Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,591,694 C→T 13.7% R136R (CGC→CGT)  mccB → methylcrotonyl‑CoA carboxylase biotin‑containing subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,591,6940CT13.7% 546.5 / 64.0 212R136R (CGC→CGT) mccBmethylcrotonyl‑CoA carboxylase biotin‑containing subunit beta
Reads supporting (aligned to +/- strand):  ref base C (90/93);  new base T (11/18);  total (101/111)
Fisher's exact test for biased strand distribution p-value = 3.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

CCAGCGGCGGGCGTGATTGCCGGCATCGGCCGCGTGGAAGGCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGG  >  NC_002947/4591545‑4591842
                                                                                                                                                     |                                                                                                                                                    
ccAGCGGCGGGCGTGATTGCCGGCATCGGCCGCGTGGAAGGCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGc                                                                                                                                                      <  2:2139955/150‑1 (MQ=255)
      gcgggcgTGATTGCCGGCATCGGCCGCGTGGAAGGCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCg                                                                                                                                                >  1:52403/1‑150 (MQ=255)
          gcgTGATTGCCGGCATCGGCCGCGTGGAAGGCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCa                                                                                                                                            <  1:1060953/150‑1 (MQ=255)
          gcgTGATTGCCGGCATCGGCCGCGTGGAAGGCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCa                                                                                                                                            <  1:1060910/150‑1 (MQ=255)
          gcgTGATTGCCGGCATCGGCCGCGTGGAAGGCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCa                                                                                                                                            >  2:1668968/1‑150 (MQ=255)
            gTGATTGCCGGCATCGGCCGCGTGGAAGGCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGCGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCatc                                                                                                                                          >  2:958030/1‑150 (MQ=255)
               atcaccGGCATCGGGCGCGTGTCTGGCCGTGACTGCATGATCGTCTGCAACGATGCAACGGTGAAGGGCGGCACCTATTATCCGGTCACGGTGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGAGAACCGTCTGCCCTGCatttat                                                                                                                                       >  2:1166905/5‑150 (MQ=255)
                 caccGGCATCGGGCGCGTGTCTGGCCGTGACTGCATGATCGTCTGCAACGATGCAACGGTGAAGGGCGGCACCTATTATCCGGTCACGGTGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGAGAACCGTCTGCCCTGCatttatct                                                                                                                                     <  1:3569528/148‑1 (MQ=255)
                  accGGCATCGGGCGCGTGTCTGGCCGTGACTGCATGATCGTCTGCAACGATGCAACGGTGAAGGGCGGCACCTATTATCCGGTCACGGTGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGAGAACCGTCTGCCCTGCATTTATCtg                                                                                                                                    >  2:928522/2‑150 (MQ=255)
                   ccGGCATCGGCCGCGTGGAAGGCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCtgg                                                                                                                                   >  1:3371096/1‑150 (MQ=255)
                    cGGCATCGGCCGCGTGGAAGGCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTTCAGAACCGCCTGCCGTGCATCTATCtggt                                                                                                                                  >  2:4186479/1‑150 (MQ=255)
                    cGGCATCGGCCGCGTGGAAGGCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCtggt                                                                                                                                  <  2:3582957/150‑1 (MQ=255)
                     ggCATCGGGCGCGTGTCTGGCCGTGACTGCATGATCGTCTGCAACGATGCAACGGTGAAGGGCGGCACCTATTATCCGGTCACGGTGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGAGAACCGTCTGCCCTGCATTTATCtggtc                                                                                                                                 <  2:16559/150‑2 (MQ=255)
                     ggCATCGGGCGCGTGTCTGGCCGTGACTGCATGATCGTCTGCAACGATGCAACGGTGAAGGGCGGCACCTATTATCCGGTCACGGTGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGAGAACCGTCTGCCCTGCATTTATCtggtc                                                                                                                                 <  2:258614/150‑2 (MQ=255)
                     ggCATCGGGCGCGTGTCTGGCCGTGACTGCATGATCGTCTGCAACGATGCAACGGTGAAGGGCGGCACCTATTATCCGGTCACGGTGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGAGAACCGTCTGCCCTGCATTTATCtggtc                                                                                                                                 >  1:3717894/1‑149 (MQ=255)
                     ggCATCGGCCGCGTGGAAGGCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCtggtg                                                                                                                                 <  1:918278/150‑1 (MQ=255)
                     ggCATCGGCCGCGTGGAAGGCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCtggtg                                                                                                                                 <  2:4625405/150‑1 (MQ=255)
                     ggCATCGGCCGCGTGGAAGGCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCtggtg                                                                                                                                 <  2:4625471/150‑1 (MQ=255)
                           ggCCGCGTGGAAGGCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTcc                                                                                                                           >  1:2522935/1‑150 (MQ=255)
                              cgcgTGGAAGGCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCcggc                                                                                                                        <  1:809913/150‑1 (MQ=255)
                                  tGGAAGGCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGgcggcg                                                                                                                    >  2:269921/1‑150 (MQ=255)
                                   ggAAGGCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGgcgc                                                                                                                   <  2:370421/150‑1 (MQ=255)
                                       ggCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAAc                                                                                                               <  1:2160913/150‑1 (MQ=255)
                                       ggCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAAc                                                                                                               <  2:3267628/150‑1 (MQ=255)
                                          gTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTg                                                                                                            >  1:4982087/1‑150 (MQ=255)
                                            tgactGCATGATCGTCTGCAACGATGCAACGGTGAAGGGCGGCACCTATTATCCGGTCACGGTGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGAGAACCGTCTGCCCTGCATTTATCTGGTCGATTCCGGCGGCGCGAACCTGcc                                                                                                          <  2:2167146/146‑1 (MQ=255)
                                             gAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGccc                                                                                                         >  1:680699/1‑150 (MQ=255)
                                              aaTGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGcccc                                                                                                        <  1:4861324/150‑1 (MQ=255)
                                                  cATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCa                                                                                                    >  1:3857775/1‑150 (MQ=255)
                                                   aTGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAg                                                                                                   >  2:2984099/1‑150 (MQ=255)
