BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,803,341	C→A	7.5%	V847V (GTG→GTT)	PP_3361 ←	aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,803,341	0	C	A	7.5%	593.6 / 26.4	181	V847V (GTG→GTT)	PP_3361	aminotransferase
Reads supporting (aligned to +/- strand): ref base C (89/78); new base A (3/11); total (92/89)
Fisher's exact test for biased strand distribution p-value = 2.65e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 182 total aligned reads displayed.

CTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCGGGCAAAAACACCTGCCCCGCCAC  >  NC_002947/3803193‑3803490

       gcATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTg                                                                                                                                               >  1:2637788/1‑150 (MQ=255)

        cATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCCTGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTgg                                                                                                                                              <  1:4088603/150‑1 (MQ=255)

        cATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCCTGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTgg                                                                                                                                              <  1:2508737/150‑1 (MQ=255)

        cATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTgg                                                                                                                                              <  1:2677806/150‑1 (MQ=255)

        cATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTgg                                                                                                                                              <  1:2677763/150‑1 (MQ=255)

         aTGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTggg                                                                                                                                             >  2:4587025/1‑150 (MQ=255)

         aTGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTggg                                                                                                                                             >  2:4663135/1‑150 (MQ=255)

              acCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCg                                                                                                                                        >  1:1043398/1‑150 (MQ=255)

              acCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCg                                                                                                                                        >  2:3234878/1‑150 (MQ=255)

              acCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCg                                                                                                                                        >  1:2212589/1‑150 (MQ=255)

                cGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGaa                                                                                                                                      <  2:3857074/150‑1 (MQ=255)

                    ttgccaccGAAGGTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGcc                                                                                                                                  <  1:3021213/143‑1 (MQ=255)

                    tagccaccGAAGGTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGcc                                                                                                                                  <  1:3255659/143‑1 (MQ=255)

                     tACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGccc                                                                                                                                 >  1:1457912/1‑150 (MQ=255)

                       ccGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCccac                                                                                                                               <  2:3898604/150‑1 (MQ=255)

                       ccGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCccac                                                                                                                               <  1:2823616/150‑1 (MQ=255)

                         ggCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCccacca                                                                                                                             >  2:2445079/1‑150 (MQ=255)

                         ggCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCccacca                                                                                                                             >  1:2575615/1‑150 (MQ=255)

                             aaGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTgg                                                                                                                         <  1:82950/150‑1 (MQ=255)

                               ggTGTTGAAATATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCt                                                                                                                       <  2:1732259/150‑1 (MQ=255)

                                  aTTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGcc                                                                                                                    >  1:4455298/1‑150 (MQ=255)

                                     gAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGAc                                                                                                                 <  1:3601631/150‑1 (MQ=255)

                                      aaaTATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCTGCCTAcg                                                                                                                <  2:61277/150‑2 (MQ=255)

                                       aaTATTCCATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCTGCCTAcgc                                                                                                               <  1:5017415/150‑3 (MQ=255)

                                            tCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCg                                                                                                          >  1:336368/1‑150 (MQ=255)

                                              cATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGaa                                                                                                        >  1:1920909/1‑150 (MQ=255)

                                              cATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCTGCCTACGCGACCaaa                                                                                                        <  1:378038/150‑1 (MQ=255)

                                              cATGCCATTGTT‑AAAGCTGTCTGCCACTTCCCGCGTTGTGACAACCGCAGACATTGGATGACCGTTACCAATGGGTTTGCCCATAGTGACGATGTCAGGGACAACATCCTGCAACTCGAAAGCCCACCAGTGGCTGCCTACGCGACCaaa                                                                                                        <  1:1505301/150‑1 (MQ=255)

                                               aTGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAAc                                                                                                       <  1:4167095/150‑1 (MQ=255)

                                               aTGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAAc                                                                                                       <  2:3723453/150‑1 (MQ=255)

