Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,960,768 G→C 17.1% intergenic (+69/+73) PP_2589 → / ← PP_2590 aldehyde dehydrogenase family protein/ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,960,7680GC17.1% 323.4 / 58.7 129intergenic (+69/+73)PP_2589/PP_2590aldehyde dehydrogenase family protein/ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base G (69/37);  new base C (8/14);  total (77/52)
Fisher's exact test for biased strand distribution p-value = 1.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.36e-01

Only 100 of 130 total aligned reads displayed.

GGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGCACACCC  >  NC_002947/2960619‑2960916
                                                                                                                                                     |                                                                                                                                                    
gggTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATAATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTg                                                                                                                                                      >  1:4511396/1‑150 (MQ=255)
 ggTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGa                                                                                                                                                     <  1:3408100/150‑1 (MQ=255)
  gTTTGGCCGCGATCTGTCTCTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAg                                                                                                                                                    <  1:2243896/150‑1 (MQ=255)
  gTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAg                                                                                                                                                    <  1:91151/150‑1 (MQ=255)
  gTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAg                                                                                                                                                    <  1:2644897/150‑1 (MQ=255)
   tttGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAgg                                                                                                                                                   >  1:1106484/1‑150 (MQ=255)
      ggCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGtt                                                                                                                                                <  2:3546207/150‑1 (MQ=255)
         cgcgATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTtgtg                                                                                                                                             >  1:3174305/1‑150 (MQ=255)
             aTCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTga                                                                                                                                         <  2:1302040/150‑2 (MQ=38)
             aTCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTga                                                                                                                                         <  2:1301662/150‑2 (MQ=38)
             aTCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTga                                                                                                                                         <  1:4192570/150‑2 (MQ=38)
              tCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGa                                                                                                                                        <  2:1349481/150‑1 (MQ=255)
              tCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGa                                                                                                                                        <  1:4039656/150‑1 (MQ=255)
               cTGTCACTGCATTCGTTCGACAAGTATTCGCTACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAAc                                                                                                                                       <  2:900937/150‑1 (MQ=37)
               cTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTTTTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAAc                                                                                                                                       <  2:901187/150‑1 (MQ=37)
               cTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAAc                                                                                                                                       <  2:175092/150‑1 (MQ=37)
                 gTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTTGCGGGCGCGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCt                                                                                                                                     <  1:2056586/150‑1 (MQ=255)
                 gTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCt                                                                                                                                     >  2:2942741/1‑150 (MQ=255)
                 gTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCt                                                                                                                                     <  2:3823018/150‑1 (MQ=255)
                 gTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAACCt                                                                                                                                     <  2:4686678/150‑1 (MQ=37)
                   cACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCtgt                                                                                                                                   <  1:1792848/150‑1 (MQ=255)
                   cACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCtgt                                                                                                                                   <  1:4068400/150‑1 (MQ=255)
                   cACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAACCtgt                                                                                                                                   <  1:2591202/150‑1 (MQ=37)
                   cACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAACCtgt                                                                                                                                   <  1:4122617/150‑1 (MQ=37)
                    aCTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCtgtg                                                                                                                                  >  2:4795552/1‑150 (MQ=255)
                    aCTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGGTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAACCtgtg                                                                                                                                  <  2:2774584/150‑1 (MQ=37)
                     cTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTgg                                                                                                                                 <  2:508164/150‑1 (MQ=255)
                     cTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTgg                                                                                                                                 >  2:3925974/1‑150 (MQ=255)
                     cTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTgg                                                                                                                                 >  2:3926096/1‑150 (MQ=255)
                     cTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGGTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAACCTGTgg                                                                                                                                 <  2:243321/150‑1 (MQ=37)
                           tcgttcgACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGGTCAGCACAAGTCTGAGGGTTGTGCTGGACCGGTGGGAGCgg                                                                                                                           >  1:2371872/1‑150 (MQ=255)
                                 gACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAggg                                                                                                                                                  >  2:1414155/1‑121 (MQ=255)
