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCgg                                                                                                              <  2:1787407/150‑1 (MQ=255)

                                             ggTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGcaca                                                                                                          >  1:4646293/1‑150 (MQ=255)

                                             ggTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGcaca                                                                                                          >  2:5118123/1‑150 (MQ=255)

                                                     tCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTg                                                                                                  >  2:4223035/1‑150 (MQ=255)

                                                                aGTGCCTTCAGGGGTGGCGCGAACCAGGTGCTACCGCGTCACCCCTTCATACCTGTCCAGCGTATCGCGTGCAAGCTCTCGCCCCCGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGAt                                                                                       <  2:3971499/150‑1 (MQ=255)

                                                                 gTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATc                                                                                      >  2:877816/1‑150 (MQ=255)

                                                                 gTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATc                                                                                      >  2:4685057/1‑150 (MQ=255)

                                                                  tGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCa                                                                                     <  1:4848745/150‑1 (MQ=255)

                                                                  tGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCa                                                                                     <  2:1836615/150‑1 (MQ=255)

                                                                   gCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAg                                                                                    >  1:4371317/1‑150 (MQ=255)

                                                                   gCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAg                                                                                    <  2:3787246/150‑1 (MQ=255)

                                                                      ttCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCGTGGGTACGTTGATCAGcac                                                                                 >  2:2668195/1‑150 (MQ=255)

                                                                            ggTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCgg                                                                           <  1:567242/150‑1 (MQ=255)

                                                                             gTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGt                                                                          <  2:2588081/150‑1 (MQ=255)

                                                                             gTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGt                                                                          >  2:3310026/1‑150 (MQ=255)

                                                                             gTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGt                                                                          <  2:3180179/150‑1 (MQ=255)

                                                                              tGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTg                                                                         <  1:2832068/150‑1 (MQ=255)

                                                                                  gcTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATa                                                                     >  2:1690403/1‑150 (MQ=255)

                                                                                  gcTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATa                                                                     >  2:2910648/1‑150 (MQ=255)

                                                                                  gcTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTGGGGTACGTTGATCAGCACATCCGGTGGATa                                                                     >  1:4828011/1‑150 (MQ=255)

                                                                                  gcTAACCAGGTGCTACCGCTTCACCCCTTCATAACCGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATa                                                                     >  2:810496/1‑150 (MQ=255)

                                                                                     aaCCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGcc                                                                  >  1:1540687/1‑150 (MQ=255)

                                                                                      aCCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCg                                                                 >  2:4319179/1‑150 (MQ=255)

                                                                                      aCCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCg                                                                 <  1:4413952/150‑1 (MQ=255)

                                                                                        cAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGc                                                               >  1:89672/1‑150 (MQ=255)

                                                                                        cAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGc                                                               >  2:2130891/1‑150 (MQ=255)

                                                                                              cTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCtt                                                         <  2:2888/150‑1 (MQ=255)

                                                                                              cTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCtt                                                         <  1:220318/150‑1 (MQ=255)

                                                                                               tACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTg                                                        >  2:669318/1‑150 (MQ=255)

                                                                                                 ccGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCTCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTa                                                      <  2:596776/150‑1 (MQ=255)

                                                                                                 ccGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTa                                                      <  1:2407815/150‑1 (MQ=255)

                                                                                                     ttCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGCCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACtgt                                                  <  2:4866353/150‑1 (MQ=255)

                                                                                                        aCCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGcc                                               >  2:1213532/1‑150 (MQ=255)

                                                                                                          cccTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCaa                                             <  1:4711795/150‑1 (MQ=255)

                                                                                                          cccTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCaa                                             >  1:4476998/1‑150 (MQ=255)

                                                                                                          cccTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCaa                                             >  2:1142960/1‑150 (MQ=255)

                                                                                                             ttCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGa                                          >  2:4048743/1‑150 (MQ=255)

                                                                                                              tCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGaa                                         <  2:2135711/150‑1 (MQ=255)

                                                                                                              tCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGaa                                         <  1:1547555/150‑1 (MQ=255)

                                                                                                                aTAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGt                                       <  2:2461953/150‑1 (MQ=255)

                                                                                                                  aaCTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCt                                     >  1:1727441/1‑150 (MQ=255)

                                                                                                                   aCTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTg                                    <  2:4646293/150‑1 (MQ=255)

