BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,778,972	C→G	5.1%	W166C (TGG→TGC)	PP_2429 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,778,972	0	C	G	5.1%	465.4 / 11.0	138	W166C (TGG→TGC)	PP_2429	membrane protein
Reads supporting (aligned to +/- strand): ref base C (76/55); new base G (3/4); total (79/59)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 142 total aligned reads displayed.

TTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTT  >  NC_002947/2778822‑2779121

 ttCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCagcagc                                                                                                                                                        >  1:3223440/1‑150 (MQ=255)

 ttCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGc                                                                                                                                                        >  1:2820367/1‑150 (MQ=255)

    gcgAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCAc                                                                                                                                                     >  1:3633258/1‑150 (MQ=255)

    gcgAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCAc                                                                                                                                                     >  1:4626809/1‑150 (MQ=255)

        ggCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑ggg                                                                                                                                                <  2:1185875/150‑1 (MQ=255)

         gCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGGCTGCAGGCACC‑gggg                                                                                                                                               <  1:3767032/150‑2 (MQ=255)

         gCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCG‑CCCGGTGGCTGCAGGCACC‑ggggc                                                                                                                                              <  1:3887972/150‑3 (MQ=255)

          cGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCG‑CCCGGTGGCTGCAGGCACC‑ggggcg                                                                                                                                             <  2:2900339/150‑4 (MQ=255)

           gTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGgcgc                                                                                                                                             <  1:2365803/150‑1 (MQ=255)

           gTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGgcgc                                                                                                                                             >  1:2127712/1‑150 (MQ=255)

            ttGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCt                                                                                                                                            >  1:1838292/1‑150 (MQ=255)

             tGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTc                                                                                                                                           <  2:3621355/150‑1 (MQ=255)

              gACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCCGCCACC‑GGGCGCTcc                                                                                                                                          >  2:2332980/1‑150 (MQ=255)

              gACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTcc                                                                                                                                          >  2:2332787/1‑150 (MQ=255)

                   gTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCAcc                                                                                                                                     <  1:5081938/150‑1 (MQ=255)

                   gTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCAcc                                                                                                                                     <  1:5081925/150‑1 (MQ=255)

                   gTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCGCCCCAcc                                                                                                                                     >  1:3336716/1‑150 (MQ=255)

                    ttGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCAccc                                                                                                                                    >  2:4101758/1‑150 (MQ=255)

                         ccGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCACCCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCCac                                                                                                                               >  2:4434566/1‑150 (MQ=255)

                            aGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACAcc                                                                                                                            <  2:1620296/150‑1 (MQ=255)

                                       gCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAggcggc                                                                                                                 >  1:4435215/1‑150 (MQ=255)

                                       gCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAggcggc                                                                                                                 >  2:1944813/1‑150 (MQ=255)

                                       gCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAggcggc                                                                                                                 >  2:771282/1‑150 (MQ=255)

                                       gCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAggcggc                                                                                                                 <  1:3812784/150‑1 (MQ=255)

                                        ccccTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGcc                                                                                                                >  2:1374569/1‑150 (MQ=255)

                                          ccTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCAt                                                                                                              <  1:3969203/150‑1 (MQ=255)

                                          ccTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCAt                                                                                                              >  1:4895342/1‑150 (MQ=255)

                                            ttGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGc                                                                                                            <  2:3177397/150‑1 (MQ=255)

                                                 ggTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGc                                                                                                       <  2:2068689/150‑1 (MQ=255)

                                                 ggTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACA‑CACCCAGGCGGCCATGCTCGGCa                                                                                                      <  2:756242/150‑1 (MQ=255)

                                                   tCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCag                                                                                                     <  1:276452/150‑1 (MQ=255)

                                                   tCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCCCCCAGGCGGCCAAGCTCGGCag                                                                                                     >  1:879099/1‑150 (MQ=255)

                                                    cAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCaga                                                                                                    <  1:3818885/150‑1 (MQ=255)

                                                        tAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGa                                                                                                >  1:654608/1‑150 (MQ=255)

                                                        tAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGa                                                                                                <  2:2135510/150‑1 (MQ=255)

                                                         agagGCTCAGGCCAATACTGCAGCCCATGCCGAGATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCTGCCGAGcatg                                                                                               <  2:2576244/150‑4 (MQ=255)

                                                           agGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCa                                                                                             <  2:2127712/150‑1 (MQ=255)

                                                             gCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCCCCCCCAACACCGCCCAGGCGGCCATGCTCGGCCGAGCGATCAGc                                                                                           >  1:4529734/1‑150 (MQ=255)

                                                               tCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCaa                                                                                         >  1:735438/1‑150 (MQ=255)

                                                                 aGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCCGGCGGCCCTGCTCGGCAGAGCGATCAGCAAGa                                                                                       >  1:177047/1‑150 (MQ=255)

                                                                      aaTACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACAcc                                                                                  >  1:105352/1‑150 (MQ=255)

                                                                       aTACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCa                                                                                 <  1:4033433/150‑1 (MQ=255)

                                                                        tACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAg                                                                                <  1:3522554/150‑1 (MQ=255)

                                                                           tGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCCCC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAgcgc                                                                             >  1:2401265/1‑150 (MQ=255)

                                                                            gCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGcc                                                                            >  1:3797254/1‑150 (MQ=255)

                                                                               gCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCCACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGc                                                                         >  1:1007406/1‑150 (MQ=255)

                                                                                cccATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCt                                                                        <  1:3627762/150‑1 (MQ=255)

                                                                                  cATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGc                                                                      <  2:3782099/150‑1 (MQ=255)

                                                                                     gCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCcac                                                                   >  2:82101/1‑150 (MQ=255)

