BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,008,439	A→C	7.0%	K1019Q (AAG→CAG)	PP_2627 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,008,439	0	A	C	7.0%	575.4 / 22.3	175	K1019Q (AAG→CAG)	PP_2627	membrane protein
Reads supporting (aligned to +/- strand): ref base A (86/75); new base C (8/5); total (95/80)
Fisher's exact test for biased strand distribution p-value = 7.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

ACGTGTACAGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAGCCC  >  NC_002947/3008290‑3008588

 cGTGTACAGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGaa                                                                                                                                                      <  1:2730132/150‑1 (MQ=255)

 cGTGTACAGATATTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTACTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGaa                                                                                                                                                      <  2:3841705/150‑1 (MQ=255)

     tACAGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGAc                                                                                                                                                  >  1:927808/1‑150 (MQ=255)

     tACAGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGAc                                                                                                                                                  >  1:2998757/1‑150 (MQ=255)

     tACAGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGAc                                                                                                                                                  <  2:4220926/150‑1 (MQ=255)

     tACAGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGAc                                                                                                                                                  >  2:2054635/1‑150 (MQ=255)

       cAGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACaa                                                                                                                                                >  2:1049752/1‑150 (MQ=255)

       cAGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACaa                                                                                                                                                <  2:2998557/150‑1 (MQ=255)

        aGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAAc                                                                                                                                               <  2:4062819/150‑1 (MQ=255)

        aGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAAc                                                                                                                                               >  1:4452584/1‑150 (MQ=255)

           tttttCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTGTCCTTCAGGAAAACCtt                                                                                                                                            <  1:4814167/150‑1 (MQ=255)

             tttCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGa                                                                                                                                          <  2:2866196/150‑1 (MQ=255)

             tttCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGa                                                                                                                                          <  1:696719/150‑1 (MQ=255)

             tttCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGa                                                                                                                                          <  1:4441150/150‑1 (MQ=255)

             tttCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTGTCCTTCAGGAAAACCTTGa                                                                                                                                          <  1:377991/150‑1 (MQ=255)

              ttCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAg                                                                                                                                         <  1:1352991/150‑1 (MQ=255)

              ttCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAg                                                                                                                                         <  1:391636/150‑1 (MQ=255)

               tCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAgg                                                                                                                                        >  1:2455957/1‑150 (MQ=255)

                 cAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGcc                                                                                                                                      >  1:1080362/1‑150 (MQ=255)

                     aaCGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGc                                                                                                                                  >  2:3246814/1‑150 (MQ=255)

                       cGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAg                                                                                                                                <  1:3350918/150‑1 (MQ=255)

                        gCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGt                                                                                                                               >  1:4353689/1‑150 (MQ=255)

                        gCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGt                                                                                                                               >  2:2601544/1‑150 (MQ=255)

                        gCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGt                                                                                                                               >  1:1587912/1‑150 (MQ=255)

                          cTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTcc                                                                                                                             >  1:551037/1‑150 (MQ=255)

                            ggCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGc                                                                                                                           >  2:85888/1‑150 (MQ=255)

                               aGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCa                                                                                                                        <  2:4741345/150‑1 (MQ=255)

                                ggCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAg                                                                                                                       >  1:2501210/1‑150 (MQ=255)

                                ggCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAg                                                                                                                       >  1:5070870/1‑150 (MQ=255)

                                ggCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAg                                                                                                                       <  2:4617275/150‑1 (MQ=255)

                                 gCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGc                                                                                                                      >  1:2092795/1‑150 (MQ=255)

                                          ccTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAAt                                                                                                             >  2:1162717/1‑150 (MQ=255)

                                           cTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATc                                                                                                            >  2:4489282/1‑150 (MQ=255)

                                           cTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATc                                                                                                            >  1:4926479/1‑150 (MQ=255)

                                             ttGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATccc                                                                                                          <  1:1936860/150‑1 (MQ=255)

                                             ttGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATccc                                                                                                          <  1:526378/150‑1 (MQ=255)

                                             ttGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATccc                                                                                                          <  1:1937064/150‑1 (MQ=255)

                                             ttGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATccc                                                                                                          <  2:4988788/150‑1 (MQ=255)

                                                tggtgAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGa                                                                                                       <  1:1489015/150‑1 (MQ=255)

                                                    gAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCg                                                                                                   <  1:3010256/150‑1 (MQ=255)

                                                                   ttcttTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGa                                                                                    >  1:1671202/1‑150 (MQ=255)

                                                                    tcttTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGag                                                                                   <  1:1983695/150‑1 (MQ=255)

                                                                    tcttTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGag                                                                                   <  2:4196154/150‑1 (MQ=255)

                                                                         ggATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCt                                                                              >  1:4287739/1‑150 (MQ=255)

                                                                         ggATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCt                                                                              >  2:1096971/1‑150 (MQ=255)

                                                                                ttCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTg                                                                       >  1:1829604/1‑150 (MQ=255)

                                                                                ttCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTg                                                                       >  2:636085/1‑150 (MQ=255)

                                                                                ttCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGACCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTg                                                                       >  1:1258856/1‑150 (MQ=255)

                                                                                 tCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTGTCCTTCAGGAAAACCtt                                                                                                                                            <  2:3118813/80‑1 (MQ=38)

                                                                                   gAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCg                                                                    >  1:4722048/1‑150 (MQ=255)

                                                                                    aGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCgg                                                                   >  2:3571846/1‑150 (MQ=255)

