Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,214,461 T→G 10.6% intergenic (+55/+39) PP_4593 → / ← PP_4594 hypothetical protein/cystathionine gamma‑synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,214,4610TG10.6% 367.2 / 31.1 132intergenic (+55/+39)PP_4593/PP_4594hypothetical protein/cystathionine gamma‑synthase
Reads supporting (aligned to +/- strand):  ref base T (52/66);  new base G (11/3);  total (63/69)
Fisher's exact test for biased strand distribution p-value = 2.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01

Only 100 of 137 total aligned reads displayed.

CGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATCCCCATCACCC  >  NC_002947/5214313‑5214609
                                                                                                                                                    |                                                                                                                                                    
 ggCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACcgtcg                                                                                                                                                    <  2:827257/150‑1 (MQ=255)
    aCCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAg                                                                                                                                                 <  1:1689826/150‑1 (MQ=255)
      cAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATATATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGa                                                                                                                                               >  1:133688/1‑150 (MQ=255)
            cgaAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGt                                                                                                                                         >  2:3686731/1‑150 (MQ=255)
             gaAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGtt                                                                                                                                        >  1:2409515/1‑150 (MQ=255)
              aaGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGttt                                                                                                                                       >  2:2055321/1‑150 (MQ=255)
              aaGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGttt                                                                                                                                       >  1:1057647/1‑150 (MQ=255)
               aGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTCCACCGTCGTAGGAGCGGGTTTa                                                                                                                                      <  2:2607717/150‑1 (MQ=255)
                  cGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTAccc                                                                                                                                   <  2:3430129/150‑1 (MQ=255)
                  cGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTAccc                                                                                                                                   <  1:4251702/150‑1 (MQ=255)
                  cGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTAccc                                                                                                                                   <  1:3810796/150‑1 (MQ=255)
                    tCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCcgc                                                                                                                                 <  1:686992/150‑1 (MQ=255)
                    tCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCcgc                                                                                                                                 <  1:1796201/150‑1 (MQ=255)
                      aaCCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACcgtcgt                                                                                                                                                   <  2:1308420/130‑1 (MQ=255)
                      aaCCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACcgtcgt                                                                                                                                                   >  1:1308420/1‑130 (MQ=255)
                       aCCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGaa                                                                                                                              >  2:2343536/1‑150 (MQ=255)
                          gCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAgg                                                                                                                           <  2:4512658/150‑1 (MQ=255)
                          gCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAgg                                                                                                                           >  1:393344/1‑150 (MQ=255)
                          gCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAgg                                                                                                                           >  2:3509475/1‑150 (MQ=255)
                                  aaGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCgg                                                                                                                   >  1:5016985/1‑150 (MQ=255)
                                   aGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGc                                                                                                                  <  1:1837147/150‑1 (MQ=255)
                                    ggAAAGGTTGCTGATCAAGGACCAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACCCCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGcc                                                                                                                 <  2:3404451/150‑1 (MQ=255)
                                     gAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGGTTACCCGCGAAAGGGCCGGCGGccc                                                                                                                >  1:1076049/1‑150 (MQ=255)
                                      aaaGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTTCGGCCCt                                                                                                               <  2:1861353/150‑1 (MQ=255)
                                      aaaGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCt                                                                                                               <  2:1221936/150‑1 (MQ=255)
                                      aaaGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCGTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCt                                                                                                               <  2:1861321/150‑1 (MQ=255)
                                        aGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGCAGGCCCttt                                                                                                             <  1:3286490/150‑3 (MQ=255)
                                         ggTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGCAGGCCCtttc                                                                                                            <  1:2364335/150‑4 (MQ=255)
                                            tGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTcacaca                                                                                                         <  2:360140/150‑1 (MQ=255)
                                             gCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAg                                                                                                        <  1:5027988/150‑1 (MQ=255)
                                             gCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGCAGGCCCtttcgcgg                                                                                                        <  2:213622/150‑8 (MQ=38)
                                                 aTCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGAc                                                                                                    >  2:3113238/1‑150 (MQ=255)
