Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,591,757 C→T 5.0% F157F (TTC→TTT)  mccB → methylcrotonyl‑CoA carboxylase biotin‑containing subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,591,7570CT 5.0% 570.6 / 11.6 179F157F (TTC→TTT) mccBmethylcrotonyl‑CoA carboxylase biotin‑containing subunit beta
Reads supporting (aligned to +/- strand):  ref base C (99/71);  new base T (4/5);  total (103/76)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

AACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCA  >  NC_002947/4591608‑4591906
                                                                                                                                                     |                                                                                                                                                     
aaCGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTc                                                                                                                                                       <  1:4589583/150‑1 (MQ=255)
aaCGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTc                                                                                                                                                       >  2:1623711/1‑150 (MQ=255)
   gacgCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTcccc                                                                                                                                                    <  1:595633/150‑1 (MQ=255)
   gacgCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTcccc                                                                                                                                                    <  1:3152132/150‑1 (MQ=255)
     cgCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGa                                                                                                                                                  <  1:213036/150‑1 (MQ=255)
          cGGTGAAGGGCGGCACCTATTATCCGGTCACGGTGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGAGAACCGTCTGCCCTGCATTTATCTGGTTGATTCCGGCGGCGCGAACCTGCCCAATCAGGACGAGGTTTTTCCGGATcgcg                                                                                                                                             <  2:1657951/150‑1 (MQ=255)
           ggTGAAGGGCGGCACCTATTATCCGGTCACGGTGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGAGAACCGTCTGCCCTGCATTTATCTGGTCGATTCCGGCGGCGCGAACCTGCCCAATCAGGACGAGGTTTTTCCGGATCGCGa                                                                                                                                            <  1:3831603/150‑1 (MQ=255)
           ggTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGa                                                                                                                                            <  1:4051909/150‑1 (MQ=255)
                  ggCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTc                                                                                                                                     <  1:3620683/150‑1 (MQ=255)
                    cGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCgg                                                                                                                                   <  2:40776/150‑1 (MQ=255)
                    cGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCgg                                                                                                                                   <  2:2990054/150‑1 (MQ=255)
                     ggTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCggg                                                                                                                                  >  2:2780863/1‑150 (MQ=255)
                      gTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGgc                                                                                                                                 >  1:22833/1‑150 (MQ=255)
                      gTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGgc                                                                                                                                 >  1:872426/1‑150 (MQ=255)
                      gTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGgc                                                                                                                                 <  2:2926794/150‑1 (MQ=255)
                      gTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGgc                                                                                                                                 >  2:121400/1‑150 (MQ=255)
                      gTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGgc                                                                                                                                 <  2:527189/150‑1 (MQ=255)
                           tactacCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCAtt                                                                                                                            >  2:3754523/1‑150 (MQ=255)
                              tacCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCa                                                                                                                                         >  1:4650224/1‑134 (MQ=255)
                              tacCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTttc                                                                                                                         >  1:2353004/1‑150 (MQ=255)
                              tacCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTttc                                                                                                                         >  1:1383363/1‑150 (MQ=255)
                                cccGCTGACGGTGAAGAAGCACCTTCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGTTGGACTCCGGCGGCGCCAACTTGCCACGCCAGGACTAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTttctt                                                                                                                       <  1:4338254/150‑1 (MQ=255)
                                cccGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTttctt                                                                                                                       <  1:3489728/150‑1 (MQ=255)
                                cccGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTttctt                                                                                                                       >  2:553543/1‑150 (MQ=255)
                                cccGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTttctt                                                                                                                       <  2:3369002/150‑1 (MQ=255)
                                cccGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTttctt                                                                                                                       <  1:917125/150‑1 (MQ=255)
                                 ccGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTttcttc                                                                                                                      >  1:2119786/1‑150 (MQ=255)
                                   ggtcaCGGTGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGCGAACCGTCTGCCCTGCATTTAGCTGGTCGATTCCGGCGGCGCGAACCTGCCCACTCAGGACGAGGGTTTTCCGGATCGCGATCCGTTCGGGCGCATCTTCTCCaa                                                                                                                    >  2:3065701/5‑150 (MQ=255)
                                       aCGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAg                                                                                                                <  1:1951649/150‑1 (MQ=255)
                                         ggTGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGAGAACCGTCTGCCCTGCATTTATCTGGTCGATTCCGGCGGCGCGAACCTGCCCAATCAGGACGAGGTTTTTCCGGATCGCGATCATTTCGGGCGCATCTTCTACAATCAGGc                                                                                                              >  2:43774/1‑150 (MQ=255)
                                         ggTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCCTTTCGGGCGCATTTTCTTCAACCAGGc                                                                                                              >  1:966313/1‑150 (MQ=255)