                                 gACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAggg                                                                                                                                                  <  1:1414155/121‑1 (MQ=255)
                                 gACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGgcgcgc                                                                                                                     >  1:1359039/1‑150 (MQ=255)
                                 gACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGAGGGAGCGGgcgcgc                                                                                                                     >  1:4025785/1‑150 (MQ=255)
                                  aCAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGcc                                                                                                                    >  1:4522088/1‑150 (MQ=255)
                                  aCAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGcc                                                                                                                    >  1:4522143/1‑150 (MQ=255)
                                  aCAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGcc                                                                                                                    >  1:4522198/1‑150 (MQ=255)
                                  aCAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGcc                                                                                                                    >  2:4502236/1‑150 (MQ=255)
                                  aCAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACACGTTCAGCACAAGTCTGa                                                                                                                                                     >  1:3523598/1‑117 (MQ=255)
                                    aaGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCcg                                                                                                                  >  1:607643/1‑150 (MQ=255)
                                        tttCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTTATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGTTTCAGCACAAGTCTGTGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGaa                                                                                                              <  1:5122271/149‑1 (MQ=255)
                                        aTTCCCAACCCACGACCACCTGGTACCATGGGCGCGGCTGATTTATTGATCATTGCCCGCGTCGTCTGCTTCGCGGGCAGGCCCGCTCCCCCTGGTTCCGCACAAGACTGAGGGTTGTGCTGCACCTGTGGGAGCGGGCGCGCCCGCGaa                                                                                                              <  1:2465139/150‑1 (MQ=37)
                                            gCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAgg                                                                                                          >  2:791999/1‑150 (MQ=255)
                                            gCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCACGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACGTGTGGGAGCGGGCGCGCCCGCGAAGTgg                                                                                                          >  2:1947244/1‑150 (MQ=255)
                                             cAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGc                                                                                                         <  2:1106484/150‑1 (MQ=255)
                                                 tCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCGGAGGGGTGTGCTGGCCCAGTGGGAGCGGGCGCGCCCGCGACGAGGCCGTa                                                                                                     >  1:4733555/1‑150 (MQ=39)
                                                        caccTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGCGGCCGTATCTGgcg                                                                                              >  1:2227140/1‑150 (MQ=255)
                                                                aCCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGCAGAGGCCGTATCTGGCGCCgcggcg                                                                                      >  1:3210829/1‑150 (MQ=255)
                                                                 ccAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGCAGAGGCCGTATCTGGCGCCCcggcgg                                                                                     >  1:2621092/1‑150 (MQ=255)
                                                                  cAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCGGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCggg                                                                                    >  2:4030055/1‑150 (MQ=255)
                                                                    gTTGCGCGGCTTATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCCGCACAAGTCGGAGGGTTGTGCTGGACCGGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCgcggcgccag                                                                                  <  2:1498884/150‑5 (MQ=39)
                                                                    gTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGAc                                                                                  <  2:3378205/150‑1 (MQ=255)
                                                                           gggtgttgttttgtttATTGACCGCGTCTTCTGCTTGGGGGGCTTGCCCGCTCCCACTGGTTCTGCACTTGTCTGTGGGTTGTGCTGGTCGTGTGGGTGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGTCCCGGTTa                                                                           <  2:2979968/144‑1 (MQ=255)
                                                                           ggCTTATGTTTTGATCTTTGCCGGCGTCGTCTGCTTCGCGGGCAGGCCCGCTCCCCCCGGTTCAGCACACGTCTGTGGGTTGTGGTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTa                                                                           <  2:1909988/150‑1 (MQ=38)
                                                                           ggCTGATTTTTTTATCATTGACCGGGTCGTCTGCTTGGCGGGCATGCCCGCCCCCACCGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTa                                                                           <  2:1910022/150‑1 (MQ=39)
                                                                           ggCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGGATCTGGCGCCGCGGCGGGACCCGGTTa                                                                           >  2:3198598/1‑150 (MQ=255)
                                                                           ggCTGATGATTTGATCATTGACCGCGTCGTCTGCTTCGGGGGCATGCCCGCTCCCACAGGTTCAGCAAATGTGTTAGGTTTGTGCTGGACCTTTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTa                                                                           <  2:4511396/150‑1 (MQ=38)
                                                                            tcttttttATTTATCTTTGACCGGGTCGTCTGCTTCGCGGGCTTGCCCGCTCCCACTGTTTCAGCACAAGTCTGTGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAg                                                                          <  2:3919143/143‑1 (MQ=37)
                                                                                aTGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGGATCTGGCGCCGCGGCGGGGCCCCGGTAGAAga                                                                      >  1:3919143/1‑149 (MQ=255)
                                                                                   tATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCa                                                                   >  1:2595955/1‑150 (MQ=255)
                                                                                     ttGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGGCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGGTGGACCTGTGGGAGCGGGCGCGGCCGCGTAGTGGCCGTATCTGGGGCCGGGGCGGGACCCGGTTAGAAGTACACg                                                                 >  1:1899359/1‑150 (MQ=255)
                                                                                      tGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCCCTGGTTCCGCACAAGTCTGAGGGTTGGGCTGGACCTGTGGGCGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGc                                                                <  2:2597127/150‑1 (MQ=255)
                                                                                         tCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAg                                                             >  2:2477294/1‑150 (MQ=255)
                                                                                                 cgcgTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCCGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATATGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGc                                                     >  1:1111512/1‑150 (MQ=255)
