Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 264,180 G→A 5.3% A311T (GCT→ACT) ‡ gabD‑I → succinate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947264,1800GA 5.3% 470.8 / 10.1 149A311T (GCT→ACT) ‡gabD‑Isuccinate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (55/86);  new base A (5/3);  total (60/89)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 150 total aligned reads displayed.

ATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGCCAAAGTGCTGTCCGGTGGCAAGCTG  >  NC_002947/264036‑264329
                                                                                                                                                |                                                                                                                                                     
aTCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCa                                                                                                                                                  >  1:1294339/1‑150 (MQ=255)
  cGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGt                                                                                                                                                >  1:4239681/1‑150 (MQ=255)
  cGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGt                                                                                                                                                <  1:2412087/150‑1 (MQ=255)
       tCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCa                                                                                                                                           >  2:4529660/1‑150 (MQ=255)
         gacgacGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAg                                                                                                                                         >  1:3699086/1‑150 (MQ=255)
          acgacgCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGc                                                                                                                                        <  1:3690630/150‑1 (MQ=255)
          acgacgCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGc                                                                                                                                        <  1:2029679/150‑1 (MQ=255)
          acgacgCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGc                                                                                                                                        <  1:2029958/150‑1 (MQ=255)
            gacgCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTg                                                                                                                                      >  1:4296898/1‑150 (MQ=255)
               gCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAg                                                                                                                                   >  1:2453374/1‑150 (MQ=255)
                 cGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGAt                                                                                                                                 <  1:749825/150‑1 (MQ=255)
                 cGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGAt                                                                                                                                 <  1:2519124/150‑1 (MQ=255)
                  gACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATc                                                                                                                                >  1:1627380/1‑150 (MQ=255)
                  gACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATc                                                                                                                                >  1:1627621/1‑150 (MQ=255)
                   aCCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCg                                                                                                                               <  1:2508257/150‑1 (MQ=255)
                   aCCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCg                                                                                                                               <  1:1033305/150‑1 (MQ=255)
                   aCCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCg                                                                                                                               <  2:1998208/150‑1 (MQ=255)
                   aCCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCg                                                                                                                               <  1:4457006/150‑1 (MQ=255)
                   aCCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCg                                                                                                                               <  1:5079819/150‑1 (MQ=255)
                     cTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGt                                                                                                                             >  2:56306/1‑150 (MQ=255)
                     cTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGt                                                                                                                             >  2:3573236/1‑150 (MQ=255)
                        gACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAAc                                                                                                                          >  1:3948916/1‑150 (MQ=255)
                        gACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAAc                                                                                                                          >  1:4373271/1‑150 (MQ=255)
                          cAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGACAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACgg                                                                                                                        >  2:2825377/1‑150 (MQ=255)
                           aaGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACggt                                                                                                                       >  1:3511453/1‑149 (MQ=255)
                           aaGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGc                                                                                                                       <  2:867351/150‑1 (MQ=255)
                             ggCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCt                                                                                                                     >  2:1111484/1‑150 (MQ=255)
                             ggCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCt                                                                                                                     >  1:833095/1‑150 (MQ=255)
                              gCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACAGTATCTACGAGCAGGACGGAGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTg                                                                                                                    >  2:1823443/1‑150 (MQ=255)
                                  tCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGgaag                                                                                                                >  2:1489979/1‑150 (MQ=255)
                                   cGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGgaaga                                                                                                               <  1:2896935/150‑1 (MQ=255)
                                    gAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGgaagaa                                                                                                              <  2:3372459/150‑1 (MQ=255)