                                                  tCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAATCCCGCTCCTACACCGTCGTAGGAGCGGGTTTAACCGCGAAAGGGCCTGCGGCCCTCACACATCGAcc                                                                                                   >  1:296930/1‑150 (MQ=255)
                                                  tCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGAcc                                                                                                   >  1:4641542/1‑150 (MQ=255)
                                                  tCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCGCACACAGCGAcc                                                                                                   >  1:396911/1‑150 (MQ=255)
                                                   cAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCa                                                                                                  <  1:3193083/150‑1 (MQ=255)
                                                   cAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCa                                                                                                  <  1:5038187/150‑1 (MQ=255)
                                                    aaGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTGGCGGGGAAGCCCGCTCGTACACCGTGGTTGGAGCGGGTTTAACCGCGAAAGGGGCTGCGGGCCGCACACAGCGACCAg                                                                                                 >  1:1184/1‑150 (MQ=255)
                                                    aaGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGGTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAg                                                                                                 >  1:116781/1‑150 (MQ=255)
                                                       gACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACGGCGACCAgcgc                                                                                              >  1:1821521/1‑150 (MQ=255)
                                                        aCAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCt                                                                                             <  1:3587628/150‑1 (MQ=255)
                                                         cAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGACTCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCtt                                                                                            <  1:4636063/150‑1 (MQ=255)
                                                         cAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCtt                                                                                            <  2:3010852/150‑1 (MQ=255)
                                                            gATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCa                                                                                         >  1:2690838/1‑150 (MQ=255)
                                                              tCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAAc                                                                                       <  2:5037935/150‑1 (MQ=255)
                                                               cGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCACGCCCGCTCCTACACCGTCGGAGGAGCGGGGTTACCCGCGACAGGGCCGGCGGCCCTCACACAGCGACCCGCGCTGTCAACg                                                                                      >  2:2667603/1‑150 (MQ=255)
                                                                gAACGGATCATTCATCCGCCCAAATCCTGACAGGCATATATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGc                                                                                     <  2:133688/150‑1 (MQ=255)
                                                                 aaCGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGcc                                                                                    <  1:1952102/150‑1 (MQ=255)
                                                                 aaCGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTAGCGGGCAAGCCCCCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCCGCGCATTCAACGcc                                                                                    >  1:4815218/1‑150 (MQ=255)
                                                                    ggATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACGACGGTGTAGGAGCg                                                                                                                                            >  2:4187525/1‑91 (MQ=37)
                                                                     gATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCAt                                                                                <  1:2515825/150‑1 (MQ=255)
                                                                        cattcatCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCa                                                                             >  2:4442345/1‑150 (MQ=255)
                                                                        cattcatCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCa                                                                             >  2:1864744/1‑150 (MQ=255)
                                                                          ttcatCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAgg                                                                           <  1:2808458/150‑1 (MQ=255)
                                                                             atCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTcc                                                                        >  1:1565523/1‑150 (MQ=255)
                                                                                cGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCCGCGCATTCAAACCCCCAACCCGGTCCTCg                                                                     >  1:4330920/1‑150 (MQ=38)
                                                                                 gCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGa                                                                    <  2:48936/150‑1 (MQ=255)
                                                                                   ccAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGAcc                                                                  >  2:1566629/1‑150 (MQ=255)
                                                                                    cAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCa                                                                 <  1:1239351/150‑1 (MQ=255)
                                                                                           tGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACg                                                          <  2:2574305/150‑1 (MQ=255)
                                                                                            gACAGGCATCTATTGAATCCACCGGCCTCGTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACgg                                                         <  2:2976942/150‑1 (MQ=255)
                                                                                              cAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTa                                                       <  1:543395/150‑1 (MQ=255)
                                                                                                   aTCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACCGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCt                                                  >  2:1621453/1‑150 (MQ=255)
                                                                                                   aTCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCt                                                  >  2:3101497/1‑150 (MQ=255)
                                                                                                   aTCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCt                                                  <  1:4354797/150‑1 (MQ=255)
                                                                                                      tcttGATTCCTCCGGCCTCTTCGCGGGCAAGCCCTCTCCTACACCGTCGTAGGAGCGGGTTTACCCTCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACTGTAATCCTCGa                                               <  2:1241259/148‑1 (MQ=255)
