Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,588,037 G→C 17.3% intergenic (+154/+63) PP_4061 → / ← PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,588,0370GC17.3% 292.9 / 49.8 110intergenic (+154/+63)PP_4061/PP_4063hypothetical protein/long‑chain fatty acid‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base G (22/69);  new base C (2/17);  total (24/86)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

Only 100 of 110 total aligned reads displayed.

ACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGT  >  NC_002947/4587890‑4588185
                                                                                                                                                   |                                                                                                                                                      
aCCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGaa                                                                                                                                                      <  2:4504664/150‑1 (MQ=255)
  gatgaccgtgaccggcaaggtgcagaagttccgcatgcgtgagatcagtgtggcggagattgcggctgcatctgcgggttgatgtgctggcgcGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATg                                                                                                                                                    <  1:1396320‑M1/57‑1 (MQ=255)
   aCGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCATCGCGGGTAAAACCCCACCTACCAGGGCCCGAGCCGGCCATGAAAgc                                                                                                                                                  >  2:4858902/1‑150 (MQ=255)
    cGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcg                                                                                                                                                <  2:793603/150‑1 (MQ=255)
       ccgtgaccggcaaggtgcagaagttccgcatgcgtgagatcagtgtggcggagattgcggctgcatctgcgggttgatgtgctggcgcGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGCGCCGGCCTTGAATgcggcg                                                                                                                                             <  2:2174155‑M1/62‑1 (MQ=255)
       ccgtgaccggcaaggtgcagaagttccgcatgcgtgagatcagtgtggcggagattgcggctgcatctgcgggttgatgtgctggcgcGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGCGCCGGCCTTGAATgcggcg                                                                                                                                             <  2:2174108‑M1/62‑1 (MQ=255)
         ccGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCt                                                                                                                                           <  1:3774792/150‑1 (MQ=255)
           gaccggcaaggtgcagaagttccgcatgcgtgagatcagtgtggcggagattgcggctgcatctgcgggttgatgtgctggcgcGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGc                                                                                                                                         >  1:2105013‑M1/85‑150 (MQ=255)
            gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGACGCTGcc                                                                                                                                        <  1:1799622/150‑1 (MQ=255)
              gTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGcccc                                                                                                                                      <  1:4365459/150‑1 (MQ=255)
              cgtcaaggtgcagaagttccgcatgcgtgagatcagtgtggcggagattgcggctgcatctgcgggttgatgtgctggcgcGTCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGcccc                                                                                                                                      >  2:3233053‑M1/82‑150 (MQ=255)
               tGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCt                                                                                                                                     <  2:158580/150‑1 (MQ=255)
               tGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCt                                                                                                                                     <  2:4839667/150‑1 (MQ=255)
               ggcaaggtgcagaagttccgcatgcgtgagatcagtgtggcggagattgcggctgcatctgcgggttgatgtgctggcgcGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCt                                                                                                                                     >  1:2660638‑M1/81‑150 (MQ=255)
                gcaaggtgcagaagttccgcatgcgtgagatcagtgtggcggagattgcggctgcatctgcgggttgatgtgctggcgcGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTg                                                                                                                                    >  1:3839755‑M1/80‑150 (MQ=255)
                gcaaggtgcagaagttccgcatgcgtgagatcagtgtggcggagattgcggctgcatctgcgggttgatgtgctggcgcGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTg                                                                                                                                    >  2:4908141‑M1/80‑150 (MQ=255)
                gcaaggtgcagaagttccgcatgcgtgagatcagtgtggcggagattgcggctgcatctgcgggttgatgtgctggcgcGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTg                                                                                                                                    >  2:1396320‑M1/80‑150 (MQ=255)
                gAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTg                                                                                                                                    <  1:761054/150‑1 (MQ=255)
                gAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTACTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTg                                                                                                                                    <  2:672680/150‑1 (MQ=255)
                 aaCAGCTGATCTGCGCTGCTCCATTGAACGGTTGAGGATGACCGGGTGCTTGGCACGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTgg                                                                                                                                   <  2:3882714/150‑1 (MQ=255)
                  aCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGt                                                                                                                                  <  1:234233/150‑1 (MQ=255)
                  aCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGt                                                                                                                                  <  1:2792977/150‑1 (MQ=255)
                   cAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                                                                                                                                 <  2:1111077/150‑1 (MQ=255)
                   cAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCGGCCCCTGGTa                                                                                                                                 <  2:1110984/150‑1 (MQ=255)
                   aggtgcagaagttccgcatgcgtgagatcagtgtggcggagattgcggctgcatctgcgggttgatgtgctggcgcGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                                                                                                                                 >  2:729872‑M1/77‑150 (MQ=255)
                   aggtgcagaagttccgcatgcgtgagatcagtgtggcggagattgcggctgcatctgcgggttgatgtgctggcgcGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                                                                                                                                 >  1:2893873‑M1/77‑150 (MQ=255)
                   aggtgcagaagttccgcatgcgtgagatcagtgtggcggagattgcggctgcatctgcgggttgatgtgctggcgcGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                                                                                                                                 >  1:1893293‑M1/77‑150 (MQ=255)
                    aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAg                                                                                                                                <  1:2539770/150‑1 (MQ=255)
                    aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAg                                                                                                                                <  1:3060299/150‑1 (MQ=255)
                    aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAg                                                                                                                                <  1:3431943/150‑1 (MQ=255)