                                                                                     gCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCcac                                                                   >  1:2967127/1‑150 (MQ=255)

                                                                                      ccGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCGCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCcaca                                                                  >  1:3104402/1‑150 (MQ=255)

                                                                                          tatCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCCGCTGCCACACTgg                                                              >  1:1675034/1‑150 (MQ=255)

                                                                                            tCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTTCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCa                                                            <  2:4507843/150‑1 (MQ=255)

                                                                                              aacaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAac                                                          >  1:918184/1‑150 (MQ=255)

                                                                                              aacaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAac                                                          >  2:3629504/1‑150 (MQ=255)

                                                                                               acaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCCAGAACACCAGCGCCAGCTGCCACACTGGCAacg                                                         >  2:797165/1‑150 (MQ=255)

                                                                                                caaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCTCTCCCCACCCCCCACACCGCCCAGGCGGCCAAGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACAGGCAacga                                                        >  1:904206/1‑150 (MQ=255)

                                                                                                caaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAacga                                                        >  2:1704725/1‑150 (MQ=255)

                                                                                                caaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACAGGCAacga                                                        >  1:933089/1‑150 (MQ=255)

                                                                                                  aaaCCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCCCC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAacgacg                                                      >  2:53800/1‑150 (MQ=255)

                                                                                                  aaaCCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAacgacg                                                      <  1:1081266/150‑1 (MQ=255)

                                                                                                         cGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGc                                               <  2:654608/150‑1 (MQ=255)

                                                                                                         cGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCGCGGCAGAGCGATCAGCACGAACACCAGCGCCCGCTGCCACACTGGCACCGACGGCGCGGc                                               >  1:470450/1‑150 (MQ=255)

                                                                                                          gCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCa                                              >  1:1460775/1‑150 (MQ=255)

                                                                                                            cAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCaaa                                            >  2:381342/1‑150 (MQ=255)

                                                                                                                acTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATAcc                                        >  1:1986203/1‑150 (MQ=255)

                                                                                                                 cTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCCGCTGCCACACTGGCAACGACGGCGCGGCAAATAccc                                       >  1:1694139/1‑150 (MQ=255)

                                                                                                                  ttGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCATCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATAcccc                                      >  2:1573420/1‑150 (MQ=255)

                                                                                                                    ggTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCCAATACCCCGa                                    >  2:3780553/1‑150 (MQ=255)

                                                                                                                    ggTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGa                                    >  2:1271137/1‑150 (MQ=255)

                                                                                                                     gTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGac                                   >  2:633491/1‑150 (MQ=255)

                                                                                                                        gAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCCGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCACATACCCCGaccac                                >  2:168029/1‑150 (MQ=255)

                                                                                                                        gAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGaccac                                >  2:4556364/1‑150 (MQ=255)

                                                                                                                          acacCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCACCGACGGCGCGGCAAATACCCCGACCACCg                              >  2:167280/1‑150 (MQ=255)

                                                                                                                           cacCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGc                             <  2:3820174/150‑1 (MQ=255)

                                                                                                                              ccGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCAc                          >  1:2690497/1‑150 (MQ=255)

                                                                                                                               cGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACg                         >  2:3233589/1‑150 (MQ=255)

                                                                                                                                  aaCCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGACAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGcc                      >  1:2007315/1‑150 (MQ=255)

                                                                                                                                   gagcgcccGGTGGCTGCAGGCACCGGGGCGGTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCCCACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGcc                      >  2:2910335/6‑150 (MQ=255)

                                                                                                                                   aCCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCa                     <  1:2752114/150‑1 (MQ=255)

                                                                                                                                     cGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATc                   <  1:279415/150‑1 (MQ=255)

                                                                                                                                      gCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCa                  >  1:2934490/1‑150 (MQ=255)

                                                                                                                                       ccccGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCaa                 <  1:1374569/150‑1 (MQ=255)

                                                                                                                                        cccGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAAc                <  1:781348/150‑1 (MQ=255)

                                                                                                                                        cccGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAAc                <  2:3223440/150‑1 (MQ=255)

                                                                                                                                        cccGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAAc                <  2:2820367/150‑1 (MQ=255)

                                                                                                                                         ccGGTGGCTGCAGGCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCCGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCCCACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAAcc               >  2:4727420/1‑150 (MQ=255)

                                                                                                                                         ccGGTGGCTGCAGGCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAAcc               >  1:4075658/1‑150 (MQ=255)

                                                                                                                                            gTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGa            >  1:2590191/1‑150 (MQ=255)

                                                                                                                                                cTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCtt        <  2:183670/150‑1 (MQ=255)

                                                                                                                                                 tGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTg       <  2:1397267/150‑1 (MQ=255)

                                                                                                                                                  gCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTgg      <  2:3797251/150‑1 (MQ=255)

                                                                                                                                                   cAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTggg     >  2:1186383/1‑150 (MQ=255)

                                                                                                                                                   cAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTggg     <  1:262358/150‑1 (MQ=255)

                                                                                                                                                   cAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATAACCCGACCACCCCCACGGCCAACAACCAGACCATggg     >  1:3565822/1‑150 (MQ=255)

                                                                                                                                                    aGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTgggg    <  1:1775378/150‑1 (MQ=255)

                                                                                                                                                    aGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTgggg    >  2:4439503/1‑150 (MQ=255)

                                                                                                                                                    aGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTgggg    <  1:1944813/150‑1 (MQ=255)

                                                                                                                                                    aGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTgggg    <  2:4895342/150‑1 (MQ=255)

                                                                                                                                                      ccACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGtt  <  1:1271137/150‑1 (MQ=255)

TTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTT  >  NC_002947/2778822‑2779121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`