BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,755,033	C→T	5.2%	A341A (GCC→GCT)	glnA →	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,755,033	0	C	T	5.2%	487.8 / 9.3	153	A341A (GCC→GCT)	glnA	glutamine synthetase
Reads supporting (aligned to +/- strand): ref base C (87/58); new base T (3/5); total (90/63)
Fisher's exact test for biased strand distribution p-value = 2.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

GACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAACAAGATCCACCCAGGCGACGC  >  NC_002947/5754884‑5755182

    ccGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGa                                                                                                                                                   <  2:2922177/150‑1 (MQ=255)

     cGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGAt                                                                                                                                                  >  1:2303692/1‑150 (MQ=255)

     cGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGAt                                                                                                                                                  >  2:927809/1‑150 (MQ=255)

        ccTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCGGCTCGTAACCGTTCCGCTTcctgccg                                                                                                                                               <  1:3597516/150‑7 (MQ=255)

              cTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTcc                                                                                                                                         <  1:627922/150‑1 (MQ=255)

               ttCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCt                                                                                                                                        >  1:2382176/1‑150 (MQ=255)

                   tCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACg                                                                                                                                    >  1:3728937/1‑150 (MQ=255)

                   tCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCGGCTCGTAACCGTTCCGCTTCCTGCCGTATTccattcg                                                                                                                                    <  1:2726341/150‑6 (MQ=255)

                     ggcggTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGtt                                                                                                                                  <  2:2610940/150‑1 (MQ=255)

                      gcggTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTg                                                                                                                                 <  1:3810816/150‑1 (MQ=255)

                             catcaAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGccc                                                                                                                          <  2:1747332/150‑1 (MQ=255)

                              atcaAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCg                                                                                                                         >  1:794497/1‑150 (MQ=255)

                              atcaAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCg                                                                                                                         >  1:1948090/1‑150 (MQ=255)

                               tcaAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGa                                                                                                                        >  1:2964169/1‑150 (MQ=255)

                               tcaAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGa                                                                                                                        >  1:2584009/1‑150 (MQ=255)

                                 aaGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCTCGaaa                                                                                                                      <  1:4331590/150‑1 (MQ=255)

                                 aaGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGaaa                                                                                                                      <  2:140013/150‑1 (MQ=255)

                                 aaGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGaaa                                                                                                                      <  1:1550705/150‑1 (MQ=255)

                                       ggTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGa                                                                                                                <  2:1220456/150‑1 (MQ=255)

                                        gTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGAc                                                                                                               <  1:3194737/150‑1 (MQ=255)

                                         tAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACg                                                                                                              >  1:1838456/1‑150 (MQ=255)

                                         tAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACg                                                                                                              >  1:1838797/1‑150 (MQ=255)

                                               ccTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGa                                                                                                        >  1:140142/1‑150 (MQ=255)

                                                cTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGaa                                                                                                       <  1:696580/150‑1 (MQ=255)

                                                cTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGaa                                                                                                       <  1:1738925/150‑1 (MQ=255)

                                                    aCGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCAc                                                                                                   <  2:4682483/150‑1 (MQ=255)

                                                    aCGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCAc                                                                                                   >  1:1934198/1‑150 (MQ=255)

                                                    aCGGCTTCACCAACCCGTCGACCAACTCGGACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCAc                                                                                                   <  2:1876748/150‑1 (MQ=255)

                                                       gCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCt                                                                                                >  1:4405296/1‑150 (MQ=255)

                                                        cTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCtt                                                                                               >  1:3260861/1‑150 (MQ=255)

                                                        cTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCtt                                                                                               >  2:272607/1‑150 (MQ=255)

                                                         ttCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTc                                                                                              >  1:4563676/1‑150 (MQ=255)

                                                         ttCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCATTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTc                                                                                              >  1:194592/1‑150 (MQ=255)

                                                            aCCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCg                                                                                           >  2:1802063/1‑150 (MQ=255)

                                                            aCCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCg                                                                                           >  2:123767/1‑150 (MQ=255)

                                                            aCCAACCCGTCGACCAACGCGTACCAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCg                                                                                           >  2:2891922/1‑150 (MQ=255)

                                                             ccAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCgg                                                                                          >  2:4905795/1‑150 (MQ=255)

                                                              cAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGa                                                                                         >  2:3333422/1‑150 (MQ=255)

                                                                aCCCGTCGACCCACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGAcc                                                                                       >  1:3746977/1‑150 (MQ=255)

                                                                  ccGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCa                                                                                     >  1:209213/1‑150 (MQ=255)

                                                                       gACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGc                                                                                <  1:913797/150‑1 (MQ=255)

                                                                          cAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCaa                                                                             >  2:1645727/1‑150 (MQ=255)

                                                                          cAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCaa                                                                             >  2:3372754/1‑150 (MQ=255)

                                                                           aaCTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAAc                                                                            >  2:2649486/1‑150 (MQ=255)

                                                                                gTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTa                                                                       >  1:4817472/1‑150 (MQ=255)

                                                                                  aCAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTAcc                                                                     >  2:3638152/1‑150 (MQ=255)

                                                                                   cAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAGGCACGCTTCCCGGACCCATCGGCCAACCCGTACCt                                                                    >  2:2600660/1‑150 (MQ=255)

                                                                                   cAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGCTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACCTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCt                                                                    <  2:4931313/150‑1 (MQ=255)

                                                                                    aaGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTg                                                                   >  2:326515/1‑150 (MQ=255)

