Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,072,461 T→C 5.3% intergenic (+64/‑290) aldB‑II → / → pqqD‑II aldehyde dehydrogenase/coenzyme PQQ synthesis protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,072,4610TC 5.3% 494.8 / 9.6 151intergenic (+64/‑290)aldB‑II/pqqD‑IIaldehyde dehydrogenase/coenzyme PQQ synthesis protein D
Reads supporting (aligned to +/- strand):  ref base T (74/69);  new base C (2/6);  total (76/75)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

Only 100 of 153 total aligned reads displayed.

AGACCCACAAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCCTCCGGCAC  >  NC_002947/3072312‑3072609
                                                                                                                                                     |                                                                                                                                                    
agaCCCACAAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGt                                                                                                                                                      <  2:2300761/150‑1 (MQ=255)
  aCCCACAAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTga                                                                                                                                                    >  1:2622888/1‑150 (MQ=255)
    ccACAAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTgaag                                                                                                                                                  >  1:277913/1‑150 (MQ=255)
    ccACAAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGCTTCACCGCAGGTTCCAGAAACGGTgaag                                                                                                                                                  >  1:2720883/1‑150 (MQ=255)
     cacaAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTgaaga                                                                                                                                                 >  2:4829135/1‑150 (MQ=255)
      acaAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTgaagaa                                                                                                                                                >  1:3424767/1‑150 (MQ=255)
      acaAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTgaagaa                                                                                                                                                >  1:4060980/1‑150 (MQ=255)
       caAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAAc                                                                                                                                               <  2:1773714/150‑1 (MQ=255)
         aGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCt                                                                                                                                             <  2:2800137/150‑1 (MQ=255)
         aGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCt                                                                                                                                             <  1:2032175/150‑1 (MQ=255)
          gatgatGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCtg                                                                                                                                            >  1:2134545/1‑150 (MQ=255)
           atgatgCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCtgt                                                                                                                                           <  1:1540408/150‑1 (MQ=255)
                gCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTggggg                                                                                                                                      >  1:4290894/1‑150 (MQ=255)
                 cTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCTTCACCGGTTCTGGAACCAGCGGTGAACCTGTGGgagc                                                                                                                                     <  1:3356773/150‑1 (MQ=18)
                  ttGACCATTATCAGCCGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGgggcg                                                                                                                                    <  1:409968/150‑1 (MQ=255)
                   tGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGgggcgg                                                                                                                                   <  1:2934946/150‑1 (MQ=255)
                   tGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCGTCGCGGGCTTGCCCGCCCCCACAGGTTCTTCACCGGTTCTGGAACCAGCGGTGAACCTGTGGgagcgg                                                                                                                                   <  1:1265246/150‑1 (MQ=18)
                   tGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCGTCGCGGGCTTGCCCGCCCCCACAGGTTCTTCACCGGTTCTGGAACCAGCGGTGAACCTGTGGgagcgg                                                                                                                                   <  1:1265245/150‑1 (MQ=18)
                     aCCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGgggcgggc                                                                                                                                 <  2:633413/150‑1 (MQ=255)
                       ccttATCAGCAGACCCAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTTGCGGGCTCGCCCGCCCCCACAGGTTCTTCACCGGTTCTGGAACCAGCGGTGAACCTGTGGgagcgggcaa                                                                                                                               <  1:3837835/148‑3 (MQ=17)
                       cATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGa                                                                                                                               <  2:3031481/150‑1 (MQ=255)
                       cATTATCAGCAGACCAAGAACCAGCTGGTGAGCTACGACATCAATCCGCGGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGACCCGGTGAAGAACCTGTGGGGGCGGGCGa                                                                                                                               <  2:2576143/150‑1 (MQ=255)
                        aTTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAg                                                                                                                              >  2:1798304/1‑150 (MQ=255)
                         ttATCAGCAGACCAAGACCCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCCCCCACAGGTTCTTCACCGGTTCTGGAACCAGCGGTGAACCTGTGGGAGCGGGCAAGc                                                                                                                             <  1:4621322/150‑1 (MQ=17)
                            tCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCg                                                                                                                          >  1:4138621/1‑150 (MQ=255)
                             cagcagACCACGACCCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTGGCCCGCTCCCCCAGGTTCACCGCTGGTTCCAGACCCGGTGAAGAACCTGGGGGGGCGGGCGAGCCCGc                                                                                                                         <  1:1063920/150‑1 (MQ=18)
                               gcagACCAAGACCCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCat                                                                                                                       <  1:3034295/150‑2 (MQ=255)
                                   aCCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAgg                                                                                                                   >  2:2586339/1‑150 (MQ=255)
                                   aCCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAgg                                                                                                                   <  1:3435082/150‑1 (MQ=255)
                                    ccAAGAACCTGCTGGTGAGCTCCGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAggg                                                                                                                  <  1:193810/150‑1 (MQ=255)