                                                                                                                     tGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCa                                  <  2:3657029/150‑1 (MQ=255)

                                                                                                                     tGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCa                                  <  1:2713797/150‑1 (MQ=255)

                                                                                                                     tGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCa                                  >  1:997387/1‑150 (MQ=255)

                                                                                                                       tCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATg                                >  2:1628073/1‑150 (MQ=255)

                                                                                                                       tCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATg                                >  2:1628036/1‑150 (MQ=255)

                                                                                                                       tCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGAACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATg                                >  2:1112596/1‑150 (MQ=255)

                                                                                                                           gCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCt                            >  1:749934/1‑150 (MQ=255)

                                                                                                                             gTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCg                          >  2:2320482/1‑150 (MQ=255)

                                                                                                                               aTCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGaa                        <  2:3540016/150‑1 (MQ=255)

                                                                                                                               aTCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGaa                        <  2:1370844/150‑1 (MQ=255)

                                                                                                                                    gTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGctct                   <  1:1955274/150‑1 (MQ=255)

                                                                                                                                      gCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCTACGAAGTCTGCATGACCTCGAAGCTCTgg                 <  2:2406411/150‑1 (MQ=255)

                                                                                                                                       cAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGt                <  1:2573727/150‑1 (MQ=255)

                                                                                                                                       cAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGt                <  2:1996776/150‑1 (MQ=255)

                                                                                                                                        aaTCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTgg                 >  1:1485144/1‑148 (MQ=255)

                                                                                                                                        aaTCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTgg                 <  2:1485144/148‑1 (MQ=255)

                                                                                                                                        aaTCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGtt               >  1:3120711/1‑150 (MQ=255)

                                                                                                                                        aaTCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGtt               >  1:4341565/1‑150 (MQ=255)

                                                                                                                                        aaTCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGtt               >  2:3168389/1‑150 (MQ=255)

                                                                                                                                        aaTCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGtt               >  2:482132/1‑150 (MQ=255)

                                                                                                                                         aTCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTg              <  1:1251855/150‑1 (MQ=255)

                                                                                                                                          tctctcGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGa             <  2:1890956/150‑1 (MQ=255)

                                                                                                                                           tcatcGCCCTGGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGAt            >  2:3076457/4‑150 (MQ=255)

                                                                                                                                           tcatcGCCCTGGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGAt            >  2:3442233/4‑150 (MQ=255)

                                                                                                                                            catcGCCCTGGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGa             >  2:3827024/3‑148 (MQ=255)

                                                                                                                                            catcGCCCTGGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGa             <  1:3827024/146‑1 (MQ=255)

                                                                                                                                             ctcGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGATCa          >  2:3729057/1‑150 (MQ=255)

                                                                                                                                              tcGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGATCaa         >  1:202140/1‑150 (MQ=255)

                                                                                                                                               cGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGATCaaa        <  2:4995851/150‑1 (MQ=255)

                                                                                                                                               cGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGATCaaa        <  2:4371317/150‑1 (MQ=255)

                                                                                                                                               cGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGATCaaa        <  2:1302201/150‑1 (MQ=255)

                                                                                                                                                  cccAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGATCAAATcc     <  1:2979378/150‑1 (MQ=255)

                                                                                                                                                  cccAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGATCAAATcc     >  1:1043747/1‑150 (MQ=255)

                                                                                                                                                   ccAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTg                  <  1:1626283/136‑1 (MQ=255)

                                                                                                                                                     aGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGATCAAATCcagc  >  2:4574728/1‑150 (MQ=255)

AGGCCGCCTCGTGCGGCCTGTTTCGTTTTCGCCAGCCGCGAGCACGGTCATTGTCGAAGGTCACAGTGCCTTCAGGGGTGGCGCTAACCAGGTGCTACCGCTTCACCCCTTCATAACTGTCCAGCGTATCGCGTGCAATCTCTCGCCCCAGGGCGATGAGCTCCGGCGCCTTGTAGAACTCGAAAAACCGGCACACCCGCTTGGGTACGTTGATCAGCACATCCGGTGGATAGCCGGCAATCTTGTACTGTGCCAACGAAGTCTGCATGACCTCGAAGCTCTGGTTGATCAAATCCAGC  >  NC_002947/2637398‑2637696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`