                                               aTGCCGTTGTTGAAAGC‑ATCGGCAACATCGCTCGTGGTCACTACCGCCGAAATCGGGTGGCCGTTACCAATGGGTTTGCCAATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGACGATGCCGACCCGGCCGAAc                                                                                                       >  2:1863307/1‑150 (MQ=255)

                                                 gCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAAccc                                                                                                     <  1:1692125/150‑1 (MQ=255)

                                                   cGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGa                                                                                                   >  2:3417207/1‑150 (MQ=255)

                                                   cGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGa                                                                                                   <  2:1263474/150‑1 (MQ=255)

                                                    gttgttGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGAc                                                                                                  >  1:3469724/1‑150 (MQ=255)

                                                    gttgttGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGAc                                                                                                  >  1:4973080/1‑150 (MQ=255)

                                                     ttgttgAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGAcc                                                                                                 >  2:1632493/1‑150 (MQ=255)

                                                        ttgAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGa                                                                                              >  1:1618627/1‑150 (MQ=255)

                                                          gAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAAc                                                                                            <  1:4684134/150‑1 (MQ=255)

                                                               c‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCAt                                                                                       >  1:3809434/1‑150 (MQ=255)

                                                                 aTCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATc                                                                                      <  1:2102513/150‑1 (MQ=255)

                                                                  tCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCg                                                                                     <  1:2902676/150‑1 (MQ=255)

                                                                    ggCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGc                                                                                   >  1:3044679/1‑150 (MQ=255)

                                                                      cAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCa                                                                                 <  1:5087398/150‑1 (MQ=255)

                                                                       aaCCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAg                                                                                <  1:3926053/150‑1 (MQ=255)

                                                                           tCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGcac                                                                            >  2:3470645/1‑150 (MQ=255)

                                                                            cgcgcgTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCTGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGcaca                                                                           <  2:2199200/150‑1 (MQ=255)

                                                                            cgcgcgTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGcaca                                                                           <  2:5007019/150‑1 (MQ=255)

                                                                               gcgTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGcc                                                                        <  2:1569631/150‑1 (MQ=255)

                                                                                cgTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGccc                                                                       <  1:4774573/150‑1 (MQ=255)

                                                                                cgTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGccc                                                                       <  1:1190677/150‑1 (MQ=255)

                                                                                     tCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCtt                                                                  >  2:842192/1‑150 (MQ=255)

                                                                                      cACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTc                                                                 >  2:3830490/1‑150 (MQ=255)

                                                                                      cACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTc                                                                 <  1:1274266/150‑1 (MQ=255)

                                                                                         tACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGc                                                              >  1:2689989/1‑150 (MQ=255)

                                                                                         tACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGc                                                              >  1:631518/1‑150 (MQ=255)

                                                                                                   aTCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATg                                                    <  2:303239/150‑1 (MQ=255)

                                                                                                      gggTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCg                                                 >  2:2683971/1‑150 (MQ=255)

                                                                                                       ggTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGt                                                <  1:807732/150‑1 (MQ=255)

                                                                                                       ggTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGt                                                >  1:3529355/1‑150 (MQ=255)

                                                                                                         tGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTac                                              <  2:2576147/150‑1 (MQ=255)

                                                                                                          ggCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTaca                                             <  1:1248112/150‑1 (MQ=255)

                                                                                                           gCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTacac                                            <  1:1728454/150‑1 (MQ=255)

                                                                                                             cGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACAcct                                          >  1:604736/1‑150 (MQ=255)

                                                                                                                 aCCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCtt                                      <  1:800229/150‑1 (MQ=255)

                                                                                                                   cAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGa                                    >  2:1052057/1‑150 (MQ=255)

                                                                                                                   cAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGa                                    <  1:3188332/150‑1 (MQ=255)

                                                                                                                   cAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGa                                    >  2:3667576/1‑150 (MQ=255)

                                                                                                                    aaTGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTTGGCTCGCACCATGGCGTACACCTCCTTGAg                                   >  2:2878929/1‑150 (MQ=255)

                                                                                                                    aaTGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCTTGGCGTACACCTCCTTGAg                                   <  2:5078205/150‑1 (MQ=255)

