BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,764,912	A→T	9.6%	I64F (ATC→TTC)	PP_3326 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,764,912	0	A	T	9.6%	521.7 / 29.9	171	I64F (ATC→TTC)	PP_3326	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (52/102); new base T (8/9); total (60/111)
Fisher's exact test for biased strand distribution p-value = 2.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 172 total aligned reads displayed.

TGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGA  >  NC_002947/3764763‑3765060

tGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGa                                                                                                                                                       <  2:2598892/150‑1 (MQ=255)

  gTCAGCGTCAACACAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATc                                                                                                                                                     <  2:1172488/150‑1 (MQ=255)

    cAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGATCATGTTccc                                                                                                                                                   <  1:4143720/150‑1 (MQ=255)

     aGCGTCAACTCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCg                                                                                                                                                  <  2:2054420/150‑1 (MQ=255)

     aGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGATCATGTTCCCg                                                                                                                                                  <  1:1437504/150‑1 (MQ=255)

        gTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGATCATGTTCCCGGTc                                                                                                                                               <  1:3359219/150‑1 (MQ=255)

        gTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGACCATGTTCCCGGTc                                                                                                                                               <  1:4380952/150‑1 (MQ=255)

         tCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCa                                                                                                                                              <  2:4016460/150‑1 (MQ=255)

           aaCGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACg                                                                                                                                            <  2:2903229/150‑1 (MQ=255)

           aaCGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACg                                                                                                                                            <  1:307166/150‑1 (MQ=255)

           aaCGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACg                                                                                                                                            <  1:2258367/150‑1 (MQ=255)

            aCGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGAtcccggtcccgg                                                                                                                                           >  1:3030046/1‑150 (MQ=255)

            aCGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACgg                                                                                                                                           >  2:1334333/1‑150 (MQ=255)

             cGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGt                                                                                                                                          <  1:776748/150‑1 (MQ=255)

              gCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTc                                                                                                                                         <  2:824728/150‑1 (MQ=255)

                aGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGa                                                                                                                                       >  1:4831143/1‑150 (MQ=255)

                aGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGa                                                                                                                                       >  1:4831485/1‑150 (MQ=255)

                 ggCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAg                                                                                                                                      <  1:643708/150‑1 (MQ=255)

                  gcgcCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAgc                                                                                                                                     <  2:1886996/150‑1 (MQ=255)

                  gcgcCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAgc                                                                                                                                     >  2:4681137/1‑150 (MQ=255)

                     cccACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGCGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑cc                                                                                                                                 <  2:2473780/150‑1 (MQ=255)

                      ccACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCt                                                                                                                                >  1:2367240/1‑150 (MQ=255)

                      ccACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCt                                                                                                                                >  1:1349685/1‑150 (MQ=255)

                           gCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCCCGGTCGAGCG‑CCTGGCgg                                                                                                                           >  1:86954/1‑150 (MQ=255)

                            ccTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGa                                                                                                                          <  2:486671/150‑1 (MQ=255)

                               gtgGACGGAGGAGGGAGGTTGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCa                                                                                                                       <  1:1034314/150‑1 (MQ=255)

                                tgGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCAt                                                                                                                      <  1:3671577/150‑1 (MQ=255)

                                tgGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCAt                                                                                                                      <  1:4724936/150‑1 (MQ=255)

                                     cGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGt                                                                                                                 >  1:1204093/1‑150 (MQ=255)

                                     cGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGt                                                                                                                 >  2:2370793/1‑150 (MQ=255)

                                        ggagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGtgct                                                                                                              <  1:4924517/150‑1 (MQ=255)

                                        ggagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGtgct                                                                                                              <  1:3850748/150‑1 (MQ=255)

                                         gagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGCACCAGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCAGGCCGtgctg                                                                                                             >  1:2566098/1‑150 (MQ=255)

                                         gagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGtgctg                                                                                                             >  1:2421437/1‑150 (MQ=255)

