Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,960,764 G→C 16.7% intergenic (+65/+77) PP_2589 → / ← PP_2590 aldehyde dehydrogenase family protein/ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,960,7640GC16.7% 325.1 / 58.4 132intergenic (+65/+77)PP_2589/PP_2590aldehyde dehydrogenase family protein/ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base G (70/39);  new base C (8/14);  total (78/54)
Fisher's exact test for biased strand distribution p-value = 1.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-02

Only 100 of 132 total aligned reads displayed.

TCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGG  >  NC_002947/2960616‑2960909
                                                                                                                                                    |                                                                                                                                                 
tCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGt                                                                                                                                                  <  1:3601811/150‑1 (MQ=255)
tCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGt                                                                                                                                                  >  1:1909988/1‑150 (MQ=255)
   gggTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATAATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTg                                                                                                                                               >  1:4511396/1‑150 (MQ=255)
    ggTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGa                                                                                                                                              <  1:3408100/150‑1 (MQ=255)
     gTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAg                                                                                                                                             <  1:91151/150‑1 (MQ=255)
     gTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAg                                                                                                                                             <  1:2644897/150‑1 (MQ=255)
      tttGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAgg                                                                                                                                            >  1:1106484/1‑150 (MQ=255)
      tttGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAgg                                                                                                                                            >  2:1100019/1‑150 (MQ=255)
         ggCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGtt                                                                                                                                         <  2:3546207/150‑1 (MQ=255)
            cgcgATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTtgtg                                                                                                                                      >  1:3174305/1‑150 (MQ=255)
               gATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGAGGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACACGTCTGAGGGTTGTGCTg                                                                                                                                   <  2:4007875/150‑1 (MQ=255)
                aTCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTga                                                                                                                                  <  2:1301662/150‑2 (MQ=38)
                aTCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTga                                                                                                                                  <  2:1302040/150‑2 (MQ=38)
                aTCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTga                                                                                                                                  <  1:4192570/150‑2 (MQ=38)
                 tCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGa                                                                                                                                 <  2:1349481/150‑1 (MQ=255)
                 tCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGa                                                                                                                                 <  1:4039656/150‑1 (MQ=255)
                 tCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTgaa                                                                                                                                 <  1:2043928/150‑3 (MQ=38)
                  cTGTCACTGCATTCGTTCGACAAGTATTCGCTACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAAc                                                                                                                                <  2:900937/150‑1 (MQ=37)
                  cTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTTTTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAAc                                                                                                                                <  2:901187/150‑1 (MQ=37)
                  cTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGAc                                                                                                                                <  2:1573844/150‑1 (MQ=255)
                    gTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTTGCGGGCGCGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCt                                                                                                                              <  1:2056586/150‑1 (MQ=255)
                    gTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCt                                                                                                                              <  2:3823018/150‑1 (MQ=255)
                    gTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCt                                                                                                                              >  2:2942741/1‑150 (MQ=255)
                    gTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAACCt                                                                                                                              <  2:4686678/150‑1 (MQ=37)
                      cACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCtgt                                                                                                                            <  1:1792848/150‑1 (MQ=255)
                      cACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCtgt                                                                                                                            <  1:4068400/150‑1 (MQ=255)
                      cACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAACCtgt                                                                                                                            <  1:2591202/150‑1 (MQ=37)
                      cACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAACCtgt                                                                                                                            <  1:2591277/150‑1 (MQ=37)
                       aCTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCtgtg                                                                                                                           >  2:4795552/1‑150 (MQ=255)
                        cTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTgg                                                                                                                          <  2:508164/150‑1 (MQ=255)
                        cTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTgg                                                                                                                          >  2:3926096/1‑150 (MQ=255)
                        cTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTgg                                                                                                                          >  2:3925974/1‑150 (MQ=255)
                        cTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGGTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAACCTGTgg                                                                                                                          <  2:243321/150‑1 (MQ=37)
