Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 264,158 C→T 13.6% D303D (GAC→GAT)  gabD‑I → succinate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947264,1580CT13.6% 412.0 / 45.9 161D303D (GAC→GAT) gabD‑Isuccinate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (59/80);  new base T (10/12);  total (69/92)
Fisher's exact test for biased strand distribution p-value = 8.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 167 total aligned reads displayed.

CTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGCC  >  NC_002947/264009‑264305
                                                                                                                                                     |                                                                                                                                                   
cTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTacgac                                                                                                                                                     <  2:1605794/150‑1 (MQ=255)
     gCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGtt                                                                                                                                                <  2:1358664/150‑1 (MQ=255)
       ttggcggCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCtttg                                                                                                                                              >  2:2372062/6‑148 (MQ=255)
       ttggcggCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCtttg                                                                                                                                              >  2:4943447/6‑148 (MQ=255)
         ggTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGcc                                                                                                                                            <  2:1850867/150‑1 (MQ=255)
          gcggCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCg                                                                                                                                           >  1:3613554/3‑150 (MQ=255)
            ggCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCgag                                                                                                                                         <  2:3547964/150‑1 (MQ=255)
               aaCGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAg                                                                                                                                      >  1:4113681/1‑150 (MQ=255)
               aaCGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAg                                                                                                                                      >  2:3566310/1‑150 (MQ=255)
               aaCGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAg                                                                                                                                      >  2:4467793/1‑150 (MQ=255)
               aaCGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAg                                                                                                                                      <  2:4916992/150‑1 (MQ=255)
               aaCGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAg                                                                                                                                      >  1:3553991/1‑150 (MQ=255)
               aaCGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAg                                                                                                                                      >  1:1631468/1‑150 (MQ=255)
                 cGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCt                                                                                                                                    <  2:1798581/150‑1 (MQ=255)
                  gcgcctttCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTATGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCtc                                                                                                                                   >  2:443802/7‑149 (MQ=255)
                   ccccGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTgg                                                                                                                                  <  2:4195669/150‑1 (MQ=255)
                   ccccGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTgg                                                                                                                                  <  2:878868/150‑1 (MQ=255)
                    cccGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGc                                                                                                                                 >  1:1420307/1‑150 (MQ=255)
                    cccGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGAATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGc                                                                                                                                 >  1:3483581/1‑150 (MQ=255)
                     cctttCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACCACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCtctcc                                                                                                                                <  2:3876646/147‑5 (MQ=255)
                     ccGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGcc                                                                                                                                >  2:2712089/1‑150 (MQ=255)
                     ccGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGcc                                                                                                                                >  2:1866792/1‑150 (MQ=255)
                           aTCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCa                                                                                                                          <  2:2517855/150‑1 (MQ=255)
                           aTCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCa                                                                                                                          >  1:1294339/1‑150 (MQ=255)
                           aTCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCa                                                                                                                          >  1:1294355/1‑150 (MQ=255)
                             cGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGt                                                                                                                        >  1:4239681/1‑150 (MQ=255)
                             cGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGt                                                                                                                        <  1:2412087/150‑1 (MQ=255)
                                gtTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGc                                                                                                                     <  1:2033860/150‑1 (MQ=255)
                                     acgacgCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGc                                                                                                                <  1:2029958/150‑1 (MQ=255)
                                     acgacgCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGc                                                                                                                <  1:3690630/150‑1 (MQ=255)
                                       gacgCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTg                                                                                                              >  1:4296898/1‑150 (MQ=255)
                                          gCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAg                                                                                                           >  1:2453374/1‑150 (MQ=255)
                                            cGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGAt                                                                                                         <  1:2519124/150‑1 (MQ=255)
                                            cGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGAt                                                                                                         <  1:2519111/150‑1 (MQ=255)
                                            cGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGAt                                                                                                         <  1:749825/150‑1 (MQ=255)
                                             gACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATc                                                                                                        >  1:1627621/1‑150 (MQ=255)
                                              aCCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCg                                                                                                       <  1:5079819/150‑1 (MQ=255)
                                              aCCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCg                                                                                                       <  1:4457006/150‑1 (MQ=255)
                                              aCCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCg                                                                                                       <  1:1033305/150‑1 (MQ=255)
                                                   gACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAAc                                                                                                  >  1:3948916/1‑150 (MQ=255)
                                                    aCAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACg                                                                                                 <  1:1593644/150‑1 (MQ=255)
                                                     cAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGACAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACgg                                                                                                >  2:2825377/1‑150 (MQ=255)
                                                      aaGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACggt                                                                                               >  1:3511453/1‑149 (MQ=255)
                                                      aaGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGc                                                                                               <  2:867351/150‑1 (MQ=255)
                                                        ggCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCt                                                                                             <  2:1793626/150‑1 (MQ=255)
                                                        ggCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCt                                                                                             >  2:1111484/1‑150 (MQ=255)
                                                        ggCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCt                                                                                             <  1:5033253/150‑1 (MQ=255)
                                                            gTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGgaa                                                                                         <  1:436248/150‑1 (MQ=255)
                                                             tCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGgaag                                                                                        >  2:1489979/1‑150 (MQ=255)
