Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,455,445 A→G 5.4% intergenic (‑34/+79) PP_3069 ← / ← ppiC‑I outer membrane autotransporter/peptidyl‑prolyl cis‑trans isomerase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,455,4450AG 5.4% 465.5 / 10.0 149intergenic (‑34/+79)PP_3069/ppiC‑Iouter membrane autotransporter/peptidyl‑prolyl cis‑trans isomerase C
Reads supporting (aligned to +/- strand):  ref base A (78/62);  new base G (2/6);  total (81/68)
Fisher's exact test for biased strand distribution p-value = 1.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

Only 100 of 153 total aligned reads displayed.

TTACCCGGGCCGGGTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCGTGTACCACAC  >  NC_002947/3455296‑3455591
                                                                                                                                                     |                                                                                                                                                  
ttACCCGGGCCGGGTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGca                                                                                                                                                    >  1:3448759/1‑150 (MQ=255)
  aCCCGGGCCGGGTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAACCCTGTGCACCACGCGTGGTGcgca                                                                                                                                                  <  2:877849/150‑1 (MQ=255)
  aCCCGGGCCGGGTGAGAAGGGACACGCCGCGTGTGCAGTGGGCGCGGCCATGCCCCGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAACACCTCCATTCACCAGGGAAACCTGTGCACCACGCGTGGTGcgca                                                                                                                                                  <  2:3704705/150‑1 (MQ=255)
   cccGGGCCGGGTGAGAAGGTACAAGCCGCGTGTGCAGTGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAg                                                                                                                                                 >  2:534315/1‑150 (MQ=255)
   cccGGGCCGGGTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCGCAg                                                                                                                                                 <  2:1179080/150‑1 (MQ=255)
   cccGGGCCGGGTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAg                                                                                                                                                 <  1:2379634/150‑1 (MQ=255)
    ccgggccgggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCCCAgg                                                                                                                                                >  1:1297045/1‑150 (MQ=255)
    ccgggccgggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAgg                                                                                                                                                <  1:4296323/150‑1 (MQ=255)
       ggccgggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGtt                                                                                                                                             >  2:4286197/1‑150 (MQ=255)
        gccgggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTa                                                                                                                                            >  1:4083978/1‑150 (MQ=255)
          cgggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAACCCTGTGCACCACGCGTGGTGCGCAGGTTTccc                                                                                                                                          <  1:1694116/150‑1 (MQ=255)
          cgggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTAcc                                                                                                                                          >  1:2927649/1‑150 (MQ=255)
           gggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCg                                                                                                                                         <  1:3849408/150‑1 (MQ=255)
            ggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGTAACCCTGTGCACCACGCGTGGTGCGCAGGTTTccctg                                                                                                                                        <  1:5081519/150‑3 (MQ=38)
            ggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGTAACCCTGTGCACCACGCGTGGTGCGCAGGTTTccctg                                                                                                                                        <  1:5081520/150‑3 (MQ=38)
              tGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCCGGTAACCCTGTGCACCACGCGTGGTGCGCAGGTTTCCCtggt                                                                                                                                      <  2:3727092/150‑2 (MQ=38)
               gagaAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGt                                                                                                                                     <  2:3406876/150‑1 (MQ=255)
                agaAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGtt                                                                                                                                    <  2:2533350/150‑1 (MQ=255)
                 gaAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTg                                                                                                                                   >  1:3218356/1‑150 (MQ=255)
                 gaAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTg                                                                                                                                   >  1:2030588/1‑150 (MQ=255)
                 gaAGGTAAAAGCCTCGTGTGCAGCGGGCGAGGACATGCCCAGCAGCGGGAGCGAACCCAGGTACAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTg                                                                                                                                   >  2:2787156/1‑150 (MQ=255)
                    ggTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGtat                                                                                                                                <  2:2297626/150‑1 (MQ=255)
                     gTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGtata                                                                                                                               >  1:732342/1‑150 (MQ=255)
                     gTACAAGCCGCGTGTGCAGCGGGCGAGGCCAGGCCCAGCAGCGGGAGCGACCCCAGGGTCAGCAGCAGGCGTAGACGCATCAGGAAAACCTCCAGTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGtata                                                                                                                               <  2:1719296/150‑1 (MQ=255)
                      tACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATag                                                                                                                              >  1:4890452/1‑150 (MQ=255)
                       aCAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATaga                                                                                                                             <  2:4731496/150‑1 (MQ=255)
                       aCAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATaga                                                                                                                             <  1:2084505/150‑1 (MQ=255)
                        ccaGCCGCGTGTACAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATagag                                                                                                                            <  2:4151599/148‑1 (MQ=255)
                         aaGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTTTATAgagg                                                                                                                           >  1:3754464/1‑150 (MQ=255)
                         aaGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGta                                                                                                                                 <  2:2138897/144‑1 (MQ=255)
