BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,378,821	G→T	8.0%	D238E (GAC→GAA)	dnaK ←	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,378,821	0	G	T	8.0%	405.9 / 19.2	125	D238E (GAC→GAA)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand): ref base G (64/51); new base T (4/6); total (68/57)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 131 total aligned reads displayed.

GCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAACGATGACGGT  >  NC_002947/5378672‑5378970

gCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAg                                                                                                                                                       <  1:1029398/150‑1 (MQ=255)

gCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAg                                                                                                                                                       >  2:1887840/1‑150 (MQ=255)

  aGTGATGTACGGCTGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTc                                                                                                                                                     <  2:3627209/150‑1 (MQ=255)

  aGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTc                                                                                                                                                     >  1:1702433/1‑150 (MQ=255)

   gTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCg                                                                                                                                                    >  2:2617728/1‑150 (MQ=255)

    tGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGa                                                                                                                                                   >  1:360062/1‑150 (MQ=255)

       tgaatggCAGGTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCa                                                                                                                                                <  1:519848/145‑1 (MQ=255)

         tACGGCAGGTTGACGTCGGTCGACTGCGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAgg                                                                                                                                              <  2:4314436/150‑1 (MQ=255)

          atggCAGGTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAgac                                                                                                                                             <  2:2458940/148‑3 (MQ=255)

          aCGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGgc                                                                                                                                             >  1:2391370/1‑150 (MQ=255)

              cAGGTTGAGTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCAt                                                                                                                                         <  2:3046139/150‑1 (MQ=255)

              cAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCAt                                                                                                                                         >  1:4874147/1‑150 (MQ=255)

                ggTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGt                                                                                                                                       >  2:4785686/1‑150 (MQ=255)

                ggTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGt                                                                                                                                       >  2:4785682/1‑150 (MQ=255)

                 gTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTc                                                                                                                                      >  1:4123038/1‑150 (MQ=255)

                 gTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTc                                                                                                                                      >  1:1249512/1‑150 (MQ=255)

                    gACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGaa                                                                                                                                   <  1:416551/150‑1 (MQ=255)

                      tttcggtctGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGt                                                                                                                                 >  2:2387316/3‑150 (MQ=255)

                       gtcggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTc                                                                                                                                >  2:4310387/1‑150 (MQ=255)

                         cggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCtt                                                                                                                              <  2:4321015/150‑1 (MQ=255)

                          ggtctgcTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGTCTTc                                                                                                                             >  2:3859707/7‑150 (MQ=255)

                          ggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTc                                                                                                                             <  2:1359023/150‑1 (MQ=255)

                          ggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTc                                                                                                                             <  1:617466/150‑1 (MQ=255)

                          ggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTc                                                                                                                             <  1:1477439/150‑1 (MQ=255)

                          ggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTc                                                                                                                             <  2:3017436/150‑1 (MQ=255)

                           gtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCg                                                                                                                            <  1:4916886/150‑1 (MQ=255)

                            tcgACTGAGCGGTCGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGTACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGc                                                                                                                           <  1:2855088/150‑1 (MQ=255)

                              gACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCa                                                                                                                         >  2:4972999/1‑150 (MQ=255)

                              gACTGAGCGGACGACAGCTCGATCTTGGCTATTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCGGGCGCATGTCGAAGTCTTCGCCa                                                                                                                         >  2:2369038/1‑150 (MQ=255)

                               aCTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCAc                                                                                                                        >  2:4768506/1‑150 (MQ=255)

                                  gAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGTCTTCACCGccga                                                                                                                     <  1:1626153/150‑3 (MQ=255)

                                  gAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGTCTTCACCGccga                                                                                                                     <  2:3635384/150‑3 (MQ=255)

                                      ggACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGaa                                                                                                                 <  2:4663922/150‑1 (MQ=255)

                                      ggACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGaa                                                                                                                 <  2:1516471/150‑1 (MQ=255)

                                       gacgacAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAg                                                                                                                >  1:3157418/1‑150 (MQ=255)

                                         cgacAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGgt                                                                                                              >  2:2272522/1‑150 (MQ=255)

                                         cgacAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGgt                                                                                                              <  1:2735731/150‑1 (MQ=255)

                                          gaaaGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGTCTTCACCGCCGAGGAACgtg                                                                                                             >  1:4674877/4‑150 (MQ=255)

                                            cAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTc                                                                                                           <  1:3602913/150‑1 (MQ=255)

                                            cAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTc                                                                                                           <  1:3580490/150‑1 (MQ=255)

                                               cTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACCAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGcc                                                                                                        >  1:38534/1‑150 (MQ=255)

                                                   aTCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTg                                                                                                    <  1:1072150/150‑1 (MQ=255)

                                                           tttttCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAg                                                                                            >  1:56131/1‑150 (MQ=255)

                                                           tttttCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGACGATCAGGAGCATGTCGAAGTCTTCGAAACACATGAAGGAGTCGCCGTTGGTAGCCAg                                                                                            >  1:963647/1‑150 (MQ=255)

                                                                 tGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTc                                                                                      <  1:4117285/150‑1 (MQ=255)

                                                                 tGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTc                                                                                      <  1:3094222/150‑1 (MQ=255)

                                                                    ggCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGaa                                                                                   <  1:1059373/150‑1 (MQ=255)

                                                                      cttcttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACt                                                                                 >  1:3150377/1‑150 (MQ=255)