                                    gAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGgaagaa                                                                                                              <  2:4569253/150‑1 (MQ=255)
                                     aGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGaagaag                                                                                                             <  1:3135849/150‑1 (MQ=255)
                                      gggCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAgg                                                                                                            >  2:2422001/1‑150 (MQ=255)
                                       ggCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCACGCTGAAGATCGGTAACGGCCTGGAAGAAGGc                                                                                                           <  1:2467682/150‑1 (MQ=255)
                                       ggCGCGATCATATCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGc                                                                                                           >  2:2023795/1‑150 (MQ=255)
                                        gcgcGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGcc                                                                                                          >  2:3708538/1‑149 (MQ=255)
                                            gATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGcaccac                                                                                                      <  2:4752501/150‑1 (MQ=255)
                                            gATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGcaccac                                                                                                      >  1:4630004/1‑150 (MQ=255)
                                            gATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGcaccac                                                                                                      <  2:4752472/150‑1 (MQ=255)
                                                atcTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACt                                                                                                  <  2:3976239/150‑1 (MQ=255)
                                                atcTCCAAGTACCGTAACAACGGCCAGACCTGCGTCCGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACt                                                                                                  >  2:3618388/1‑150 (MQ=255)
                                                 tctcCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTg                                                                                                 <  2:2726515/150‑1 (MQ=255)
                                                 actcCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTg                                                                                                 <  1:2398132/149‑1 (MQ=255)
                                                    ccAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGcc                                                                                              <  2:123899/150‑1 (MQ=255)
                                                    ccAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGcc                                                                                              >  2:939710/1‑150 (MQ=255)
                                                    ccAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGcc                                                                                              >  1:4112019/1‑150 (MQ=255)
                                                    ccAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGcc                                                                                              <  1:4025036/150‑1 (MQ=255)
                                                     cAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGccc                                                                                             >  2:5053341/1‑150 (MQ=255)
                                                     cAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGccc                                                                                             >  2:1231353/1‑150 (MQ=255)
                                                         tACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTg                                                                                         >  2:854582/1‑150 (MQ=255)
                                                           ccGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCTCTCCAAGGCGGTCGGCTCGCTCAAGACCGGCAACGGCTTCGACGAGGGCATCAATCTCGGCCCGCTGAt                                                                                       >  2:1735544/1‑150 (MQ=255)
                                                             gcaacaacGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCTCTCCAAGGCGGTCGGCTCGCTCAAGACCGGCAACGGCTTCGACGAGGGCATCAATCTCGGCCCGCTGATCg                                                                                     >  1:2793585/3‑150 (MQ=255)
                                                               aacaacGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGAt                                                                                   <  1:3202225/150‑1 (MQ=255)
                                                                acaacGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCTCTCCAAGGCGGTCGGCTCGCTCAAGACCGGCAACGGCTTCGACGAGGGCATCAATCTCGGCCCGCTGATCGATg                                                                                  <  1:1079911/150‑1 (MQ=255)
                                                                 caccGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCTCTCCAAGGCGGTCGGCTCGCTCAAGACCGGCAACGGCTTCGACGAGGGCATCAATCTCGGCCCGCTGATCGATga                                                                                 <  2:4844797/147‑2 (MQ=255)
                                                                 caacGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATgg                                                                                 >  2:1853090/1‑150 (MQ=255)
                                                                  aacGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCTCTCCAAGGCGGTCGGCTCGCTCAAGACCGGCAACGGCTTCGACGAGGGCATCAATCTCGGCCCGCTGATCGATgag                                                                                <  1:1545036/150‑3 (MQ=255)
                                                                   acGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCTCTCCAAGGCGGTCGGCTCGCTCAAGACCGGCAACGGCTTCGACGAGGGCATCAATCTCGGCCCGCTGATCGATgagg                                                                               >  2:2335111/1‑147 (MQ=255)
                                                                     ggCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAg                                                                             <  2:1242078/150‑1 (MQ=255)
                                                                     ggCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAg                                                                             <  1:3508096/150‑1 (MQ=255)
                                                                       ccAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGc                                                                           >  1:1355983/1‑150 (MQ=255)
                                                                       ccAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGc                                                                           <  2:68800/150‑1 (MQ=255)
                                                                       ccAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCTCTCCAAGGCGGTCGGCTCGCTCAAGACCGGCAACGGCTTCGACGAGGGCATCAATCTCGGCCCGCTGATCGATgaggcggc                                                                           >  1:2053030/1‑143 (MQ=255)
                                                                        cAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGTACGGCGTATACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGTAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCt                                                                          <  1:337368/150‑1 (MQ=255)
