BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,803,263	A→C	10.8%	V873V (GTT→GTG)	PP_3361 ←	aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,803,263	0	A	C	10.8%	534.0 / 44.9	176	V873V (GTT→GTG)	PP_3361	aminotransferase
Reads supporting (aligned to +/- strand): ref base A (85/72); new base C (4/15); total (89/87)
Fisher's exact test for biased strand distribution p-value = 7.33e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 185 total aligned reads displayed.

CCTCGAGCAGGTAATGGCCGACACTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAG  >  NC_002947/3803115‑3803412

ccTCGAGCAGGTAATGGCCGACACTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCaa                                                                                                                                                      >  2:1748596/1‑150 (MQ=255)

ccTCGAGCAGGTAATGGCCGACACTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCaa                                                                                                                                                      >  2:1748551/1‑150 (MQ=255)

ccTCGAGCAGGTAATGGCCGACACTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCaa                                                                                                                                                      <  2:89422/150‑1 (MQ=255)

ccTCGAGCAGGTAATGGCCGACACTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCaa                                                                                                                                                      >  2:5087398/1‑150 (MQ=255)

ccTCGAGCAGGTAATGGCCGACACTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCaa                                                                                                                                                      <  1:3742058/150‑1 (MQ=255)

  tCGAGCAGGTAATGGCCGACACTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAAcc                                                                                                                                                    >  2:1882206/1‑150 (MQ=255)

      gCAGGTAATGGCCGACACTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCACGCCGTTGTTGAAAGC‑ATCGGCAACCTcgc                                                                                                                                                >  2:800229/1‑150 (MQ=255)

        aGGTAATGGCCGACACTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTcgcgc                                                                                                                                              >  2:3188332/1‑150 (MQ=255)

          gTAATGGCCGACACTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGt                                                                                                                                            >  1:5078056/1‑150 (MQ=255)

          gTAATGGCCGACACTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGAATTGAAATACTCCATGCCGTAGTTGAAAGC‑ATCGGCAACCTCGCGCGt                                                                                                                                            >  1:5078205/1‑150 (MQ=255)

           tAATGGCCGACACTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTg                                                                                                                                           <  2:524719/150‑1 (MQ=255)

           tAATGGCCGACACTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTg                                                                                                                                           <  1:1006918/150‑1 (MQ=255)

            aaTGGCCGACACTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTgg                                                                                                                                          <  2:4058533/150‑1 (MQ=255)

            aaTGGCCGACACTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTgg                                                                                                                                          >  1:1814305/1‑150 (MQ=255)

              tGGCCGACACTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTc                                                                                                                                        <  2:5022027/150‑1 (MQ=255)

                gCCGACACTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCAc                                                                                                                                      >  2:1248112/1‑150 (MQ=255)

                  cGACACTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTa                                                                                                                                    <  2:614665/150‑1 (MQ=255)

                       cTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTAccgcc                                                                                                                               <  1:1167891/150‑1 (MQ=255)

                         cAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGa                                                                                                                             <  2:3600810/150‑1 (MQ=255)

                          aaGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGAc                                                                                                                            >  1:303239/1‑150 (MQ=255)

                             gCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATc                                                                                                                         >  1:3398924/1‑150 (MQ=255)

                               gTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCgg                                                                                                                       >  1:790338/1‑150 (MQ=255)

                                ttttCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCggg                                                                                                                      <  1:4679164/150‑1 (MQ=255)

                                ttttCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCggg                                                                                                                      <  2:3856406/150‑1 (MQ=255)

                                  ttctttcAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTg                                                                                                                    <  1:3179023/150‑1 (MQ=255)

                                   tctttcAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTgg                                                                                                                   <  2:4781846/150‑1 (MQ=255)

                                       tcAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGt                                                                                                               >  2:1700108/1‑150 (MQ=255)

                                         aaCTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTa                                                                                                             >  2:1426261/1‑150 (MQ=255)

                                          aCTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTAc                                                                                                            <  1:1977325/150‑1 (MQ=255)

                                           cTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTAcc                                                                                                           >  1:2955152/1‑150 (MQ=255)

                                               tCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATg                                                                                                       >  1:1569631/1‑150 (MQ=255)

                                                 gcgcTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATggg                                                                                                     <  1:2876843/150‑1 (MQ=255)

                                                    cTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGttt                                                                                                  <  2:4694425/150‑1 (MQ=255)

                                                     tCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTg                                                                                                 >  1:2576147/1‑150 (MQ=255)

                                                     tCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTg                                                                                                 >  1:4782619/1‑150 (MQ=255)

                                                     tCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTg                                                                                                 >  1:2766207/1‑150 (MQ=255)

                                                      cgatcacATTGAGCACTGCGAGGCCTGCGGCACAGGAAACCGGATTGCCACCGAAGGTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGc                                                                                                <  1:3069212/144‑1 (MQ=255)

                                                      cgatcacATTGAGCACTGCGAGACCTGCGGCACAGGAAACCGGATTGCCACCGAAGGTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGc                                                                                                <  1:4022479/144‑1 (MQ=255)

                                                      cGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGc                                                                                                <  1:2701685/150‑1 (MQ=255)

                                                       gatcacATTGAGCACTGCGAGGCCTGCGGCACAGGAAACCGGATTGCCACCGAAGGTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGcc                                                                                               <  1:2633913/145‑1 (MQ=255)

                                                           gCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATg                                                                                           <  2:3159581/150‑1 (MQ=255)

                                                            cATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGc                                                                                          >  2:4185135/1‑150 (MQ=255)

                                                             aTCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCt                                                                                         >  2:4414818/1‑150 (MQ=255)

                                                              ttgAGCACTGCGAGGCCTGCGGCACAGGAAACCGGATTGCCACCGAAGGTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCAtagtg                                                                                        >  2:1060468/3‑146 (MQ=255)

