Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,975,806 C→T 5.5% E211E (GAG→GAA)  PP_3503 ← sigma‑54 dependent transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,975,8060CT 5.5% 505.7 / 12.4 162E211E (GAG→GAA) PP_3503sigma‑54 dependent transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (76/77);  new base T (7/2);  total (83/79)
Fisher's exact test for biased strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

Only 100 of 168 total aligned reads displayed.

CGCTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGTGCCGCTCTCCCCGCGGATCAGCA  >  NC_002947/3975657‑3975954
                                                                                                                                                     |                                                                                                                                                    
cGCTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTtc                                                                                                                                                      >  2:2538998/1‑150 (MQ=255)
 gCTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTtct                                                                                                                                                     <  1:3458948/150‑1 (MQ=255)
 gCTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTtct                                                                                                                                                     >  2:3485753/1‑150 (MQ=255)
   tCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCa                                                                                                                                                   >  2:2124629/1‑150 (MQ=255)
    cGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCAt                                                                                                                                                  <  1:347065/150‑1 (MQ=255)
               tcCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGGACAGt                                                                                                                                       >  2:4501436/1‑150 (MQ=255)
               tcCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGt                                                                                                                                       >  1:856875/1‑150 (MQ=255)
                ccTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGtt                                                                                                                                      >  2:3756960/1‑150 (MQ=255)
                ccTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGtt                                                                                                                                      >  1:4562792/1‑150 (MQ=255)
                ccTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGtt                                                                                                                                      >  1:936051/1‑150 (MQ=255)
                 cTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCATTCTCATGGCCGAACAGTTc                                                                                                                                     >  1:1981135/1‑150 (MQ=255)
                  tGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCg                                                                                                                                    >  1:782007/1‑150 (MQ=255)
                  tGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCg                                                                                                                                    >  1:2638464/1‑150 (MQ=255)
                  tGCAACACACGCAGCAAGCGCGTCTGCGCCTCCATTGGCATATCGCCGATTTCATCAAGAAACAGCGTGCCGCCATTGGCTTGTTCAAAACGTCCGCTTGAACGGTTCTGTGCGCCAGTAAAGGCACCCTTTTCATGGCCGAACAATTCg                                                                                                                                    >  1:2124354/1‑150 (MQ=255)
                  tGCAACACACGCAGCAAGCGCGTCTGCGCCTCCATTGGCATATCGCCGATTTCATCAAGAAACAGCGTGCCGCCATTGGCTTGTTCAAAACGTCCGCTTGAACGGTTCTGTGCGCCAGTAAAGGCACCCTTTTCATGGCCGAACAATTCg                                                                                                                                    >  1:2124301/1‑150 (MQ=255)
                  tGCAACACACGCAGCAAGCGCGTCTGCGCCTCCATTGGCATATCGCCGATTTCATCAAGAAACAGCGTGCCGCCATTGGCTTGTTCAAAACGTCCGCTTGAACGGTTCTGTGCGCCAGTAAAGGCACCCTTTTCATGGCCGAACAATTCg                                                                                                                                    >  2:437604/1‑150 (MQ=255)
                  tGCAACACACGCAGCAAGCGCGTCTGCGCCTCCATTGGCATATCGCCGATTTCATCAAGAAACAGCGTGCCGCCATTGGCTTGTTCAAAACGTCCGCTTGAACGGTTCTGTGCGCCAGTAAAGGCACCCTTTTCATGGCCGAACAATTCg                                                                                                                                    >  2:338925/1‑150 (MQ=255)
                     aacacaCGCAGCAAGCGCGTCTGCGCCTCCATTGGCATATCGCCGATTTCATCAAGAAACAGCGTGCCGCCATTGGCTTGTTCAAAACGTCCGCTTGAACGGTTCTGTGCGCCAGTAAAGGCACCCTTTTCATGGCCGAACAATTCGGAc                                                                                                                                 <  1:2823338/145‑1 (MQ=255)
                     aacacaCGCAGCAAGCGCGTCTGCGCCTCCATTGGCATATCGCCGATTTCATCAAGAAACAGCGTGCCGCCATTGGCTTGTTCAAAACGTCCGCTTGAACGGTTCTGTGCGCCAGTAAAGGCACCCTTTTCATGGCCGAACAATTCGGAc                                                                                                                                 <  1:1335731/145‑1 (MQ=255)
                     aaGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGAc                                                                                                                                 <  1:1078259/150‑1 (MQ=255)
                     aaGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGAc                                                                                                                                 <  2:1548444/150‑1 (MQ=255)
                      aGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACt                                                                                                                                <  1:218407/150‑1 (MQ=255)
                       gAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTg                                                                                                                               >  1:249167/1‑150 (MQ=255)
                       gAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTg                                                                                                                               >  1:3047464/1‑150 (MQ=255)
                         aaCGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGa                                                                                                                             >  1:3494680/1‑150 (MQ=255)
                            gcagcaAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCa                                                                                                                          >  1:2419573/1‑150 (MQ=255)
                             cagcaAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCaa                                                                                                                         <  2:1968957/150‑1 (MQ=255)
                              agcaAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAg                                                                                                                        <  1:131102/150‑1 (MQ=255)
                              agcaAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAg                                                                                                                        >  1:3506049/1‑150 (MQ=255)
                              agcaAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAg                                                                                                                        >  1:1907044/1‑150 (MQ=255)
