BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,378,716	C→A	9.3%	L273L (CTG→CTT)	dnaK ←	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,378,716	0	C	A	9.3%	436.9 / 26.6	139	L273L (CTG→CTT)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand): ref base C (51/75); new base A (5/8); total (56/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 145 total aligned reads displayed.

GCGATGCGGCAAGGCTCGATGGTACGCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTC  >  NC_002947/5378567‑5378865

    tGCGGCAAGGCTCGATGGTACGCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCt                                                                                                                                                   <  1:4379832/150‑1 (MQ=255)

    tGCGGCAAGGCTCGATGGTACGCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCt                                                                                                                                                   <  1:2788518/150‑1 (MQ=255)

        gCAAGGCTCGATGGTACGCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGAGCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGAt                                                                                                                                               >  2:2989521/1‑150 (MQ=255)

        gCAAGGCTCGATGGTACGCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGAt                                                                                                                                               <  1:3438728/150‑1 (MQ=255)

          aaGGCTCGATGGTACGCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCt                                                                                                                                             <  1:3309190/150‑1 (MQ=255)

            ggCTCGATGGTACGCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTg                                                                                                                                           <  2:3196453/150‑1 (MQ=255)

            ggCTCGATGGTACGCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTg                                                                                                                                           <  2:910906/150‑1 (MQ=255)

            ggCTCGATGGTACGCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTg                                                                                                                                           <  2:683518/150‑1 (MQ=255)

            ggCTCGATGGTACGCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTg                                                                                                                                           <  1:202304/150‑1 (MQ=255)

               tCGATGGTACGCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCt                                                                                                                                        >  2:4460408/1‑150 (MQ=255)

                   tGGTACGCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCttttt                                                                                                                                    <  2:451705/150‑1 (MQ=255)

                     gTACGCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCt                                                                                                                                  <  1:2988608/150‑1 (MQ=255)

                      tACGCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTg                                                                                                                                 <  1:4188347/150‑1 (MQ=255)

                       aCGCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGc                                                                                                                                <  2:5102145/150‑1 (MQ=255)

                       aCGCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGc                                                                                                                                <  1:1823792/150‑1 (MQ=255)

                         gCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCgg                                                                                                                              <  1:856901/150‑1 (MQ=255)

                         gCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCgg                                                                                                                              <  1:3553032/150‑1 (MQ=255)

                         gCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCgg                                                                                                                              >  2:3580490/1‑150 (MQ=255)

                         gCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCgg                                                                                                                              >  1:3309788/1‑150 (MQ=255)

                            tGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGctt                                                                                                                           >  2:1295204/1‑150 (MQ=255)

                               ccAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCCGcttctt                                                                                                                        <  2:3390687/150‑1 (MQ=255)

                                ctggTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCttt                                                                                                                       <  1:2422717/148‑1 (MQ=255)

                                cAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCttt                                                                                                                       <  2:2087372/150‑1 (MQ=255)

                                cAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCttt                                                                                                                       <  2:4891890/150‑1 (MQ=255)

                                cAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCttt                                                                                                                       <  2:1586858/150‑1 (MQ=255)

                                 aGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTc                                                                                                                      <  2:23187/150‑1 (MQ=255)

                                 aGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTc                                                                                                                      <  2:292170/150‑1 (MQ=255)

                                      ttCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACg                                                                                                                 >  1:1359023/1‑150 (MQ=255)

                                      ttCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTACTGCGGCTTCTTTCAGACg                                                                                                                 >  2:1383281/1‑150 (MQ=255)

                                            cAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAg                                                                                                           >  1:899444/1‑150 (MQ=255)

                                             aGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAgg                                                                                                          <  2:4899941/150‑1 (MQ=255)

                                             aGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAgg                                                                                                          <  2:16659/150‑1 (MQ=255)

                                              gCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTGTCAGACGCTGCAggg                                                                                                         >  2:2064807/1‑150 (MQ=255)

                                               cGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGc                                                                                                        <  1:3431830/150‑1 (MQ=255)

                                                 aCTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCa                                                                                                      <  1:3963429/150‑1 (MQ=255)

                                                   tCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCaga                                                                                                    >  2:4097614/1‑150 (MQ=255)

                                                   tCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCaga                                                                                                    <  1:1929188/150‑1 (MQ=255)

                                                   tCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCaga                                                                                                    >  2:4757423/1‑150 (MQ=255)

                                                       gCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGAt                                                                                                >  2:1022115/1‑150 (MQ=255)

                                                        cTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCCGACGCTGCAGGGCCAGAGGATc                                                                                               <  1:3754789/150‑1 (MQ=255)

                                                        cTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATc                                                                                               <  2:483013/150‑1 (MQ=255)

                                                         ttGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCg                                                                                              <  1:4095211/150‑1 (MQ=255)

                                                         ttGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCg                                                                                              <  2:1853136/150‑1 (MQ=255)

                                                             cgcgGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGttct                                                                                          <  1:2089749/150‑1 (MQ=255)

                                                                gggAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCa                                                                                       <  2:320506/150‑1 (MQ=255)

                                                                gggAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCa                                                                                       <  2:469233/150‑1 (MQ=255)

                                                                   agaTCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGt                                                                                    <  1:4759110/150‑1 (MQ=255)

                                                                   agaTCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGt                                                                                    <  1:4759114/150‑1 (MQ=255)

                                                                       cTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCAt                                                                                >  1:4047398/1‑150 (MQ=255)

                                                                        ttCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATg                                                                               >  1:104775/1‑150 (MQ=255)

