BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,986,915	G→A	6.0%	F61F (TTC→TTT)	ycaC‑II ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,986,915	0	G	A	6.0%	655.3 / 19.1	201	F61F (TTC→TTT)	ycaC‑II	putative hydrolase
Reads supporting (aligned to +/- strand): ref base G (83/104); new base A (6/6); total (90/111)
Fisher's exact test for biased strand distribution p-value = 7.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

Only 100 of 203 total aligned reads displayed.

GATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACGGCGTCGTC  >  NC_002947/5986768‑5987064

gatCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGaa                                                                                                                                                     <  2:4223022/150‑1 (MQ=255)

gatCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGaa                                                                                                                                                     <  1:2368137/150‑1 (MQ=255)

  tCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGc                                                                                                                                                   <  1:2488756/150‑1 (MQ=255)

     gTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtgg                                                                                                                                                <  1:1114095/150‑1 (MQ=255)

     gTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtgg                                                                                                                                                >  2:2384379/1‑150 (MQ=255)

       tgcttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtg                                                                                                                                              <  2:5102406/150‑1 (MQ=255)

       tgcttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtg                                                                                                                                              <  2:2063166/150‑1 (MQ=255)

          ttcttgCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTggtggtc                                                                                                                                           >  1:2429370/6‑149 (MQ=2)

           tgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGa                                                                                                                                          <  1:3071643/150‑1 (MQ=255)

           tcgtgCCGGTGGCCTTCACTGCCTTGACGAAGTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTggtggtca                                                                                                                                          <  2:1225634/146‑3 (MQ=2)

                ccggTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAgaaca                                                                                                                                     <  1:55999/147‑5 (MQ=2)

                   gTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTc                                                                                                                                  <  2:1157140/150‑1 (MQ=255)

                       ccTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCa                                                                                                                              <  1:37140/150‑1 (MQ=255)

                       ccTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCa                                                                                                                              <  1:4781582/150‑1 (MQ=255)

                        cttcactGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAGAACAGTCGGCAg                                                                                                                             >  2:2331175/7‑150 (MQ=2)

                        cTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGATCGGCAg                                                                                                                             >  1:3364330/1‑150 (MQ=255)

                          tGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGc                                                                                                                           <  1:4365405/150‑1 (MQ=255)

                              tGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAGAACAGTCGGCAgcttgaa                                                                                                                       <  1:4486982/150‑7 (MQ=2)

                              tGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTGTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCaaa                                                                                                                       <  1:239685/150‑1 (MQ=255)

                               gCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAg                                                                                                                      <  2:3315299/150‑1 (MQ=255)

                                ccTTGACGAAGTCTTCTTTGTCCCAGGCGTTGATATGGCCGGGACGGGCAATGTAAGGCGCATCCGCGAACATCTCTTTCAGCTCGGGCACCACCGGGCCATTCGGGCCATGCACTAAGCTCGTGGTGAGGATGGTCGGCAGGCCAAAGa                                                                                                                     >  2:3026974/1‑150 (MQ=18)

                                 cTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGaa                                                                                                                    >  1:2125481/1‑150 (MQ=255)

                                 cTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGaa                                                                                                                    >  2:4322015/1‑150 (MQ=255)

                                 cTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGaa                                                                                                                    >  2:3667815/1‑150 (MQ=255)

                                 cTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGaa                                                                                                                    >  2:4322104/1‑150 (MQ=255)

                                  ttGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc                                                                                                                   >  2:1648870/1‑150 (MQ=255)

                                   tGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACt                                                                                                                  >  2:2299170/1‑150 (MQ=255)

                                    gACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt                                                                                                                 <  1:1648870/150‑1 (MQ=255)

                                     aCGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                                                                                                <  2:1394052/150‑1 (MQ=255)

                                     aCGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                                                                                                <  1:2993993/150‑1 (MQ=255)

                                     aCGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                                                                                                <  1:619258/150‑1 (MQ=255)

                                       gAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGc                                                                                                              >  1:1145974/1‑150 (MQ=255)

                                           tCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAgg                                                                                                          >  2:4505916/1‑150 (MQ=255)

                                             ttCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTc                                                                                                        >  1:708472/1‑150 (MQ=255)

                                              tCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCg                                                                                                       >  1:3646848/1‑150 (MQ=255)

                                                gTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGcc                                                                                                     >  1:205030/1‑150 (MQ=255)

                                                   gTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCaa                                                                                                  <  2:246859/150‑1 (MQ=255)

                                                    tCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAg                                                                                                 <  1:2817018/150‑1 (MQ=255)

                                                       cAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCCCCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGcc                                                                                              >  2:1979779/1‑150 (MQ=255)

                                                          gCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGc                                                                                           >  1:1427800/1‑150 (MQ=255)

                                                          gCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGc                                                                                           <  2:4413128/150‑1 (MQ=255)

                                                                   tGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTc                                                                                  >  2:20950/1‑150 (MQ=255)

                                                                   tGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTc                                                                                  >  1:1709553/1‑150 (MQ=255)

                                                                   tGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTc                                                                                  >  1:4289839/1‑150 (MQ=255)

                                                                     gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATTGTCGGCTGGCCAAAGAACTTGGACAGGTCGCCCAAGGCCAGCACGTTGTTCtt                                                                                <  2:2899642/150‑1 (MQ=255)