                                                   aTGATCGTCTGCAACGATGCAACGGTGAAGGGCGGCACCCATTATCCGGTCACGGTGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGAGAACCGTCTGCCCTGCATTTATCTGGTCGATTCCGGCGGCGCGAACCTGcccaatcag                                                                                                   >  2:4352127/1‑144 (MQ=255)
                                                       tcgtctgcAACGATGCAACGGTGAAGGGCGGCACCTATTATCCGGTCACGGTGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGAGAACCGTCTGCCCTGCATTTATCTGGTCGATTCCGGCGGCGCGAACCTGCCCAATCAGGACg                                                                                               >  1:2908130/8‑150 (MQ=255)
                                                        cgtctgcAACGATGCAACGGTGAAGGGCGGCACCTATTATCCGGTCACGGTGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGAGAACCGTCTGCCCTGCATTTATCTGGTCGATTCCGGCGGCGCGAACCTGCCCAATCAGGACGa                                                                                              <  2:1321262/144‑1 (MQ=255)
                                                               aaCGATGCAACGGTGAAGGGCGGCACCTATTATCCGGTCACGGTGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGAGAACCGTCTGCCCTGCATTTATCTGGTCGATTCCGGCGGCGCGAACCTGCCCAATCAGGACGAGGttttt                                                                                       <  2:858398/150‑2 (MQ=255)
                                                               aaCGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTc                                                                                       <  1:4589583/150‑1 (MQ=255)
                                                                acgacgCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCACCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTcc                                                                                      <  2:3937442/150‑1 (MQ=255)
                                                                  gacgCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTcccc                                                                                    <  1:3152132/150‑1 (MQ=255)
                                                                  gacgCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTcccc                                                                                    <  1:595633/150‑1 (MQ=255)
                                                                    tgcaaCGGTGAAGGGCGGCACCTATTATCCGGTCACGGTGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGAGAACCGTCTGCCCTGCATTTATCTGGTCGATTCCGGCGGCGCGAACCTGCCCAATCAGGACGAGGTTTTTCCGGa                                                                                  <  1:201831/146‑1 (MQ=255)
                                                                    cgCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGa                                                                                  <  1:212928/150‑1 (MQ=255)
                                                                     gCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGAc                                                                                 >  2:1036860/1‑150 (MQ=255)
                                                                       cACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACcg                                                                               >  2:543045/1‑150 (MQ=255)
                                                                          ggTGAAGGGCGGCACCTATTATCCGGTCACGGTGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGAGAACCGTCTGCCCTGCATTTATCTGGTCGATTCCGGCGGCGCGAACCTGCCCAATCAGGACGAGGTTTTTCCGGATCGCGa                                                                            <  1:3831603/150‑1 (MQ=255)
                                                                          ggTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGa                                                                            <  1:4051909/150‑1 (MQ=255)
                                                                              aaaGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCcgg                                                                                                                         >  1:2779206/1‑101 (MQ=255)
                                                                              aaaGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCcgg                                                                                                                         <  2:2779206/101‑1 (MQ=255)
                                                                                 ggCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTc                                                                     <  1:3620683/150‑1 (MQ=255)
                                                                                   cGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCgg                                                                   <  2:40776/150‑1 (MQ=255)
                                                                                   cGGTTCGTACGACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCgg                                                                   <  2:2990143/150‑1 (MQ=255)
                                                                                     gTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGgc                                                                 <  2:2926794/150‑1 (MQ=255)
                                                                                     gTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGgc                                                                 >  1:22833/1‑150 (MQ=255)
                                                                                     gTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGgc                                                                 >  1:872456/1‑150 (MQ=255)
                                                                                     gTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGgc                                                                 >  1:3002916/1‑150 (MQ=255)
                                                                                          tactacCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGcc                                                                                                                                                 >  2:1430856/1‑65 (MQ=255)
                                                                                          tactacCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGcc                                                                                                                                                 <  1:1430856/65‑1 (MQ=255)
                                                                                          tactacCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCAtt                                                            >  2:3754523/1‑150 (MQ=255)
                                                                                             tacCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCa                                                                         <  2:4650224/134‑1 (MQ=255)
                                                                                             tacCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCa                                                                         >  1:4650224/1‑134 (MQ=255)
                                                                                             tacCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTttc                                                         >  1:2353004/1‑150 (MQ=255)
                                                                                               cccGCTGACGGTGAAGAAGCACCTTCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGTTGGACTCCGGCGGCGCCAACTTGCCACGCCAGGACTAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTttctt                                                       <  1:4338254/150‑1 (MQ=255)
                                                                                               cccGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTttctt                                                       <  1:3489728/150‑1 (MQ=255)
                                                                                               cccGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTttctt                                                       >  2:3108134/1‑150 (MQ=255)
                                                                                               cccGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTttctt                                                       <  2:3369002/150‑1 (MQ=255)
                                                                                                ccGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTttcttc                                                      >  1:2119786/1‑150 (MQ=255)