                                                                                                      tATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGa                                               <  2:4053432/150‑1 (MQ=255)
                                                                                                        ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACgg                                                         <  1:735981/138‑1 (MQ=255)
                                                                                                        ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATc                                             >  2:636194/1‑150 (MQ=255)
                                                                                                        ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCCTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGCCCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATACTCGAta                                             >  1:2389956/1‑149 (MQ=255)
                                                                                                           aaTCCACCGGCCTCTTCGCGGGCAAGCCCCCTCCTAAACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCAGCGGCCCACACAAAGCGACCCGCGCTTTCAACGCCCCCTCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATcccg                                          >  1:4774503/1‑149 (MQ=38)
                                                                                                             tCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTAc                                        <  2:4765592/150‑1 (MQ=255)
                                                                                                               cACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCg                                      <  1:2055321/150‑1 (MQ=255)
                                                                                                                      cctttCGCGGGTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCg                               >  1:1220033/4‑150 (MQ=25)
                                                                                                                       ctttCGCGGGTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCgc                              >  1:4349411/3‑150 (MQ=37)
                                                                                                                         ttCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCgcag                            <  1:1506482/150‑1 (MQ=255)
                                                                                                                           cgcgGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTCTACCCGCGAAAGGGCCTGCGGCCCTCACACCGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTCCTCCTCGATCCCTACCGACAACCgcagcc                          >  2:2046131/1‑149 (MQ=255)
                                                                                                                            gcgGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGcagcag                         >  2:4637277/1‑150 (MQ=255)
                                                                                                                            gcgGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGcagcag                         >  2:797372/1‑150 (MQ=255)
                                                                                                                             cgGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAgg                        <  2:4641542/150‑1 (MQ=255)
                                                                                                                             cgGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAgg                        <  2:3157722/150‑1 (MQ=255)
                                                                                                                              gggCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGt                       >  2:1227412/1‑150 (MQ=255)
                                                                                                                               ggtaaacccGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGtt                      >  2:2427513/7‑150 (MQ=38)
                                                                                                                               ggtaaacccGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGtt                      >  2:2427257/7‑150 (MQ=38)
                                                                                                                                gtaaacccGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTtc                     >  1:4419367/6‑150 (MQ=38)
                                                                                                                                 taaacccGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTAcc                                       <  2:4055500/127‑1 (MQ=37)
                                                                                                                                 taaacccGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTAcc                                       >  1:4055500/5‑131 (MQ=37)
                                                                                                                                    gCCCGCTCCTACCCCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACCGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGc                 <  2:3169214/150‑1 (MQ=255)
                                                                                                                                    gCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGc                 <  1:2343536/150‑1 (MQ=255)
                                                                                                                                    gCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGc                 <  2:4499562/150‑1 (MQ=255)
                                                                                                                                     cccGCTCCTACGACGGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCt                >  1:2418309/1‑150 (MQ=255)
                                                                                                                                     cccGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCt                >  1:301960/1‑150 (MQ=255)
                                                                                                                                     cccGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCt                >  2:5002929/1‑150 (MQ=255)
                                                                                                                                           ccTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATccc          <  1:2950647/150‑1 (MQ=255)
                                                                                                                                            cTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATcccc         <  2:236496/150‑1 (MQ=255)
                                                                                                                                            cTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATcccc         <  2:1565523/150‑1 (MQ=255)
                                                                                                                                            cTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATcccc         >  1:4543149/1‑150 (MQ=255)
                                                                                                                                             tACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATCCCCa        <  1:3686731/150‑1 (MQ=255)
                                                                                                                                               cacCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCTGGTTCTCGCTGATCCCCATc      >  1:1376050/1‑150 (MQ=255)
                                                                                                                                                   gtcgtAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATCCCCATCAccc  >  2:589369/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATCCCCATCACCC  >  NC_002947/5214313‑5214609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