BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,803,287	C→A	11.2%	S865S (TCG→TCT)	PP_3361 ←	aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,803,287	0	C	A	11.2%	511.1 / 41.9	167	S865S (TCG→TCT)	PP_3361	aminotransferase
Reads supporting (aligned to +/- strand): ref base C (79/69); new base A (4/15); total (83/84)
Fisher's exact test for biased strand distribution p-value = 1.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

CTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCG  >  NC_002947/3803138‑3803433

cTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTAccgcc                                                                                                                                                    <  1:1167891/150‑1 (MQ=255)

 tCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTAccgccg                                                                                                                                                   >  1:119331/1‑150 (MQ=255)

  cAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGa                                                                                                                                                  <  1:790962/150‑1 (MQ=255)

  cAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGa                                                                                                                                                  <  2:3600810/150‑1 (MQ=255)

   aaGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGAc                                                                                                                                                 >  1:303239/1‑150 (MQ=255)

      gCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATc                                                                                                                                              >  1:3398924/1‑150 (MQ=255)

         ttttCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCggg                                                                                                                                           <  1:4679164/150‑1 (MQ=255)

         ttttCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCggg                                                                                                                                           <  2:3856406/150‑1 (MQ=255)

           ttctttcAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTg                                                                                                                                         >  2:425489/1‑150 (MQ=255)

            tctttcAACTGGTCGCGCTCGAGTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACCTCGGGTgg                                                                                                                                        <  2:4781851/150‑1 (MQ=255)

              tttcAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGcc                                                                                                                                      >  1:2207175/1‑150 (MQ=255)

                tcAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGt                                                                                                                                    >  2:1700108/1‑150 (MQ=255)

                  aaCTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTa                                                                                                                                  >  2:1426261/1‑150 (MQ=255)

                    cTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTAcc                                                                                                                                >  1:2955152/1‑150 (MQ=255)

                     tGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCAGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCa                                                                                                                               >  1:1763051/1‑150 (MQ=255)

                       gTCGCGCTCGAATGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAAt                                                                                                                             <  2:3032887/150‑1 (MQ=255)

                         cgcgCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGGGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATgg                                                                                                                           <  2:3278656/150‑1 (MQ=255)

                          gcgcTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATggg                                                                                                                          <  1:2876843/150‑1 (MQ=255)

                          gcgcTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATggg                                                                                                                          >  2:4579391/1‑150 (MQ=255)

                            gcTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGtt                                                                                                                        >  2:807732/1‑150 (MQ=255)

                             cTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGttt                                                                                                                       <  2:4694425/150‑1 (MQ=255)

                              tCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTg                                                                                                                      >  1:2576147/1‑150 (MQ=255)

                              tCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTg                                                                                                                      >  1:4782619/1‑150 (MQ=255)

                               cgatcacATTGAGCACTGCGAGGCCTGCGGCACAGGAAACCGGATTGCCACCGAAGGTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGc                                                                                                                     <  1:3069233/144‑1 (MQ=255)

                               cgatcacATTGAGCACTGCGAGACCTGCGGCACAGGAAACCGGATTGCCACCGAAGGTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGc                                                                                                                     <  1:4022479/144‑1 (MQ=255)

                               cGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGc                                                                                                                     <  1:2701685/150‑1 (MQ=255)

                               cGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGc                                                                                                                     <  1:2701765/150‑1 (MQ=255)

                               cGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGc                                                                                                                     <  1:3209115/150‑1 (MQ=255)

                                gatcacATTGAGCACTGCGAGGCCTGCGGCACAGGAAACCGGATTGCCACCGAAGGTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGcc                                                                                                                    <  1:2633913/145‑1 (MQ=255)

                                  tcacATTGAGCACTGCGAGGCCTGCGGCACAGGAAACCGGATTGCCACCGAAGGTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCa                                                                                                                  >  2:5017415/4‑150 (MQ=255)

                                     cATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGc                                                                                                               >  2:4185135/1‑150 (MQ=255)

                                      aTCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCt                                                                                                              >  2:4414818/1‑150 (MQ=255)

                                       ttgAGCACTGCGAGGCCTGCGGCACAGGAAACCGGATTGCCACCGAAGGTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCAtagtg                                                                                                             >  2:1060468/3‑146 (MQ=255)

                                       tCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGtt                                                                                                                                   >  2:2402746/1‑128 (MQ=255)

                                       tCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGtt                                                                                                                                   <  1:2402746/128‑1 (MQ=255)

                                       tCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTg                                                                                                             >  1:1176475/1‑150 (MQ=255)

                                            tACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGc                                                                                                        >  2:1103992/1‑150 (MQ=255)

                                              ccGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCAt                                                                                                      <  1:4405346/150‑1 (MQ=255)

                                               cGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATc                                                                                                     >  2:4110914/1‑150 (MQ=255)

                                                 ccAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCg                                                                                                   >  1:1650618/1‑150 (MQ=255)

                                                  cAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCgg                                                                                                  >  1:4580501/1‑150 (MQ=255)

                                                  cAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCgg                                                                                                  <  1:608532/150‑1 (MQ=255)

                                                       cTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCa                                                                                             >  1:3234732/1‑150 (MQ=255)

                                                         aCCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACg                                                                                           >  1:2518073/1‑150 (MQ=255)

                                                              gcATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTg                                                                                      >  1:2637788/1‑150 (MQ=255)

                                                               cATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCCTGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTgg                                                                                     <  1:2508737/150‑1 (MQ=255)

                                                               cATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTgg                                                                                     <  1:2677806/150‑1 (MQ=255)

                                                                aTGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTggg                                                                                    >  2:4663135/1‑150 (MQ=255)

                                                                aTGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTggg                                                                                    >  2:4587025/1‑150 (MQ=255)

                                                                     acCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGTAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCTTCCGGGACCACGCCCTGGGTCTCg                                                                               >  2:3836236/1‑150 (MQ=255)

                                                                     acCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCg                                                                               >  2:3234878/1‑150 (MQ=255)

                                                                     acCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCg                                                                               >  1:1043398/1‑150 (MQ=255)

                                                                       cGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATg                                                                                                                            >  1:3257519/1‑103 (MQ=255)

                                                                           ttACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGcc                                                                         <  1:4694406/150‑1 (MQ=255)

                                                                            tACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGccc                                                                        >  1:1457912/1‑150 (MQ=255)

                                                                              ccGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCccac                                                                      <  1:2823616/150‑1 (MQ=255)

                                                                              ccGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCccac                                                                      <  2:3898604/150‑1 (MQ=255)

                                                                                ggCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCccacca                                                                    <  1:1512929/150‑1 (MQ=255)

                                                                                accGAAGGTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCccacca                                                                    <  1:2047916/148‑1 (MQ=255)

                                                                                  cGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGt                                                                  <  2:162416/150‑1 (MQ=255)

                                                                                   gAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTg                                                                 >  2:1728454/1‑150 (MQ=255)

                                                                                    aaGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTgg                                                                <  1:82950/150‑1 (MQ=255)

                                                                                     aGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGc                                                               >  1:1263474/1‑150 (MQ=255)

                                                                                      ggTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCt                                                              <  1:1700108/150‑1 (MQ=255)

                                                                                         aTTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGcc                                                           >  1:717244/1‑150 (MQ=255)

                                                                                            gAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGAc                                                        <  1:3601631/150‑1 (MQ=255)

                                                                                             aaaTATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCTGCCTAcg                                                       <  2:61277/150‑2 (MQ=255)

                                                                                             aaaTATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCTGCCTAcg                                                       >  1:2994128/1‑149 (MQ=255)

                                                                                              aaTATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCTGCCTAcgc                                                      <  1:5017415/150‑3 (MQ=255)

                                                                                                tACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCgg                                                    <  2:4840898/150‑1 (MQ=255)

                                                                                                   tCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCg                                                 >  1:336368/1‑150 (MQ=255)

                                                                                                     cATGCCGTTGTTGAAATC‑ATCGGCAACCTCGCGGGTGTTCACTACCGCCTACATCTGGTGGGCTTTACCAATGTTTTTGCCCATGCTGTCGTCTTCCGGGTCCACTCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGat                                               <  1:1601852/150‑2 (MQ=255)

                                                                                                     cATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGaa                                               <  1:3477964/150‑1 (MQ=255)

                                                                                                     cATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGaa                                               >  1:1920909/1‑150 (MQ=255)

                                                                                                     cATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCTGCCTACGCGACCaaa                                               <  1:1505374/150‑1 (MQ=255)

                                                                                                     cATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCTGCCTACGCGACCaaa                                               <  1:378038/150‑1 (MQ=255)

                                                                                                     cATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCTGCCTACGCGACCaaa                                               <  1:378132/150‑1 (MQ=255)

                                                                                                      aTGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCAGCCGAAc                                              <  2:1814305/150‑1 (MQ=255)

                                                                                                      aTGCCGTTGTTGAAAGC‑ATCGGCAACATCGCTCGTGGTCACTACCGCCGAAATCGGGTGGCCGTTACCAATGGGTTTGCCAATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGACGATGCCGACCCGGCCGAAc                                              >  2:1863307/1‑150 (MQ=255)

                                                                                                          cGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGa                                          >  2:3417204/1‑150 (MQ=255)

                                                                                                          cGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGa                                          <  2:1263474/150‑1 (MQ=255)

                                                                                                          cGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGGTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGa                                          >  2:2935253/1‑150 (MQ=255)

                                                                                                           gttgttGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGAc                                         >  1:4973080/1‑150 (MQ=255)

                                                                                                                        aTCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATc                             >  2:345634/1‑150 (MQ=255)

                                                                                                                        aTCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATc                             <  1:2102513/150‑1 (MQ=255)

                                                                                                                         tCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCg                            >  2:3734697/1‑150 (MQ=255)

                                                                                                                         tCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCg                            <  1:2902676/150‑1 (MQ=255)

                                                                                                                           ggCAACCTCGCGCGTGGTCACTACCGCCGACATCTGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGc                          >  2:681618/1‑150 (MQ=255)

                                                                                                                           ggCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGc                          >  1:3044679/1‑150 (MQ=255)

                                                                                                                             cAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCa                        <  1:5087398/150‑1 (MQ=255)

                                                                                                                             cAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCa                        <  2:2448392/150‑1 (MQ=255)

                                                                                                                              aaCCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAg                       <  1:3926053/150‑1 (MQ=255)

                                                                                                                                  tCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGcac                   >  2:3470661/1‑150 (MQ=255)

                                                                                                                                   cgcgcgTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGcaca                  <  1:4110914/150‑1 (MQ=255)

                                                                                                                                       cgTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGccc              <  1:4774573/150‑1 (MQ=255)

                                                                                                                                             cACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTc        <  2:1763051/150‑1 (MQ=255)

                                                                                                                                               cTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCgg      <  2:3395973/150‑1 (MQ=255)

                                                                                                                                                tACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGc     >  1:2689989/1‑150 (MQ=255)

                                                                                                                                                tACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGc     >  1:4015837/1‑150 (MQ=255)

                                                                                                                                                tACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGc     >  2:870080/1‑150 (MQ=255)

CTCAAGGCGTTTTCTTTCAACTGGTCGCGCTCGATTGCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCG  >  NC_002947/3803138‑3803433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`