Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,706,521 C→A 7.3% intergenic (+68/+44) PP_5006 → / ← PP_5007 TetR family transcriptional regulator/polyhydroxyalkanoate granule‑associated protein GA2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,706,5210CA 7.3% 438.8 / 14.7 141intergenic (+68/+44)PP_5006/PP_5007TetR family transcriptional regulator/polyhydroxyalkanoate granule‑associated protein GA2
Reads supporting (aligned to +/- strand):  ref base C (65/65);  new base A (6/5);  total (71/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 144 total aligned reads displayed.

GATGATGCTGGTGGCGCCGCATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCAGCGCTGGCAGCTG  >  NC_002947/5706372‑5706668
                                                                                                                                                     |                                                                                                                                                   
gatgatGCTGGTGGCGCCGCATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCCCTCCTACAAGGTTAGgcgc                                                                                                                                                     >  2:4579248/1‑150 (MQ=255)
        tggtggCGCCGCATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTa                                                                                                                                             <  1:22277/150‑1 (MQ=255)
        tggtggCGCCGCATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTa                                                                                                                                             >  1:37934/1‑150 (MQ=255)
        tggtggCGCCGCATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTa                                                                                                                                             >  1:3245656/1‑150 (MQ=255)
        tggtggCGCCGCATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTa                                                                                                                                             >  1:4566140/1‑150 (MQ=255)
          gtggCGCCGCATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAgg                                                                                                                                           >  2:123174/1‑150 (MQ=255)
            gtcgccgcATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGGGAAGTACCTGGAGGGGTAGGCCTGCGGGAGATGGAGTGTTGCAGCCGACGCATTCGCGGGTAAACCACAACCGACAAGGTTTGGCGCATCGTGTAggcg                                                                                                                                         >  1:2900185/3‑148 (MQ=255)
             gcgcCGCATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGc                                                                                                                                        <  1:1929210/150‑1 (MQ=255)
               gccgcATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCgg                                                                                                                                      >  2:4231899/1‑150 (MQ=255)
               gccgcATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCgg                                                                                                                                      <  1:3004524/150‑1 (MQ=255)
               gccgcATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCgg                                                                                                                                      <  1:3004485/150‑1 (MQ=255)
               gccgcATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCgg                                                                                                                                      <  1:5041962/150‑1 (MQ=255)
               gccgcATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCgg                                                                                                                                      <  1:2808671/150‑1 (MQ=255)
               gccgcATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCgg                                                                                                                                      <  2:4621408/150‑1 (MQ=255)
               gccgcATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCgg                                                                                                                                      <  1:2808581/150‑1 (MQ=255)
               gccgcATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTGGTAGGAGCgg                                                                                                                                      >  2:2035741/1‑150 (MQ=255)
               gccgcATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGATGCGCCAAACCTTGTAGGAGCgg                                                                                                                                      <  1:1008764/150‑1 (MQ=37)
                ccgcATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCggg                                                                                                                                     <  1:4725767/150‑1 (MQ=255)
                ccgcATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCggg                                                                                                                                     <  2:475308/150‑1 (MQ=255)
                ccgcATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCggg                                                                                                                                     >  1:4649847/1‑150 (MQ=255)
                ccgcATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCggg                                                                                                                                     <  1:1666557/150‑1 (MQ=255)
                  gcATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGtt                                                                                                                                   <  1:3361730/150‑1 (MQ=255)
                     tCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACACGGTTTGGCGCATCTTGTAGGAGCGGGTTTAc                                                                                                                                <  2:1188065/150‑1 (MQ=255)
                       tGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTAccc                                                                                                                              >  1:4853625/1‑150 (MQ=255)
                            gcccaggcccGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTAAACCcgctcc                                                                                                                         <  1:3973024/150‑4 (MQ=255)
                               caggcccGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCgaagaa                                                                                                                      <  2:4649847/150‑1 (MQ=255)
                               caggcccGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCAACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCgaagaa                                                                                                                      >  1:1471778/1‑150 (MQ=255)
                                 ggcccGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAgg                                                                                                                    >  2:3971243/1‑150 (MQ=255)
                                        gcggcggcgGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACCAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGAcgcg                                                                                                             >  2:1015907/1‑150 (MQ=255)
                                          ggcggcggAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGt                                                                                                           <  2:2286231/150‑1 (MQ=255)
                                              gagtagcagcTGTCAGTGAAGTACCTGGTGGGGTTGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGTGTAAACGCGCTCCTACAAGTTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAATAATGCTACTCGGTatca                                                                                                       <  2:3225687/146‑1 (MQ=37)
                                               cggAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTatcag                                                                                                      <  1:3217842/150‑1 (MQ=255)