                                          agCGACGTGGCAATATCGCGGCCGTGTACGGCCACGGCGCCGCGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGtgctgc                                                                                                            <  1:2894109/150‑1 (MQ=255)

                                                gTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCAc                                                                                                      <  1:659531/150‑1 (MQ=255)

                                                     aaTATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTAcc                                                                                                 >  1:1669476/1‑150 (MQ=255)

                                                        gccgtggCCGTACACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTTACCGGGAACAAGATACCGGTCACGGTCGAGCG‑CCAGGCGGATCATGCCGTGCTGCAACCACTTAccccc                                                                                              >  1:2964355/6‑149 (MQ=255)

                                                        atCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACgg                                                                                                                                           >  2:1623007/1‑106 (MQ=255)

                                                              gCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCg                                                                                        <  2:2515871/150‑1 (MQ=255)

                                                              gCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCg                                                                                        <  1:3013080/150‑1 (MQ=255)

                                                                   gtACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACt                                                                                   >  2:1757364/1‑150 (MQ=255)

                                                                    tACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACtt                                                                                  <  2:4911503/150‑1 (MQ=255)

                                                                    tACGGCCACGGCGCCGAGGAGACCGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACtt                                                                                  <  2:307774/150‑1 (MQ=255)

                                                                          cACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGtg                                                                            <  2:719401/150‑1 (MQ=255)

                                                                           aCGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGttc                                                                           <  1:3145996/150‑3 (MQ=255)

                                                                           aCGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGtgc                                                                           <  2:2817570/150‑1 (MQ=255)

                                                                              gcgcCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGtgctgc                                                                        <  1:2709690/150‑1 (MQ=255)

                                                                              gcgcCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGtgctgc                                                                        <  1:568920/150‑1 (MQ=255)

                                                                              gcgcCGAGGAGACCGCCTACAAGGCGGAAAAGATCACGCGCGCCGGGGCGTTCGACGTGACCGGGAACAGGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGACGtgctgc                                                                        <  2:3359976/150‑1 (MQ=255)

                                                                                       aTAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACg                                                               <  2:3979613/150‑1 (MQ=255)

                                                                                       aTAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACg                                                               <  1:1488823/150‑1 (MQ=255)

                                                                                       aTAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACg                                                               <  2:4859267/150‑1 (MQ=255)

                                                                                        tAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACgg                                                              <  2:1118717/150‑1 (MQ=255)

                                                                                        tAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACgg                                                              >  2:1606271/1‑150 (MQ=255)

                                                                                             ccTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACCACGGGCCtt                                                         >  2:1363841/1‑150 (MQ=255)

                                                                                             ccTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCtt                                                         <  1:2310854/150‑1 (MQ=255)

                                                                                              cTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTg                                                        <  1:4344237/150‑1 (MQ=255)

                                                                                              cTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTg                                                        <  1:1594831/150‑1 (MQ=255)

                                                                                              cTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTAACCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCATg                                                        >  1:4432277/1‑150 (MQ=255)

                                                                                                   aGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTcac                                                   <  1:3417838/150‑1 (MQ=255)

                                                                                                    ggCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTcaca                                                  <  1:4318859/150‑1 (MQ=255)

                                                                                                    ggCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATACCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCCCCTGCGGCACGTGCCGTGCTGCTGGACAACGGGCCTTGGTcacc                                                  >  1:1334956/1‑149 (MQ=255)

                                                                                                        gAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAgcgc                                              <  2:679612/150‑1 (MQ=255)

                                                                                                         aaaaGATCAAGCGCGCCTGGGCGTTCGACGTGATCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGcc                                             <  2:826667/150‑1 (MQ=255)

                                                                                                          aaaGATCAAGCGCGCCGGGGCGTTCGACGTGACCGGGCACATGTTCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGccc                                            >  2:32274/1‑150 (MQ=255)

                                                                                                           aaGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCg                                           >  1:2098060/1‑150 (MQ=255)

                                                                                                              aTCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGAc                                        <  1:1757364/150‑1 (MQ=255)