                                                                                                  ggtcaCGGTGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGCGAACCGTCTGCCCTGCATTTAGCTGGTCGATTCCGGCGGCGCGAACCTGCCCACTCAGGACGAGGGTTTTCCGGATCGCGATCCGTTCGGGCGCATCTTCTCCaa                                                    >  2:3065701/5‑150 (MQ=255)
                                                                                                   gtcaCGGTGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGAGAACCGTCTGCCCTGCATTTATCTGGTCGATTCCGGCGGCGCGAACCTGCCCAATCAGGACGAGGTTTTTCCGGATCGCGATCATTTCGGGCGCATCTTCTACaat                                                   <  1:98933/147‑2 (MQ=255)
                                                                                                    tGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAAcc                                                  >  1:1374612/1‑150 (MQ=255)
                                                                                                      aCGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAg                                                <  1:1951649/150‑1 (MQ=255)
                                                                                                        ggTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGc                                              <  2:2971476/150‑1 (MQ=255)
                                                                                                          tGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCa                                            >  1:3243435/1‑150 (MQ=255)
                                                                                                          tGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCa                                            <  2:4947051/150‑1 (MQ=255)
                                                                                                              gaagCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCCTTTCGGGCGCATTTTCTTCAACCAGGCCAACAt                                        >  1:4724939/1‑150 (MQ=255)
                                                                                                              gaagCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCaa                                           <  2:1908466/147‑1 (MQ=255)
                                                                                                              gaagCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACAt                                        <  2:3173867/150‑1 (MQ=255)
                                                                                                              gaagCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACAt                                        >  1:5086545/1‑150 (MQ=255)
                                                                                                                agCACCTGCGTTCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAATTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGa                                      <  1:1115123/150‑1 (MQ=255)
                                                                                                                  cACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAgc                                    <  1:4399071/150‑1 (MQ=255)
                                                                                                                   aCCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAgcg                                   >  2:3129924/1‑150 (MQ=255)
                                                                                                                        cGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCaa                              <  2:3371247/150‑1 (MQ=255)
                                                                                                                        cGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCaa                              <  2:4802031/150‑1 (MQ=255)
                                                                                                                           gCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCACGGc                           >  1:2987624/1‑150 (MQ=255)
                                                                                                                           gCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGc                           >  1:624993/1‑150 (MQ=255)
                                                                                                                             acaAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCAt                         >  2:866479/1‑150 (MQ=255)
                                                                                                                             acaAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCAt                         >  2:3683350/1‑150 (MQ=255)
                                                                                                                             acaAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCAt                         >  2:676954/1‑150 (MQ=255)
                                                                                                                               aaaCCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATcc                       >  1:3902976/1‑150 (MQ=255)
                                                                                                                                   cATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCa                   >  1:1691064/1‑150 (MQ=255)
                                                                                                                                   cATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCa                   >  2:4393591/1‑150 (MQ=255)
                                                                                                                                   cATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCa                   >  2:3688030/1‑150 (MQ=255)
                                                                                                                                       gCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATc               <  2:52403/150‑1 (MQ=255)
                                                                                                                                           tGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCg           >  2:1524625/1‑150 (MQ=255)
                                                                                                                                           tGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCg           >  2:3562758/1‑150 (MQ=255)
                                                                                                                                            gCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCgt          >  1:2751610/1‑150 (MQ=255)
                                                                                                                                              aGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCgtgg        <  1:1237762/150‑1 (MQ=255)
                                                                                                                                               gAACCGCCTGCCATGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCgtggt       <  1:4235937/150‑1 (MQ=255)
                                                                                                                                               gAACCGCCTGCCATGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCgtggt       <  1:3669283/150‑1 (MQ=255)
                                                                                                                                                 aCCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGa     <  1:1822354/150‑1 (MQ=255)
                                                                                                                                                  ccGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGAt    <  2:3509226/150‑1 (MQ=255)
                                                                                                                                                   cGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACCTGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATg   >  1:4533745/1‑150 (MQ=255)
                                                                                                                                                    gCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATgg  <  2:2422465/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCAGCGGCGGGCGTGATTGCCGGCATCGGCCGCGTGGAAGGCGTGGAATGCATGATCGTGGCCAACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGG  >  NC_002947/4591545‑4591842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