                                                                     gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt                                                                                <  2:2587197/150‑1 (MQ=255)

                                                                     gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt                                                                                <  2:3488174/150‑1 (MQ=255)

                                                                      ccGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATAGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTg                                                                               <  2:3339180/150‑1 (MQ=255)

                                                                       cGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGa                                                                              >  2:1845545/1‑150 (MQ=255)

                                                                       cGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGa                                                                              >  2:1762841/1‑150 (MQ=255)

                                                                       cGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGa                                                                              <  2:4695719/150‑1 (MQ=255)

                                                                       cGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCCTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGa                                                                              >  1:4539899/1‑150 (MQ=255)

                                                                              ggCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGtt                                                                       <  1:1997437/150‑1 (MQ=255)

                                                                              ggCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGtt                                                                       <  1:2266979/150‑1 (MQ=255)

                                                                              ggCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGtt                                                                       <  2:1201335/150‑1 (MQ=255)

                                                                               gCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTg                                                                      <  2:848909/150‑1 (MQ=255)

                                                                               gCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTg                                                                      <  1:1919584/150‑1 (MQ=255)

                                                                                  aTGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGc                                                                   <  1:4613171/150‑1 (MQ=255)

                                                                                  aTGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGc                                                                   <  2:1544656/150‑1 (MQ=255)

                                                                                   tGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCg                                                                  >  2:1050842/1‑150 (MQ=255)

                                                                                    gTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGa                                                                 >  1:549253/1‑150 (MQ=255)

                                                                                     tACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGaa                                                                <  1:1513163/150‑1 (MQ=255)

                                                                                         gcgcATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAAt                                                            >  2:77401/1‑150 (MQ=255)

                                                                                          cgcATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATc                                                           <  1:2717171/150‑1 (MQ=255)

                                                                                             aTCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCTGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTg                                                        <  2:1133208/150‑1 (MQ=255)

                                                                                             aTCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTg                                                        <  1:315948/150‑1 (MQ=255)

                                                                                             aTCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTg                                                        >  1:2981312/1‑150 (MQ=255)

                                                                                             aTCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTg                                                        >  1:505316/1‑150 (MQ=255)

                                                                                              tCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGc                                                       <  1:1589822/150‑1 (MQ=255)

                                                                                                  ggAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCa                                                   <  2:1217530/150‑1 (MQ=255)

                                                                                                   gAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAg                                                  <  1:2641827/150‑1 (MQ=255)

                                                                                                       aTCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCgag                                              >  1:5039003/1‑150 (MQ=255)

                                                                                                        tctcTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCgaga                                             >  2:3071064/1‑150 (MQ=255)

                                                                                                        tctcTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCgaga                                             <  1:3980801/150‑1 (MQ=255)

                                                                                                        tctcTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCgaga                                             >  1:2604419/1‑150 (MQ=255)

                                                                                                          tctTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATc                                           >  1:1279401/1‑150 (MQ=255)

                                                                                                            tttCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAg                                         >  2:3728076/1‑150 (MQ=255)

                                                                                                             ttCGGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGa                                        <  2:48273/150‑1 (MQ=255)

                                                                                                             ttCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGa                                        <  2:2074901/150‑1 (MQ=255)

                                                                                                              tCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGAc                                       >  1:1213247/1‑150 (MQ=255)

                                                                                                                aGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCg                                     <  2:197023/150‑1 (MQ=255)

                                                                                                                   tCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTc                                  >  1:3175037/1‑150 (MQ=255)

                                                                                                                    cGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCt                                 <  2:3646848/150‑1 (MQ=255)

                                                                                                                     gggCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCtg                                >  2:1796213/1‑150 (MQ=255)

                                                                                                                     gggCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCtg                                <  2:4758533/150‑1 (MQ=255)

                                                                                                                       gCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCtggt                              >  1:4261202/1‑150 (MQ=255)

                                                                                                                        cACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCtggtg                             <  1:4703405/150‑1 (MQ=255)

                                                                                                                           cAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTc                          <  1:358312/150‑1 (MQ=255)

                                                                                                                             gCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGa                        <  2:1098511/150‑1 (MQ=255)

                                                                                                                              cGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGAc                       <  1:2083388/150‑1 (MQ=255)

                                                                                                                                        gggCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGcacc             <  2:2730480/150‑1 (MQ=255)

                                                                                                                                          gCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGcaccac           <  2:5050045/150‑1 (MQ=255)

                                                                                                                                            cTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACgg         >  1:1400096/1‑150 (MQ=255)

                                                                                                                                             ttGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACGGc        <  2:558135/150‑1 (MQ=255)

                                                                                                                                              tGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACGGcg       >  2:1031449/1‑150 (MQ=255)

                                                                                                                                                cTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACGGcgtc     >  2:24213/1‑150 (MQ=255)

                                                                                                                                                 tCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACGGcgtcg    >  1:786249/1‑150 (MQ=255)

                                                                                                                                                 tCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACGGcgtcg    >  1:944668/1‑150 (MQ=255)

                                                                                                                                                 tCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACGGcgtcg    >  2:629369/1‑150 (MQ=255)

                                                                                                                                                   gAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACGGCgtcgtc  >  1:4085619/1‑150 (MQ=255)

GATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACGGCGTCGTC  >  NC_002947/5986768‑5987064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`