BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,433,238	C→A	5.2%	Q348K (CAG→AAG)	PP_3925 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,433,238	0	C	A	5.2%	644.9 / 16.4	192	Q348K (CAG→AAG)	PP_3925	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (100/82); new base A (5/5); total (105/87)
Fisher's exact test for biased strand distribution p-value = 7.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 195 total aligned reads displayed.

ACAACTTCGATTACTACTATTGCTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCA  >  NC_002947/4433089‑4433386

aCAACTTCGATTACTACTATTGCTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATCGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGc                                                                                                                                                      <  2:4393965/150‑1 (MQ=255)

 cAACTTCGATTACTACTATTGCTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCa                                                                                                                                                     <  1:929076/150‑1 (MQ=255)

 cAACTTCGATTACTACTATTGCTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCa                                                                                                                                                     <  1:662556/150‑1 (MQ=255)

  aaCTTCGATTACTACTATTGCTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAg                                                                                                                                                    <  2:76759/150‑1 (MQ=255)

  aaCTTCGATTACTACTATTGCTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAg                                                                                                                                                    <  2:4236965/150‑1 (MQ=255)

     ttCGATTACTACTATTGCTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTc                                                                                                                                                 >  1:1682897/1‑150 (MQ=255)

        gATTACTACTATTGCTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTGCAAGGTCAtt                                                                                                                                              <  1:3148924/150‑1 (MQ=255)

        gATTACTACTATTGCTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCACCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTGCAAGGTCAtt                                                                                                                                              <  1:1806893/150‑1 (MQ=255)

        gATTACTACTATTGCTAGGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTGCAAGGTCAtt                                                                                                                                              <  1:4621766/150‑1 (MQ=255)

         aTTACTACTATTGCTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCGAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCAttt                                                                                                                                             <  1:2062294/150‑1 (MQ=255)

         aTTACTACTATTGCTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCAttt                                                                                                                                             <  2:1809163/150‑1 (MQ=255)

          ttACTACTATTGCTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTc                                                                                                                                            <  1:3646964/150‑1 (MQ=255)

          ttACTACTATTGCTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTGCAAGGTCAtttg                                                                                                                                            <  2:3939044/150‑2 (MQ=255)

             ctactaTTGCTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTccga                                                                                                                                         <  2:2362950/150‑3 (MQ=255)

             ctactaTTGCTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCaa                                                                                                                                         <  1:4159724/150‑1 (MQ=255)

             ctactaTTGCTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCaa                                                                                                                                         <  2:655207/150‑1 (MQ=255)

             ctactaTTGCTATGGCCTGTTCCGCCTGGCAGACATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCaa                                                                                                                                         <  1:2076376/150‑1 (MQ=255)

                   ttGCTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAg                                                                                                                                   >  2:3421277/1‑150 (MQ=255)

                    tGCGATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCCTGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGc                                                                                                                                  <  2:1725204/150‑1 (MQ=255)

                      cTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCct                                                                                                                                >  1:1050206/1‑150 (MQ=255)

                      cTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCct                                                                                                                                >  1:2387603/1‑150 (MQ=255)

                       tATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCctc                                                                                                                               <  1:612927/150‑1 (MQ=255)

                          ggCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGa                                                                                                                            <  2:612061/150‑1 (MQ=255)

                          ggCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGa                                                                                                                            <  1:3938019/150‑1 (MQ=255)

                          ggCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGa                                                                                                                            <  2:3020195/150‑1 (MQ=255)

                          ggCCTGGTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGa                                                                                                                            <  1:3821417/150‑1 (MQ=255)

                           gCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAg                                                                                                                           >  1:3495970/1‑150 (MQ=255)

                               gTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGcaca                                                                                                                       <  2:919732/150‑1 (MQ=255)

                                 ttgtccTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGc                                                                                                                     >  2:1961103/5‑150 (MQ=255)

                                       tGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCacga                                                                                                               >  2:1978983/1‑150 (MQ=255)

                                            ggcaTCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCacgacgacg                                                                                                          >  1:3453342/1‑150 (MQ=255)

                                            ggcaTCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCacgacgacg                                                                                                          <  2:5092317/150‑1 (MQ=255)

                                            ggcaTCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCacgacgacg                                                                                                          <  1:4208714/150‑1 (MQ=255)

                                             gcaTCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTc                                                                                                                                            >  1:2535412/1‑115 (MQ=255)

                                             gcaTCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTc                                                                                                                                            <  2:2535412/115‑1 (MQ=255)

                                                tCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATa                                                                                                      >  1:1601211/1‑150 (MQ=255)

                                                   ttCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACa                                                                                                   >  2:4106792/1‑150 (MQ=255)

                                                        cagATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGaa                                                                                              >  2:2456751/1‑150 (MQ=255)

                                                          gATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGaaaa                                                                                            <  1:701148/150‑1 (MQ=255)

                                                            tCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACa                                                                                          >  2:444362/1‑150 (MQ=255)

                                                              tactacCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGc                                                                                        <  2:2291028/150‑1 (MQ=255)

                                                               actacCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGCTAACAAGGAAAACAGCa                                                                                       >  2:3575419/1‑150 (MQ=255)

                                                                ctacCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCAt                                                                                      >  2:2850388/1‑150 (MQ=255)

                                                                  acCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGt                                                                                    >  2:5072338/1‑150 (MQ=255)

                                                                    cGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAg                                                                                                                      >  2:3317890/1‑114 (MQ=255)

                                                                      ctactaCCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCaa                                                                                >  2:1471277/1‑150 (MQ=255)

                                                                          tacCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGAcc                                                                            >  2:1546122/1‑150 (MQ=255)

