Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,099,211 G→C 7.0% intergenic (+232/+60) PP_mr66 → / ← acsA‑I P6/acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,099,2110GC 7.0% 230.2 / 4.9 78intergenic (+232/+60)PP_mr66/acsA‑IP6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (42/28);  new base C (4/2);  total (48/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGG  >  NC_002947/5099063‑5099359
                                                                                                                                                     |                                                                                                                                                    
cTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGc                                                                                                                                                     <  2:4315600/150‑1 (MQ=255)
cTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGc                                                                                                                                                     <  2:1554396/150‑1 (MQ=255)
cTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGc                                                                                                                                                     <  2:2059257/150‑1 (MQ=255)
  gACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCt                                                                                                                                                   <  2:4287039/150‑1 (MQ=255)
     tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGTCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                <  1:1741833/150‑1 (MQ=255)
     tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAATGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGccggga                                                                                                                                                >  2:55485/1‑146 (MQ=255)
     tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATTCGGGAGGTCAGGGGACAGCG‑TAGCCTGaa                                                                                                                                                >  1:3670081/1‑150 (MQ=255)
     tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGc                                                                                                                                                     >  2:1434487/1‑145 (MQ=255)
     tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGc                                                                                                                                                     <  1:1434487/145‑1 (MQ=255)
     tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                <  2:5815/150‑1 (MQ=255)
     tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                <  1:3004917/150‑1 (MQ=255)
     tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGCATGCGTGAGGTGAGGGGCCAGCT‑TTGccggta                                                                                                                                                >  1:1811391/1‑146 (MQ=255)
     gccGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                <  1:1044937/149‑1 (MQ=255)
         aTGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAATAGCGGGGGTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGTACTGa                                                                                                                                            >  2:3179121/1‑150 (MQ=255)
         aTGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGa                                                                                                                                            >  2:3179033/1‑150 (MQ=255)
          tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                           <  1:3661234/150‑1 (MQ=255)
          atcACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCACTCCGGGGCCGGTGACAGAACAGTAACCGATGATTCTCGCGTAACAATGCAAAACCGGGCTTGCCCGCGAATGCGAGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                           <  2:4601999/148‑1 (MQ=255)
                   aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                                  <  1:2152263/150‑1 (MQ=255)
                   aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                                  <  1:4468984/150‑1 (MQ=255)
                    cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                 <  1:1484398/150‑1 (MQ=255)
                    cGGGCCGGAATTGTGTCCTGGCGCCCCGCACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                 <  2:3290311/150‑1 (MQ=255)
                       gCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGGATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGGACGGACGCATTCGCgggga                                                                                                                              >  1:951641/1‑148 (MQ=255)
                        ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                                  <  1:4668791/145‑1 (MQ=255)
                        ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                                  >  2:4668791/1‑145 (MQ=255)
                          ggAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGc                                                                                                                           <  2:4632985/150‑1 (MQ=255)
                          ggAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGGGAGGTCAGGGGCCAGCT‑TAGCCTGAACGGACGCATTCGCGGGCAAGc                                                                                                                           >  2:2411816/1‑150 (MQ=255)
                          ggAATTGTTTCCTGGCGCCCCGAACCGGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGc                                                                                                                           <  2:4633198/150‑1 (MQ=255)
                                  ttCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCTTGCCCGcgaat                                                                                                                   <  2:2098642/150‑5 (MQ=255)
                                      tGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTGAGGGGGCAGCG‑TAGCCGGGACTGTCGCCGTCGCGGGCCAGCCCGCGCCTGCAg                                                                                                               >  1:947913/1‑150 (MQ=255)
                                        gcgcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGAAAGCGTGAGGTGAGGGGTCAGCG‑GGTGCTGAACGGTCGCCTTGGCGGGCCAGCCCGCGccaggcggg                                                                                                             >  1:2188816/1‑143 (MQ=255)
                                          gcCCCGCACAGGTTCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAggggg                                                                                                           >  2:1231821/1‑150 (MQ=39)
                                          gcCCCGCACAGGTTCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGAATGCGTGAGGTCCGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCGGCAggggg                                                                                                           >  2:1231822/1‑150 (MQ=255)
                                                    tgtgCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGGATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACAGACGCATTCGCGGGCAAGCCCCCTCCAGCAGGGGGTATTGCCggg                                                                                                 >  1:1672941/1‑150 (MQ=255)
                                                      gggggggCCGGTCAAACAACTTAACCCCATGTTTGTGGGTTAACATTGAAAAGGGGGGGTGGCCCGCGATTGGTGGCGGGCCGGGGACTCCT‑TAGCGTTAACTGACCATTTCGGGGCAAAGCCCGCTCCTGCAGGGGGTATTGCCGGGaa                                                                                               <  2:2644353/147‑1 (MQ=255)
                                                             ccGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGGATGCGGGTGGTGCGGGGGCAGCG‑TAGCCTGGACTGTCGCCTTCGCGGGCCAGCCCGCGCCCGCCGGGGGGGTTGCCGGGGGGGGGTTa                                                                                        >  1:4768419/1‑150 (MQ=255)
                                                                 tGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGCATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGGAGGGTTTACGcc                                                                                    >  2:1476189/1‑150 (MQ=255)
                                                                     aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCCAAATCGTGAGGTGAGGGGGCAGCT‑TATCCGGAAATGACGCATTAGCGGGCAAGCCCGCGCCAGCAGGGGGTATTGCCGGGGAGGGTTTAAGCCGAAg                                                                                >  1:4972232/1‑150 (MQ=255)
                                                                       aaCAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGcc                                                                              >  2:3348736/1‑150 (MQ=255)
                                                                            tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGc                                                                                                                           <  2:3010066/100‑1 (MQ=255)
