Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,743,873 A→G 5.0% F87L (TTC→CTC)  PP_5039 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,743,8730AG 5.0% 511.3 / 9.8 159F87L (TTC→CTC) PP_5039hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (84/67);  new base G (2/6);  total (86/73)
Fisher's exact test for biased strand distribution p-value = 1.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01

Only 100 of 161 total aligned reads displayed.

CGCTCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAGCGCATGGGCGTCGG  >  NC_002947/5743725‑5744022
                                                                                                                                                    |                                                                                                                                                     
cGCTCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAg                                                                                                                                                      <  2:1810555/150‑1 (MQ=255)
   tCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGgcg                                                                                                                                                   >  2:3243279/1‑150 (MQ=255)
     cACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGgcgcg                                                                                                                                                 >  1:4331547/1‑150 (MQ=255)
     cACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGgcgcg                                                                                                                                                 >  1:1486361/1‑150 (MQ=255)
     cACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGgcgcg                                                                                                                                                 >  2:2977119/1‑150 (MQ=255)
        gCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCgcgg                                                                                                                                              >  1:4759245/1‑150 (MQ=255)
         cacaGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCgcggc                                                                                                                                             >  2:4366604/1‑150 (MQ=255)
         cacaGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCgcggc                                                                                                                                             <  1:2301094/150‑1 (MQ=255)
         cacaGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGCGCGCCTTCGAGGGCGAccggc                                                                                                                                             <  1:2679342/150‑1 (MQ=255)
          acaGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCgcggcg                                                                                                                                            >  1:2255953/1‑150 (MQ=255)
           caGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCgt                                                                                                                                           <  2:7231/150‑1 (MQ=255)
           caGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGCGCGCCTTCGAGGGCGACCGGCgt                                                                                                                                           <  1:1774730/150‑1 (MQ=255)
           caGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGACACGCCGCGCGCCTTCGAGGGCGACCGGCgt                                                                                                                                           <  2:1161354/150‑1 (MQ=255)
             ggTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCgtgt                                                                                                                                         >  1:3231038/1‑150 (MQ=255)
              gTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCgcggatggaa                                                                                                                                        >  2:2772585/1‑144 (MQ=255)
                  tGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCgg                                                                                                                                    >  1:5062607/1‑150 (MQ=255)
                   gCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCgtgcccaggt                                                                                                                                   <  2:2145711/150‑8 (MQ=255)
                    ccAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTa                                                                                                                                  <  1:266508/150‑1 (MQ=255)
                    ccAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTa                                                                                                                                  <  1:266334/150‑1 (MQ=255)
                    ccAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTa                                                                                                                                  <  1:3593796/150‑1 (MQ=255)
                    ccAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTa                                                                                                                                  <  2:2446583/150‑1 (MQ=255)
                     cAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAg                                                                                                                                 >  2:3958442/1‑150 (MQ=255)
                     cAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAg                                                                                                                                 <  2:2397854/150‑1 (MQ=255)
                          cAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCAt                                                                                                                            >  2:93217/1‑150 (MQ=255)
                           aGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATg                                                                                                                           >  2:1649954/1‑150 (MQ=255)
                             cACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCg                                                                                                                         >  2:684539/1‑150 (MQ=255)
                              aCTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGt                                                                                                                        <  1:1037379/150‑1 (MQ=255)
                              aCTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGt                                                                                                                        <  1:3929898/150‑1 (MQ=255)
                               cTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTg                                                                                                                       <  2:3594770/150‑1 (MQ=255)
                                ttCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTgg                                                                                                                      <  2:4841009/150‑1 (MQ=255)
                                  cGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCCCCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCa                                                                                                                    >  2:3809412/1‑150 (MQ=255)
                                      aTGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTg                                                                                                                >  2:3477734/1‑150 (MQ=255)
                                         cTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTc                                                                                                             <  1:2086204/150‑1 (MQ=255)
                                          tCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCg                                                                                                            >  2:343966/1‑150 (MQ=255)
                                          tCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGGCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCg                                                                                                            >  1:2995706/1‑150 (MQ=255)
                                           ccAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGa                                                                                                           <  2:3156401/150‑1 (MQ=255)
                                           ccAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGa                                                                                                           <  2:4069693/150‑1 (MQ=255)
                                             aGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGc                                                                                                         <  1:4246288/150‑1 (MQ=255)
                                                    gcagcaCGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATca                                                                                                  >  1:3828668/1‑150 (MQ=255)
                                                     cagcaCGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATcag                                                                                                 <  2:1393106/150‑1 (MQ=255)
                                                     cagcaCGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATcag                                                                                                 <  2:2644141/150‑1 (MQ=255)
                                                      agcaCGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGTAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATcagc                                                                                                <  2:1012630/150‑1 (MQ=255)
                                                         aCGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAgg                                                                                             >  1:3367466/1‑150 (MQ=255)
                                                          cGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCTCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGc                                                                                            <  1:3233924/150‑1 (MQ=255)
                                                           gAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGcc                                                                                           >  2:4297552/1‑150 (MQ=255)
                                                           gAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGcc                                                                                           >  2:840967/1‑150 (MQ=255)
                                                            aaCACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGccc                                                                                          <  2:1808700/150‑1 (MQ=255)
                                                             acacGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCtca                                                                                         >  2:4502780/1‑150 (MQ=255)
                                                             acacGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCcca                                                                                         <  1:3960641/150‑1 (MQ=255)