                     gCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGGCAGCGCCGCATTCAAGGCCGGCACGGTCCCTGGTAgg                                                                                                                               <  2:3774107/150‑1 (MQ=18)
                      cTGATCTGCGCTGCTCCATTGACAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGa                                                                                                                              <  2:3769493/150‑1 (MQ=255)
                      cTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGa                                                                                                                              <  1:1407183/150‑1 (MQ=255)
                       tGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAg                                                                                                                             <  1:1066915/150‑1 (MQ=255)
                          tCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCACGGTTAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg                                                                                                                          <  1:1550102/150‑1 (MQ=255)
                          tCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGTCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGGCAGCGCCGCATTCAAGGCCGGCACGGTCCCTGGTAGGAGCgg                                                                                                                          <  1:4977237/150‑1 (MQ=17)
                          tCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGGCAGCGCCGCATTCAAGGCCGGCACGGTCCCTGGTAGGAGCgg                                                                                                                          <  2:4508363/150‑1 (MQ=17)
                          tCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGGCAGCGCCGCATTCAAGGCCGGCACGGTCCCTGGTAGGAGCgg                                                                                                                          <  1:4976953/150‑1 (MQ=17)
                          tCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGGCAGCGCCGCATTCAAGGCCGGCACGGTCCCTGGTAGGAGCgg                                                                                                                          <  1:1846693/150‑1 (MQ=17)
                          tCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGGCAGCGCCGCATTCAAGGCCGGCACGGTCCCTGGTAGGAGCgg                                                                                                                          <  1:1139710/150‑1 (MQ=17)
                          tCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGGCAGCGCCGCATTCAAGGCCGGCACGGTCCCTGGTAGGAGCgg                                                                                                                          <  2:4130405/150‑1 (MQ=17)
                          tCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGGCAGCGCCGCATTCAAGGCCGGCACGGTCCCTGGTAGGAGCgg                                                                                                                          <  2:4130145/150‑1 (MQ=17)
                          tCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg                                                                                                                          <  2:2277161/150‑1 (MQ=255)
                          tCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg                                                                                                                          <  1:1118314/150‑1 (MQ=255)
                          tCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg                                                                                                                          <  2:4591986/150‑1 (MQ=255)
                          tCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg                                                                                                                          <  2:388578/150‑1 (MQ=255)
                          tCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg                                                                                                                          <  2:388435/150‑1 (MQ=255)
                          tCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg                                                                                                                          <  1:892813/150‑1 (MQ=255)
                          tCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg                                                                                                                          <  1:1615955/150‑1 (MQ=255)
                          tCTGCGCTGCTCCATTGAAAGGTTGAGGAGGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg                                                                                                                          <  2:4592124/150‑1 (MQ=255)
                           ctgctctgctCCATTGAAAGGTTTAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCggg                                                                                                                         <  2:3792185/150‑1 (MQ=255)
                           cTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGGCAGCGCCGCATTCAAGGCCGGCACGGTCCCTGGTAGGAGCggg                                                                                                                         <  1:1812703/150‑1 (MQ=17)
                             gcgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGtt                                                                                                                       <  2:3430588/150‑1 (MQ=255)
                                ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGGCAGCGCCGCATTCAAGGCCGGCACGGTCCCTGGTAGGAGCGGGTTTAc                                                                                                                    <  1:830049/150‑1 (MQ=17)
                                ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGATTAc                                                                                                                    <  1:2666674/150‑1 (MQ=255)
                                  gctCCCTTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTAccc                                                                                                                  <  2:4677201/150‑1 (MQ=255)
                                       cgtgagatcagtgtggcggagattgcggctgcatctgcgggttgatgtgctggcgcGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTCCCCGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTTGGAGCGGTTTTACCCGCGaa                                                                                                             >  2:1434238‑M1/57‑150 (MQ=255)
                                         tGAAAGTTGGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAACCCCGCTCCTACCCGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAaga                                                                                                           <  2:4033977/150‑1 (MQ=255)
                                           aaaGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGGCAGCGCCGCATTCAAGGCCGGCACGGTCCCTGGTAGGAGCGGGt                                                                                                                        <  1:2670780/135‑1 (MQ=14)
                                           aaaGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGGCAGCGCCGCATTCAAGGCCGGCACGGTCCCTGGTAGGAGCGGGt                                                                                                                        >  2:2670780/1‑135 (MQ=14)
                                                                               gATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg                                                                                                                          <  2:3188435/97‑1 (MQ=255)
                                                                                                        CTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCAc                                            <  2:2274102‑M1/150‑1 (MQ=255)
                                                                                                         TTCGCGGGTAAACCCGCTCCAACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCGGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAAACTCCGCCACACTGATCTCACg                                           <  2:601320‑M1/150‑1 (MQ=255)
                                                                                                            GCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCAt                                        <  2:4046631‑M1/150‑1 (MQ=255)
