BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,803,323	G→A	7.2%	S853S (AGC→AGT) ‡	PP_3361 ←	aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,803,323	0	G	A	7.2%	543.4 / 22.2	179	S853S (AGC→AGT) ‡	PP_3361	aminotransferase
Reads supporting (aligned to +/- strand): ref base G (88/78); new base A (2/11); total (90/89)
Fisher's exact test for biased strand distribution p-value = 9.80e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 181 total aligned reads displayed.

GCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCGGGCAA  >  NC_002947/3803174‑3803472

 cATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGc                                                                                                                                                      >  2:4185135/1‑150 (MQ=255)

  aTCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCt                                                                                                                                                     >  2:4039497/1‑150 (MQ=255)

   tCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTg                                                                                                                                                    >  2:4420801/1‑150 (MQ=255)

   tCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTg                                                                                                                                                    >  1:1176466/1‑150 (MQ=255)

    cGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGa                                                                                                                                                   >  1:2944221/1‑150 (MQ=255)

        tACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGc                                                                                                                                               >  2:1103992/1‑150 (MQ=255)

           cGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATc                                                                                                                                            >  2:4110914/1‑150 (MQ=255)

             ccAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCg                                                                                                                                          >  1:1650618/1‑150 (MQ=255)

              cAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCgg                                                                                                                                         <  1:4773421/150‑1 (MQ=255)

                          gcATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTg                                                                                                                             <  1:952983/150‑1 (MQ=255)

                           cATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCCTGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTgg                                                                                                                            <  1:4088603/150‑1 (MQ=255)

                           cATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCCTGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTgg                                                                                                                            <  1:2508737/150‑1 (MQ=255)

                           cATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTgg                                                                                                                            <  1:4924214/150‑1 (MQ=255)

                           cATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTgg                                                                                                                            <  1:2677806/150‑1 (MQ=255)

                              gACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGtc                                                                                                                         >  1:3530070/1‑150 (MQ=255)

                                 acCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGTAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCTTCCGGGACCACGCCCTGGGTCTCg                                                                                                                      >  2:3836236/1‑150 (MQ=255)

                                 acCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCg                                                                                                                      >  2:3234878/1‑150 (MQ=255)

                                 acCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCg                                                                                                                      >  1:1043398/1‑150 (MQ=255)

                                   cGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGaa                                                                                                                    <  1:1882206/150‑1 (MQ=255)

                                       ttgccaccGAAGGTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGcc                                                                                                                <  1:2170854/143‑1 (MQ=255)

                                       ttACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGcc                                                                                                                <  1:4694406/150‑1 (MQ=255)

                                       tagccaccGAAGGTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGcc                                                                                                                <  1:3255659/143‑1 (MQ=255)

                                        tACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGccc                                                                                                               >  1:1457912/1‑150 (MQ=255)

                                        tACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGccc                                                                                                               <  1:2597865/150‑1 (MQ=255)

                                          ccGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCccac                                                                                                             <  1:2823616/150‑1 (MQ=255)

                                          ccGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCccac                                                                                                             <  2:3898604/150‑1 (MQ=255)

                                            ggCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCccacca                                                                                                           >  1:2575615/1‑150 (MQ=255)

                                            ggCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCccacca                                                                                                           <  1:1512929/150‑1 (MQ=255)

                                            accGAAGGTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCccacca                                                                                                           >  2:796280/3‑150 (MQ=255)

                                              cGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGt                                                                                                         <  2:162416/150‑1 (MQ=255)

                                               gAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTg                                                                                                        >  2:1728454/1‑150 (MQ=255)

                                               gAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTg                                                                                                        >  2:4592070/1‑150 (MQ=255)

                                                 aGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGc                                                                                                      >  1:1263474/1‑150 (MQ=255)

                                                  ggTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCt                                                                                                     <  2:1732259/150‑1 (MQ=255)

                                                     aTTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGcc                                                                                                  >  1:4455298/1‑150 (MQ=255)

                                                         aaaTATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCTGCCTAcg                                                                                              >  1:2994128/1‑149 (MQ=255)

                                                          aaTATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCTGCCTAcgc                                                                                             <  1:5017415/150‑3 (MQ=255)

                                                            tACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCgg                                                                                           <  2:4840898/150‑1 (MQ=255)

                                                               tCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCg                                                                                        >  1:336368/1‑150 (MQ=255)

                                                                 cATGCCGTTGTTGAAATC‑ATCGGCAACCTCGCGGGTGTTCACTACCGCCTACATCTGGTGGGCTTTACCAATGTTTTTGCCCATGCTGTCGTCTTCCGGGTCCACTCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGat                                                                                      <  1:1601852/150‑2 (MQ=255)

                                                                 cATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGaa                                                                                      >  1:1920909/1‑150 (MQ=255)

                                                                 cATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCTGCCTACGCGACCaaa                                                                                      <  1:378132/150‑1 (MQ=255)

                                                                 cATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCTGCCTACGCGACCaaa                                                                                      <  1:1505374/150‑1 (MQ=255)

                                                                  aTGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAAc                                                                                     <  1:4167095/150‑1 (MQ=255)

                                                                  aTGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCAGCCGAAc                                                                                     <  2:1814305/150‑1 (MQ=255)

                                                                    gCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAAccc                                                                                   <  1:1692125/150‑1 (MQ=255)

                                                                      cGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGa                                                                                 >  2:3417204/1‑150 (MQ=255)