                                                                                                                    aaTGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAg                                   <  2:3398924/150‑1 (MQ=255)

                                                                                                                    aaTGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAg                                   <  1:1748504/150‑1 (MQ=255)

                                                                                                                    aaTGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAg                                   <  2:2784337/150‑1 (MQ=255)

                                                                                                                    aaTGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAg                                   <  1:4576257/150‑1 (MQ=255)

                                                                                                                    aaTGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAg                                   <  2:5078056/150‑1 (MQ=255)

                                                                                                                    aaTGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAg                                   <  2:790338/150‑1 (MQ=255)

                                                                                                                    aaTGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAg                                   <  1:1748596/150‑1 (MQ=255)

                                                                                                                     aTGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCccacca                                                                                                                             <  2:4260704/59‑1 (MQ=255)

                                                                                                                     aTGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAgg                                  <  2:4580501/150‑1 (MQ=255)

                                                                                                                     aTGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAgg                                  >  1:2139912/1‑150 (MQ=255)

                                                                                                                       gggTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTa                                >  2:50998/1‑150 (MQ=255)

                                                                                                                       gggTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTa                                <  1:1779560/150‑1 (MQ=255)

                                                                                                                              cccATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCg                         >  2:4505312/1‑150 (MQ=255)

                                                                                                                              cccATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCg                         >  1:1082863/1‑150 (MQ=255)

                                                                                                                              cccATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCg                         <  1:960927/150‑1 (MQ=255)

                                                                                                                               ccATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCgg                        <  2:2212589/150‑1 (MQ=255)

                                                                                                                                cATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCggg                       >  1:1585169/1‑150 (MQ=255)

                                                                                                                                cATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCggg                       >  1:1507955/1‑150 (MQ=255)

                                                                                                                                   gCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCGGGCaa                    >  2:3437363/1‑150 (MQ=255)

                                                                                                                                     tGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCGGGCaaaa                  <  2:1176475/150‑1 (MQ=255)

                                                                                                                                       aCGGCAACCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCGGGCAAAAac                >  1:3095269/1‑150 (MQ=255)

                                                                                                                                         ggCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCGGGCAAAAacac              >  1:3438380/1‑150 (MQ=255)

                                                                                                                                          gCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCGGGCAAAAACAcc             <  1:4420801/150‑1 (MQ=255)

                                                                                                                                           cATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCGGGCAAAAACACCt            <  1:4587025/150‑1 (MQ=255)

                                                                                                                                            aTCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCGGGCAAAAACACCTg           >  1:4223538/1‑150 (MQ=255)

                                                                                                                                            aTCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCGGGCAAAAACACCTg           >  2:905156/1‑150 (MQ=255)

                                                                                                                                            aTCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCGGACAAAAACACCTg           >  2:4025418/1‑150 (MQ=255)

                                                                                                                                             tCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCGGGCAAAAACACCTGc          >  2:473529/1‑150 (MQ=255)

                                                                                                                                               cGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCGGGCAAAAACACCTGccc        <  1:425489/150‑1 (MQ=255)

                                                                                                                                               cGGGACAACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGAACACCTCCTTGAGGTAATGTTCGGGCAAAAACACCTGccc        >  2:1776081/1‑150 (MQ=255)

CTACCGCGCATGACACCGGGTTACCGGCGAAGGTATTGAAATACTCCATGCCGTTGTTGAAAGC‑ATCGGCAACCTCGCGCGTGGTCACTACCGCCGACATCGGGTGGCCGTTACCAATGGGTTTGCCCATGCTGACGGCATCCGGGACCACGCCCTGGGTCTCGAATGCCCACCAGTGGCTGCCGACCCGGCCGAACCCGACCTGAACCTCATCGGCCAGGCACAGCCCACCTTCGGCTCGCACCATGGCGTACACCTCCTTGAGGTAATGTTCGGGCAAAAACACCTGCCCCGCCAC  >  NC_002947/3803193‑3803490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`