BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,071,335	G→A	6.0%	E153E (GAG→GAA)	aldB‑II →	aldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,071,335	0	G	A	6.0%	545.5 / 14.4	165	E153E (GAG→GAA)	aldB‑II	aldehyde dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (75/80); new base A (6/4); total (81/84)
Fisher's exact test for biased strand distribution p-value = 5.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 167 total aligned reads displayed.

TGGGACAACGGCAAGGCCATACGCGAAACCTTGAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGACGCCGCTGTCGATTACCGTCTTTGCCGAACT  >  NC_002947/3071186‑3071484

tGGGACAACGGCAAGGCCATACGCGAAACCTTGAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAg                                                                                                                                                       >  1:3204149/1‑150 (MQ=255)

      aaCGGCAAGGCCATACGCGAAACCTTGAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTg                                                                                                                                                 >  1:793052/1‑150 (MQ=255)

       aCGGCAAGGCCATACGCGAAACCTTGAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTgg                                                                                                                                                <  1:302964/150‑1 (MQ=255)

         ggCATGGCCATACGCGAAACCTTGAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGgt                                                                                                                                              <  1:1214804/150‑1 (MQ=255)

         ggCAAGGCCATACGCGAAACCTTGAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGgt                                                                                                                                              >  2:465554/1‑150 (MQ=255)

           cAAGGCCATACGCGAAACCTTGAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCCGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGgtgt                                                                                                                                            >  1:285691/1‑150 (MQ=255)

            aaGGCCATACGCGAAACCTTGAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGtgtg                                                                                                                                           >  2:4919475/1‑150 (MQ=255)

              ggCCATACGCGAAACCTTGAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTgtggt                                                                                                                                         <  1:2986702/150‑1 (MQ=255)

                ccATACGCGAAACCTTGAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGtggtgg                                                                                                                                       <  2:2860609/150‑1 (MQ=255)

                 cATACGCGAAACCTTGAATGCCGACGTGACGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCTTAGGCGAGATCAACGAAGGCACCTTGGCTTATCACATTCACTAGCCGCAGGGTGTGGTggg                                                                                                                                      >  1:5098029/1‑150 (MQ=255)

                  aTACGCGAAACCTTGAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTgggg                                                                                                                                     >  1:529214/1‑150 (MQ=255)

                   tACGCGAAACCTTGACTGCCGACGTGCCGCGGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGc                                                                                                                                    <  2:217205/150‑1 (MQ=255)

                   tACGCGAAACCTTGAATGCCGACGTGACGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGc                                                                                                                                    >  1:2136296/1‑150 (MQ=255)

                     cgcgAAACCTTGAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAg                                                                                                                                  >  1:2192823/1‑150 (MQ=255)

                      gcgAAACCTTGAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGa                                                                                                                                 <  1:918828/150‑1 (MQ=255)

                            ccTTGAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATcc                                                                                                                           >  1:1365150/1‑150 (MQ=255)

                             cTTGAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATccc                                                                                                                          <  1:961625/150‑1 (MQ=255)

                             cTTGAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATccc                                                                                                                          >  2:4642163/1‑150 (MQ=255)

                              ttGAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCg                                                                                                                         >  2:3130106/1‑150 (MQ=255)

                               tGAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGt                                                                                                                        <  1:1900504/150‑1 (MQ=255)

                               tGAATGCCGACGTGCCGCTGGCAGCGGACCACGTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGt                                                                                                                        <  2:627589/150‑1 (MQ=255)

                                gAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTg                                                                                                                       <  2:2831434/150‑1 (MQ=255)

                                gAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTg                                                                                                                       <  2:2831449/150‑1 (MQ=255)

                                 aaTGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTgg                                                                                                                      <  2:595348/150‑1 (MQ=255)

                                    gCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAAc                                                                                                                   >  1:1667810/1‑150 (MQ=255)

                                      cGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACtt                                                                                                                 >  1:201577/1‑150 (MQ=255)

                                       gACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTc                                                                                                                <  1:4990131/150‑1 (MQ=255)

                                       gACGTGCCGCTGACAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTc                                                                                                                <  1:297607/150‑1 (MQ=255)

                                          gTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCg                                                                                                             <  2:2622305/150‑1 (MQ=255)

                                              cGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCgctgc                                                                                                         <  1:4446105/150‑1 (MQ=255)

                                               gCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCgctgct                                                                                                        >  1:4896505/1‑150 (MQ=255)

                                                   gCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATg                                                                                                    <  2:1226841/150‑1 (MQ=255)

                                                   gCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATg                                                                                                    >  2:2947750/1‑150 (MQ=255)

                                                    ctattgACCACTTCCGTTATTTTGCCGGTGCAATCCGTGCGCAGGAAGGTGGTATTTCGGAAATCGACCACGATACGGTTGCGTATCATTTCCACGAACCGCTCGGTGTTGTCGGCCAGATCATCCCCTGGAACTTCCCGCTGCTGATgg                                                                                                   <  1:582383/145‑1 (MQ=2)