                                                              tCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGtt                                                                                                              >  2:2402746/1‑128 (MQ=255)

                                                              tCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTg                                                                                        >  1:1176475/1‑150 (MQ=255)

                                                              tCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTg                                                                                        >  2:4420801/1‑150 (MQ=255)

                                                                   tACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGc                                                                                   >  2:1103992/1‑150 (MQ=255)

                                                                     ccGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCAt                                                                                 <  1:4405346/150‑1 (MQ=255)

                                                                        ccAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCg                                                                              >  1:1650618/1‑150 (MQ=255)

                                                                         cAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCgg                                                                             >  1:4580501/1‑150 (MQ=255)

                                                                         cAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCgg                                                                             <  1:2480007/150‑1 (MQ=255)

                                                                         cAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCgg                                                                             <  1:608532/150‑1 (MQ=255)

                                                                           ggCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGa                                                                           <  2:3415916/150‑1 (MQ=255)

                                                                              cTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCa                                                                        >  1:3234732/1‑150 (MQ=255)

                                                                                aCCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACg                                                                      >  1:2518073/1‑150 (MQ=255)

                                                                                     gcATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTg                                                                 >  1:2637788/1‑150 (MQ=255)

                                                                                      cATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTgg                                                                <  1:2677806/150‑1 (MQ=255)

                                                                                       aTGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTggg                                                               >  2:4663135/1‑150 (MQ=255)

                                                                                       aTGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTggg                                                               >  2:4587025/1‑150 (MQ=255)

                                                                                            acCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGTAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCTTCCGGGACCACGCCCTGGGTCTCg                                                          >  2:3836236/1‑150 (MQ=255)

                                                                                            acCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCg                                                          >  1:1043398/1‑150 (MQ=255)

                                                                                            acCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCg                                                          >  1:2212589/1‑150 (MQ=255)

                                                                                              cGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATg                                                                                                       <  2:3257519/103‑1 (MQ=255)

                                                                                                  ttgccaccGAAGGTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGcc                                                    <  1:2170854/143‑1 (MQ=255)

                                                                                                  ttACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGcc                                                    <  1:4694406/150‑1 (MQ=255)

                                                                                                  tagccaccGAAGGTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGcc                                                    <  1:3255659/143‑1 (MQ=255)

                                                                                                     ccGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCccac                                                 <  1:2823616/150‑1 (MQ=255)

                                                                                                     ccGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCccac                                                 <  2:3898604/150‑1 (MQ=255)

                                                                                                       ggCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCccacca                                               <  1:1512929/150‑1 (MQ=255)

                                                                                                       accGAAGGTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCccacca                                               >  2:796280/3‑150 (MQ=255)

                                                                                                       accGAAGGTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCccacca                                               <  1:2047916/148‑1 (MQ=255)

                                                                                                         cGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGt                                             <  2:162416/150‑1 (MQ=255)

                                                                                                           aaGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTgg                                           <  1:82950/150‑1 (MQ=255)

                                                                                                            aGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGc                                          >  1:1263474/1‑150 (MQ=255)

                                                                                                             ggTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCt                                         <  2:1732259/150‑1 (MQ=255)

                                                                                                             ggTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCt                                         <  1:1700108/150‑1 (MQ=255)

                                                                                                                   gAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGAc                                   <  1:3601631/150‑1 (MQ=255)

                                                                                                                    aaaTATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCTGCCTAcg                                  >  1:2994128/1‑149 (MQ=255)

                                                                                                                     aaTATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCTGCCTAcgc                                 <  1:5017415/150‑3 (MQ=255)

                                                                                                                       tACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCgg                               <  2:4840898/150‑1 (MQ=255)

                                                                                                                            cATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGaa                          <  1:3477964/150‑1 (MQ=255)

                                                                                                                            cATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGaa                          >  1:1920909/1‑150 (MQ=255)

                                                                                                                            cATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCTGCCTACGCGACCaaa                          <  1:378132/150‑1 (MQ=255)

                                                                                                                            cATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCTGCCTACGCGACCaaa                          <  1:1505301/150‑1 (MQ=255)

                                                                                                                             aTGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAAc                         <  2:3723453/150‑1 (MQ=255)

                                                                                                                             aTGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAAc                         <  1:4167095/150‑1 (MQ=255)

                                                                                                                             aTGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCAGCCGAAc                         <  2:1814305/150‑1 (MQ=255)

                                                                                                                                 cGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGa                     <  2:1263474/150‑1 (MQ=255)

                                                                                                                                 cGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGa                     >  2:3417207/1‑150 (MQ=255)

                                                                                                                                 cGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGGTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGa                     >  2:2935253/1‑150 (MQ=255)

                                                                                                                                  gttgttGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGAc                    >  1:4973080/1‑150 (MQ=255)

                                                                                                                                      ttgAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGa                >  1:1618627/1‑150 (MQ=255)

                                                                                                                                       tgAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGaa               <  1:3268792/150‑1 (MQ=255)

                                                                                                                                        gAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGatc              >  2:4133715/1‑148 (MQ=255)

                                                                                                                                             c‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCAt         >  1:3809434/1‑150 (MQ=255)

                                                                                                                                                tCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCg       <  1:2902676/150‑1 (MQ=255)

                                                                                                                                                    cAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCa   <  1:5087398/150‑1 (MQ=255)

                                                                                                                                                    cAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCa   <  2:2448392/150‑1 (MQ=255)

                                                                                                                                                     aaCCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAg  <  1:3926053/150‑1 (MQ=255)

CCTCGAGCAGGTAATGGCCGACACTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAG  >  NC_002947/3803115‑3803412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`