                                                                                                            GCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCAt                                        <  1:3403785‑M1/150‑1 (MQ=255)
                                                                                                                 TAAACCCGCTCCTACCAGGGGCAGCGCCGCATTCAAGGCCGGCACGGTCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGa                                   <  1:1143490‑M1/150‑1 (MQ=255)
                                                                                                                 TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCCGCGCTGCCCCTGGTAGGAGCGGGTGTACCCGCGAAGAGGCCGGCCGCGCCCGCACAGCAACCCGCAGATGCAGCCGCACTCTCCGCCACACAGAGCTCACGCAAGcggc                                   <  2:3483533‑M1/150‑2 (MQ=255)
                                                                                                                     CCCGCTCCTACCAGGGGCAGCGCCGCATTCAAGGCCGGCACGGTCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACtt                               <  2:166304‑M1/150‑1 (MQ=255)
                                                                                                                      CCGCTCCTACCAGGGGCAGCGCCGCATTCAAGGCCGGCACGGTCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTc                              <  1:547745‑M1/150‑1 (MQ=255)
                                                                                                                      CCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTc                              <  1:1353839‑M1/150‑1 (MQ=255)
                                                                                                                      CCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTc                              <  2:346374‑M1/150‑1 (MQ=255)
                                                                                                                        GCTCCTACCAGGGGCAGCGCCGCATTCAAGGCCGGCGCGGTCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTg                            <  1:2174108‑M1/150‑1 (MQ=255)
                                                                                                                        GCTCCTACCAGGGGCAGCGCCGCATTCAAGGCCGGCGCGGTCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTg                            <  1:2174155‑M1/150‑1 (MQ=255)
                                                                                                                         CTCCTACCAGGGGCAGCGCCGCATTCAAGGCCGGCACGGTCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGc                           <  2:1168260‑M1/150‑1 (MQ=255)
                                                                                                                         CTCCTACCAGGGGCAGCGCCGCATTCAAGGCCGGCACGGTCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGc                           >  2:547745‑M1/1‑150 (MQ=255)
                                                                                                                           CCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCAc                         <  1:2751197‑M1/150‑1 (MQ=255)
                                                                                                                             TACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCt                       <  2:2838044‑M1/150‑1 (MQ=255)
                                                                                                                             TACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCt                       <  1:132059‑M1/150‑1 (MQ=255)
                                                                                                                             TACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCt                       <  1:2472988‑M1/150‑1 (MQ=255)
                                                                                                                             TACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCt                       <  2:141748‑M1/150‑1 (MQ=255)
                                                                                                                             TACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCt                       <  1:3866828‑M1/150‑1 (MQ=255)
                                                                                                                             TACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCt                       <  2:142024‑M1/150‑1 (MQ=255)
                                                                                                                             TACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCt                       <  2:2342971‑M1/150‑1 (MQ=255)
                                                                                                                             TACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCt                       <  2:3767555‑M1/150‑1 (MQ=255)
                                                                                                                             TACCAGGGACCGTGACGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCt                       <  2:1107586‑M1/150‑1 (MQ=255)
                                                                                                                               CCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGGAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTg                     <  1:150855‑M1/150‑1 (MQ=255)
                                                                                                                                CAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGc                    <  1:347930‑M1/150‑1 (MQ=255)
                                                                                                                                 AGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGcc                   <  1:3785681‑M1/150‑1 (MQ=255)
                                                                                                                                  GGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCg                  <  2:4782108‑M1/150‑1 (MQ=255)
                                                                                                                                    GACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGt                <  2:1361209‑M1/150‑1 (MQ=255)
                                                                                                                                    GACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGt                <  2:1361184‑M1/150‑1 (MQ=255)
                                                                                                                                    GACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGt                <  2:1361041‑M1/150‑1 (MQ=255)
                                                                                                                                       cGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCcggtcac             >  1:3312913/1‑150 (MQ=255)
                                                                                                                                            cGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCcggtcacggtca        >  1:3786276/1‑150 (MQ=255)
                                                                                                                                            cGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCcggtcacggtca        >  1:698997/1‑150 (MQ=255)
                                                                                                                                            cGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCcggtcacggtca        >  2:2472410/1‑150 (MQ=255)
                                                                                                                                            cGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCcggtcacggtca        >  1:2544246/1‑150 (MQ=255)
                                                                                                                                               ccTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCg     >  2:446205/1‑150 (MQ=255)
                                                                                                                                                 ttGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCggg   >  1:2167843/1‑150 (MQ=255)
                                                                                                                                                  tGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGt  >  2:773890/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
ACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGT  >  NC_002947/4587890‑4588185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: