Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,099,217 A→C 8.3% intergenic (+238/+54) PP_mr66 → / ← acsA‑I P6/acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,099,2170AC 8.3% 190.7 / 6.1 68intergenic (+238/+54)PP_mr66/acsA‑IP6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (43/19);  new base C (4/2);  total (47/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCG  >  NC_002947/5099068‑5099364
                                                                                                                                                      |                                                                                                                                                   
tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGTCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                     <  1:1741833/150‑1 (MQ=255)
tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATTCGGGAGGTCAGGGGACAGCG‑TAGCCTGaa                                                                                                                                                     >  1:3670081/1‑150 (MQ=255)
tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                     <  1:3004917/150‑1 (MQ=255)
tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                     <  2:5815/150‑1 (MQ=255)
gccGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                     <  1:1044937/149‑1 (MQ=255)
    aTGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAATAGCGGGGGTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGTACTGa                                                                                                                                                 >  2:3179121/1‑150 (MQ=255)
    aTGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGa                                                                                                                                                 >  2:3179033/1‑150 (MQ=255)
     tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                                <  1:3661234/150‑1 (MQ=255)
     atcACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCACTCCGGGGCCGGTGACAGAACAGTAACCGATGATTCTCGCGTAACAATGCAAAACCGGGCTTGCCCGCGAATGCGAGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                                <  2:4601999/148‑1 (MQ=255)
              aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                                       <  1:4468984/150‑1 (MQ=255)
              aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                                       <  1:2152263/150‑1 (MQ=255)
               cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                      <  1:1484398/150‑1 (MQ=255)
               cGGGCCGGAATTGTGTCCTGGCGCCCCGCACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                      <  2:3290311/150‑1 (MQ=255)
                  gCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGGATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGGACGGACGCATTCGCgggga                                                                                                                                   >  1:951641/1‑148 (MQ=255)
                   ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                                       >  2:4668791/1‑145 (MQ=255)
                   ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                                       <  1:4668791/145‑1 (MQ=255)
                     ggAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGc                                                                                                                                <  2:4632985/150‑1 (MQ=255)
                     ggAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGGGAGGTCAGGGGCCAGCT‑TAGCCTGAACGGACGCATTCGCGGGCAAGc                                                                                                                                >  2:2411816/1‑150 (MQ=255)
                     ggAATTGTTTCCTGGCGCCCCGAACCGGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGc                                                                                                                                <  2:4633198/150‑1 (MQ=255)
                             ttCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCTTGCCCGcgaat                                                                                                                        <  2:2098642/150‑5 (MQ=255)
                                 tGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTGAGGGGGCAGCG‑TAGCCGGGACTGTCGCCGTCGCGGGCCAGCCCGCGCCTGCAg                                                                                                                    >  1:947913/1‑150 (MQ=255)
                                   gcgcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGAAAGCGTGAGGTGAGGGGTCAGCG‑GGTGCTGAACGGTCGCCTTGGCGGGCCAGCCCGCGccaggcggg                                                                                                                  >  1:2188816/1‑143 (MQ=255)
                                     gcCCCGCACAGGTTCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAggggg                                                                                                                >  2:1231821/1‑150 (MQ=39)
                                     gcCCCGCACAGGTTCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGAATGCGTGAGGTCCGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCGGCAggggg                                                                                                                >  2:1231822/1‑150 (MQ=255)
                                               tgtgCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGGATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACAGACGCATTCGCGGGCAAGCCCCCTCCAGCAGGGGGTATTGCCggg                                                                                                      >  1:1672941/1‑150 (MQ=255)
                                                 gggggggCCGGTCAAACAACTTAACCCCATGTTTGTGGGTTAACATTGAAAAGGGGGGGTGGCCCGCGATTGGTGGCGGGCCGGGGACTCCT‑TAGCGTTAACTGACCATTTCGGGGCAAAGCCCGCTCCTGCAGGGGGTATTGCCGGGaa                                                                                                    <  2:2644353/147‑1 (MQ=255)
                                                        ccGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGGATGCGGGTGGTGCGGGGGCAGCG‑TAGCCTGGACTGTCGCCTTCGCGGGCCAGCCCGCGCCCGCCGGGGGGGTTGCCGGGGGGGGGTTa                                                                                             >  1:4768419/1‑150 (MQ=255)
                                                            tGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGCATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGGAGGGTTTACGcc                                                                                         >  2:1476189/1‑150 (MQ=255)
                                                                aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCCAAATCGTGAGGTGAGGGGGCAGCT‑TATCCGGAAATGACGCATTAGCGGGCAAGCCCGCGCCAGCAGGGGGTATTGCCGGGGAGGGTTTAAGCCGAAg                                                                                     >  1:4972232/1‑150 (MQ=255)
                                                                  aaCAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGcc                                                                                   >  2:3348736/1‑150 (MQ=255)
                                                                       tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGc                                                                                                                                <  2:3010066/100‑1 (MQ=255)
                                                                       tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGc                                                                                                                                >  1:3010066/1‑100 (MQ=255)
                                                                           cGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                     <  1:5116517/75‑1 (MQ=255)
                                                                           cGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                     >  2:5116517/1‑75 (MQ=255)
                                                                                    tCGGGTAACAATGCAAAAGCGGGCTTGCCCCCCAAAGCGTGAGGTCAGGGGACAGCT‑TAGCCAGCACGGACCCATTCCCGGGCAAGCCCCCACCAGCAGGGGGTAGTGCCGGGAAGGGTTTACGCCCCAGCCCCGGTCATccccaagggg                                                                 >  2:2913896/1‑144 (MQ=255)
                                                                                       ggTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCCGGTTCATCGCCTTGTGGGTa                                                              >  2:2012848/1‑150 (MQ=255)
                                                                                                  aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                      <  1:4781243/67‑1 (MQ=255)
                                                                                                  aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                      >  2:4781243/1‑67 (MQ=255)
                                                                                                     cgcgggcggGCCCGCGAAGGCGTGCGTTCTGGGGACCGCT‑TACCCTGAACTGCCGCATTCGGGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTg                                                <  2:3260008/149‑1 (MQ=255)
