BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,738,920	C→T	19.3%	K168K (AAG→AAA)	hutU ←	urocanate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,738,920	0	C	T	19.3%	380.6 / 81.1	176	K168K (AAG→AAA)	hutU	urocanate hydratase
Reads supporting (aligned to +/- strand): ref base C (80/62); new base T (17/17); total (97/79)
Fisher's exact test for biased strand distribution p-value = 5.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 177 total aligned reads displayed.

TGGCCTGCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTT  >  NC_002947/5738775‑5739069

tGGCCTGCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGc                                                                                                                                                   >  1:536930/1‑150 (MQ=255)

   ccTGCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCtt                                                                                                                                                >  2:717014/1‑150 (MQ=255)

     tGCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTc                                                                                                                                              >  1:4678771/1‑150 (MQ=255)

     tGCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTc                                                                                                                                              <  2:3246185/150‑1 (MQ=255)

      gCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCa                                                                                                                                             <  1:3359563/150‑1 (MQ=255)

      gCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCa                                                                                                                                             <  2:176430/150‑1 (MQ=255)

          gtcgACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCt                                                                                                                                         >  2:2793409/1‑150 (MQ=255)

               cGTAGCGGGTTTCCAGGCGGAAGTCGATATGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTgccgc                                                                                                                                    >  2:1513381/1‑150 (MQ=255)

                   gCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTa                                                                                                                                >  1:3853266/1‑150 (MQ=255)

                   gCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTa                                                                                                                                <  1:3629032/150‑1 (MQ=255)

                   gCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTa                                                                                                                                <  2:619097/150‑1 (MQ=255)

                     gggTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGt                                                                                                                              >  1:4462383/1‑150 (MQ=255)

                         ttCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTg                                                                                                                          <  2:3007695/150‑1 (MQ=255)

                          tCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTgg                                                                                                                         >  1:1713037/1‑150 (MQ=255)

                          tCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTgg                                                                                                                         >  1:121230/1‑150 (MQ=255)

                            cAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCg                                                                                                                       >  2:2996710/1‑150 (MQ=255)

                            cAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCg                                                                                                                       >  1:4053989/1‑150 (MQ=255)

                               gCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGAcc                                                                                                                    >  2:288795/1‑150 (MQ=255)

                                cGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCg                                                                                                                   <  2:1896239/150‑1 (MQ=255)

                                     gTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTc                                                                                                              <  1:1093075/150‑1 (MQ=255)

                                        gATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTcgac                                                                                                           >  1:4703853/1‑150 (MQ=255)

                                         aTACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTcgacg                                                                                                          >  2:4392013/1‑150 (MQ=255)

                                          tACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTcgacga                                                                                                         >  1:3300016/1‑150 (MQ=255)

                                           aCGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGaa                                                                                                        <  1:581652/150‑1 (MQ=255)

                                           aCGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGaa                                                                                                        <  2:3728485/150‑1 (MQ=255)

                                            cGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAg                                                                                                       <  2:938329/150‑1 (MQ=255)

                                            cGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAg                                                                                                       >  1:3941424/1‑150 (MQ=255)

                                            cGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAg                                                                                                       <  1:2996710/150‑1 (MQ=255)

                                             ggCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAgg                                                                                                      >  1:203577/1‑150 (MQ=255)

                                              gCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGt                                                                                                     >  2:5034326/1‑150 (MQ=255)

                                               ctctGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGtt                                                                                                    <  1:1792174/150‑1 (MQ=255)

                                                  tgctgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCa                                                                                                 <  2:720866/150‑1 (MQ=255)

                                                  tgctgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCa                                                                                                 <  2:1230032/150‑1 (MQ=255)

                                                     tgGCATTCGTTGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAg                                                                                              <  1:2279824/150‑1 (MQ=255)

                                                        cATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCTGCTTCGACGAAGGTTTCATAGGTg                                                                                           <  1:4588285/150‑1 (MQ=255)

                                                          ttCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGGTTCATAGGTGcc                                                                                         >  2:2584226/1‑150 (MQ=255)

                                                            cGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCt                                                                                       <  1:1870469/150‑1 (MQ=255)

                                                             gATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTg                                                                                      >  1:4091437/1‑150 (MQ=255)

                                                                 ttCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACg                                                                                  >  2:1963768/1‑150 (MQ=255)

                                                                  tCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACACACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGa                                                                                 <  2:1716899/150‑1 (MQ=255)

                                                                   cAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGAt                                                                                >  1:2040838/1‑150 (MQ=255)

                                                                    aGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATg                                                                               >  1:781624/1‑150 (MQ=255)

                                                                    aGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATg                                                                               >  1:519685/1‑150 (MQ=255)

                                                                    aGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATg                                                                               <  2:4856810/150‑1 (MQ=255)

                                                                      gcagcaagcaCCAGCCATGACGGCTGCGAGAGGCTGTGCTCCGCCCATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGcc                                                                             >  1:3777254/3‑150 (MQ=255)

                                                                      cGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGcc                                                                             <  1:3111927/150‑1 (MQ=255)

                                                                       gAGCAAGCCCCAGCCTGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGccc                                                                            <  1:1513530/150‑1 (MQ=255)

                                                                       gAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGccc                                                                            >  2:2061606/1‑150 (MQ=255)

                                                                       cagcaagcaCCAGCCATGACGGCTGCGAGAGGCTGTGCTCCGCCCATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGccc                                                                            <  1:2755689/149‑1 (MQ=255)

                                                                       cagcaagcaCCAGCCATGACGGCTGCGAGAGGCTGTGCTCCGCCCATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGccc                                                                            <  2:3023356/149‑1 (MQ=255)