                                                               acGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCccagc                                                                                       <  2:1971888/150‑1 (MQ=255)
                                                                 gcgcTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGGAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCccagcca                                                                                     >  2:1570423/1‑150 (MQ=255)
                                                                   gcTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCcagccagc                                                                                   >  2:5074860/1‑150 (MQ=255)
                                                                    cTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCagccagcc                                                                                  <  2:2137865/150‑1 (MQ=255)
                                                                       gATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTg                                                                               >  2:4448370/1‑150 (MQ=255)
                                                                       gATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTg                                                                               >  1:3679877/1‑150 (MQ=255)
                                                                         tCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCg                                                                             >  2:3796825/1‑150 (MQ=255)
                                                                         tCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCg                                                                             <  1:1570423/150‑1 (MQ=255)
                                                                         tCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCg                                                                             >  1:4146120/1‑150 (MQ=255)
                                                                           ggCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCggg                                                                           <  2:3768293/150‑1 (MQ=255)
                                                                            gcagcaGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCgggg                                                                          >  2:3602556/1‑150 (MQ=255)
                                                                             cagcagCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGt                                                                         >  2:4943265/1‑150 (MQ=255)
                                                                              agcagcTGGTAGTCCCAGCCCTTCTGCAGGCCCTGTATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGtt                                                                        <  2:1062656/150‑1 (MQ=255)
                                                                              agcagcTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGtt                                                                        <  2:43161/150‑1 (MQ=255)
                                                                               gcagcTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGttt                                                                       >  2:2824294/1‑150 (MQ=255)
                                                                                          tCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCAAACCCCGCAGGgctggctgggcctgc                                                            <  1:547456/150‑8 (MQ=255)
                                                                                           cccAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCtt                                                           >  1:414623/1‑150 (MQ=255)
                                                                                             cAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCg                                                         >  2:3121192/1‑150 (MQ=255)
                                                                                              aGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGa                                                        <  2:1299458/150‑1 (MQ=255)
                                                                                              aGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGa                                                        >  1:4803648/1‑150 (MQ=255)
                                                                                              aGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGa                                                        >  2:4746988/1‑150 (MQ=255)
                                                                                                  cTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGccc                                                    >  1:838169/1‑150 (MQ=255)
                                                                                                   ttCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCa                                                   >  2:3923090/1‑150 (MQ=255)
                                                                                                          ggCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCa                                            <  1:2594453/150‑1 (MQ=255)
                                                                                                          ggCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCa                                            <  2:5112336/150‑1 (MQ=255)
                                                                                                           gCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAg                                           >  1:4376638/1‑150 (MQ=255)
                                                                                                           gCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGGGTCGCGGTAGATCATGCGTGGCAGCTGGGCGAGCAAAATCAGCCGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAg                                           >  1:2756771/1‑150 (MQ=255)
                                                                                                            cccTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAgg                                          <  2:4759245/150‑1 (MQ=255)
                                                                                                             ccTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAggg                                         <  2:2974859/150‑1 (MQ=255)
                                                                                                                gCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGt                                      >  2:1429249/1‑150 (MQ=255)
                                                                                                                    ggCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTc                                  <  1:2256818/150‑1 (MQ=255)
                                                                                                                             cgcgacACGCCGCGCGCCTTCGAGGGCGACCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTtgc                                                                     <  1:1436623/101‑1 (MQ=25)
                                                                                                                             cTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTc                         >  2:543309/1‑150 (MQ=255)
                                                                                                                             cTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTc                         >  1:63434/1‑150 (MQ=255)
                                                                                                                                ggCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGcc                      >  2:4541833/1‑150 (MQ=255)
                                                                                                                                    cGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAAc                  <  2:4653289/150‑1 (MQ=255)
                                                                                                                                     gCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACa                 <  1:4235688/150‑1 (MQ=255)
                                                                                                                                     gCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACa                 >  1:1800858/1‑150 (MQ=255)
                                                                                                                                       cGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAgc               >  1:4842607/1‑150 (MQ=255)
                                                                                                                                       cGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAgc               <  1:1649954/150‑1 (MQ=255)
                                                                                                                                        gcgcGCCTTCGAGGGCGACCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAgcg              <  1:2340043/147‑1 (MQ=255)
                                                                                                                                         gTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAgcgc             <  1:108610/150‑1 (MQ=255)
                                                                                                                                             cccTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAGCGCATgg         >  1:882213/1‑150 (MQ=255)
                                                                                                                                             cccTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAACTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAGCGCATgg         >  1:1825553/1‑150 (MQ=255)
                                                                                                                                              ccTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAGCGCATggg        >  2:98002/1‑150 (MQ=255)
                                                                                                                                              ccTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAGCGCATggg        >  2:4285339/1‑150 (MQ=255)
                                                                                                                                              ccTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAGCGCATggg        >  2:2433160/1‑150 (MQ=255)
                                                                                                                                              ccTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAGCGCATggg        >  2:1515098/1‑150 (MQ=255)
                                                                                                                                              ccTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAGCGCATggg        >  1:4488733/1‑150 (MQ=255)
                                                                                                                                                   aaGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAGCGCATGGGCGTCg   <  1:93217/150‑1 (MQ=255)
                                                                                                                                                    aGGCGCGCGGCGGGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAGCGCATGGGCGTCgg  <  2:913573/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGCTCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAGCGCATGGGCGTCGG  >  NC_002947/5743725‑5744022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