                                           tGAAGAAGCACCTGCGTGCGCAGGAAATCGCGGGCGAGAACCGTCTGCCCTGCATTTATCTGGTCGATTCCGGCGGCGCGAACCTGCCCAATCAGGACGAGGTTTTTCCGGATCGCGATCATTTCGGGCGCATCTTCTACAATCAGGcgc                                                                                                            >  2:3700006/1‑148 (MQ=255)
                                           tGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCa                                                                                                            >  1:3243435/1‑150 (MQ=255)
                                           tGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCa                                                                                                            <  2:4947051/150‑1 (MQ=255)
                                             aagaagCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAAc                                                                                                          <  2:4629442/150‑1 (MQ=255)
                                               gaagCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCaa                                                                                                           >  1:1908466/1‑147 (MQ=255)
                                               gaagCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCaa                                                                                                           <  2:1908466/147‑1 (MQ=255)
                                               gaagCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATGTTCTTCAACCAGGCCAACAt                                                                                                        >  1:4725506/1‑150 (MQ=255)
                                               gaagCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACGTGCCCCGCCAGGCCGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACag                                                                                                        >  1:520636/1‑149 (MQ=255)
                                                 agCACCTGCGTTCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAATTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGa                                                                                                      <  1:1115123/150‑1 (MQ=255)
                                                   cACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAgc                                                                                                    <  1:4399071/150‑1 (MQ=255)
                                                    aCCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAgcg                                                                                                   >  2:3129730/1‑150 (MQ=255)
                                                    aCCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAgcg                                                                                                   >  2:3129924/1‑150 (MQ=255)
                                                         cGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCaa                                                                                              <  2:2353004/150‑1 (MQ=255)
                                                         cGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCaa                                                                                              <  2:3371247/150‑1 (MQ=255)
                                                         cGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCaa                                                                                              <  2:4714743/150‑1 (MQ=255)
                                                            gCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCACGGc                                                                                           >  1:2987624/1‑150 (MQ=255)
                                                            gCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGc                                                                                           >  1:624993/1‑150 (MQ=255)
                                                              acaAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCAt                                                                                         >  2:3683350/1‑150 (MQ=255)
                                                              acaAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCAt                                                                                         >  2:3465643/1‑150 (MQ=255)
                                                              acaAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCAt                                                                                         >  2:866479/1‑150 (MQ=255)
                                                                aaaCCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATcc                                                                                       >  1:3902976/1‑150 (MQ=255)
                                                                aaaCCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATcc                                                                                       >  1:4458141/1‑150 (MQ=255)
                                                                aaaCCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATcc                                                                                       >  1:1093935/1‑150 (MQ=255)
                                                                    cATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCa                                                                                   >  2:3688016/1‑150 (MQ=255)
                                                                    cATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCa                                                                                   >  2:4393607/1‑150 (MQ=255)
                                                                      tCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGa                                                                                 >  1:990400/1‑150 (MQ=255)
                                                                        gCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATc                                                                               <  2:988662/150‑1 (MQ=255)
                                                                          ccTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAGGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGCGCGCGCAAGGCAGCCCGCAGATCGc                                                                             >  2:1698767/1‑150 (MQ=255)
                                                                            tGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCg                                                                           >  2:2471328/1‑150 (MQ=255)
                                                                            tGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCg                                                                           >  2:3562758/1‑150 (MQ=255)
                                                                             gCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCgt                                                                          >  1:2751610/1‑150 (MQ=255)
                                                                               aGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCgtgg                                                                        <  1:1237762/150‑1 (MQ=255)
                                                                                gAACCGCCTGCCATGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCgtggt                                                                       <  1:3669283/150‑1 (MQ=255)
                                                                                gAACCGCCTGCCATGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCgtggt                                                                       <  1:4236029/150‑1 (MQ=255)
                                                                                  aCCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGa                                                                     <  2:3951413/150‑1 (MQ=255)