                                                                                ggCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTg                                                                      >  1:4035640/1‑150 (MQ=255)

                                                                                ggCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTg                                                                      >  1:3025539/1‑150 (MQ=255)

                                                                                  ccAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGtt                                                                    >  1:5069902/1‑150 (MQ=255)

                                                                                  ccAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGtt                                                                    <  1:2007970/150‑1 (MQ=255)

                                                                                   cAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTc                                                                   <  1:4113717/150‑1 (MQ=255)

                                                                                        ccAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTcc                                                                                                                                           >  2:1861141/1‑73 (MQ=255)

                                                                                        ccAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCt                                                              >  2:4227466/1‑150 (MQ=255)

                                                                                        ccAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCt                                                              >  2:947053/1‑150 (MQ=255)

                                                                                         cAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTc                                                             >  2:1704430/1‑150 (MQ=255)

                                                                                                aGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCa                                                      <  1:1177676/150‑1 (MQ=255)

                                                                                                  cGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAg                                                    >  1:1909906/1‑150 (MQ=255)

                                                                                                   gTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGa                                                   <  2:431819/150‑1 (MQ=255)

                                                                                                    tttCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGAt                                                  >  2:3298836/1‑150 (MQ=255)

                                                                                                        gCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGcc                                              <  1:4777706/150‑1 (MQ=255)

                                                                                                           cAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCttt                                           >  1:1153371/1‑150 (MQ=255)

                                                                                                           cAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCttt                                           <  1:3861452/150‑1 (MQ=255)

                                                                                                           cAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCttt                                           >  1:2234393/1‑150 (MQ=255)

                                                                                                           cAGGTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCttt                                           <  2:2694326/150‑1 (MQ=255)

                                                                                                               tcattcaCATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGttt                                       >  2:2299998/1‑150 (MQ=255)

                                                                                                                 attcaCATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTcc                                     >  2:3781851/1‑150 (MQ=255)

                                                                                                                 attcaCATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTcc                                     >  1:458722/1‑150 (MQ=255)

                                                                                                                  ttcaCATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCg                                    >  1:5110635/1‑150 (MQ=255)

                                                                                                                  ttcaCATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCCGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCg                                    >  1:4241902/1‑150 (MQ=255)

                                                                                                                     acaTGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGcc                                              >  1:4311707/1‑137 (MQ=255)

                                                                                                                     acaTGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGgcg                                 >  2:4942722/1‑150 (MQ=255)

                                                                                                                     acaTGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCAGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATAGCCTGTGTTACCGgcg                                 >  2:573584/1‑150 (MQ=255)

                                                                                                                       aTGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGcc                               >  1:3072452/1‑150 (MQ=255)

                                                                                                                              gCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTg                        <  2:2779818/150‑1 (MQ=255)

                                                                                                                               cTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTgg                       <  1:3657507/150‑1 (MQ=255)

                                                                                                                                ttGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGAccc                                                                           >  2:371825/1‑97 (MQ=255)

                                                                                                                                ttGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGAccc                                                                           <  1:371825/97‑1 (MQ=255)

                                                                                                                                  gCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGCGGCAt                    <  2:1102050/150‑1 (MQ=255)

                                                                                                                                    tGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCg                  >  2:3849377/1‑150 (MQ=255)

                                                                                                                                    tGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCg                  >  1:5020944/1‑150 (MQ=255)

                                                                                                                                      ttggaaaTGACCTGCAAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGc                >  2:4116309/5‑150 (MQ=255)

                                                                                                                                      gAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGc                >  1:282470/1‑150 (MQ=255)

                                                                                                                                      gAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGc                <  1:534904/150‑1 (MQ=255)

                                                                                                                                        aCAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGa              <  2:1289225/150‑1 (MQ=255)

                                                                                                                                        aCAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGa              <  2:4507076/150‑1 (MQ=255)

                                                                                                                                         cAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAg             <  2:2205374/150‑1 (MQ=255)

                                                                                                                                          aaaTGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAgg            <  1:1497322/150‑1 (MQ=255)

                                                                                                                                          aaaTGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAgg            >  2:1418138/1‑150 (MQ=255)

                                                                                                                                          aaaTGACCTGCAAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAgg            >  1:3936259/1‑150 (MQ=255)

                                                                                                                                          aaaTGACCTGCAAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAgg            >  2:4613668/1‑150 (MQ=255)

                                                                                                                                           aaTGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGc           >  2:4852995/1‑150 (MQ=255)

                                                                                                                                           aaTGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGc           >  2:3943260/1‑150 (MQ=255)

                                                                                                                                           aaTGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGc           >  2:216124/1‑150 (MQ=255)

                                                                                                                                           aaTGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGc           >  2:1363475/1‑150 (MQ=255)

                                                                                                                                           aaTGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGc           >  2:1679926/1‑150 (MQ=255)

                                                                                                                                            aTGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGcc          >  1:1452408/1‑150 (MQ=255)

                                                                                                                                                ccTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCg      >  2:2975698/1‑150 (MQ=255)

                                                                                                                                                ccTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCg      <  2:537235/150‑1 (MQ=255)

                                                                                                                                                    gCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCa  >  1:1890665/1‑150 (MQ=255)

ACAACTTCGATTACTACTATTGCTATGGCCTGTTCCGCCTGGCAGGCATCGTTCAGCAGATCTACTACCGCTACTACCATGGCCAGACCCAGGACAAGCGTTTCGCCCAGTTCATTCACATGAACCGCTTGCTGGAACAAATGACCTTGCAGGTCATTTCCAAGTCCAGCCTCTGAGCACAGCCCACGACGACGGATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCA  >  NC_002947/4433089‑4433386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`