BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,420,788	G→T	11.1%	P85T (CCT→ACT)	PP_4758 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,420,788	0	G	T	11.1%	486.4 / 37.2	160	P85T (CCT→ACT)	PP_4758	MFS transporter
Reads supporting (aligned to +/- strand): ref base G (85/57); new base T (5/13); total (90/70)
Fisher's exact test for biased strand distribution p-value = 1.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

TGCTTCGGCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGA  >  NC_002947/5420639‑5420934

      ggCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTc                                                                                                                                               >  1:3177732/1‑150 (MQ=255)

      ggCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTc                                                                                                                                               <  2:4281404/150‑1 (MQ=255)

       gCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCg                                                                                                                                              >  2:3183286/1‑150 (MQ=255)

         cACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGaa                                                                                                                                            <  2:912767/150‑1 (MQ=255)

         cACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGaa                                                                                                                                            <  1:4205008/150‑1 (MQ=255)

          aCCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTACCTTCGAAc                                                                                                                                           <  2:2872072/150‑1 (MQ=255)

          aCCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTACCTTCGAAc                                                                                                                                           <  2:586521/150‑1 (MQ=255)

           ccccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTACCTTCGAACa                                                                                                                                          <  1:1816761/150‑1 (MQ=255)

           ccccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTACCTTCGAACa                                                                                                                                          <  1:1816346/150‑1 (MQ=255)

           ccccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTACCTTCGAACa                                                                                                                                          <  2:4923519/150‑1 (MQ=255)

           ccccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTACCTTCGAACa                                                                                                                                          <  2:4923506/150‑1 (MQ=255)

            cccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTAc                                                                                                                                                  <  2:5085666/141‑1 (MQ=255)

            cccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑g                                                                                                                                        >  2:3276404/1‑150 (MQ=255)

             ccGAGCAGAATACTCAGCACGTATAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCATGTGAACATTATACTGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑gc                                                                                                                                       <  2:25208/150‑1 (MQ=255)

             ccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTACCTTCGAACatg                                                                                                                                        <  1:4647635/150‑3 (MQ=255)

             ccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑gc                                                                                                                                       <  1:4930747/150‑1 (MQ=255)

             ccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑gc                                                                                                                                       <  1:3604890/150‑1 (MQ=255)

               gAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑gcgc                                                                                                                                     <  1:2369368/150‑1 (MQ=255)

                   aGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTaa                                                                                                                                 >  2:16004/1‑150 (MQ=255)

                    gAAAACGCAGCACGTATAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCACCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTAGAAGGTACTTCGAACA‑GCGCGTAAc                                                                                                                                >  1:2952550/1‑150 (MQ=255)

                    gAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAAc                                                                                                                                >  1:4066303/1‑150 (MQ=255)

                     aaaaCGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAAcc                                                                                                                               >  2:2028561/1‑150 (MQ=255)

                     aaaaCGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAAcc                                                                                                                               >  2:4603742/1‑150 (MQ=255)

                     aaaaCGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAAcc                                                                                                                               >  2:31620/1‑150 (MQ=255)

                       aaCGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGTCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGa                                                                                                                             <  2:1783557/150‑1 (MQ=255)

                         cGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATg                                                                                                                           <  1:461139/150‑1 (MQ=255)

                          gcagcaCGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATga                                                                                                                          >  2:4752820/1‑150 (MQ=255)

                          gcagcaCGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATga                                                                                                                          <  1:2165290/150‑1 (MQ=255)

                           cagcaCGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATgaa                                                                                                                         <  1:2927406/150‑1 (MQ=255)

                              caCGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATgaagaa                                                                                                                      <  1:3506440/150‑1 (MQ=255)

                              caCGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATgaagaa                                                                                                                      >  1:150070/1‑150 (MQ=255)

                                cGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACa                                                                                                                    <  1:4772078/150‑1 (MQ=255)

                                cGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACa                                                                                                                    <  1:1348031/150‑1 (MQ=255)

                                  tAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCAACATCCCACAGGTGTACATGATACGGGTCAGCCACATCCGTGCACCGAGCTTTTGCAGCAGCATGTTCGAAGTTAATTCGAACA‑TCGCGTAACCGATGAAGAACAGc                                                                                                                  <  2:3239208/150‑1 (MQ=255)

                                     aaCTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCg                                                                                                               >  1:324905/1‑150 (MQ=255)

                                          ggTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCAcc                                                                                                          >  2:2387340/1‑150 (MQ=255)

                                          ggTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCAcc                                                                                                          >  1:2280972/1‑150 (MQ=255)

                                          ggTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCAcc                                                                                                          >  1:1665590/1‑150 (MQ=255)

                                            tttCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGa                                                                                                        >  1:250116/1‑150 (MQ=255)

                                            tttCGTTCTGCACAAAGGCCATGGCCGTGCCCACCAGCCACCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCAGGCACCGa                                                                                                        >  2:2269219/1‑150 (MQ=255)

                                               cGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGc                                                                                                     <  2:1331643/150‑1 (MQ=255)

                                                gTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGcc                                                                                                    >  1:4697381/1‑150 (MQ=255)

                                                    tGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAg                                                                                                <  1:1761763/150‑1 (MQ=255)

                                                        caAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCg                                                                                            >  1:135461/1‑150 (MQ=255)

                                                         aaaGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGACGCCATAGGCCGc                                                                                           >  2:3550913/1‑150 (MQ=255)

                                                         aaaGGAAATGGCCGTGGCCACCAGCCCCAAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGACCGGCACCGAAGCCATAGGCCGc                                                                                           >  2:2679741/1‑150 (MQ=255)