                                     cAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGc                                                                                                                 >  2:1714202/1‑150 (MQ=255)
                                     cAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGc                                                                                                                 >  1:365134/1‑150 (MQ=255)
                                     cAAGAACCTGCTGGTGAGCTACGACATCAATCCGATGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGc                                                                                                                 >  2:656484/1‑150 (MQ=255)
                                      aaGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGcc                                                                                                                >  2:3814111/1‑150 (MQ=255)
                                       aGAACCTGCTGGTGAGCTACGACATCACTCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCg                                                                                                               <  2:4974260/150‑1 (MQ=255)
                                        gAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCgg                                                                                                              <  1:4827218/150‑1 (MQ=255)
                                           ccTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGcac                                                                                                           >  2:2211361/1‑150 (MQ=255)
                                             tgctgGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCAcat                                                                                                         >  1:1071410/1‑150 (MQ=255)
                                                   tGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATgg                                                                                                   <  1:2697264/150‑1 (MQ=255)
                                                    gAGCTCCGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCCAAAGGGCCGGCACATGCCTGGc                                                                                                  >  2:3498692/1‑150 (MQ=255)
                                                    gAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGc                                                                                                  <  2:1316881/150‑1 (MQ=255)
                                                      gCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCTCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCt                                                                                                <  1:1781754/150‑1 (MQ=255)
                                                       cTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTa                                                                                               >  1:4906206/1‑150 (MQ=255)
                                                        tACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCTAGCCCGCCAAAGGGCCGGCACAGGCCTGGCCTAc                                                                                              >  1:2568730/1‑150 (MQ=255)
                                                        tACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTAc                                                                                              <  2:4228562/150‑1 (MQ=255)
                                                         aCGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTAcc                                                                                             >  1:1046927/1‑150 (MQ=255)
                                                          cGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTAccc                                                                                            <  1:3710845/150‑1 (MQ=255)
                                                          cGACATCAATCCGCGGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCCGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTAccc                                                                                            <  1:1871387/150‑1 (MQ=255)
                                                              aTCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTAc                                                                                        >  1:2509858/1‑150 (MQ=255)
                                                                 aaTCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCaa                                                                                     <  2:368036/150‑1 (MQ=255)
                                                                 aaTCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCaa                                                                                     <  1:3024437/150‑1 (MQ=255)
                                                                 aaTCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACAAGCCTGGCCTACCCGTACCaa                                                                                     >  1:2691884/1‑150 (MQ=255)
                                                                  aTCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAAc                                                                                    >  2:2024274/1‑150 (MQ=255)
                                                                   tCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACg                                                                                   >  2:71152/1‑150 (MQ=255)
                                                                         gggCTTCGTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCAcccc                                                                             >  1:768309/1‑150 (MQ=255)
                                                                            cttcttCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCa                                                                          >  1:3515556/1‑150 (MQ=255)
                                                                            cttcttCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCa                                                                          >  2:2401908/1‑150 (MQ=255)
                                                                             ttcttcTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCaa                                                                         <  1:1126421/150‑1 (MQ=255)
                                                                              tcttctAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAg                                                                        >  1:3541224/1‑150 (MQ=255)
                                                                                 tctAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTc                                                                     <  2:2258075/150‑1 (MQ=255)
                                                                                 tctAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTc                                                                     <  1:436990/150‑1 (MQ=255)
                                                                                 tctAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTc                                                                     <  2:1800477/150‑1 (MQ=255)
                                                                                  ctAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCg                                                                    >  2:4278691/1‑150 (MQ=255)
                                                                                  ctAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCg                                                                    <  1:2213873/150‑1 (MQ=255)
                                                                                    aaaCCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGtt                                                                  >  1:3773075/1‑150 (MQ=255)
                                                                                    aaaCCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGtt                                                                  >  1:708514/1‑150 (MQ=255)