                                                                                                              aTCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACAAGATCCCGGGCACGGTCGAGCG‑CCTGGCGGATCATGCCGGGCGGCAACCACTTACCCCCCCAGCGGCACTTGCCGTGCTGCTGGACAACGGGCCGTGGTCACAGCGCCCGGAc                                        >  1:1923137/1‑150 (MQ=255)

                                                                                                               tCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGa                                                                                                                                       >  2:381016/1‑55 (MQ=255)

                                                                                                               tCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGa                                                                                                                                       <  1:381016/55‑1 (MQ=255)

                                                                                                                 aaGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTACCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAg                                     <  2:1495305/150‑1 (MQ=255)

                                                                                                                      cgcCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATg                                <  2:3212744/150‑1 (MQ=255)

                                                                                                                       gcCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATgg                               <  2:4031778/150‑1 (MQ=255)

                                                                                                                       gcCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATgg                               <  1:2238917/150‑1 (MQ=255)

                                                                                                                       gcCTGGGCGTTCGACGTGACCCGGAACATGATACCCGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCCCCGGCGGCACTAGCCGTGCTGCTGGACAACGGGCCGTGGTCACAGCGCCCGGACAAGCAATgg                               >  1:917750/1‑150 (MQ=255)

                                                                                                                         cTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGt                             <  1:2930576/150‑1 (MQ=255)

                                                                                                                         cTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGt                             <  1:4923473/150‑1 (MQ=255)

                                                                                                                                ttCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAg                      <  1:263465/150‑1 (MQ=255)

                                                                                                                                ttCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAg                      <  1:1903300/150‑1 (MQ=255)

                                                                                                                                tcgaccGTGACCGGGCTCATGTTCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAg                      >  2:2243313/6‑150 (MQ=255)

                                                                                                                                tcgaccGTGACCGGGATCATGTTCCCGGTCAC‑GTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAgg                     >  2:4773294/6‑150 (MQ=39)

                                                                                                                                  cGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGa                    <  1:1293394/150‑1 (MQ=255)

                                                                                                                                  cGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGa                    <  1:2135765/150‑1 (MQ=255)

                                                                                                                                   gACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGAt                   >  2:4760154/1‑150 (MQ=255)

                                                                                                                                    aCGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATc                  <  2:4431766/150‑1 (MQ=255)

                                                                                                                                       tGACCGGGATCATGTTCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCACTGGGTCAATCAGGATCGCa               >  2:2258584/1‑150 (MQ=255)

                                                                                                                                       tGACCGGGATCATGTTCCCGGTCAC‑GTCGAACG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCAc              >  1:1649695/1‑150 (MQ=255)

                                                                                                                                       tGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCa               <  1:3883049/150‑1 (MQ=255)

                                                                                                                                         aCCGGGATCATGTTCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCAcc             >  1:4154452/1‑150 (MQ=255)

                                                                                                                                          ccGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTcaca                                                  >  1:1527763/1‑112 (MQ=255)

                                                                                                                                          ccGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTcaca                                                  <  2:1527763/112‑1 (MQ=255)

                                                                                                                                           cGGGATCATGTTCCCGGTCAC‑GTCGAACG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCAt          >  1:3724106/1‑150 (MQ=255)

                                                                                                                                            gggAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCAt          >  2:5006131/1‑150 (MQ=255)

                                                                                                                                              gatcATGTTCCCGGTCAC‑GTCGAACGCCCAGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGt        >  1:3442591/4‑150 (MQ=255)

                                                                                                                                                 cATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCaa     <  2:2421437/150‑1 (MQ=255)

                                                                                                                                                 cATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCaa     <  2:2566098/150‑1 (MQ=255)

                                                                                                                                                    gATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCaa     <  2:4500485/147‑1 (MQ=255)

                                                                                                                                                    gATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCaa     >  1:4500485/1‑147 (MQ=255)

                                                                                                                                                    gATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGa  <  1:4681137/150‑1 (MQ=255)

TGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCG‑CCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGA  >  NC_002947/3764763‑3765060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`