BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,944,909	G→A	5.6%	A317V (GCG→GTG)	PP_3478 ←	secretion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,944,909	0	G	A	5.6%	508.2 / 12.5	161	A317V (GCG→GTG)	PP_3478	secretion protein
Reads supporting (aligned to +/- strand): ref base G (75/77); new base A (4/5); total (79/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 165 total aligned reads displayed.

CATCGTCGCTCAGCACGCCGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCTGGTAG  >  NC_002947/3944760‑3945055

 aTCGTCGCTCAGCACGCCGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACcgc                                                                                                                                                   >  1:2341162/1‑150 (MQ=255)

    gtcgCTCAGCACGCCGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTc                                                                                                                                                >  1:668632/1‑150 (MQ=255)

      cgCTCAGCACGCCGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCg                                                                                                                                              <  2:2610831/150‑1 (MQ=255)

         tCAGCACGCCGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACg                                                                                                                                           >  2:1614769/1‑150 (MQ=255)

         tCAGCACGCCGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACg                                                                                                                                           >  2:1360764/1‑150 (MQ=255)

               cGCCGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTcgc                                                                                                                                     <  2:1653387/150‑1 (MQ=255)

                gCCGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTcgcg                                                                                                                                    >  1:3967137/1‑150 (MQ=255)

                gCCGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTcgcg                                                                                                                                    >  1:4121639/1‑150 (MQ=255)

                  cGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCGACACGTCGGACGCGGTGCGCa                                                                                                                                  <  2:491254/150‑1 (MQ=255)

                  cGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCa                                                                                                                                  >  1:5061632/1‑150 (MQ=255)

                    acggTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATg                                                                                                                                <  2:3486515/148‑1 (MQ=255)

                    aaGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATg                                                                                                                                <  2:128354/150‑1 (MQ=255)

                       gtgtTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTc                                                                                                                             <  2:3034395/150‑1 (MQ=255)

                          ttGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAgg                                                                                                                          <  2:1132492/150‑1 (MQ=255)

                          ttGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAgg                                                                                                                          <  1:2268803/150‑1 (MQ=255)

                          ttGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAgg                                                                                                                          <  2:3499042/150‑1 (MQ=255)

                              gCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGgtgt                                                                                                                      >  1:2340300/1‑150 (MQ=255)

                              gCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGgtgt                                                                                                                      >  1:530617/1‑150 (MQ=255)

                               ccATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGtt                                                                                                                     >  2:1795476/1‑150 (MQ=255)

                               ccATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGtt                                                                                                                     <  1:2744668/150‑1 (MQ=255)

                                  ttGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAg                                                                                                                  <  2:599048/150‑1 (MQ=255)

                                  ttGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAg                                                                                                                  <  1:4800873/150‑1 (MQ=255)

                                   tGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAgg                                                                                                                 <  1:1957877/150‑1 (MQ=255)

                                   tGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAgg                                                                                                                 <  2:3628460/150‑1 (MQ=255)

                                   tGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAgg                                                                                                                 >  2:361988/1‑150 (MQ=255)

                                        gCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTc                                                                                                            >  1:2367266/1‑150 (MQ=255)

                                          cAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGt                                                                                                          >  1:4204218/1‑150 (MQ=255)

                                              gTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAAc                                                                                                      >  2:2805052/1‑150 (MQ=255)

                                              gTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAAc                                                                                                      <  1:1585433/150‑1 (MQ=255)

                                                 gAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGaa                                                                                                   <  2:3360358/150‑1 (MQ=255)

                                                   ggATGTGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAaca                                                                                                 <  2:2688308/150‑1 (MQ=255)

                                                    gATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAacac                                                                                                >  2:4884163/1‑150 (MQ=255)

                                                    gATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAacac                                                                                                <  1:1617219/150‑1 (MQ=255)

                                                       gcgcGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTc                                                                                             <  2:3759275/150‑1 (MQ=255)

                                                       gcgcGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGGGTCGCGCAGGGTCCGGGTGTTGAGGCGCTCGTCAACGAACACGTc                                                                                             >  2:1768561/1‑150 (MQ=255)

                                                        atggAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTcg                                                                                            >  2:4173280/4‑150 (MQ=255)

                                                           gAGGTGGCCTCCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCgg                                                                                         <  1:1845287/150‑1 (MQ=255)

                                                           gAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCgg                                                                                         <  1:3688968/150‑1 (MQ=255)

                                                              gTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTc                                                                                      >  2:650953/1‑150 (MQ=255)

                                                                ggCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCtt                                                                                    <  1:346045/150‑1 (MQ=255)

                                                                 gCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTg                                                                                   >  1:3142767/1‑150 (MQ=255)

                                                                 gCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTg                                                                                   >  1:3143201/1‑150 (MQ=255)

                                                                 gCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTg                                                                                   <  2:668632/150‑1 (MQ=255)

                                                                  ccACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGa                                                                                  <  1:4827766/150‑1 (MQ=255)

                                                                  ccACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGa                                                                                  >  1:4506821/1‑150 (MQ=255)

                                                                  ccACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGa                                                                                  >  1:261440/1‑150 (MQ=255)

                                                                  ccACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGa                                                                                  <  2:3902137/150‑1 (MQ=255)

                                                                   cACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGGTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAg                                                                                 <  2:1733403/150‑1 (MQ=255)