                         aaGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAgagg                                                                                                                           >  1:3591079/1‑150 (MQ=255)
                          aGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAgagga                                                                                                                          <  1:541775/150‑1 (MQ=255)
                          aGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAgagga                                                                                                                          <  1:541826/150‑1 (MQ=255)
                               cgTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCg                                                                                                                     >  1:3850823/1‑150 (MQ=255)
                                 tgtgCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTc                                                                                                                   <  2:3460528/150‑1 (MQ=255)
                                      aGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGATGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGAc                                                                                                              >  2:3803916/1‑150 (MQ=255)
                                      aGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGAc                                                                                                              >  2:2517937/1‑150 (MQ=255)
                                        cGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACca                                                                                                            <  2:2614280/150‑1 (MQ=255)
                                             gAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTCCCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACt                                                                                                       >  1:4697348/1‑150 (MQ=255)
                                               ggCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTgg                                                                                                     <  2:3191883/150‑1 (MQ=255)
                                                gCCATGCCCCGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGc                                                                                                    <  2:4941097/150‑1 (MQ=255)
                                                gCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGc                                                                                                    <  2:4940875/150‑1 (MQ=255)
                                                  cATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCTTTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCaa                                                                                                  <  2:4772180/150‑1 (MQ=255)
                                                   aTGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCaaa                                                                                                 >  2:2951094/1‑150 (MQ=255)
                                                    tGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCaaaa                                                                                                <  1:966408/150‑1 (MQ=255)
                                                     gCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCaaaat                                                                                               >  2:90858/1‑149 (MQ=255)
                                                      cccAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTCTAGAGGAGTGCGTCACGACCACAACTGGCAAAAAt                                                                                              >  2:3132247/1‑150 (MQ=255)
                                                         agcagcGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTc                                                                                           <  2:2731400/150‑1 (MQ=255)
                                                         agcagcGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTc                                                                                           <  1:2189114/150‑1 (MQ=255)
                                                              cGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTg                                                                                      >  1:812910/1‑150 (MQ=255)
                                                                ggAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGc                                                                                    <  2:453030/150‑1 (MQ=255)
                                                                         ccAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCtc                                                                           >  2:4499598/1‑149 (MQ=255)
                                                                         ccAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTa                                                                           >  2:4499664/1‑150 (MQ=255)
                                                                         ccAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCAGTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTa                                                                           <  1:4442166/150‑1 (MQ=255)
                                                                             ggTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAgcgc                                                                       <  1:5048001/150‑1 (MQ=255)
                                                                               tCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGt                                                                     <  1:223629/150‑1 (MQ=255)
                                                                               tCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGt                                                                     <  1:38369/150‑1 (MQ=255)
                                                                                 aGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTa                                                                   <  1:2242284/150‑1 (MQ=255)
                                                                                 aGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTa                                                                   <  1:2242172/150‑1 (MQ=255)
                                                                                 aGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTa                                                                   <  1:481317/150‑1 (MQ=255)
                                                                                     tCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTcg                                                               >  1:2828056/1‑150 (MQ=255)
                                                                                         gCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGt                                                           >  1:1021813/1‑150 (MQ=255)
                                                                                         gCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGt                                                           >  1:3759179/1‑150 (MQ=255)
                                                                                               aCGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACg                                                     <  2:3178110/150‑1 (MQ=255)
                                                                                                 gCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGtt                                                   <  1:646313/150‑1 (MQ=255)