                                                                      cGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGa                                                                                 <  2:1263474/150‑1 (MQ=255)

                                                                       gttgttGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGAc                                                                                >  1:3469724/1‑150 (MQ=255)

                                                                                    aTCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATc                                                                    <  1:2102513/150‑1 (MQ=255)

                                                                                     tCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCg                                                                   >  2:3734697/1‑150 (MQ=255)

                                                                                       ggCAACCTCGCGCGTGGTCACTACCGCCGACATCTGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGc                                                                 >  2:681618/1‑150 (MQ=255)

                                                                                       ggCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGc                                                                 >  1:3044679/1‑150 (MQ=255)

                                                                                         cAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCa                                                               <  1:5087398/150‑1 (MQ=255)

                                                                                         cAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCa                                                               <  2:2448392/150‑1 (MQ=255)

                                                                                          aaCCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAg                                                              <  1:3926053/150‑1 (MQ=255)

                                                                                              tCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGcac                                                          >  2:3470645/1‑150 (MQ=255)

                                                                                              tCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGcac                                                          >  2:3470661/1‑150 (MQ=255)

                                                                                               cgcgcgTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCTGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGcaca                                                         <  2:2199200/150‑1 (MQ=255)

                                                                                                   cgTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGccc                                                     <  1:1190677/150‑1 (MQ=255)

                                                                                                       gTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCt                                                 >  2:4345141/1‑150 (MQ=255)

                                                                                                            tACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGc                                            >  2:870080/1‑150 (MQ=255)

                                                                                                            tACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGc                                            >  1:2689989/1‑150 (MQ=255)

                                                                                                            tACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGc                                            >  1:631518/1‑150 (MQ=255)

                                                                                                               cgccgACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCg                                         >  1:2850176/1‑150 (MQ=255)

                                                                                                                      aTCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATg                                  <  2:303239/150‑1 (MQ=255)

                                                                                                                       tCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATgg                                 >  1:1753812/1‑150 (MQ=255)

                                                                                                                         gggTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCg                               <  1:1426261/150‑1 (MQ=255)

                                                                                                                         gggTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCg                               >  1:3742253/1‑150 (MQ=255)

                                                                                                                         gggTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCg                               >  2:2683971/1‑150 (MQ=255)

                                                                                                                          ggTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGt                              >  1:3529355/1‑150 (MQ=255)

                                                                                                                            tGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTac                            <  2:2576147/150‑1 (MQ=255)

                                                                                                                              gCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTacac                          <  1:1728454/150‑1 (MQ=255)

                                                                                                                              gCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTacac                          >  2:4576257/1‑150 (MQ=255)

                                                                                                                                cGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACAcct                        >  1:604768/1‑150 (MQ=255)

                                                                                                                                cGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACAcct                        >  1:604736/1‑150 (MQ=255)

                                                                                                                                    aCCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCtt                    <  1:800229/150‑1 (MQ=255)

                                                                                                                                     ccAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTg                   >  1:828802/1‑150 (MQ=255)

                                                                                                                                      cAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGa                  <  1:3188332/150‑1 (MQ=255)

                                                                                                                                      cAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGa                  >  2:3705474/1‑150 (MQ=255)

                                                                                                                                      cAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGa                  >  1:3112535/1‑150 (MQ=255)

                                                                                                                                      cAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGa                  >  2:1052057/1‑150 (MQ=255)

                                                                                                                                       aaTGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTTGGCTCGCACCATGGCGTACACCTCCTTGAg                 >  2:2878929/1‑150 (MQ=255)

                                                                                                                                       aaTGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCTTGGCGTACACCTCCTTGAg                 <  2:5078205/150‑1 (MQ=255)

                                                                                                                                       aaTGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAg                 <  2:5078093/150‑1 (MQ=255)

                                                                                                                                       aaTGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAg                 <  2:3398924/150‑1 (MQ=255)

                                                                                                                                       aaTGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAg                 <  1:1748551/150‑1 (MQ=255)

                                                                                                                                        aTGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCccacca                                                                                                           >  1:4260704/1‑59 (MQ=255)

                                                                                                                                        aTGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAgg                <  2:4580501/150‑1 (MQ=255)

                                                                                                                                         tGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGt               <  1:4579391/150‑1 (MQ=255)

                                                                                                                                          gggTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTa              >  1:2641545/1‑150 (MQ=255)

                                                                                                                                          gggTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTa              >  2:51024/1‑150 (MQ=255)

                                                                                                                                                 cccATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCg       >  1:1082863/1‑150 (MQ=255)

                                                                                                                                                 cccATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCg       <  1:960927/150‑1 (MQ=255)

                                                                                                                                                  ccATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCgg      >  2:5031008/1‑150 (MQ=255)

                                                                                                                                                  ccATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCgg      >  1:2277805/1‑150 (MQ=255)

                                                                                                                                                  ccATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCgg      <  2:3234732/150‑1 (MQ=255)

                                                                                                                                                  ccATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCgg      <  2:2212589/150‑1 (MQ=255)

                                                                                                                                                    aTGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCGGGc    >  1:462161/1‑150 (MQ=255)

                                                                                                                                                      gCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCGGGCaa  >  2:3437363/1‑150 (MQ=255)

GCATCGAGTACCGCCAGGCCTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCGGGCAA  >  NC_002947/3803174‑3803472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`