                                                                                    aaGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTg                                                                   >  1:585825/1‑150 (MQ=255)

                                                                                      gCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGc                                                                 >  1:3461652/1‑150 (MQ=255)

                                                                                          cTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTc                                                             <  2:2382176/150‑1 (MQ=255)

                                                                                                ccAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGcc                                                       >  1:4751154/1‑150 (MQ=255)

                                                                                                ccAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGcc                                                       >  1:4338098/1‑150 (MQ=255)

                                                                                                  aGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCct                                                     <  1:4290363/150‑1 (MQ=255)

                                                                                                  aGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCct                                                     <  1:4290315/150‑1 (MQ=255)

                                                                                                  aGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCct                                                     >  2:128674/1‑150 (MQ=255)

                                                                                                    gCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCctgc                                                   <  2:1727053/150‑1 (MQ=255)

                                                                                                    gCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCctgc                                                   <  2:2611590/150‑1 (MQ=255)

                                                                                                       tCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGa                                                <  1:448582/150‑1 (MQ=255)

                                                                                                       tCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGa                                                >  2:3425766/1‑150 (MQ=255)

                                                                                                       tCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTAGTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGa                                                <  1:448607/150‑1 (MQ=255)

                                                                                                        cGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGAt                                               >  1:2064057/1‑150 (MQ=255)

                                                                                                         gAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGAtg                                              <  1:416701/150‑1 (MQ=255)

                                                                                                             ccccGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGAtggctg                                          >  1:4583648/1‑150 (MQ=255)

                                                                                                              cccGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGAtggctgg                                         >  1:4918818/1‑150 (MQ=255)

                                                                                                              cccGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGAtggctgg                                         >  2:5011208/1‑150 (MQ=255)

                                                                                                              cccGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGAtggctgg                                         <  1:1101259/150‑1 (MQ=255)

                                                                                                               ccGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGAtggctggc                                        >  1:3279871/1‑150 (MQ=255)

                                                                                                               ccGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGAtggctggc                                        >  2:3346402/1‑150 (MQ=255)

                                                                                                               ccGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGAtggctggc                                        >  1:1277803/1‑150 (MQ=255)

                                                                                                                  gTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTg                                     >  2:1113627/1‑150 (MQ=255)

                                                                                                                  gTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTg                                     >  2:1069541/1‑150 (MQ=255)

                                                                                                                         tGGCCTACTCGGCTCGCAACCGTTCCGACTCGATCCATATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCAGCTGATGGCTGGCCTGGACGGCa                              >  1:1319478/1‑150 (MQ=255)

                                                                                                                            ccTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATcc                           <  1:1140819/150‑1 (MQ=255)

                                                                                                                              tACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAg                         >  2:2412154/1‑150 (MQ=255)

                                                                                                                              tACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAg                         >  2:3235375/1‑150 (MQ=255)

                                                                                                                              tACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAg                         >  2:4642538/1‑150 (MQ=255)

                                                                                                                               aCTCGGCTCGCACCCGGTCCGCCTCGCTCCGGAGTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCGTCCCGGACCCATCGGCCAACCCGGACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGa                        <  2:2964169/150‑1 (MQ=255)

                                                                                                                                cTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGaa                       <  2:2055028/150‑1 (MQ=255)

                                                                                                                                cTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGaa                       <  2:4843381/150‑1 (MQ=255)

                                                                                                                                 tCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAAc                      >  1:1340197/1‑150 (MQ=255)

                                                                                                                                 tCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAAc                      >  1:4966690/1‑150 (MQ=255)

                                                                                                                                   ggCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAACaa                    >  1:1413724/1‑150 (MQ=255)

                                                                                                                                   ggCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAACaa                    <  2:1887770/150‑1 (MQ=255)

                                                                                                                                   ggCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAACaa                    >  2:4735548/1‑150 (MQ=255)

                                                                                                                                     cTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAACAAGa                  <  1:1256511/150‑1 (MQ=255)

                                                                                                                                     cTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAACAAGa                  <  2:1277803/150‑1 (MQ=255)

                                                                                                                                      tCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAACAAGAt                 <  1:3151830/150‑1 (MQ=255)

                                                                                                                                      tCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAACAAGAt                 >  1:2247543/1‑150 (MQ=255)

                                                                                                                                      tCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAACAAGAt                 >  1:2247542/1‑150 (MQ=255)

                                                                                                                                       cGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAACAAGATc                <  2:2303692/150‑1 (MQ=255)

                                                                                                                                       cGCAACCGGTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAACAAGATc                <  2:1939491/150‑1 (MQ=255)

                                                                                                                                          aaCCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAACAAGATCCAc             <  1:5011208/150‑1 (MQ=255)

                                                                                                                                             cGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAACAAGATCCACCCa          <  1:2472470/150‑1 (MQ=255)

                                                                                                                                              gTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAACAAGATCCACCCAg         >  1:336907/1‑150 (MQ=255)

                                                                                                                                               ttCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAACAAGATCCACCCAgg        <  2:2584009/150‑1 (MQ=255)

                                                                                                                                                   gCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAACAAGATCCACCCAGGCGAc    >  1:2029384/1‑150 (MQ=255)

                                                                                                                                                     cTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAACAAGATCCACCCAGGCGAcgc  >  1:2962940/1‑150 (MQ=255)

                                                                                                                                                     cTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAACAAGATCCACCCAGGCGAcgc  >  2:3542711/1‑150 (MQ=255)

GACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGACGGCATCCAGAACAAGATCCACCCAGGCGACGC  >  NC_002947/5754884‑5755182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`