                                                                       tCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATg                                                                             <  1:4655270/150‑1 (MQ=255)

                                                                          aTCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTc                                                                          >  1:2657184/1‑150 (MQ=255)

                                                                            cACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCtt                                                                        <  1:4025944/150‑1 (MQ=255)

                                                                            cACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCtt                                                                        <  1:1216511/150‑1 (MQ=255)

                                                                             aCCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTTAGCATGGTCttt                                                                       >  1:2135676/1‑149 (MQ=255)

                                                                              ccTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGa                                                                      <  1:1420589/150‑1 (MQ=255)

                                                                              ccTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGa                                                                      <  2:2389545/150‑1 (MQ=255)

                                                                              ccTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGa                                                                      <  2:2063079/150‑1 (MQ=255)

                                                                              ccTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGa                                                                      >  1:3562515/1‑150 (MQ=255)

                                                                               cTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGAc                                                                     >  1:1482416/1‑150 (MQ=255)

                                                                               cTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGAc                                                                     >  1:1032816/1‑150 (MQ=255)

                                                                               cTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGAc                                                                     <  2:3841299/150‑1 (MQ=255)

                                                                                tCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGAcc                                                                    >  2:3977918/1‑150 (MQ=255)

                                                                                tCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGAcc                                                                    >  2:2104541/1‑150 (MQ=255)

                                                                                 ccACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCa                                                                   >  2:2305297/1‑150 (MQ=255)

                                                                                 ccACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCa                                                                   >  1:2542209/1‑150 (MQ=255)

                                                                                    cTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGt                                                                <  2:306024/150‑1 (MQ=255)

                                                                                    cTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGt                                                                <  2:305579/150‑1 (MQ=255)

                                                                                      tCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTc                                                              <  2:1837018/150‑1 (MQ=255)

                                                                                         aGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAAc                                                           >  1:754139/1‑150 (MQ=255)

                                                                                         aGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAAc                                                           <  1:1562207/150‑1 (MQ=255)

                                                                                           caccacCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAAcgc                                                         >  2:1299902/1‑150 (MQ=255)

                                                                                           caccacCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAAcgc                                                         >  1:4961068/1‑150 (MQ=255)

                                                                                            accaccTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAAcgcg                                                        >  1:3844293/1‑150 (MQ=255)

                                                                                                    ggggTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGc                                                <  1:3277644/150‑1 (MQ=255)

                                                                                                    ggggTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGc                                                <  1:4249407/150‑1 (MQ=255)

                                                                                                     gggTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCg                                               <  1:3501204/150‑1 (MQ=255)

                                                                                                       gTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTc                                             >  2:3670963/1‑150 (MQ=255)

                                                                                                        ttGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCg                                            >  1:2482296/1‑150 (MQ=255)

                                                                                                            aCTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGc                                        >  2:688279/1‑150 (MQ=255)

                                                                                                              tGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTg                                      >  1:3417460/1‑150 (MQ=255)

                                                                                                               ggTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTgg                                     <  1:4513889/150‑1 (MQ=255)

                                                                                                                     gCGACTGCTGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGt                               <  2:89448/150‑1 (MQ=255)

                                                                                                                     gCGACTGCAGCAGCGTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGt                               <  2:1738664/150‑1 (MQ=255)

                                                                                                                      cGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTa                              <  1:1219934/150‑1 (MQ=255)

                                                                                                                        aCTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGa                            >  1:2295324/1‑150 (MQ=255)

                                                                                                                            cagcagCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTGAGGGTGTTGAGGCGCTCGTCATCGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGt                        <  2:3449572/150‑1 (MQ=255)

                                                                                                                              gcagcTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTgc                      <  2:1528511/150‑1 (MQ=255)

                                                                                                                               cagcTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTgcg                     >  2:3812292/1‑150 (MQ=255)

                                                                                                                                      ctgaccATGCGCGACACGTCGGACGCGGTGCGCATGGCCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGAcc              >  1:3324518/5‑150 (MQ=38)

                                                                                                                                       cGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCa             <  2:2355564/150‑1 (MQ=255)

                                                                                                                                       cGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCa             <  2:2355670/150‑1 (MQ=255)

                                                                                                                                           cATGCGCGACACGTCGGACGCGGTGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTc         >  2:1023391/1‑150 (MQ=255)

                                                                                                                                           cATGCGCGACACGTCGGACGCGGTGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTc         >  1:1989281/1‑150 (MQ=255)

                                                                                                                                            aTGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTcc        >  1:3770173/1‑150 (MQ=255)

                                                                                                                                            aTGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTcc        <  2:4699130/150‑1 (MQ=255)

                                                                                                                                             tGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCt       <  1:227628/150‑1 (MQ=255)

                                                                                                                                               cgcACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCTgg     <  1:3725715/150‑1 (MQ=255)

                                                                                                                                               cgcACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCTgg     <  1:1614769/150‑1 (MQ=255)

                                                                                                                                                gcACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCTGGt    >  2:4530829/1‑150 (MQ=255)

                                                                                                                                                 cACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCTGGTa   <  1:1033411/150‑1 (MQ=255)

                                                                                                                                                  aCCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCTGGTAg  >  2:3216615/1‑150 (MQ=255)

CATCGTCGCTCAGCACGCCGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCTGGTAG  >  NC_002947/3944760‑3945055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`