                                                                                       ccGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCa                                                               <  2:836560/150‑1 (MQ=255)
                                                                                         gcgTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGTACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCaaa                                                             <  2:771011/150‑1 (MQ=255)
                                                                                         gcgTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCaaa                                                             <  2:770774/150‑1 (MQ=255)
                                                                                            tCGCGGCCTTCGCTGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTa                                                          <  1:3020756/150‑1 (MQ=255)
                                                                                            tCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTa                                                          <  1:3087973/150‑1 (MQ=255)
                                                                                                 gCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCAtt                                                     <  2:2624754/150‑1 (MQ=255)
                                                                                                  cttttgcgGGCTCGCCCGCCCCCACAGGTTCTTCACCGGTTCTGGAACCAGCGGTGAACCTGTGGGAGCGGGCAAGCCCGCGAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTc                                                    >  2:2692296/6‑150 (MQ=14)
                                                                                                   cTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCg                                                   <  1:1798304/150‑1 (MQ=255)
                                                                                                    ttCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCgg                                                  <  1:1333595/150‑1 (MQ=255)
                                                                                                     tCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGt                                                 <  1:135892/150‑1 (MQ=255)
                                                                                                     tCGCGGGCGTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGt                                                 <  2:3929907/150‑1 (MQ=255)
                                                                                                     gagcgGGCAAGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGt                                                 >  1:1420469/3‑150 (MQ=255)
                                                                                                      cgcgGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTc                                                <  2:472346/150‑1 (MQ=255)
                                                                                                        cgGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCa                                              <  2:1444122/150‑1 (MQ=255)
                                                                                                        cgGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCa                                              <  2:1444471/150‑1 (MQ=255)
                                                                                                              tGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGc                                        <  1:4846759/150‑1 (MQ=255)
                                                                                                                cccGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGAATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGccc                                      <  2:206413/150‑1 (MQ=255)
                                                                                                                 ccGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGcccc                                     >  2:1208153/1‑150 (MQ=255)
                                                                                                                 ccGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGcccc                                     >  1:2566525/1‑150 (MQ=255)
                                                                                                                       ccACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCa                               >  2:3328467/1‑150 (MQ=255)
                                                                                                                       ccACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCa                               >  2:4093618/1‑150 (MQ=255)
                                                                                                                        cacaGGTTCACCGCAGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCAt                              >  1:2491386/1‑150 (MQ=255)
                                                                                                                            ggTTCACCGCTGGTTCCAGAACCTGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAATAGGGCCGGCACATTCATGGCCTACCCTTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAAt                          <  2:2720883/150‑1 (MQ=255)
                                                                                                                             gTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATg                         >  1:4476828/1‑150 (MQ=255)
                                                                                                                               tCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCt                       <  1:2398955/150‑1 (MQ=255)
                                                                                                                                 aCCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTc                     >  2:557107/1‑150 (MQ=255)
                                                                                                                                 aCCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTc                     >  1:1956568/1‑150 (MQ=255)
                                                                                                                                           ccAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATAGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAAtc           <  2:3077267/150‑1 (MQ=255)
                                                                                                                                              gAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAAtcctc        <  2:277913/150‑1 (MQ=255)
                                                                                                                                              gAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAAtcctc        >  1:3649542/1‑150 (MQ=255)
                                                                                                                                              gAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAAtcctc        <  1:4829135/150‑1 (MQ=255)
                                                                                                                                                aCCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCCTCCg      <  1:4469421/150‑1 (MQ=255)
                                                                                                                                                 ccGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCCTCCgg     <  2:365134/150‑1 (MQ=255)
                                                                                                                                                    gTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCAACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCCTCCGGcac  >  2:4221102/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGACCCACAAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCCTCCGGCAC  >  NC_002947/3072312‑3072609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