                                                                       cagcaagcaCCAGCCATGACGGCTGCGAGAGGCTGTGCTCCGCCCATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGccc                                                                            >  2:579571/2‑150 (MQ=255)

                                                                        agcaagcaCCAGCCATGACGGCTGCGAGAGGCTGTGCTCCGCCCATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCt                                                                           >  2:492633/1‑150 (MQ=255)

                                                                        agcaagcaCCAGCCATGACGGCTGCGAGAGGCTGTGCTCCGCCCATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCt                                                                           <  1:1297844/150‑1 (MQ=255)

                                                                        aGCAAGCCCCAGCCAGGGTCGCGGCCTGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAACGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCt                                                                           <  1:2685843/150‑1 (MQ=255)

                                                                        aGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGACGGTTTCATAGGTGCCCTGGACGATGCCCt                                                                           >  2:82573/1‑150 (MQ=255)

                                                                        aGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCt                                                                           <  1:1942232/150‑1 (MQ=255)

                                                                        aGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCt                                                                           >  2:2478229/1‑150 (MQ=255)

                                                                         gCAAGCACCAGCCATGACGGCTGCGAGAGGCTGTGCTCCGCCCATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTg                                                                          <  1:4305922/150‑1 (MQ=255)

                                                                          cAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTgg                                                                         <  1:717014/150‑1 (MQ=255)

                                                                          cAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTgg                                                                         >  2:3992768/1‑150 (MQ=255)

                                                                           aaGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGc                                                                        >  2:4477341/1‑150 (MQ=255)

                                                                           aaGCACCAGCCATGACGGCTGCGAGAGGCTGTGCTCCGCCCATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTgtg                                                                        >  2:604982/1‑148 (MQ=255)

                                                                              ccccAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGc                                                                     <  1:3123604/150‑1 (MQ=255)

                                                                              ccccAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGc                                                                     >  2:933880/1‑150 (MQ=255)

                                                                                ccagccagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCg                                                                   >  1:3568342/1‑150 (MQ=255)

                                                                                ccagccagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCAGAGGTGCCCTGGACGATGCCCTGGCTGCCg                                                                   >  1:1649348/1‑150 (MQ=255)

                                                                                 cagccagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGa                                                                  >  1:3725533/1‑150 (MQ=255)

                                                                                   gccagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATg                                                                >  1:1126304/1‑150 (MQ=255)

                                                                                   gccagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATg                                                                >  1:3455139/1‑150 (MQ=255)

                                                                                     cagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTa                                                              <  2:1361872/150‑1 (MQ=255)

                                                                                          tCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATcc                                                         <  1:4589827/150‑1 (MQ=255)

                                                                                              ggCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCt                                                     >  1:4224977/1‑150 (MQ=255)

                                                                                                 cAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGcc                                                  >  1:1843757/1‑150 (MQ=255)

                                                                                                     ggCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCcggcg                                              >  2:603529/1‑150 (MQ=255)

                                                                                                        tgtgctccgccCATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTc                                           >  1:5003637/7‑150 (MQ=255)

                                                                                                          ggCGCCGCCCATGCCGCTCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCAt                                         <  2:4609447/150‑1 (MQ=255)

                                                                                                           gcgcCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATc                                        <  1:2199744/150‑1 (MQ=255)

                                                                                                           gcgcCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATc                                        <  1:3237069/150‑1 (MQ=255)

                                                                                                           gcgcCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATc                                        <  1:1513381/150‑1 (MQ=255)

                                                                                                            ctccgccCATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCt                                       <  2:4062053/148‑1 (MQ=255)

                                                                                                             tccgccCATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCtc                                      >  1:2476657/2‑149 (MQ=255)

                                                                                                                gccCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGcc                                   >  2:2966482/1‑150 (MQ=255)

                                                                                                                gccCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGcc                                   <  1:433059/150‑1 (MQ=255)

                                                                                                                gccCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGcc                                   >  1:2502730/1‑150 (MQ=255)

                                                                                                                   cATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATa                                >  2:4228754/1‑150 (MQ=255)

                                                                                                                       ccgccAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACAtg                            <  1:4123231/150‑2 (MQ=255)

                                                                                                                        cgccAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACAtgg                           <  2:4579200/150‑3 (MQ=255)

                                                                                                                         gccCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGc                          >  1:3921583/1‑150 (MQ=255)

                                                                                                                         gccAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGc                          <  2:2105876/150‑1 (MQ=255)

                                                                                                                          ccaaGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGcg                         >  1:3328161/4‑149 (MQ=255)

                                                                                                                          ccaaGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGcg                         >  1:3328276/4‑149 (MQ=255)

                                                                                                                           ccAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCa                        <  1:1963768/150‑1 (MQ=255)

                                                                                                                              ggCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAgac                     >  1:4352916/1‑148 (MQ=255)

                                                                                                                              agCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGc                     >  1:828071/2‑150 (MQ=255)

                                                                                                                                 caccGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCt                  <  1:358130/147‑1 (MQ=255)

                                                                                                                                 cAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCt                  >  1:4385838/1‑150 (MQ=255)

                                                                                                                                 cAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCt                  >  2:3549306/1‑150 (MQ=255)

                                                                                                                                      gTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCg             >  2:4525609/1‑150 (MQ=255)

                                                                                                                                                aCTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGt   >  2:3537798/1‑150 (MQ=255)

                                                                                                                                                 cTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGtt  <  2:4220419/150‑1 (MQ=255)

TGGCCTGCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTT  >  NC_002947/5738775‑5739069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`