                              tcgttcgACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGGTCAGCACAAGTCTGAGGGTTGTGCTGGACCGGTGGGAGCgg                                                                                                                    >  1:2371872/1‑150 (MQ=255)
                                    gACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAggg                                                                                                                                           <  1:1414155/121‑1 (MQ=255)
                                    gACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGgcgcgc                                                                                                              >  1:1359039/1‑150 (MQ=255)
                                    gACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGAGGGAGCGGgcgcgc                                                                                                              >  1:4025785/1‑150 (MQ=255)
                                     aCAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGa                                                                                                                                              <  2:3523598/117‑1 (MQ=255)
                                     aCAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGcc                                                                                                             >  1:4522118/1‑150 (MQ=255)
                                     aCAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGcc                                                                                                             >  1:4522143/1‑150 (MQ=255)
                                     aCAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGcc                                                                                                             >  1:4522198/1‑150 (MQ=255)
                                      cAAGTATTCGCAACTCAAGCCCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGccc                                                                                                            <  2:4096843/150‑1 (MQ=255)
                                       aaGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCcg                                                                                                           >  1:607643/1‑150 (MQ=255)
                                           aTTCCCAACCCACGACCACCTGGTACCATGGGCGCGGCTGATTTATTGATCATTGCCCGCGTCGTCTGCTTCGCGGGCAGGCCCGCTCCCCCTGGTTCCGCACAAGACTGAGGGTTGTGCTGCACCTGTGGGAGCGGGCGCGCCCGCGaa                                                                                                       <  1:2465139/150‑1 (MQ=37)
                                            ttCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAag                                                                                                      >  1:1658512/1‑150 (MQ=255)
                                            ttCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAag                                                                                                      >  2:2602825/1‑150 (MQ=255)
                                               gCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAgg                                                                                                   >  2:791999/1‑150 (MQ=255)
                                               gCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCACGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACGTGTGGGAGCGGGCGCGCCCGCGAAGTgg                                                                                                   >  2:1947244/1‑150 (MQ=255)
                                                cAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGc                                                                                                  <  2:1106484/150‑1 (MQ=255)
                                                 aaCTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGcc                                                                                                 >  2:1297895/1‑150 (MQ=255)
                                                    tCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCGGAGGGGTGTGCTGGCCCAGTGGGAGCGGGCGCGCCCGCGACGAGGCCGTa                                                                                              >  1:4733555/1‑150 (MQ=39)
                                                           caccTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGCGGCCGTATCTGgcg                                                                                       >  1:2227140/1‑150 (MQ=255)
                                                               tGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCGGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGcgccgc                                                                                   >  1:2286045/1‑150 (MQ=255)
                                                                   aCCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGCAGAGGCCGTATCTGGCGCCgcggcg                                                                               >  1:3210829/1‑150 (MQ=255)
                                                                     cAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCGGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCggg                                                                             >  2:4030055/1‑150 (MQ=255)
                                                                       gTTGCGCGGCTTATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCCGCACAAGTCGGAGGGTTGTGCTGGACCGGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCgcggcgccag                                                                           <  2:1498884/150‑5 (MQ=39)
                                                                       gTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGAc                                                                           <  2:3378205/150‑1 (MQ=255)
                                                                              gggtgttgttttgtttATTGACCGCGTCTTCTGCTTGGGGGGCTTGCCCGCTCCCACTGGTTCTGCACTTGTCTGTGGGTTGTGCTGGTCGTGTGGGTGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGTCCCGGTTa                                                                    <  2:2979968/144‑1 (MQ=255)
                                                                              ggCTTATGTTTTGATCTTTGCCGGCGTCGTCTGCTTCGCGGGCAGGCCCGCTCCCCCCGGTTCAGCACACGTCTGTGGGTTGTGGTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTa                                                                    <  2:1909988/150‑1 (MQ=38)
                                                                              ggCTGATTTTTTTATCATTGACCGGGTCGTCTGCTTGGCGGGCATGCCCGCCCCCACCGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTa                                                                    <  2:1910022/150‑1 (MQ=39)
                                                                              ggCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGGATCTGGCGCCGCGGCGGGACCCGGTTa                                                                    >  2:3198598/1‑150 (MQ=255)
                                                                              ggCTGATGATTTGATCATTGACCGCGTCGTCTGCTTCGGGGGCATGCCCGCTCCCACAGGTTCAGCAAATGTGTTAGGTTTGTGCTGGACCTTTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTa                                                                    <  2:4511396/150‑1 (MQ=38)
                                                                                   aTGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGGATCTGGCGCCGCGGCGGGGCCCCGGTAGAAga                                                               >  1:3919143/1‑149 (MQ=255)
                                                                                      tATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCa                                                            >  1:2595955/1‑150 (MQ=255)
                                                                                        ttGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGGCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGGTGGACCTGTGGGAGCGGGCGCGGCCGCGTAGTGGCCGTATCTGGGGCCGGGGCGGGACCCGGTTAGAAGTACACg                                                          >  1:1899359/1‑150 (MQ=255)
                                                                                         tGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCCCTGGTTCCGCACAAGTCTGAGGGTTGGGCTGGACCTGTGGGCGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGc                                                         <  2:2597127/150‑1 (MQ=255)
