BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,992,312	A→G	8.6%	I111I (ATT→ATC)	ycaC‑III ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,992,312	0	A	G	8.6%	584.8 / 28.7	188	I111I (ATT→ATC)	ycaC‑III	putative hydrolase
Reads supporting (aligned to +/- strand): ref base A (104/66); new base G (8/8); total (114/74)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

Only 100 of 189 total aligned reads displayed.

ACCCGCCTGGCTCATGCGGTCATGCGCGGCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTC  >  NC_002947/5992165‑5992461

    gCCTGGCTCATGCGGTCATGCGCGGCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATc                                                                                                                                                 >  2:2249925/1‑150 (MQ=255)

     ccTGGCTCATGCGGTCATGCGCGGCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCa                                                                                                                                                <  2:2719788/150‑1 (MQ=255)

          cTCATGCGGTCATGCGCGGCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAgctgc                                                                                                                                           <  1:1172062/150‑1 (MQ=255)

          cTCATGCGGTCATGCGCGGCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAgctgc                                                                                                                                           >  1:4612934/1‑150 (MQ=255)

           tCATGCGGTCATGCGCGGCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAgctgct                                                                                                                                          >  1:3481206/1‑150 (MQ=255)

           tCATGCGGTCATGCGCGGCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAgctgct                                                                                                                                          >  1:2399601/1‑150 (MQ=255)

           tCATGCGGTCATGCGCGGCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAgctgct                                                                                                                                          >  1:19559/1‑150 (MQ=255)

           tCATGCGGTCATGCGCGGCATCACGGGTCATGTCATGGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCGTCCTCCAAGGCCGACCGCGCCGGGACCGCCACGCAAACCTCGGTCACAACACCTGCCCTGATCCgctgcg                                                                                                                                          >  2:731737/1‑149 (MQ=255)

              tGCGGTCATGCGCGGCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGcttct                                                                                                                                       >  2:4609709/1‑150 (MQ=255)

                 ggTCATGCGCGGCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGc                                                                                                                                    <  2:3959251/150‑1 (MQ=255)

                      tGCGCGGCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTc                                                                                                                               <  1:145463/150‑1 (MQ=255)

                               tCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCAcc                                                                                                                      >  2:1640659/1‑150 (MQ=255)

                                   gggTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCt                                                                                                                  <  2:574332/150‑1 (MQ=255)

                                    ggTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCtt                                                                                                                 <  1:4355885/150‑1 (MQ=255)

                                       catttcattGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGAc                                                                                                              <  2:112871/150‑1 (MQ=255)

                                       catttcattGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGAc                                                                                                              <  1:1113664/150‑1 (MQ=255)

                                         tttcattGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGa                                                                                                            >  2:5113077/1‑150 (MQ=255)

                                         tttcattGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGa                                                                                                            >  2:1667887/1‑150 (MQ=255)

                                         tttcattGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGa                                                                                                            >  1:4139123/1‑150 (MQ=255)

                                         tttcattGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGa                                                                                                            >  1:2828244/1‑150 (MQ=255)

                                          ttcattGAAGGCGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGaa                                                                                                           >  1:3095998/1‑150 (MQ=255)

                                            cattGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGAt                                                                                                                                                  >  2:3509690/1‑109 (MQ=255)

                                            cattGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGAt                                                                                                                                                  <  1:3509690/109‑1 (MQ=255)

                                             attGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATc                                                                                                        >  1:2515799/1‑150 (MQ=255)

                                              ttGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATcc                                                                                                       >  1:70695/1‑150 (MQ=255)

                                              ttGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTGCTTGCCGGTCGCCTTCACCGCCTTGACGaagccc                                                                                                       >  2:3712819/1‑146 (MQ=255)

                                                gAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCGTTCACCGCCTTGACGAAATCCTc                                                                                                     <  2:3558478/150‑1 (MQ=255)

                                                gAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTc                                                                                                     <  2:2412027/150‑1 (MQ=255)

                                                 aaGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCg                                                                                                    >  1:2042452/1‑150 (MQ=255)

                                                  aGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGt                                                                                                   >  1:621005/1‑150 (MQ=255)

                                                  aGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGt                                                                                                   >  1:2806432/1‑150 (MQ=255)

                                                   ggTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGtt                                                                                                  <  1:2576254/150‑1 (MQ=255)

                                                   ggTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGtt                                                                                                  >  2:1689583/1‑150 (MQ=255)

                                                    gTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTg                                                                                                 >  1:3318519/1‑150 (MQ=255)

                                                    gTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTg                                                                                                 >  2:960943/1‑150 (MQ=255)

                                                    gTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTg                                                                                                 >  2:3627082/1‑150 (MQ=255)

                                                        cGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTccc                                                                                             <  1:2871079/150‑1 (MQ=255)

                                                        cGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTccc                                                                                             >  2:4170731/1‑150 (MQ=255)

                                                          gAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCATCGCCTTGACGAAATCCTCGTTGTCCCAg                                                                                           <  2:2630553/150‑1 (MQ=255)

                                                          gAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAg                                                                                           >  2:1187953/1‑150 (MQ=255)

                                                             gCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCg                                                                                        <  1:1497434/150‑1 (MQ=255)

                                                              cATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGt                                                                                       <  1:3192364/150‑1 (MQ=255)

                                                               aTCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGtt                                                                                      >  1:3397109/1‑150 (MQ=255)

                                                                   gTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATc                                                                                  <  1:2025057/150‑1 (MQ=255)

                                                                    tCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCt                                                                                 >  2:2742075/1‑150 (MQ=255)

                                                                     caccacGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTg                                                                                >  1:3172886/1‑150 (MQ=255)

