BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,378,779	C→A	10.9%	L252L (CTG→CTT)	dnaK ←	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,378,779	0	C	A	10.9%	387.1 / 30.3	128	L252L (CTG→CTT)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand): ref base C (55/59); new base A (6/8); total (61/67)
Fisher's exact test for biased strand distribution p-value = 7.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 136 total aligned reads displayed.

GGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGA  >  NC_002947/5378631‑5378928

gggAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCa                                                                                                                                                      <  2:469233/150‑1 (MQ=255)

gggAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCa                                                                                                                                                      <  2:320506/150‑1 (MQ=255)

 ggAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTTCGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAg                                                                                                                                                     <  1:3884339/150‑1 (MQ=255)

   agaTCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGt                                                                                                                                                   <  1:4759110/150‑1 (MQ=255)

      tCTTCACGTTCAGGTGCTTAGGACCTGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGAATGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTcct                                                                                                                                                >  2:2111529/1‑149 (MQ=255)

       cTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCAt                                                                                                                                               >  1:4047398/1‑150 (MQ=255)

        ttCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATg                                                                                                                                              >  1:104775/1‑150 (MQ=255)

        ttCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATg                                                                                                                                              >  2:1275829/1‑150 (MQ=255)

        ttCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATg                                                                                                                                              >  1:1137348/1‑150 (MQ=255)

               tCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGAct                                                                                                                                       >  2:4695584/1‑150 (MQ=255)

                aaGGTGCTTAGGACCGGTCTGGTCAGCCGTGATGAATGGCAGGTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGccctgttc                                                                                                                                      <  1:3210599/149‑6 (MQ=255)

                 aGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGActct                                                                                                                                     >  1:3094274/1‑150 (MQ=255)

                  ggTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctt                                                                                                                                    >  2:275820/1‑150 (MQ=255)

                    tGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctttc                                                                                                                                  <  1:4052334/150‑1 (MQ=255)

                      cTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctttctt                                                                                                                                <  2:2226438/150‑1 (MQ=255)

                      cTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctttctt                                                                                                                                <  2:4416774/150‑1 (MQ=255)

                       ttAGGACCGGTCTGGTCAGCCGTGATGAATGGCAGGTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTg                                                                                                                               >  1:4864739/1‑150 (MQ=255)

                       ttAGGACCGGTCTGGTCAGCCGTGATGAATGGCAGGTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTg                                                                                                                               >  1:2932618/1‑150 (MQ=255)

                         aGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGaa                                                                                                                             <  2:2710121/150‑1 (MQ=255)

                         aGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGaa                                                                                                                             <  2:1082973/150‑1 (MQ=255)

                           gACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTACAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACt                                                                                                                           >  2:2368701/1‑150 (MQ=255)

                              cGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACtcgt                                                                                                                        >  2:3826259/1‑150 (MQ=255)

                               ggTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACtcgtc                                                                                                                       <  2:4047398/150‑1 (MQ=255)

                                   gCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACa                                                                                                                   >  1:1649052/1‑150 (MQ=255)

                                    cATCTGCAGTGATGTACGGCTGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCTGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACaa                                                                                                                  <  2:4443900/150‑1 (MQ=255)

                                     aTCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAg                                                                                                                 >  1:1743234/1‑150 (MQ=255)

                                      tCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAAATCGTCGACAAgg                                                                                                                <  2:23298/150‑1 (MQ=255)

                                        tGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTa                                                                                                              >  1:4936681/1‑150 (MQ=255)

                                        tGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGCACGACAGCTCGATCTTGGCATTTTCAGCGGCTTCTTACAGACGCTGCAGGGCCACAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTa                                                                                                              <  2:4305457/150‑1 (MQ=255)

                                         gCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAg                                                                                                             >  2:1887840/1‑150 (MQ=255)

                                         gCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAg                                                                                                             <  1:1029398/150‑1 (MQ=255)

                                           aGTGATGTACGGCTGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTc                                                                                                           <  2:3627209/150‑1 (MQ=255)

                                           aGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTc                                                                                                           >  1:1702433/1‑150 (MQ=255)

                                            gTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCg                                                                                                          >  2:2617728/1‑150 (MQ=255)

                                             tGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGa                                                                                                         >  1:360062/1‑150 (MQ=255)

                                               aTGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATc                                                                                                       <  2:2219493/150‑1 (MQ=255)

                                                tgaatggCAGGTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCa                                                                                                      <  1:519848/145‑1 (MQ=255)

                                                tGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctttc                                                                                                                                  >  1:4084881/1‑122 (MQ=255)

                                                  tACGGCAGGTTGACGTCGGTCGACTGCGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAgg                                                                                                    <  2:4314436/150‑1 (MQ=255)

                                                   atggCAGGTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAgac                                                                                                   <  2:2458940/148‑3 (MQ=255)

                                                       cAGGTTGAGTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCAt                                                                                               <  2:3046139/150‑1 (MQ=255)

                                                       cAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCAt                                                                                               >  1:4874147/1‑150 (MQ=255)

                                                         ggTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGt                                                                                             >  2:4785682/1‑150 (MQ=255)

                                                          gTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTc                                                                                            >  1:4123038/1‑150 (MQ=255)

                                                          gTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTc                                                                                            >  1:1249512/1‑150 (MQ=255)

                                                             gACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGaa                                                                                         <  1:416551/150‑1 (MQ=255)

                                                               tttcggtctGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGt                                                                                       >  2:2387316/3‑150 (MQ=255)

                                                                gtcggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTc                                                                                      >  2:4310387/1‑150 (MQ=255)