                                                                                      gCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAg                                                                 >  2:1387831/1‑150 (MQ=255)

                                                                                       cGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAgc                                                                <  2:2863029/150‑1 (MQ=255)

                                                                                       cGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAgc                                                                <  2:3013561/150‑1 (MQ=255)

                                                                                        gTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTc                                                                                                                              >  2:3012385/1‑87 (MQ=255)

                                                                                        gTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAgcg                                                               >  1:3062603/1‑150 (MQ=255)

                                                                                          tcttcGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCa                                                             <  1:2298586/150‑1 (MQ=255)

                                                                                           cttcGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCAt                                                            >  2:2101037/1‑150 (MQ=255)

                                                                                           cttcGGGCCGAAAGGGCGCCTGCAGCAGATTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCCGCTCGAGCTGGCGGAGCGCAt                                                            >  2:4674423/1‑150 (MQ=255)

                                                                                            ttcGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATc                                                           >  1:3097038/1‑150 (MQ=255)

                                                                                            ttcGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATc                                                           >  1:4585236/1‑150 (MQ=255)

                                                                                            ttcGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATc                                                           <  1:1748217/150‑1 (MQ=255)

                                                                                               gggCCGAAAGGGCGCCTTCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCcgc                                                        <  2:1736785/150‑1 (MQ=255)

                                                                                               gggCCGAAAGGGCGCCTTCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCcgc                                                        <  2:1736829/150‑1 (MQ=255)

                                                                                               gggCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCcgc                                                        <  1:2054635/150‑1 (MQ=255)

                                                                                               gggCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCcgc                                                        <  2:5077811/150‑1 (MQ=255)

                                                                                                ggCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCcgcg                                                       >  2:4708534/1‑150 (MQ=255)

                                                                                                 gCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGa                                                      >  1:2992888/1‑150 (MQ=255)

                                                                                                     aaaGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAAcc                                                  >  2:218894/1‑150 (MQ=255)

                                                                                                        gggCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACcttc                                               <  2:1439518/150‑1 (MQ=255)

                                                                                                          gcgcCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACcttctt                                             <  1:4545829/150‑1 (MQ=255)

                                                                                                          gcgcCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACcttctt                                             <  1:3119235/150‑1 (MQ=255)

                                                                                                            gcCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCg                                           >  2:692306/1‑150 (MQ=255)

                                                                                                              cTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGAt                                         >  1:2285879/1‑150 (MQ=255)

                                                                                                                 cagcagTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAg                                      >  2:1774401/1‑150 (MQ=255)

                                                                                                                    cagTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGt                                   <  2:3198276/150‑1 (MQ=255)

                                                                                                                     agTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTg                                  <  2:927808/150‑1 (MQ=255)

                                                                                                                     agTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTg                                  <  1:4069329/150‑1 (MQ=255)

                                                                                                                       tttAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGc                                <  2:1675369/150‑1 (MQ=255)

                                                                                                                             gATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTa                          >  2:4357633/1‑150 (MQ=255)

                                                                                                                                caaggcctcAAGGTTGTCCTTCAGGAAAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAgt                       >  1:1035914/7‑150 (MQ=39)

                                                                                                                                cGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAgt                       <  1:4761376/150‑1 (MQ=255)

                                                                                                                                 aaggcctcAAGGTTGTCCTTCAGGAAAACCTTGAGACCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAgtg                      >  1:341414/6‑150 (MQ=39)

                                                                                                                                  gTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAgtgt                     <  1:3821123/150‑1 (MQ=255)

                                                                                                                                     tctcAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCa                  <  2:3462512/150‑1 (MQ=255)

                                                                                                                                       tcAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGt                >  2:1538179/1‑150 (MQ=255)

                                                                                                                                       tcAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGt                >  1:5019924/1‑150 (MQ=255)

                                                                                                                                       tcAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGt                >  2:3675510/1‑150 (MQ=255)

                                                                                                                                       tcAAGGTTGTCCTTCAGGAAAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGt                >  2:3657901/1‑150 (MQ=255)

                                                                                                                                        cAAGGTTTTCCTGTTGGAGAAGGTTGAGGCCTTGCTGTCCGGTCAGCAGGGGCAATCCGTGATTCGTGCGGACGTGATCGAGCAGGTGGAGGTGGCGGAGCGCTTCCGCGAATCCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGtt               >  2:3729435/1‑150 (MQ=255)

                                                                                                                                        cAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGtt               >  2:1378478/1‑150 (MQ=255)

                                                                                                                                         aaGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACct                                                 <  2:190823/115‑1 (MQ=255)

                                                                                                                                         aaGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACct                                                 >  1:190823/1‑115 (MQ=255)

                                                                                                                                          aGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCa             >  1:984083/1‑150 (MQ=255)

                                                                                                                                           ggTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAg            >  2:4657260/1‑150 (MQ=255)

                                                                                                                                                   tGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAGc    <  1:2601544/150‑1 (MQ=255)

                                                                                                                                                   tGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAGc    >  1:2211279/1‑150 (MQ=255)

                                                                                                                                                   tGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAGc    >  2:3276953/1‑150 (MQ=255)

                                                                                                                                                    gAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAGcc   <  2:2501210/150‑1 (MQ=255)

                                                                                                                                                     aaGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAGccc  <  2:1587912/150‑1 (MQ=255)

ACGTGTACAGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAGCCC  >  NC_002947/3008290‑3008588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`