                                                                                                 cgcgTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCGGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCCGTTAGCAGTCCACGCTAGCCGCAAGc                                                     >  1:2256481/1‑150 (MQ=255)
                                                                                                        tcTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAgg                                              >  2:1537814/1‑150 (MQ=255)
                                                                                                         cTGCTTCGCGGGCATGCCCGCTGCCACAGGTTCAGCACAAGTCTGAGGGTGGGGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGt                                             >  2:23896/1‑150 (MQ=255)
                                                                                                         cTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGt                                             >  2:2577954/1‑150 (MQ=255)
                                                                                                                      aTGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGccccc                                >  1:2538927/1‑150 (MQ=255)
                                                                                                                      aTGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGccccc                                >  1:3689988/1‑150 (MQ=255)
                                                                                                                          ccGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAAcc                            >  2:4733238/1‑150 (MQ=255)
                                                                                                                           cGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAAccc                           >  2:1712804/1‑150 (MQ=255)
                                                                                                                           cGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAAccc                           >  2:1712706/1‑150 (MQ=255)
                                                                                                                              tCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAg                        >  1:5042290/1‑150 (MQ=37)
                                                                                                                               cccACAGGGTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAgg                       <  2:2072450/150‑1 (MQ=255)
                                                                                                                                ccACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAggg                      >  1:2315693/1‑150 (MQ=255)
                                                                                                                                ccACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAggg                      >  2:4195280/1‑150 (MQ=255)
                                                                                                                                ccACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAggg                      >  2:1050637/1‑150 (MQ=255)
                                                                                                                                ccACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCGGATAGGTCCGCGGCGCCCCCAACCCCAggg                      >  1:2315611/1‑150 (MQ=255)
                                                                                                                                ccACAGGTCCAGCACAACCCTCTGACGTGTGCTGAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAggg                      >  2:4286071/1‑150 (MQ=37)
                                                                                                                                 cacaGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGt                     >  1:2710287/1‑150 (MQ=255)
                                                                                                                                 cacaGGTCCAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGAGAGGTCCGCGGCGCCCCCAACCCCAGGGt                     >  2:2919204/1‑150 (MQ=25)
                                                                                                                                  acaGGTCCAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCGCGCCCCCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTACGCGGCGCCCCCAACCCCAGGGTc                    >  1:1257107/1‑150 (MQ=37)
                                                                                                                                      gtccAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTggg                >  2:4200592/4‑150 (MQ=37)
                                                                                                                                      gTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTggg                >  2:4048411/1‑150 (MQ=255)
                                                                                                                                       ttCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAATTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGt               >  1:4670002/1‑150 (MQ=255)
                                                                                                                                        tCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTc              >  1:1755478/1‑150 (MQ=255)
                                                                                                                                          aGCACACGTCGGAGGGTTGTGCTGGACCTGTGGGAGGGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAg            <  2:1111512/150‑1 (MQ=255)
                                                                                                                                           gCACAAGTCTGAGGGTGGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGt           <  2:4024535/150‑1 (MQ=255)
                                                                                                                                            cacaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTg          >  2:3686274/1‑150 (MQ=255)
                                                                                                                                            cacaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTg          >  2:3686232/1‑150 (MQ=255)
                                                                                                                                            cacaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTg          >  2:1121917/1‑150 (MQ=255)
                                                                                                                                             acaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGtggccaa         >  2:4888030/1‑144 (MQ=255)
                                                                                                                                             acaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTgg         >  1:1384546/1‑150 (MQ=255)
                                                                                                                                             acaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTgg         >  2:1542248/1‑150 (MQ=255)
                                                                                                                                             acaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTgg         >  2:2145992/1‑150 (MQ=255)
                                                                                                                                                  tCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGCacac    >  2:3234140/1‑150 (MQ=255)
                                                                                                                                                    tGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGCCCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCACGGTCTGGGTCAGTGGCACAccc  >  1:4709173/1‑150 (MQ=255)
                                                                                                                                                    tGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGCACAccc  >  1:1428047/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGCACACCC  >  NC_002947/2960619‑2960916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