                                                                                                 gCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGtt                                                   >  2:4323993/1‑150 (MQ=255)
                                                                                                  cATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGttt                                                  <  2:3614596/150‑1 (MQ=255)
                                                                                                   aTCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTg                                                 >  2:562323/1‑150 (MQ=255)
                                                                                                   aTCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTg                                                 <  2:3850823/150‑1 (MQ=255)
                                                                                                   aTCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTg                                                 <  1:4184136/150‑1 (MQ=255)
                                                                                                      aGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCAc                                              >  1:2741019/1‑150 (MQ=255)
                                                                                                        gAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCa                                            >  2:1285094/1‑150 (MQ=255)
                                                                                                        gAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCa                                            >  2:3308323/1‑150 (MQ=255)
                                                                                                           aaCCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAggt                                         <  1:1149969/150‑1 (MQ=255)
                                                                                                            aCCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAggtg                                        >  1:4171846/1‑150 (MQ=255)
                                                                                                              ctacaatCAACAGGGAAACCAGCGCACCACGCCTGGTGCACAGGGTTACCGGGGTTGTATACAGGAGTGCGTAAACACCACAACTGGCAAAAATGTAAGGTGGCAAAGGGCAAGCGCGTTAGTAGCGGTAGAACGTTTGAAACAggtggt                                      >  2:4372345/7‑150 (MQ=255)
                                                                                                              cTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAggtggt                                      >  1:3581902/1‑150 (MQ=255)
                                                                                                              cTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAggtggt                                      >  1:1581745/1‑150 (MQ=255)
                                                                                                              cTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAggtggt                                      >  1:3041407/1‑150 (MQ=255)
                                                                                                               tCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTa                                     >  1:2664163/1‑150 (MQ=255)
                                                                                                               tCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTa                                     >  2:4450150/1‑150 (MQ=255)
                                                                                                               tCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTa                                     >  2:501511/1‑150 (MQ=255)
                                                                                                                   ttCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCg                                 >  2:503474/1‑150 (MQ=255)
                                                                                                                         aGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTg                           >  2:3493804/1‑150 (MQ=255)
                                                                                                                         aGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTg                           >  1:441211/1‑150 (MQ=255)
                                                                                                                            gAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGctc                        <  1:3487982/150‑1 (MQ=255)
                                                                                                                             aaaCCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGctct                       <  1:3389861/150‑1 (MQ=255)
                                                                                                                                ccTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGa                    <  2:844048/150‑1 (MQ=255)
                                                                                                                                 cTGTGCACCACGCGTGGTGCGCAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGAt                   >  1:3622085/1‑150 (MQ=255)
                                                                                                                                 cTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGAt                   <  1:2428310/150‑1 (MQ=255)
                                                                                                                                  tGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATc                  <  2:364262/150‑1 (MQ=255)
                                                                                                                                  tGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATc                  >  1:3199343/1‑150 (MQ=255)
                                                                                                                                   gtgcACCACGCGTGGTGCGCAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCg                 >  1:4361716/3‑150 (MQ=255)
                                                                                                                                     gcACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGc               >  2:2061024/1‑150 (MQ=255)
                                                                                                                                       accacGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGccc             >  1:3566534/1‑150 (MQ=255)
                                                                                                                                       accacGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGccc             >  2:2951626/1‑150 (MQ=255)
                                                                                                                                               tggtgCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCGTGTACca     <  2:1462068/150‑1 (MQ=255)
                                                                                                                                                ggtgCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCGTGTACcac    >  1:1099216/1‑150 (MQ=255)
                                                                                                                                                 gtgCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCGTGTACcaca   <  2:1297045/150‑1 (MQ=255)
                                                                                                                                                  tgCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCGTGTACCacac  <  2:4499925/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TTACCCGGGCCGGGTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGCACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCGTGTACCACAC  >  NC_002947/3455296‑3455591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