                                                ggAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGGatcaga                                                                                                     >  1:1602663/1‑150 (MQ=255)
                                                   gcagcGGGCCGTGAAGTACCTGGCGGGGTAGGCCTGCTGGCGAGGTCGTGTTGCAGCCGACGCATTGGCGGGTAAACCCGATCCTACACGGTTTGGCGCATCTTGTTGGAGGGGGTTTACCCGCGAAGAAGGCGACGCGGTatcagatca                                                                                                  <  1:2650186/150‑1 (MQ=255)
                                                     cgcTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCCGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAgg                                                                                                <  1:575551/149‑1 (MQ=255)
                                                     agcTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAgg                                                                                                <  2:4725201/150‑1 (MQ=255)
                                                      gcTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAggg                                                                                               <  2:1441651/150‑1 (MQ=255)
                                                            aGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCgg                                                                                         >  1:2042970/1‑150 (MQ=255)
                                                             gTGAAGTACCTGGTGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGt                                                                                        <  1:2358429/150‑1 (MQ=255)
                                                                 aGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCa                                                                                    <  2:4930549/150‑1 (MQ=255)
                                                                 aGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCa                                                                                    <  2:2945814/150‑1 (MQ=255)
                                                                 aGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAAACCCCGCCAACAAGGTTTTGCGCAAATTGTAGGAGCGGGTTTACCCCCGAAGAAGGCGACGCGGGATAAGATAAGGGTACCGGTGCCa                                                                                    >  1:257875/1‑150 (MQ=255)
                                                                   tACCTGGAGGGGTAGGCGTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGt                                                                                  <  2:2327923/150‑1 (MQ=255)
                                                                   tACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATAAGGGTACCGGTGCCAGt                                                                                  >  2:66907/1‑150 (MQ=255)
                                                                            gggTATGCCTGCTGGAGTTGTAGTTTTGCAGCCGACGCTTTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGa                                                                         <  1:4544159/150‑1 (MQ=255)
                                                                              gTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAAACCGCTCCAACAAGGTTTTGCGCATCTTGTAGGAGCGGGTTTACCCGCGAACAAGGCGACGCGGTATAAGATCAGGGTAACCGTGCCAGTAGGTGCCGACg                                                                       >  1:4013400/1‑150 (MQ=255)
                                                                                aGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGc                                                                     >  2:1675393/1‑150 (MQ=255)
                                                                                aGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGc                                                                     >  2:79083/1‑150 (MQ=255)
                                                                                  gCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTGGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCAACGGCGt                                                                   <  1:374856/150‑1 (MQ=255)
                                                                                     tgctgGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAAACCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGtgct                                                                >  2:345595/1‑150 (MQ=255)
                                                                                        tgGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTgctgct                                                             >  2:426890/1‑150 (MQ=255)
                                                                                        tgGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTgctgct                                                             >  2:4501038/1‑150 (MQ=255)
                                                                                        tgGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTgctgct                                                             <  2:4967309/150‑1 (MQ=255)
                                                                                        tgGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCCCACCAACAAGGGTTTGCGCAACTTTTAGGAGCGGGTTTACCCCCGAAGAAAGCGACCCGGTATAAGATAAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTgctgct                                                             >  1:1762845/1‑150 (MQ=255)
                                                                                        tgGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAAACCCCACCAACAACGTTTGGCGCAACATGTAGGAGCCGGTTTACCCCCGAAGAACGCGACCCCGTATCAGAACACGGTACCCGTGCCACTCGGTGCCCACCGCGTgctgct                                                             >  1:4052106/1‑150 (MQ=255)
                                                                                         ggggaTGTCGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGTTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGctgctg                                                            <  1:1845327/147‑1 (MQ=255)
                                                                                         ggAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTTGCGCATCTTGTAGGATCGGGTTTAACCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCCACGGCGTGctgctg                                                            >  2:3151418/1‑150 (MQ=255)
                                                                                         ggAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGctgctg                                                            >  2:3833394/1‑150 (MQ=255)
                                                                                           agaTGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGc                                                          <  2:899423/150‑1 (MQ=255)
                                                                                             ctGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAGGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCg                                                        <  2:576236/149‑1 (MQ=255)
                                                                                              tGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCgg                                                       <  2:2582217/150‑1 (MQ=255)
                                                                                                tAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCgggg                                                     >  2:2858920/1‑150 (MQ=255)
                                                                                                  gtgtTGCAGCCGACGCATTCGCGGGTAACCCCGCTCCTACAAGGTTTGGCGAATCTTGTAGGCGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTg                                                   <  1:1076748/150‑1 (MQ=255)
                                                                                                     ttGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATGTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATAAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGGCGGGGTggcg                                                >  1:4584127/1‑150 (MQ=255)
                                                                                                      tGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTggcgg                                               <  2:919960/150‑1 (MQ=255)