                                                    cAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATgg                                                                                                   <  1:3535131/150‑1 (MQ=255)

                                                     aGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGgc                                                                                                  >  1:1006543/1‑150 (MQ=255)

                                                      attgACCACTTCCGTTATTTTGCCGGTGCAATCCGTGCGCAGGAAGGTGGTATTTCGGAAATCGACCACGATACGGTTGCGTATCATTTCCACGAACCGCTCGGTGTTGTCGGCCAGATCATCCCCTGGAACTTCCCGCTGCTGATGgca                                                                                                 <  2:1134277/147‑2 (MQ=2)

                                                      attgACCACGTCCGTTATTTTGCCGGTGCAATCCGTGCGCAGGAAGGTGGTATTTCGGAAATCGACCACGATACGGTTGCGTATCATTTCCACGAACCGCTCGGTATTGTCGGCCAGATCATCCCCTGGAACTTCCCGCTGCTGATGgca                                                                                                 <  2:1518529/147‑2 (MQ=2)

                                                       ttgACCACTTCCGTTATTTTGCCGGTGCAATCCGTGCGCAGGAAGGTGGTATTTCGGAAATCGACCACGATACGGTTGCGTATCATTTCCACGAACCGCTCGGTGTTGTCGGCCAGATCATCCCCTGGAACTTCCCGCTGCTGATGgcag                                                                                                >  2:436500/3‑148 (MQ=2)

                                                       ttgACCACTTCCGTTATTTTGCCGGTGCAATCCGTGCGCAGGAAGGTGGTATTTCGGAAATCGACCACGATACGGTTGCGTATCATTTCCACGAACCGCTCGGTGTTGTCGGCCAGATCATCCCCTGGAACTTCCCGCTGCTGATGgcag                                                                                                >  2:436438/3‑148 (MQ=2)

                                                        ggACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGgccgc                                                                                               >  1:252292/1‑150 (MQ=255)

                                                          aCCACTTCCGTTATTTTGCCGGTGCAATCCGTGCGCAGGAAGGTGGTATTTCGGAAATCGACCACGATACGGTTGCGTATCATTTCCACGAACCGCTCGGTGTTGTCGGCCAGATCATCCCCTGGAACTTCCCGCTGCTGATGgcagtat                                                                                             >  2:3743023/1‑145 (MQ=2)

                                                          aCCACTTCCGTTATTTTGCCGGTGCAATCCGTGCGCAGGAAGGTGGTATTTCGGAAATCGACCACGATACGGTTGCGTATCATTTCCACGAACCGCTCGGTGTTGTCGGCCAGATCATCCCCTGGAACTTCCCGCTGCTGATGgcagtat                                                                                             >  2:3743213/1‑145 (MQ=2)

                                                          aCCACTTCCGTTATTTTGCCGGTGCAATCCGTGCGCAGGAAGGTGGTATTTCGGAAATCGACCACGATACGGTTGCGTATCATTTCCACGAACCGCTCGGTGTTGTCGGCCAGATCATCCCCTGGAACTTCCCGCTGCTGATGgcagtat                                                                                             >  2:3746624/1‑145 (MQ=2)

                                                          aCCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCt                                                                                             <  2:1664538/150‑1 (MQ=255)

                                                            cACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTgg                                                                                           >  2:2997713/1‑150 (MQ=255)

                                                              cTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGaa                                                                                         <  1:2126780/150‑1 (MQ=255)

                                                              cTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGaa                                                                                         >  1:4639248/1‑150 (MQ=255)

                                                              cTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGaa                                                                                         <  1:3731267/150‑1 (MQ=255)

                                                              cTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGACGGCCGCCTGGaa                                                                                         <  1:1524252/150‑1 (MQ=255)

                                                                   gCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCg                                                                                    >  2:5071445/1‑150 (MQ=255)

                                                                     tATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGcc                                                                                  <  1:2111512/150‑1 (MQ=255)

                                                                               gTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTgg                                                                        >  2:4707955/1‑150 (MQ=255)

                                                                               gTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTgg                                                                        >  2:4773445/1‑150 (MQ=255)

                                                                                ttGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGc                                                                       <  2:664311/150‑1 (MQ=255)

                                                                                   cATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGc                                                                    <  2:3579391/150‑1 (MQ=255)

                                                                                    ctCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATGCCCGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCt                                                                   <  2:2179688/149‑1 (MQ=255)

                                                                                     tCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTg                                                                  >  2:3818139/1‑150 (MQ=255)

                                                                                     tCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTg                                                                  >  1:2399040/1‑150 (MQ=255)

                                                                                      ccGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTgg                                                                 >  2:4520089/1‑150 (MQ=255)

                                                                                      ccGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTgg                                                                 >  2:1943278/1‑150 (MQ=255)

                                                                                           cccAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACt                                                            <  1:2730686/150‑1 (MQ=255)

                                                                                               ggaggGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCgt                                                        <  2:3888171/150‑1 (MQ=255)