                              agcaAGATGCCCAGCAATACAAACGGCAGCTCGCCGAATTCATCAAGAAACAAAGAGCCGCCAACGGCCGCTTCGATACGCCCGGACTAGCGCTGGTCAGCACAGCTAAAGGCGCCCTTCACAAGGCCGAACAGATCGCACAGGAACAAg                                                                                                                        >  2:2654712/1‑150 (MQ=255)
                                 aaGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGc                                                                                                                     >  1:4822096/1‑150 (MQ=255)
                                  aGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCt                                                                                                                    >  1:669605/1‑150 (MQ=255)
                                   gTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTc                                                                                                                   <  2:930780/150‑1 (MQ=255)
                                    ttGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCg                                                                                                                  >  1:3114448/1‑150 (MQ=255)
                                    ttGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCg                                                                                                                  <  2:1756825/150‑1 (MQ=255)
                                          tGCAATTCCAACGGCAGGTCGCCGATTTCTTCAGGTTAGAATGTGCCGCCAGCGGCCGCTTCGTTTCGCCGGGTCGTGCGCTGGTGAGCACCGGTCGTGGCGCCCGGCGCATGGCCGTACGGTGCGGACTGGATGGGGTGCTCggggggc                                                                                                            >  2:720779/1‑147 (MQ=255)
                                          tGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATc                                                                                                            <  2:2784241/150‑1 (MQ=255)
                                          tGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATc                                                                                                            >  1:1797962/1‑150 (MQ=255)
                                           gCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCACGGTCTTGTGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGCTCg                                                                                                           >  2:4413895/1‑150 (MQ=255)
                                                  cAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCa                                                                                                    >  1:2845015/1‑150 (MQ=255)
                                                  cAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCa                                                                                                    <  2:1229768/150‑1 (MQ=255)
                                                         aGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATc                                                                                             <  1:1330020/150‑1 (MQ=255)
                                                         aGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATc                                                                                             <  1:4848736/150‑1 (MQ=255)
                                                           gTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGATCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATcgc                                                                                           <  2:133791/150‑1 (MQ=255)
                                                             cGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGa                                                                                         <  1:1491825/150‑1 (MQ=255)
                                                             cGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGa                                                                                         <  2:1431586/150‑1 (MQ=255)
                                                              gCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGAt                                                                                        >  2:3214659/1‑150 (MQ=255)
                                                               ccGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATg                                                                                       >  2:2217996/1‑150 (MQ=255)
                                                                cGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGa                                                                                      >  2:1775868/1‑150 (MQ=255)
                                                                cGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGa                                                                                      >  2:3567119/1‑150 (MQ=255)
                                                                  aTTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAAc                                                                                    >  1:2579043/1‑150 (MQ=255)
                                                                    ttCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACgg                                                                                  <  2:3263917/150‑1 (MQ=255)
                                                                      catcaAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGgct                                                                                <  1:4149267/150‑1 (MQ=255)
                                                                      catcaAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGgct                                                                                <  1:1286386/150‑1 (MQ=255)
                                                                       atcaAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGgctg                                                                               <  1:4167076/150‑1 (MQ=255)
                                                                       atcaAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTAAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGgctg                                                                               >  1:61696/1‑150 (MQ=255)
                                                                        tcaAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGgctgc                                                                              >  1:2923759/1‑150 (MQ=255)
                                                                        tcaAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGgctgc                                                                              >  2:1103009/1‑150 (MQ=255)
                                                                        tcaAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGgctgc                                                                              >  1:2553581/1‑150 (MQ=255)
                                                                          aaGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTc                                                                            <  1:3778946/150‑1 (MQ=255)
                                                                                cAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCg                                                                      <  1:3756960/150‑1 (MQ=255)
                                                                                 aaTGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGt                                                                     >  2:1023593/1‑150 (MQ=255)
                                                                                  ctgtgCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCGGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGtt                                                                    <  2:3868219/149‑1 (MQ=255)
                                                                                    gtgCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGc                                                                  >  2:3754514/1‑150 (MQ=255)
                                                                                              cGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGt                                                        <  1:1573875/150‑1 (MQ=255)