                                                                                                      gCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGt                                               >  2:2345217/1‑150 (MQ=255)
                                                                                                            ttGCCCGCGAATGCGTGCGGTCTGGGGACAGCT‑TCGCCTGAACTGCGGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACcaca                                         <  2:2523510/150‑1 (MQ=255)
                                                                                                                ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                                     >  1:782352/1‑150 (MQ=255)
                                                                                                                ccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCTCGCATTCGCGGGCAAGCCCGGTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                                     >  2:4726017/1‑150 (MQ=21)
                                                                                                                 cgcgACTGGGTGCGGTCAGGGGACCGCT‑TAGCCCTACCTGACGCATTCGCGGGAAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg                                    <  2:1649924/150‑1 (MQ=255)
                                                                                                                 cgcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg                                    >  1:3239937/1‑150 (MQ=255)
                                                                                                                 cgcgAATGCGTCAGCTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCGGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGAt                                                         >  2:988047/1‑129 (MQ=18)
                                                                                                                 cgcgAATGCGTCAGCTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCGGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGAt                                                         <  1:988047/129‑1 (MQ=18)
                                                                                                                  gcgAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCCGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                                   >  2:3934435/1‑150 (MQ=21)
                                                                                                                  gcgAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                                   >  1:1123130/1‑150 (MQ=21)
                                                                                                                          gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTAc                                             >  2:1234456/1‑132 (MQ=255)
                                                                                                                          gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTAc                                             <  1:1234456/132‑1 (MQ=255)
                                                                                                                          gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                           >  1:5019730/1‑150 (MQ=255)
                                                                                                                          gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                           >  2:376503/1‑150 (MQ=255)
                                                                                                                          gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                           >  1:308074/1‑150 (MQ=255)
                                                                                                                          gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGG‑TTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAg                          >  1:4103387/1‑150 (MQ=255)
                                                                                                                            gAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAgg                         >  2:2350516/1‑150 (MQ=255)
                                                                                                                             aGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGt                        >  1:1622696/1‑150 (MQ=255)
                                                                                                                                tctggggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga                     <  2:2959883/147‑1 (MQ=255)
                                                                                                                                ccacgggACAGCT‑CAGCCTCAACCGCCGCATTCGCGGGCAACCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGGGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga                     <  2:1986405/146‑1 (MQ=255)
                                                                                                                                  aGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgaga                   >  2:2014523/1‑150 (MQ=255)
                                                                                                                                    gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGata                 >  1:3068620/1‑150 (MQ=255)
                                                                                                                                     ggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGatat                >  2:364174/1‑150 (MQ=255)
                                                                                                                                     ggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGatat                >  1:3621097/1‑150 (MQ=255)
                                                                                                                                      gACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGGATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATc               >  2:1192422/1‑150 (MQ=255)
                                                                                                                                         aGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGcc            >  1:1490672/1‑150 (MQ=255)
                                                                                                                                          gCT‑TAGCATGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATGGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGccc           >  2:2582512/1‑150 (MQ=255)
                                                                                                                                           cT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa          >  1:1150331/1‑150 (MQ=255)
                                                                                                                                            t‑tAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAg         >  1:4644786/1‑150 (MQ=255)
                                                                                                                                            t‑tAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAg         >  1:2070682/1‑150 (MQ=255)
                                                                                                                                               aGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAggg       >  1:703226/1‑150 (MQ=255)
                                                                                                                                                   ttaaCTGACGCTTGCGCGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGcgtc   <  2:934443/148‑1 (MQ=255)
                                                                                                                                                   tcaaCTGCCGCTGTCGCGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGcgtc   <  2:934444/148‑1 (MQ=255)
                                                                                                                                                   tGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGcgtc   >  2:2121485/1‑150 (MQ=255)
                                                                                                                                                   tGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGcgtc   <  2:1150331/150‑1 (MQ=255)
                                                                                                                                                   tGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGcgtc   >  1:1256695/1‑150 (MQ=255)
                                                                                                                                                   tGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGcgtc   >  1:1116817/1‑150 (MQ=255)
                                                                                                                                                    gAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGcgtcg  >  1:4790983/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
TCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCG  >  NC_002947/5099068‑5099364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