                                                              ccATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAgcgc                                                                                      >  1:4973133/1‑150 (MQ=255)

                                                               cATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCt                                                                                     <  2:1969898/150‑1 (MQ=255)

                                                               cATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCt                                                                                     <  2:1970074/150‑1 (MQ=255)

                                                               cATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCt                                                                                     >  1:3186466/1‑150 (MQ=255)

                                                                aTGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTCCTCCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTg                                                                                    >  2:1413542/1‑150 (MQ=255)

                                                                aTGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTg                                                                                    >  2:3763923/1‑150 (MQ=255)

                                                                    ccGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGc                                                                                >  1:9818/1‑150 (MQ=255)

                                                                     cGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGcc                                                                               >  2:4416085/1‑150 (MQ=255)

                                                                      gTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGccc                                                                              >  2:4429939/1‑150 (MQ=255)

                                                                       tGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCa                                                                             <  2:3530422/150‑1 (MQ=255)

                                                                             ccaGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGc                                                                       <  1:362907/150‑1 (MQ=255)

                                                                              caGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCt                                                                      >  2:894845/1‑150 (MQ=255)

                                                                                   cccAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCa                                                                 <  2:699354/150‑1 (MQ=255)

                                                                                         tGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcg                                                           >  2:1779660/1‑150 (MQ=255)

                                                                                              aTGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcgggcgg                                                      >  1:1137955/1‑150 (MQ=255)

                                                                                                   ccatgTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGaa                                                 >  2:1908719/5‑150 (MQ=255)

                                                                                                   aCGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGaa                                                 <  2:1480093/150‑1 (MQ=255)

                                                                                                   aCGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGaa                                                 <  1:2073784/150‑1 (MQ=255)

                                                                                                    cGGGTCAGCCACAGCCGGGCACCGACCTGGTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAg                                                <  1:3795543/150‑1 (MQ=255)

                                                                                                     gggTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGc                                               >  1:2924681/1‑150 (MQ=255)

                                                                                                       gTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCa                                             >  1:546014/1‑150 (MQ=255)

                                                                                                         cAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAAt                                           <  1:4468465/150‑1 (MQ=255)

                                                                                                           gCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGt                                         >  1:1149878/1‑150 (MQ=255)

                                                                                                           gCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGt                                         >  1:3743463/1‑150 (MQ=255)

                                                                                                            ccACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGtt                                        >  2:2015879/1‑150 (MQ=255)

                                                                                                             cacaGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTg                                       >  1:4722787/1‑150 (MQ=255)

                                                                                                             cacaGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTg                                       >  1:2877451/1‑150 (MQ=255)

                                                                                                              acaGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGa                                      >  1:4872873/1‑150 (MQ=255)

                                                                                                              acaGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGa                                      >  2:3305804/1‑150 (MQ=255)

                                                                                                               caGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGAc                                     >  2:1127737/1‑150 (MQ=255)

                                                                                                               caGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGAc                                     >  1:781847/1‑150 (MQ=255)

                                                                                                               caGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGAc                                     <  1:643290/150‑1 (MQ=255)

                                                                                                               caGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGAc                                     <  1:2381799/150‑1 (MQ=255)

                                                                                                                    gggCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGt                                >  1:4808877/1‑150 (MQ=255)

                                                                                                                    gggCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGt                                >  1:347823/1‑150 (MQ=255)

                                                                                                                       cACCGACCTTTCGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGACCAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGa                             <  2:970792/150‑1 (MQ=255)

                                                                                                                        aCCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAg                            >  2:3408672/1‑150 (MQ=255)

                                                                                                                        aCCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAg                            >  2:4727412/1‑150 (MQ=255)

                                                                                                                         ccGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAgg                           <  1:5076625/150‑1 (MQ=255)

                                                                                                                               ttttGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTg                     >  1:3685748/1‑150 (MQ=255)

                                                                                                                                    cagcagcaTGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGAt                >  2:4365157/1‑150 (MQ=255)

                                                                                                                                    cagcagcaTGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGAt                <  1:982523/150‑1 (MQ=255)

                                                                                                                                    cagcagcaTGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGAt                >  1:3972716/1‑150 (MQ=255)

                                                                                                                                     agcagcaTGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATg               >  1:883596/1‑150 (MQ=255)

                                                                                                                                     agcagcaTGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATg               >  1:5116549/1‑150 (MQ=255)

                                                                                                                                       cagcaTGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGaa             <  2:4663793/150‑1 (MQ=255)

                                                                                                                                         gcaTGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACa           >  1:4973816/1‑150 (MQ=255)

                                                                                                                                          gctGTTCGAAGTACCTTCGAACATGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACa           >  2:461866/3‑150 (MQ=255)

                                                                                                                                            tGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatga        <  1:385721/150‑1 (MQ=255)

                                                                                                                                             gTTCGAAGTACCTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatgat       >  1:2852890/1‑150 (MQ=255)

                                                                                                                                             gTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATATGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGACAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatgat       >  2:2146701/1‑150 (MQ=255)

                                                                                                                                             gTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatgat       <  2:2571151/150‑1 (MQ=255)

                                                                                                                                              ttCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatgatg      >  2:4827848/1‑150 (MQ=255)

                                                                                                                                               tCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACAtgatga     >  2:2032020/1‑150 (MQ=255)

TGCTTCGGCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACA‑GCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGA  >  NC_002947/5420639‑5420934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`