                                                                        cAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCt                                                                          <  2:919628/150‑1 (MQ=255)
                                                                           aCCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTc                                                                       <  2:3557086/150‑1 (MQ=255)
                                                                                    tGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTc                                                              >  2:5006577/1‑150 (MQ=255)
                                                                                    tGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTc                                                              <  1:1866792/150‑1 (MQ=255)
                                                                                     gcgcCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTcc                                                             <  2:4008619/150‑1 (MQ=255)
                                                                                      cgcCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCa                                                            <  2:1631468/150‑1 (MQ=255)
                                                                                      cgcCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCa                                                            <  2:1487734/150‑1 (MQ=255)
                                                                                      cgcCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCa                                                            <  2:1234806/150‑1 (MQ=255)
                                                                                        ccAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAgg                                                          >  2:4933152/1‑150 (MQ=255)
                                                                                           aCCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAac                                                       <  1:3677006/150‑1 (MQ=255)
                                                                                            ccGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAaca                                                      >  1:4932311/1‑150 (MQ=255)
                                                                                                 tCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCg                                                 >  2:4164722/1‑150 (MQ=255)
                                                                                                  cTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGa                                                <  1:2894941/150‑1 (MQ=255)
                                                                                                  cTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGa                                                <  1:4102834/150‑1 (MQ=255)
                                                                                                     cGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGa                                             <  1:4239922/150‑1 (MQ=255)
                                                                                                      gTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGAc                                            >  1:5037878/1‑150 (MQ=255)
                                                                                                               ggCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGc                                   <  2:3740462/150‑1 (MQ=255)
                                                                                                                     tACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGgc                             <  1:3566310/150‑1 (MQ=255)
                                                                                                                     tACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGgc                             >  1:4361887/1‑150 (MQ=255)
                                                                                                                       cgacgCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGgcgc                           <  2:3553991/150‑1 (MQ=255)
                                                                                                                        gacgCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGcc                          >  1:112801/1‑150 (MQ=255)
                                                                                                                          cgcgTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGAt                                                                                   >  2:4852241/1‑91 (MQ=255)
                                                                                                                          cgcgTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGAt                                                                                   <  1:4852241/91‑1 (MQ=255)
                                                                                                                           gcgTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGCCaaa                       <  1:1853090/150‑1 (MQ=255)
                                                                                                                           gcgTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGCCaaa                       <  2:4113681/150‑1 (MQ=255)
                                                                                                                              ttCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGCCAAAGTg                    <  1:3140683/150‑1 (MQ=255)
                                                                                                                              ttCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCACCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGCCAAAGTg                    <  2:2275999/150‑1 (MQ=255)
                                                                                                                                 gCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGCCAAAGTGCTg                 >  2:2552215/1‑150 (MQ=255)
                                                                                                                                  ccGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGCCAAAGTGCTGt                <  1:4919324/150‑1 (MQ=255)
                                                                                                                                   cGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGCCAAAGTGCTGTc               >  2:2423794/1‑150 (MQ=255)
                                                                                                                                       aaGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGCCAAAGTGCTGTCCGGt           <  1:1263880/150‑1 (MQ=255)
                                                                                                                                            ggCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGCCAAAGTGCTGTCCGGTGGCaa      <  2:4943141/150‑1 (MQ=255)
                                                                                                                                               cGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGCCAAAGTGCTGTCCGGTGGCAAGCt   <  1:960376/150‑1 (MQ=255)
                                                                                                                                               cGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGCCAAAGTGCTGTCCGGTGACAAGCt   >  1:1279659/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
ATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGCCAAAGTGCTGTCCGGTGGCAAGCTG  >  NC_002947/264036‑264329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