                                                              cGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGgaaga                                                                                       <  1:2896935/150‑1 (MQ=255)
                                                               gAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGgaagaa                                                                                      <  2:4569253/150‑1 (MQ=255)
                                                               gAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGgaagaa                                                                                      <  2:3372459/150‑1 (MQ=255)
                                                                aGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGaagaag                                                                                     <  1:3135849/150‑1 (MQ=255)
                                                                  ggCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCACGCTGAAGATCGGTAACGGCCTGGAAGAAGGc                                                                                   <  1:2467682/150‑1 (MQ=255)
                                                                  ggCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGc                                                                                   <  1:1641307/150‑1 (MQ=255)
                                                                  ggCGCGATCATATCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGc                                                                                   >  2:2023795/1‑150 (MQ=255)
                                                                   gcgcGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGcc                                                                                  >  2:3708538/1‑149 (MQ=255)
                                                                       gATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGcaccac                                                                              <  2:4752501/150‑1 (MQ=255)
                                                                       gATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGcaccac                                                                              <  2:4752472/150‑1 (MQ=255)
                                                                           atcTCCAAGTACCGTAACAACGGCCAGACCTGCGTCCGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACt                                                                          >  2:3618388/1‑150 (MQ=255)
                                                                            tctcCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTg                                                                         <  2:2726515/150‑1 (MQ=255)
                                                                            actcCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTg                                                                         <  1:2398132/149‑1 (MQ=255)
                                                                               ccAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGcc                                                                      <  2:123899/150‑1 (MQ=255)
                                                                               ccAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGcc                                                                      <  1:4025179/150‑1 (MQ=255)
                                                                               ccAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGcc                                                                      >  1:4112019/1‑150 (MQ=255)
                                                                               ccAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGcc                                                                      >  2:939710/1‑150 (MQ=255)
                                                                                cAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGccc                                                                     >  2:5053341/1‑150 (MQ=255)
                                                                                    tACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTg                                                                 >  2:854582/1‑150 (MQ=255)
                                                                                       cgcaacaacGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCTCTCCAAGGCGGTCGGCTCGCTCAAGACCGGCAACGGCTTCGACGAGGGCATCAATCTCGGCCCGCTGATc                                                              >  1:3565105/4‑150 (MQ=255)
                                                                                       cGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATc                                                              >  2:2349112/1‑150 (MQ=255)
                                                                                       cGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATc                                                              <  2:1138145/150‑1 (MQ=255)
                                                                                        gcaacaacGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCTCTCCAAGGCGGTCGGCTCGCTCAAGACCGGCAACGGCTTCGACGAGGGCATCAATCTCGGCCCGCTGATCg                                                             >  1:2793585/3‑150 (MQ=255)
                                                                                          aacaacGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGAt                                                           <  1:3202225/150‑1 (MQ=255)
                                                                                          aacaacGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGAt                                                           >  2:4151154/1‑150 (MQ=255)
                                                                                           acaacGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCTCTCCAAGGCGGTCGGCTCGCTCAAGACCGGCAACGGCTTCGACGAGGGCATCAATCTCGGCCCGCTGATCGATg                                                          <  1:1079911/150‑1 (MQ=255)
                                                                                            caccGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCTCTCCAAGGCGGTCGGCTCGCTCAAGACCGGCAACGGCTTCGACGAGGGCATCAATCTCGGCCCGCTGATCGATga                                                         <  2:4844797/147‑2 (MQ=255)
                                                                                             aacGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCTCTCCAAGGCGGTCGGCTCGCTCAAGACCGGCAACGGCTTCGACGAGGGCATCAATCTCGGCCCGCTGATCGATgag                                                        <  1:1545036/150‑3 (MQ=255)
                                                                                               cGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCaa                                                      <  2:4921342/150‑1 (MQ=255)
                                                                                                ggCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAg                                                     <  2:1242078/150‑1 (MQ=255)
                                                                                                  ccAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGc                                                   <  2:68800/150‑1 (MQ=255)
                                                                                                   cAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGTACGGCGTATACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGTAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCt                                                  <  1:337368/150‑1 (MQ=255)
                                                                                                   cAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCt                                                  >  1:1944774/1‑150 (MQ=255)
                                                                                                      aCCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTc                                               <  2:3557138/150‑1 (MQ=255)
                                                                                                           cGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCaa                                          <  2:1957408/150‑1 (MQ=255)
                                                                                                            gTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAg                                         <  1:897791/150‑1 (MQ=255)
                                                                                                                gcgcCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTcc                                     <  2:4008619/150‑1 (MQ=255)
                                                                                                                 cgcCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCa                                    <  2:1234806/150‑1 (MQ=255)
                                                                                                                 cgcCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCa                                    <  2:1631468/150‑1 (MQ=255)
                                                                                                                      aCCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAac                               <  1:3677006/150‑1 (MQ=255)
                                                                                                                       ccGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAaca                              >  1:4932311/1‑150 (MQ=255)
                                                                                                                             cTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGa                        <  1:2894941/150‑1 (MQ=255)
                                                                                                                              tACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAg                       <  1:4917792/150‑1 (MQ=255)
                                                                                                                               aCGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAgg                      <  1:3740733/150‑1 (MQ=255)
                                                                                                                                cGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGa                     <  1:4240025/150‑1 (MQ=255)
                                                                                                                                          ggCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGc           <  2:3740462/150‑1 (MQ=255)
                                                                                                                                              tCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAg       >  2:5117732/1‑150 (MQ=255)
                                                                                                                                               cTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAgg      <  1:4529660/150‑1 (MQ=255)
                                                                                                                                                  cgacgCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGgcgc   <  2:3553991/150‑1 (MQ=255)
                                                                                                                                                   gacgCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGcc  >  1:112801/1‑150 (MQ=255)
                                                                                                                                                     cgcgTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGAt                                                           <  1:4852241/91‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGCC  >  NC_002947/264009‑264305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