                                                                                                   gCTTCGATACGCCCGGTCTTGCGCTGGTGTGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCTGTTGTTCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAg                                                   >  1:3590835/1‑150 (MQ=255)
                                                                                                   gCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAg                                                   <  1:3908569/150‑1 (MQ=255)
                                                                                                   gCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAg                                                   >  1:913220/1‑150 (MQ=255)
                                                                                                    cTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAgg                                                  <  2:1080147/150‑1 (MQ=255)
                                                                                                     ttCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGt                                                 >  2:3327884/1‑150 (MQ=255)
                                                                                                     ttCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGt                                                 >  2:2621267/1‑150 (MQ=255)
                                                                                                     ttCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGt                                                 >  1:4609705/1‑150 (MQ=255)
                                                                                                        gATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTgcg                                              >  1:587447/1‑150 (MQ=255)
                                                                                                         aTACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTgcgc                                             <  1:4093880/150‑1 (MQ=255)
                                                                                                          tACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTgcgcg                                            >  2:1416566/1‑150 (MQ=255)
                                                                                                             gCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCATTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGcca                                         <  2:1981135/150‑1 (MQ=255)
                                                                                                              cccGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGccac                                        >  2:3789802/1‑150 (MQ=255)
                                                                                                                cGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGccacca                                      <  2:1810332/150‑1 (MQ=255)
                                                                                                                cGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGccacca                                      >  1:3886205/1‑150 (MQ=255)
                                                                                                                     ttGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGctct                                 >  1:2654426/1‑150 (MQ=255)
                                                                                                                      tGCGCTGGTGAGCACCGGTAAAGGCGACCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCtt                                <  2:1481102/150‑1 (MQ=255)
                                                                                                                         gcTGGTGAGCACCGGTAAAGGCGCCCTTCTCCTGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGc                             <  2:4285380/150‑1 (MQ=255)
                                                                                                                         gcTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGc                             <  1:1041606/150‑1 (MQ=255)
                                                                                                                             gTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGt                         <  2:938817/150‑1 (MQ=255)
                                                                                                                             gTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGt                         >  2:4554276/1‑150 (MQ=255)
                                                                                                                               gAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGTGc                       <  1:1163717/150‑1 (MQ=255)
                                                                                                                               gAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGTGc                       <  1:3096815/150‑1 (MQ=255)
                                                                                                                                 gCACCGGTAAAGGCGCCCTTCTCCTGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGTGCCg                     <  2:2045493/150‑1 (MQ=255)
                                                                                                                                 gCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACCGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGTGCCg                     <  1:1218966/150‑1 (MQ=255)
                                                                                                                                  cACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGTGCCGc                    >  2:4095650/1‑150 (MQ=255)
                                                                                                                                  cACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGTGCCGc                    >  1:1711235/1‑150 (MQ=255)
                                                                                                                                      ggTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGTGCCGCtctc                >  2:544526/1‑150 (MQ=255)
                                                                                                                                       gTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGTGCCGCTCTcc               >  2:2413087/1‑150 (MQ=255)
                                                                                                                                       gTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGTGCCGCTCTcc               >  2:4857710/1‑150 (MQ=255)
                                                                                                                                             gcgcCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGTGCCGCTCTCCCCGCgg         >  1:1879441/1‑150 (MQ=255)
                                                                                                                                                 ccTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGTGCCGCTCTCCCCGCGGATCa     <  2:3840975/150‑1 (MQ=255)
                                                                                                                                                 ccTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGTGCCGCTCTCCCCGCGGATCa     <  1:3485753/150‑1 (MQ=255)
                                                                                                                                                    tctcATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGTGCCGCTCTCCCCGCGGATCAGca  <  1:2538668/150‑1 (MQ=255)
                                                                                                                                                    tctcATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGTGCCGCTCTCCCCGCGGATCAGca  <  1:908825/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGTGCCGCTCTCCCCGCGGATCAGCA  >  NC_002947/3975657‑3975954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