                                                                        ttCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATg                                                                               >  1:1137348/1‑150 (MQ=255)

                                                                        ttCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATg                                                                               >  2:1275829/1‑150 (MQ=255)

                                                                               tCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGAct                                                                        >  2:4695584/1‑150 (MQ=255)

                                                                                aaGGTGCTTAGGACCGGTCTGGTCAGCCGTGATGAATGGCAGGTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGccctgttc                                                                       <  1:3210599/149‑6 (MQ=255)

                                                                                 aGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGActct                                                                      >  1:3094274/1‑150 (MQ=255)

                                                                                  ggTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctt                                                                     >  2:275820/1‑150 (MQ=255)

                                                                                    tGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctttc                                                                   <  1:4052334/150‑1 (MQ=255)

                                                                                      cTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGAc                                                                                                                  <  2:954548/101‑1 (MQ=255)

                                                                                      cTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctttctt                                                                 <  2:4416774/150‑1 (MQ=255)

                                                                                       ttAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTg                                                                <  2:1545003/150‑1 (MQ=255)

                                                                                       ttAGGACCGGTCTGGTCAGCCGTGATGAATGGCAGGTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTg                                                                >  1:4864739/1‑150 (MQ=255)

                                                                                       ttAGGACCGGTCTGGTCAGCCGTGATGAATGGCAGGTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTg                                                                >  1:2932618/1‑150 (MQ=255)

                                                                                         aGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGaa                                                              <  2:1082973/150‑1 (MQ=255)

                                                                                         aGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGaa                                                              <  2:2710121/150‑1 (MQ=255)

                                                                                              cGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACtcgt                                                         >  2:3826259/1‑150 (MQ=255)

                                                                                              cGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACtcgt                                                         >  1:3152180/1‑150 (MQ=255)

                                                                                               ggTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACtcgtc                                                        <  2:2172394/150‑1 (MQ=255)

                                                                                               ggTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACtcgtc                                                        <  2:4047398/150‑1 (MQ=255)

                                                                                                   gCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACa                                                    >  1:1649052/1‑150 (MQ=255)

                                                                                                     aTCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAg                                                  >  1:1743234/1‑150 (MQ=255)

                                                                                                      tCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAAATCGTCGACAAgg                                                 <  2:23298/150‑1 (MQ=255)

                                                                                                        tGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTa                                               >  1:4936681/1‑150 (MQ=255)

                                                                                                        tGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGCACGACAGCTCGATCTTGGCATTTTCAGCGGCTTCTTACAGACGCTGCAGGGCCACAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTa                                               <  2:4305457/150‑1 (MQ=255)

                                                                                                         gCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAg                                              >  2:1887840/1‑150 (MQ=255)

                                                                                                         gCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAg                                              <  1:1029398/150‑1 (MQ=255)

                                                                                                            gTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCg                                           >  2:2617728/1‑150 (MQ=255)

                                                                                                               aTGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATc                                        <  2:2219493/150‑1 (MQ=255)

                                                                                                                tgaatggCAGGTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCa                                       <  1:519848/145‑1 (MQ=255)

                                                                                                                tGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctttc                                                                   <  2:4084881/122‑1 (MQ=255)

                                                                                                                   aCGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGgc                                    >  1:2391370/1‑150 (MQ=255)

                                                                                                                       cAGGTTGAGTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCAt                                <  2:3046139/150‑1 (MQ=255)

                                                                                                                       cAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCAt                                >  1:4874147/1‑150 (MQ=255)

                                                                                                                         ggTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGt                              >  2:4785686/1‑150 (MQ=255)

                                                                                                                          gTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTc                             >  1:4123038/1‑150 (MQ=255)

                                                                                                                               tttcggtctGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGt                        >  2:2387316/3‑150 (MQ=255)

                                                                                                                                gtcggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTc                       >  2:4310387/1‑150 (MQ=255)

                                                                                                                                  cggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCtt                     <  2:4321015/150‑1 (MQ=255)

                                                                                                                                   ggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTc                    <  1:617466/150‑1 (MQ=255)

                                                                                                                                   ggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTc                    <  1:1477439/150‑1 (MQ=255)

                                                                                                                                   ggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTc                    <  2:3017436/150‑1 (MQ=255)

                                                                                                                                       gACTGAGCGGACGACAGCTCGATCTTGGCTATTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCGGGCGCATGTCGAAGTCTTCGCCa                >  2:2369038/1‑150 (MQ=255)

                                                                                                                                        aCTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCAc               >  2:4768506/1‑150 (MQ=255)

                                                                                                                                           gAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGTCTTCACCGccga            <  1:1626153/150‑3 (MQ=255)

                                                                                                                                           gAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGTCTTCACCGccga            <  2:3635384/150‑3 (MQ=255)

                                                                                                                                               ggACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGaa        <  2:1516471/150‑1 (MQ=255)

                                                                                                                                               ggACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGaa        <  2:4663922/150‑1 (MQ=255)

                                                                                                                                                  cgacAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGgt     >  2:2272522/1‑150 (MQ=255)

                                                                                                                                                  cgacAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGgt     <  1:2735731/150‑1 (MQ=255)

                                                                                                                                                     cAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTc  <  1:3580490/150‑1 (MQ=255)

                                                                                                                                                     cAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTc  <  1:3602913/150‑1 (MQ=255)

GCGATGCGGCAAGGCTCGATGGTACGCTTGACCAGGTCTTCGACCAGCGACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTC  >  NC_002947/5378567‑5378865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`