                                                                                                       gCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCGTGTAGGGGCGGGTTTTCCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTggcggc                                              >  2:3850468/1‑150 (MQ=255)
                                                                                                         aGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAg                                            <  2:3245656/150‑1 (MQ=255)
                                                                                                         aGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAg                                            <  2:3713322/150‑1 (MQ=255)
                                                                                                          gCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGGATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGGCGGGGTGGCGGCAGt                                           >  2:1729228/1‑150 (MQ=255)
                                                                                                          gCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGGAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGGGGCGGCAGt                                           >  2:3729972/1‑150 (MQ=255)
                                                                                                             gACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGc                                        <  1:287926/150‑1 (MQ=255)
                                                                                                                  aTTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTg                                   >  1:636756/1‑150 (MQ=255)
                                                                                                                  aTTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGGTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTg                                   >  1:100076/1‑150 (MQ=255)
                                                                                                                   ttCGCGGGTAAACCCGCTCCTACACGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGc                                  <  2:2205634/150‑1 (MQ=255)
                                                                                                                       cgGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGTTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCtgtg                              <  1:1933770/150‑1 (MQ=255)
                                                                                                                        ggttAAACCCGCTCCTACAAGGTTTGGCGAATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTgg                             <  1:4316779/147‑1 (MQ=255)
                                                                                                                        gggTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGTTCTgg                             <  2:3878673/150‑1 (MQ=255)
                                                                                                                         ggTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGt                            <  2:37934/150‑1 (MQ=255)
                                                                                                                           tAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCg                          >  2:4445205/1‑150 (MQ=255)
                                                                                                                             aaCCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCt                        <  2:4333905/150‑1 (MQ=255)
                                                                                                                              aCCCGCTCCTACAAGATGCGCCAAACCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTg                       >  1:4109550/1‑150 (MQ=37)
                                                                                                                               cccGCTCCTCCAAGGTTTGGCGCATGTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGc                      <  1:3971243/150‑1 (MQ=255)
                                                                                                                               cccGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGc                      >  2:4683221/1‑150 (MQ=255)
                                                                                                                                ccGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGGACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCa                     <  2:644455/150‑1 (MQ=255)
                                                                                                                                 cGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAg                    <  1:79083/150‑1 (MQ=255)
                                                                                                                                 cGCTCCTACAAGATGCGCCAAACCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAg                    >  2:3542198/1‑150 (MQ=38)
                                                                                                                                  gCTCCTACAAGATGCGCCAAACCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGTTCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAgg                   >  2:2378112/1‑150 (MQ=37)
                                                                                                                                  gCTCCTACAAGATGCGCCAAACCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAgg                   >  1:2049650/1‑150 (MQ=38)
                                                                                                                                    tCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGgcg                 >  2:2644979/1‑150 (MQ=255)
                                                                                                                                      cTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCa               <  1:1481562/150‑1 (MQ=255)
                                                                                                                                      cTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCa               <  1:4050565/150‑1 (MQ=255)
                                                                                                                                       tACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCAg              >  2:1405200/1‑150 (MQ=255)
                                                                                                                                       tACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCAg              >  1:2274521/1‑150 (MQ=255)
                                                                                                                                       tACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCAg              >  1:4400899/1‑150 (MQ=255)
                                                                                                                                        aCAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCAgc             >  1:584404/1‑150 (MQ=255)
                                                                                                                                         cAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCAgcg            >  1:1781037/1‑150 (MQ=255)
                                                                                                                                         cAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCAgcg            >  2:2976651/1‑150 (MQ=255)
                                                                                                                                              tttGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCAGCGCTGGc       >  2:50831/1‑150 (MQ=255)
                                                                                                                                              tttGGCGCATCGTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCAGCGCTGGc       >  2:2604044/1‑150 (MQ=255)
                                                                                                                                                   cgcATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCAGCGCTGGCAGCTg  <  1:4231899/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GATGATGCTGGTGGCGCCGCATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCTACAAGGTTTGGCGCATCTTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCAGCGCTGGCAGCTG  >  NC_002947/5706372‑5706668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