                                                                       ccacGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGc                                                                              <  1:587320/150‑1 (MQ=255)

                                                                       ccacGAACACCTCAAATTCGTCCTCCCAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCGTCTGGCCGGGCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGc                                                                              <  2:2804597/150‑1 (MQ=25)

                                                                        cacGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGcc                                                                             >  1:482452/1‑150 (MQ=255)

                                                                         acGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCa                                                                            <  1:661103/150‑1 (MQ=255)

                                                                               acCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAgggcgg                                                                      <  2:3423474/150‑1 (MQ=255)

                                                                                 cTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAgggcgggc                                                                    >  1:3109222/1‑150 (MQ=255)

                                                                                 cTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAACGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAgggcgggc                                                                    >  1:4261693/1‑150 (MQ=255)

                                                                                  tCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCa                                                                   >  1:1859060/1‑150 (MQ=255)

                                                                                  tCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCa                                                                   >  2:2576029/1‑150 (MQ=255)

                                                                                  tCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCa                                                                   >  1:3749577/1‑150 (MQ=255)

                                                                                    aaaTTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAAt                                                                 >  2:2334684/1‑150 (MQ=255)

                                                                                    aaaTTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAAt                                                                 >  2:4921796/1‑150 (MQ=255)

                                                                                       ttcttcCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTa                                                              <  1:2249904/150‑1 (MQ=255)

                                                                                        tcttcCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTAc                                                             <  1:4272810/150‑1 (MQ=255)

                                                                                          ttcCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACgg                                                           >  2:1350092/1‑150 (MQ=255)

                                                                                           tcctccAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGgc                                                          >  2:4964537/1‑150 (MQ=255)

                                                                                           tcctccAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGgc                                                          <  1:4681515/150‑1 (MQ=255)

                                                                                              tccAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCa                                                       >  1:3636841/1‑150 (MQ=255)

                                                                                              tccAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCa                                                       >  1:3969248/1‑150 (MQ=255)

                                                                                              tccAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCa                                                       >  2:1211879/1‑150 (MQ=255)

                                                                                              tccAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCa                                                       >  1:3083684/1‑150 (MQ=255)

                                                                                                cAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATc                                                     >  1:2278039/1‑150 (MQ=255)

                                                                                                  aGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCg                                                   <  2:4824123/150‑1 (MQ=255)

                                                                                                   ggCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCgg                                                  <  2:2649143/150‑1 (MQ=255)

                                                                                                      ggacaatgcCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGaa                                               >  1:133503/8‑150 (MQ=14)

                                                                                                       gACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAAc                                              <  2:478176/150‑1 (MQ=255)

                                                                                                        aCAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACa                                             >  2:2259552/1‑150 (MQ=255)

                                                                                                           atgcCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGaatgttt                                          >  2:1873592/3‑145 (MQ=14)

                                                                                                            tgcCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGaatgtttc                                         <  2:2996529/149‑7 (MQ=14)

                                                                                                             gcCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGaatgtttct                                        <  2:2247073/150‑8 (MQ=14)

                                                                                                                 ggAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGACCAGGGCTTTCa                                    >  2:565909/1‑150 (MQ=255)

                                                                                                                      gCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTcc                               >  2:1384170/1‑150 (MQ=255)

                                                                                                                      gCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTcc                               >  1:4926086/1‑150 (MQ=255)

                                                                                                                       ccACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCg                              <  1:3292405/150‑1 (MQ=255)

                                                                                                                               aCCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGc                      >  1:1212033/1‑150 (MQ=255)

                                                                                                                               aCCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGc                      >  2:4616406/1‑150 (MQ=255)

                                                                                                                                  tCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCaatggg                   <  1:293240/150‑6 (MQ=9)

                                                                                                                                  tCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTGGACGAAATCCTCGTTGTCCCAGGCGTGGATCGGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTATCAGGTCCGGCCCCAGCggg                   >  2:2395636/1‑150 (MQ=18)

                                                                                                                                      tCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACAGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGt               >  1:4840341/1‑150 (MQ=255)

                                                                                                                                       cacaACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCAtt              <  1:323039/150‑1 (MQ=9)

                                                                                                                                        acaacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCa                                                                            <  1:2742802/87‑1 (MQ=255)

                                                                                                                                        acaacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTg             >  1:1809837/1‑150 (MQ=255)

                                                                                                                                        acaacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTg             >  2:2837675/1‑150 (MQ=255)

                                                                                                                                          aacaCCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTcgg           >  1:2708495/1‑150 (MQ=2)

                                                                                                                                          aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTggg           <  1:369671/150‑1 (MQ=255)

                                                                                                                                               cTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGccgt      >  2:683525/1‑148 (MQ=2)

                                                                                                                                               cTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCtt      >  1:2626871/1‑150 (MQ=255)

                                                                                                                                               cTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCtt      <  2:19559/150‑1 (MQ=255)

                                                                                                                                               cTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCtt      >  1:3761208/1‑150 (MQ=255)

                                                                                                                                               cTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCtt      <  2:2399510/150‑1 (MQ=255)

                                                                                                                                                tGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTg     >  2:4419908/1‑150 (MQ=255)

                                                                                                                                                 gCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGc    <  2:2004318/150‑1 (MQ=255)

                                                                                                                                                  cAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCt   >  1:1655369/1‑150 (MQ=255)

                                                                                                                                                   aaTGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTc  >  2:1447982/1‑150 (MQ=255)

ACCCGCCTGGCTCATGCGGTCATGCGCGGCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTC  >  NC_002947/5992165‑5992461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`