                                                                                                gaggGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCgtg                                                       <  2:432536/150‑1 (MQ=255)

                                                                                                gaggGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCgtg                                                       <  1:1698587/150‑1 (MQ=255)

                                                                                                gaggGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCgtg                                                       <  2:252292/150‑1 (MQ=255)

                                                                                                gaggGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCgtg                                                       <  2:4161891/150‑1 (MQ=255)

                                                                                                gaggGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCgtg                                                       <  2:4161607/150‑1 (MQ=255)

                                                                                                  gggTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCgtggt                                                     <  2:793052/150‑1 (MQ=255)

                                                                                                    gTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGc                                                   <  2:1109242/150‑1 (MQ=255)

                                                                                                        gttAGGCGATATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTTCTCaa                                               <  2:298103/148‑1 (MQ=255)

                                                                                                        cGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCaa                                               <  1:1366086/150‑1 (MQ=255)

                                                                                                              cGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCcgc                                         <  2:3204149/150‑1 (MQ=255)

                                                                                                                agaTCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCgg                                       >  2:1906646/1‑150 (MQ=255)

                                                                                                                  aTCAACGAAGGCACCGTGGCTTATCACAGTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAg                                     <  2:3703825/150‑1 (MQ=255)

                                                                                                                   tCAACGAAGTCACCGTGGCTTATCACATTCACGAGGCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGc                                    <  2:2426346/150‑1 (MQ=255)

                                                                                                                    cAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCa                                   <  1:4207793/150‑1 (MQ=255)

                                                                                                                     aaCGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAg                                  >  2:4595912/1‑150 (MQ=255)

                                                                                                                     aaCGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAg                                  >  1:435492/1‑150 (MQ=255)

                                                                                                                        gAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGAcg                               >  1:1021158/1‑150 (MQ=255)

                                                                                                                        gAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGAcg                               >  1:6138/1‑150 (MQ=255)

                                                                                                                         aaGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGAcgc                              <  1:4541586/150‑1 (MQ=255)

                                                                                                                           ggCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGAcgccg                            >  1:2318936/1‑150 (MQ=255)

                                                                                                                              aCCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGTGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGACGCCGCTg                         >  2:1617581/1‑150 (MQ=255)

                                                                                                                              aCCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGACGCCGCTg                         >  2:3607441/1‑150 (MQ=255)

                                                                                                                                  tGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGACGCCGCTGTCGa                     >  2:5013518/1‑150 (MQ=255)

                                                                                                                                    gCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGACGCCGCTGTCGAtt                   <  2:2896552/150‑1 (MQ=255)

                                                                                                                                     cTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGACGCCGCTGTCGATTa                  <  1:1961016/150‑1 (MQ=255)

                                                                                                                                     cTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGACGCCGCTGTCGATTa                  <  1:3036549/150‑1 (MQ=255)

                                                                                                                                      ttATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTg                                                                  <  1:3336351/101‑1 (MQ=255)

                                                                                                                                      ttATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGACGCCGCTGTCGATTAc                 >  1:846240/1‑150 (MQ=255)

                                                                                                                                        aTCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGACGCCGCTGTCGATTACCg               >  1:2690756/1‑150 (MQ=255)

                                                                                                                                         tCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGACGCCGCTGTCGATTACCGt              >  2:3378370/1‑150 (MQ=255)

                                                                                                                                          cacaTTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGACGCCGCTGTCGATTACCGTc             >  2:4000865/1‑150 (MQ=255)

                                                                                                                                           acaTTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGACGCCGCTGTCGATTACCGTCt            >  1:4639763/1‑150 (MQ=255)

                                                                                                                                             aTTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGACGCCGCTGTCGATTACCGTCttt          <  1:2911649/150‑1 (MQ=255)

                                                                                                                                             aTTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGACGCCGCTGTCGATTACCGTCttt          <  1:4479008/150‑1 (MQ=255)

                                                                                                                                              ttCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGACGCCGCTGTCGATTACCGTCTTTg         >  1:3995781/1‑150 (MQ=255)

                                                                                                                                                cACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGACGCCGCTGTCGATTACCGTCTTTGcc       <  2:211478/150‑1 (MQ=255)

                                                                                                                                                cACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGACGCCGCTGTCGATTACCGTCTTTGcc       <  2:6138/150‑1 (MQ=255)

TGGGACAACGGCAAGGCCATACGCGAAACCTTGAATGCCGACGTGCCGCTGGCAGCGGACCACTTCCGCTATTTTGCCGGTTGCATCCGCGCCCAGGAGGGTGGCGTAGGCGAGATCAACGAAGGCACCGTGGCTTATCACATTCACGAGCCGCTGGGTGTGGTGGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTCGCCCCGGCCTTGGCCGCTGGCAACTGCGTGGTGCTCAAGCCCGCGGAGCAGACGCCGCTGTCGATTACCGTCTTTGCCGAACT  >  NC_002947/3071186‑3071484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`