                                                                       ttcttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTg                                                                                >  2:1004530/1‑150 (MQ=255)

                                                                       ttcttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTg                                                                                <  1:2064807/150‑1 (MQ=255)

                                                                        tcttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTgg                                                                               >  2:1069482/1‑150 (MQ=255)

                                                                        tcttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTAGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTgg                                                                               <  1:4355325/150‑1 (MQ=255)

                                                                         cttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGt                                                                              >  2:4476543/1‑150 (MQ=255)

                                                                          tttCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTa                                                                                          >  2:367927/1‑137 (MQ=255)

                                                                           ttCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACTAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGc                                                                            <  2:4509941/150‑1 (MQ=255)

                                                                               gACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCAc                                                                        >  2:4318779/1‑150 (MQ=255)

                                                                                aCGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCAcc                                                                       >  2:2983676/1‑150 (MQ=255)

                                                                                   cTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTc                                                                    <  1:4494529/150‑1 (MQ=255)

                                                                                     gCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCAACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGa                                                                  >  1:4514273/1‑150 (MQ=255)

                                                                                        gggCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACtt                                                               >  1:953223/1‑150 (MQ=255)

                                                                                        gggCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACtt                                                               >  1:5024946/1‑150 (MQ=255)

                                                                                            cAGAGGATCGTACTTCAGGACCATGCCCGACTCTTTCTTGAACACGTCGACACGGTAGTCGATCAGGCGCATGACGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGc                                                           <  1:1967887/150‑1 (MQ=255)

                                                                                             agagGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCg                                                          >  2:4371694/1‑150 (MQ=255)

                                                                                             agagGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCg                                                          >  1:4336907/1‑150 (MQ=255)

                                                                                               agGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGAt                                                        <  2:1137348/150‑1 (MQ=255)

                                                                                                   tCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCg                                                    >  1:421613/1‑150 (MQ=255)

                                                                                                     gTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGAt                                                  >  1:1631777/1‑150 (MQ=255)

                                                                                                          tcAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCg                                             >  1:1806992/1‑150 (MQ=255)

                                                                                                          tcAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCg                                             >  1:1807388/1‑150 (MQ=255)

                                                                                                          tcAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCg                                             >  1:406402/1‑150 (MQ=255)

                                                                                                           cAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGa                                            >  1:2850226/1‑150 (MQ=255)

                                                                                                           cAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGa                                            >  1:4370899/1‑150 (MQ=255)

                                                                                                              gTCCAGGCCCGACTCTTTCTTGAACTCGTCGACCAGGTAGTCGATCAGGCGCAGGTCGAAGTCTTCGCCTCCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGAGGACCGAAAc                                         <  1:2369038/150‑1 (MQ=255)

                                                                                                                   tGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGa                                    <  1:1022115/150‑1 (MQ=255)

                                                                                                                    gCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGaa                                   >  2:4881515/1‑150 (MQ=255)

                                                                                                                    gCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGaa                                   <  1:2368701/150‑1 (MQ=255)

                                                                                                                    gCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGaa                                   <  1:2111529/150‑1 (MQ=255)

                                                                                                                    gCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGaa                                   <  2:3309788/150‑1 (MQ=255)

                                                                                                                    gCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGaa                                   <  2:3309792/150‑1 (MQ=255)

                                                                                                                       cGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGt                                >  1:1233351/1‑150 (MQ=255)

                                                                                                                        gACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTa                               <  1:1275829/150‑1 (MQ=255)

                                                                                                                            ctttcttGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACTGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTaccac                           <  1:275820/150‑1 (MQ=255)

                                                                                                                             tttcttGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTaccacc                          >  1:4056257/1‑150 (MQ=255)

                                                                                                                               tcttGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTaccaccac                        >  1:4183130/1‑150 (MQ=255)

                                                                                                                                 ttGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCAccc                      >  1:3002031/1‑150 (MQ=255)

                                                                                                                                     aCTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGt                  >  2:3881375/1‑150 (MQ=255)

                                                                                                                                      cTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTc                 <  1:4757423/150‑1 (MQ=255)

                                                                                                                                      cTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTc                 <  1:4097614/150‑1 (MQ=255)

                                                                                                                                       tcgtcgACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCa                >  2:545885/1‑150 (MQ=255)

                                                                                                                                       tcgtcgACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCa                >  2:2624731/1‑150 (MQ=255)

                                                                                                                                       tcgtcgACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCa                >  1:4743596/1‑150 (MQ=255)

                                                                                                                                          tcgACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATaa             >  1:4338167/1‑150 (MQ=255)

                                                                                                                                          tcgACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATaa             >  1:2908950/1‑150 (MQ=255)

                                                                                                                                              cAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAACGa         >  2:2503654/1‑150 (MQ=255)

                                                                                                                                               aaGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAACGAt        <  1:1887840/150‑1 (MQ=255)

                                                                                                                                                    aGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAACGATGACgg   >  1:2973923/1‑150 (MQ=255)

                                                                                                                                                     gTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAACGATGACGgt  <  2:3152180/150‑1 (MQ=255)

                                                                                                                                                     gTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAACGATGACGgt  <  2:1649052/150‑1 (MQ=255)

                                                                                                                                                     gTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAACGATGACGgt  <  1:4972999/150‑1 (MQ=255)

                                                                                                                                                     gTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAACGATGACGgt  <  1:4476543/150‑1 (MQ=255)

GCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAACGATGACGGT  >  NC_002947/5378672‑5378970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`