                                                                  cggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCtt                                                                                    <  2:4321015/150‑1 (MQ=255)

                                                                   ggtctgcTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGTCTTc                                                                                   >  2:3859707/7‑150 (MQ=255)

                                                                   ggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTc                                                                                   <  1:185014/150‑1 (MQ=255)

                                                                   ggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTc                                                                                   <  2:3017436/150‑1 (MQ=255)

                                                                   ggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTc                                                                                   <  1:617466/150‑1 (MQ=255)

                                                                   ggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTc                                                                                   <  2:1359023/150‑1 (MQ=255)

                                                                     tcgACTGAGCGGTCGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGTACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGc                                                                                 <  1:2855088/150‑1 (MQ=255)

                                                                       gACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCa                                                                               >  2:4972999/1‑150 (MQ=255)

                                                                        gcTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGTCTTCAccgc                                                                              <  1:4859876/149‑3 (MQ=255)

                                                                        aCTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCAc                                                                              >  2:4768506/1‑150 (MQ=255)

                                                                           gAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGTCTTCACCGccga                                                                           <  2:3635384/150‑3 (MQ=255)

                                                                           gAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGTCTTCACCGccga                                                                           <  1:1626153/150‑3 (MQ=255)

                                                                               ggACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGaa                                                                       <  2:4663922/150‑1 (MQ=255)

                                                                               ggACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGaa                                                                       <  2:1516471/150‑1 (MQ=255)

                                                                                gacgacAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAg                                                                      >  1:3157418/1‑150 (MQ=255)

                                                                                  cgacAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGgt                                                                    >  2:2272522/1‑150 (MQ=255)

                                                                                  cgacAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGgt                                                                    <  1:2735731/150‑1 (MQ=255)

                                                                                   gaaaGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGTCTTCACCGCCGAGGAACgtg                                                                   >  1:4674877/4‑150 (MQ=255)

                                                                                     cAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTc                                                                 <  1:3602913/150‑1 (MQ=255)

                                                                                               ttGGCTTTTTCTGCTGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTa                                                       <  1:1383281/150‑1 (MQ=255)

                                                                                               ttGGCATTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTa                                                       <  1:2989521/150‑1 (MQ=255)

                                                                                                    tttttCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAg                                                  >  1:56131/1‑150 (MQ=255)

                                                                                                    tttttCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGACGATCAGGAGCATGTCGAAGTCTTCGAAACACATGAAGGAGTCGCCGTTGGTAGCCAg                                                  >  1:963647/1‑150 (MQ=255)

                                                                                                          tGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTc                                            <  1:4117285/150‑1 (MQ=255)

                                                                                                            cGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctttctt                                                                                                                                <  1:2262960/64‑1 (MQ=255)

                                                                                                            cGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctttctt                                                                                                                                <  1:2263158/64‑1 (MQ=255)

                                                                                                            cGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctttctt                                                                                                                                >  2:2262960/1‑64 (MQ=255)

                                                                                                             ggCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGaa                                         <  1:1059373/150‑1 (MQ=255)

                                                                                                              gCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAAc                                        >  1:3357962/1‑150 (MQ=255)

                                                                                                               cttcttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACt                                       >  1:3150377/1‑150 (MQ=255)

                                                                                                                ttcttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTg                                      >  2:1004530/1‑150 (MQ=255)

                                                                                                                 tcttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTgg                                     >  2:1069482/1‑150 (MQ=255)

                                                                                                                 tcttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTAGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTgg                                     <  1:4355325/150‑1 (MQ=255)

                                                                                                                  cttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGt                                    >  2:4476543/1‑150 (MQ=255)

                                                                                                                   tttCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTa                                                >  2:367927/1‑137 (MQ=255)

                                                                                                                   tttCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTa                                                <  1:367927/137‑1 (MQ=255)

                                                                                                                   tttCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTg                                   <  1:4460408/150‑1 (MQ=255)

                                                                                                                     tCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCt                                 >  1:2732403/1‑150 (MQ=255)

                                                                                                                              gCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCAACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGa                        >  1:4514273/1‑150 (MQ=255)

                                                                                                                                 gggCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACtt                     >  1:953223/1‑150 (MQ=255)

                                                                                                                                    ccAGAGGAGCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCgg                  >  2:4823110/1‑150 (MQ=255)

                                                                                                                                     cAGAGGATCGTACTTCAGGACCATGCCCGACTCTTTCTTGAACACGTCGACACGGTAGTCGATCAGGCGCATGACGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGc                 <  1:1967887/150‑1 (MQ=255)

                                                                                                                                      agagGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCg                >  2:4371694/1‑150 (MQ=255)

                                                                                                                                        agGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGAt              <  2:1137348/150‑1 (MQ=255)

                                                                                                                                            tCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCg          >  1:421613/1‑150 (MQ=255)

                                                                                                                                            tCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCg          <  1:2292565/150‑1 (MQ=255)

                                                                                                                                              gTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGAt        >  1:1631777/1‑150 (MQ=255)

                                                                                                                                                 cttcAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGAc     >  1:4978824/1‑150 (MQ=255)

                                                                                                                                                   tcAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCg   >  2:4682648/1‑150 (MQ=255)

                                                                                                                                                   tcAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCg   >  1:1806992/1‑150 (MQ=255)

                                                                                                                                                    cAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGa  >  1:2850226/1‑150 (MQ=255)

                                                                                                                                                    cAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGa  >  1:4370899/1‑150 (MQ=255)

GGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGA  >  NC_002947/5378631‑5378928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`