                                                                            tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGc                                                                                                                           >  1:3010066/1‑100 (MQ=255)
                                                                                cGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                <  1:5116517/75‑1 (MQ=255)
                                                                                cGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                >  2:5116517/1‑75 (MQ=255)
                                                                                         tCGGGTAACAATGCAAAAGCGGGCTTGCCCCCCAAAGCGTGAGGTCAGGGGACAGCT‑TAGCCAGCACGGACCCATTCCCGGGCAAGCCCCCACCAGCAGGGGGTAGTGCCGGGAAGGGTTTACGCCCCAGCCCCGGTCATccccaagggg                                                            >  2:2913896/1‑144 (MQ=255)
                                                                                            ggTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCCGGTTCATCGCCTTGTGGGTa                                                         >  2:2012848/1‑150 (MQ=255)
                                                                                                       aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                 <  1:4781243/67‑1 (MQ=255)
                                                                                                       aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                 >  2:4781243/1‑67 (MQ=255)
                                                                                                          cgcgggcggGCCCGCGAAGGCGTGCGTTCTGGGGACCGCT‑TACCCTGAACTGCCGCATTCGGGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTg                                           <  2:3260008/149‑1 (MQ=255)
                                                                                                           gCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGt                                          >  2:2345217/1‑150 (MQ=255)
                                                                                                                 ttGCCCGCGAATGCGTGCGGTCTGGGGACAGCT‑TCGCCTGAACTGCGGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACcaca                                    <  2:2523510/150‑1 (MQ=255)
                                                                                                                     ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                                >  1:782352/1‑150 (MQ=255)
                                                                                                                     ccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCTCGCATTCGCGGGCAAGCCCGGTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                                >  2:4726017/1‑150 (MQ=21)
                                                                                                                      cgcgACTGGGTGCGGTCAGGGGACCGCT‑TAGCCCTACCTGACGCATTCGCGGGAAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg                               <  2:1649924/150‑1 (MQ=255)
                                                                                                                      cgcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg                               >  1:3239937/1‑150 (MQ=255)
                                                                                                                      cgcgAATGCGTCAGCTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCGGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGAt                                                    >  2:988047/1‑129 (MQ=18)
                                                                                                                      cgcgAATGCGTCAGCTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCGGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGAt                                                    <  1:988047/129‑1 (MQ=18)
                                                                                                                       gcgAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCCGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                              >  2:3934435/1‑150 (MQ=21)
                                                                                                                       gcgAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                              >  1:1123130/1‑150 (MQ=21)
                                                                                                                               gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTAc                                        >  2:1234456/1‑132 (MQ=255)
                                                                                                                               gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTAc                                        <  1:1234456/132‑1 (MQ=255)
                                                                                                                               gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                      >  1:308074/1‑150 (MQ=255)
                                                                                                                               gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                      >  1:5019730/1‑150 (MQ=255)
                                                                                                                               gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                      >  2:376503/1‑150 (MQ=255)
                                                                                                                               gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGG‑TTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAg                     >  1:4103387/1‑150 (MQ=255)
                                                                                                                                 gAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAgg                    >  2:2350516/1‑150 (MQ=255)
                                                                                                                                  aGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGt                   >  1:1622696/1‑150 (MQ=255)
                                                                                                                                     tctggggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga                <  2:2959883/147‑1 (MQ=255)
                                                                                                                                     ccacgggACAGCT‑CAGCCTCAACCGCCGCATTCGCGGGCAACCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGGGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga                <  2:1986405/146‑1 (MQ=255)
                                                                                                                                       aGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgaga              >  2:2014523/1‑150 (MQ=255)
                                                                                                                                         gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGata            >  1:3068620/1‑150 (MQ=255)
                                                                                                                                          ggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGatat           >  2:364174/1‑150 (MQ=255)
                                                                                                                                          ggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGatat           >  1:3621097/1‑150 (MQ=255)
                                                                                                                                           gACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGGATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATc          >  2:1192422/1‑150 (MQ=255)
                                                                                                                                              aGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGcc       >  1:1490672/1‑150 (MQ=255)
                                                                                                                                               gCT‑TAGCATGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATGGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGccc      >  2:2582512/1‑150 (MQ=255)
                                                                                                                                                cT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa     >  1:1150331/1‑150 (MQ=255)
                                                                                                                                                 t‑tAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAg    >  1:2070682/1‑150 (MQ=255)
                                                                                                                                                 t‑tAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAg    >  1:4644786/1‑150 (MQ=255)
                                                                                                                                                    aGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAggg  >  1:703226/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGG  >  NC_002947/5099063‑5099359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