                                                                                                   ccGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACCGGTTCAGCACACGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAg                                               <  2:2120342/150‑1 (MQ=255)
                                                                                                    cgcgTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCCGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATATGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGc                                              >  1:1111512/1‑150 (MQ=255)
                                                                                                    cgcgTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCGGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCCGTTAGCAGTCCACGCTAGCCGCAAGc                                              >  1:2256481/1‑150 (MQ=255)
                                                                                                       gtcgtcTGCTTCGCGGGCTTGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGa                                           <  2:246820/150‑1 (MQ=255)
                                                                                                       gtcgtcTGCTTCGCGGGCATGCCCGCGCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCGGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGa                                           <  2:4456679/150‑1 (MQ=255)
                                                                                                       ggcgtcGGCTTCGCGGGCTGGCCCGCGCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGa                                           <  1:2942741/148‑1 (MQ=255)
                                                                                                           tcTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAgg                                       >  2:1537814/1‑150 (MQ=255)
                                                                                                            cTGCTTCGCGGGCATGCCCGCTGCCACAGGTTCAGCACAAGTCTGAGGGTGGGGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGt                                      >  2:23896/1‑150 (MQ=255)
                                                                                                            cTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGt                                      >  2:2577954/1‑150 (MQ=255)
                                                                                                                         aTGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGccccc                         >  1:3689988/1‑150 (MQ=255)
                                                                                                                             ccGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAAcc                     >  2:4733238/1‑150 (MQ=255)
                                                                                                                              cGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAAccc                    >  2:1712804/1‑150 (MQ=255)
                                                                                                                              cGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAAccc                    >  2:1712706/1‑150 (MQ=255)
                                                                                                                                 tCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAg                 >  1:5042290/1‑150 (MQ=37)
                                                                                                                                  cccACAGGGTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAgg                <  2:2072450/150‑1 (MQ=255)
                                                                                                                                   ccACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCGGATAGGTCCGCGGCGCCCCCAACCCCAggg               >  1:2315611/1‑150 (MQ=255)
                                                                                                                                   ccACAGGTCCAGCACAACCCTCTGACGTGTGCTGAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAggg               >  2:4286071/1‑150 (MQ=37)
                                                                                                                                    cacaGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGt              >  1:2710287/1‑150 (MQ=255)
                                                                                                                                    cacaGGTCCAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGAGAGGTCCGCGGCGCCCCCAACCCCAGGGt              >  2:2919204/1‑150 (MQ=25)
                                                                                                                                     acaGGTCCAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCGCGCCCCCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTACGCGGCGCCCCCAACCCCAGGGTc             >  1:1257107/1‑150 (MQ=37)
                                                                                                                                       aGGTCCAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCATGCCCGCGAAGCGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATATGTCCGCGGCGCCCCCAACCCCAGGGTCTg           >  2:1789322/1‑150 (MQ=25)
                                                                                                                                         gtccAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTggg         >  2:4200592/4‑150 (MQ=37)
                                                                                                                                         gTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTggg         >  2:4048411/1‑150 (MQ=255)
                                                                                                                                         gTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGGTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTggg         >  1:59568/1‑150 (MQ=255)
                                                                                                                                          tccAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGTCGCCCCCAACCCCAGGGTCTGGGt        >  1:4182234/3‑150 (MQ=37)
                                                                                                                                          tccAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGt        >  1:3457255/3‑150 (MQ=25)
                                                                                                                                            cAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCa      >  1:240160/1‑150 (MQ=255)
                                                                                                                                             aGCACACGTCGGAGGGTTGTGCTGGACCTGTGGGAGGGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAg     <  2:1111512/150‑1 (MQ=255)
                                                                                                                                             aGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAg     >  2:1454427/1‑150 (MQ=255)
                                                                                                                                             aGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAg     >  1:4704305/1‑150 (MQ=255)
                                                                                                                                               cacaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTg   >  2:3686274/1‑150 (MQ=255)
                                                                                                                                               cacaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTg   >  2:3686446/1‑150 (MQ=255)
                                                                                                                                                acaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGtggccaa  >  2:4888030/1‑144 (MQ=255)
                                                                                                                                                acaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTgg  >  1:1384546/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGG  >  NC_002947/2960616‑2960909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