                                                                                           cGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGt                                                            <  1:131835/150‑1 (MQ=255)
                                                                                             tGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGc                                                          <  1:1815365/150‑1 (MQ=255)
                                                                                              gCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCa                                                         <  2:5091205/150‑1 (MQ=255)
                                                                                               cATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGGATTTCGGGCGAATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTTCAc                                                        <  2:3189251/150‑1 (MQ=255)
                                                                                                atctatctGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCAcc                                                       >  1:3084462/1‑150 (MQ=255)
                                                                                                 tcgctctGGTGGACTCCGGCGGCGCCACCTGGCCCCGCCTGGACGACGTGGTCCCCGACCGCGAGCATTTCGGGCGCATGTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCAGCCCGCAGATCGCCGTGGTGAGGGGCTCGTGCACCg                                                      <  2:3371626/146‑1 (MQ=255)
                                                                                                  ctatctGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAATATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGc                                                     >  2:1307225/1‑150 (MQ=255)
                                                                                                     tctGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTgg                                                  >  2:4985172/1‑150 (MQ=255)
                                                                                                     tctGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTgg                                                  <  1:2780863/150‑1 (MQ=255)
                                                                                                       tggtggACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCCACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTggcg                                                >  2:2002923/1‑150 (MQ=255)
                                                                                                       tggtggACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTggcg                                                >  2:1435996/1‑150 (MQ=255)
                                                                                                            gACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGcgcg                                           >  1:1421724/1‑150 (MQ=255)
                                                                                                               tCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTAc                                        <  1:3615729/150‑1 (MQ=255)
                                                                                                                 cggcggCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGt                                      <  1:543045/150‑1 (MQ=255)
                                                                                                                   gcggcgCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGc                                    >  2:2486312/1‑150 (MQ=255)
                                                                                                                   gcggcgCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGc                                    <  2:2522935/150‑1 (MQ=255)
                                                                                                                   gcggcgCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGc                                    >  2:1081708/1‑150 (MQ=255)
                                                                                                                     ggcgCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCg                                  <  2:680551/150‑1 (MQ=255)
                                                                                                                       cgcCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGc                                <  1:2984099/150‑1 (MQ=255)
                                                                                                                           aaCTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATg                            >  1:4195459/1‑150 (MQ=255)
                                                                                                                           aaCTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATg                            <  2:3857775/150‑1 (MQ=255)
                                                                                                                           aaCTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATg                            >  1:723310/1‑150 (MQ=255)
                                                                                                                              ttGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGcc                         <  2:1373451/150‑1 (MQ=255)
                                                                                                                                 ccccGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCcgac                      >  2:3166432/1‑150 (MQ=255)
                                                                                                                                  cccGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTttc                                                                                                                         >  1:1684837/1‑50 (MQ=255)
                                                                                                                                  cccGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCcgacg                     >  2:4848208/1‑150 (MQ=255)
                                                                                                                                   ccGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCcgacga                    >  2:428091/1‑150 (MQ=255)
                                                                                                                                   ccGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCcgacga                    >  2:4246305/1‑150 (MQ=255)
                                                                                                                                    cGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGaa                   <  2:4978863/150‑1 (MQ=255)
                                                                                                                                         ggACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGAt              >  1:3975408/1‑150 (MQ=255)
                                                                                                                                         ggACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGAt              >  1:3807933/1‑150 (MQ=255)
                                                                                                                                         ggACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGAt              >  2:30973/1‑150 (MQ=255)
                                                                                                                                            cGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCAt           >  2:4524311/1‑150 (MQ=255)
                                                                                                                                                  gtTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTgcg     >  2:3766173/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACGACGCCACGGTAAAAGGCGGTTCGTACTACCCGCTGACGGTGAAGAAGCACCTGCGTGCACAAACCATCGCCCTGCAGAACCGCCTGCCGTGCATCTATCTGGTGGACTCCGGCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCA  >  NC_002947/4591608‑4591906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