BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	514,927	G→T	5.7%	L54M (CTG→ATG) ‡	PP_0426 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	514,927	0	G	T	5.7%	459.3 / 12.9	140	L54M (CTG→ATG) ‡	PP_0426	membrane protein
Reads supporting (aligned to +/- strand): ref base G (70/61); new base T (5/3); total (76/64)
Fisher's exact test for biased strand distribution p-value = 7.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

Only 100 of 141 total aligned reads displayed.

GCCAGCAGCGACGGGCTGGACGCGGCGTTGCAGATGGAGGGCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCACGGCGCGCGCCAGGTCCTTGGCGTCGAAT  >  NC_002947/514778‑515075

 ccAGCAGCGACGGGCTGGACGCGGCGTTGCAGATGGAGGGCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGc                                                                                                                                                     >  1:3284795/1‑150 (MQ=255)

         gACGGGCTGGACGCGGCGTTGCAGATGGAGGGCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGcc                                                                                                                                             >  2:4364760/1‑150 (MQ=255)

           cGGGCTGGACGCGGCGTTGCAGATGGAGGGCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATCCCTCGGTGAGCAACCAGCCCATTGCCGCCATGCTGATCATGGCGGCaa                                                                                                                                           <  2:497704/150‑1 (MQ=255)

            gggCTGGACGCGGCGTTGCAGATGGAGGGCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGGCGCCATGCTGATCATGGCGGCaat                                                                                                                                          <  1:3441302/150‑2 (MQ=255)

            gggCTGGACGCGGCGTTGCAGATGGAGGGCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCaaa                                                                                                                                          <  1:699009/150‑1 (MQ=255)

            gggCTGGACGCGGCGTTGCAGATGGAGGGCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCaaa                                                                                                                                          <  1:292818/150‑1 (MQ=255)

            gggCTGGACGCGGCGTTGCAGATGGAGGGCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCaaa                                                                                                                                          <  1:1958664/150‑1 (MQ=255)

            gggCTGGACGCGGCGTTGCAGATGGAGGGCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTG‑CGCCATGCTGATCATGGCGGCaatg                                                                                                                                         <  2:1307466/150‑3 (MQ=255)

               cTGGACGCGGCGTTGCAGATGGAGGGCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTa                                                                                                                                       >  1:1484494/1‑150 (MQ=255)

                tGGACGCGGCGTTGCAGATGGAGGGCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAg                                                                                                                                      >  2:920895/1‑150 (MQ=255)

                tGGACGCGGCGTTGCAGATGGAGGGCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAg                                                                                                                                      >  1:966206/1‑150 (MQ=255)

                    cgcgGCGTTGCAGATGGAGGGCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAgg                                                                                                                                  <  2:3925713/150‑1 (MQ=255)

                    cgcgGCGTTGCAGATGGAGGGCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAgg                                                                                                                                  <  2:1346070/150‑1 (MQ=255)

                          gTTGCAGATGGAGGGCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACAt                                                                                                                            <  1:1562951/150‑1 (MQ=255)

                            tGCAGATGGAGGGCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATgg                                                                                                                          <  1:2500322/150‑1 (MQ=255)

                                gATGGAGGGCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCa                                                                                                                      <  2:4701725/150‑1 (MQ=255)

                                    gAGGGCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTg                                                                                                                  >  2:2913982/1‑150 (MQ=255)

                                      gggCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGaa                                                                                                                >  2:745481/1‑150 (MQ=255)

                                       ggCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCACAGTAGAAGGCAACATGGGCCAGCTGaca                                                                                                               >  2:3714727/1‑148 (MQ=255)

                                       ggCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGaaa                                                                                                               >  2:1122569/1‑150 (MQ=255)

                                       ggCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGaaa                                                                                                               <  2:4017511/150‑1 (MQ=255)

                                         cGAACAAGGTGATCAGCAGGATATAGAGGCCGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCAAGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGc                                                                                                             >  1:12145/1‑150 (MQ=255)

                                          gAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCt                                                                                                            >  2:4234369/1‑150 (MQ=255)

                                              aaGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCa                                                                                                        >  2:2524313/1‑150 (MQ=255)

                                                ggTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACg                                                                                                      >  1:1466248/1‑150 (MQ=255)

                                                ggTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACg                                                                                                      >  2:738177/1‑150 (MQ=255)

                                                 gTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCCAGGCGCCAAAGTAGAAGGCAACAAGGGCCAGCTGAAAGCTGCCACgt                                                                                                     >  1:1846495/1‑150 (MQ=255)

                                                 gTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACgt                                                                                                     <  2:2666650/150‑1 (MQ=255)

                                                      cagcagGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGc                                                                                                <  1:784880/150‑1 (MQ=255)

                                                        gcagGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCgg                                                                                              <  1:4743984/150‑1 (MQ=255)

                                                         cagGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCggg                                                                                             <  1:2913982/150‑1 (MQ=255)

                                                          agGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGGGCGGGt                                                                                            <  1:3889719/150‑1 (MQ=255)

                                                           ggATATAGAGGCTGGAGATGATCAACAGCGCGCCGTCACCGAGGCTCCTCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCCACAAGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGtt                                                                                           >  2:4442542/1‑150 (MQ=255)

                                                            gATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTg                                                                                          >  2:2436853/1‑150 (MQ=255)

                                                               ataGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTg                                                                                       <  2:2991710/150‑1 (MQ=255)

                                                               ataGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTg                                                                                       <  1:2347517/150‑1 (MQ=255)

                                                                  gagGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGgcg                                                                                    >  2:3234455/1‑150 (MQ=255)

                                                                    ggCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCa                                                                                  <  1:1208894/150‑1 (MQ=255)

                                                                     gcgggCGATGAACAACAGCGCGCCGTCCCCGAGGCTCATCCAAGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGACGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAg                                                                                 <  1:4276956/147‑1 (MQ=255)

                                                                       tGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGACAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGa                                                                               >  2:812617/1‑150 (MQ=255)

                                                                       tGGAGATGATCAACAGCGCGCCGTCACAGAGGCTCATCCATGCCTCCGTGAGCAACCAGCCCAATTCCCCCATGAAAACCAATGCGCCAAAGAAGAAGGCAACATGGGCCCGCAGAAAGCTGCCACGAGGTGCGGGTTGCTGGCGCAGGa                                                                               >  1:4359008/1‑150 (MQ=255)

                                                                         gCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCATAGTTGAAGGCAACATGGGCCATCTTAAAGCTGCCTCGTGGTGCGGGTTGCTTGCGCATGAAt                                                                             <  2:2634298/150‑1 (MQ=255)

                                                                            atgatCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGcc                                                                          <  1:1091687/150‑1 (MQ=255)

                                                                                tCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACa                                                                      >  2:2332263/1‑150 (MQ=255)

                                                                                tCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACa                                                                      >  2:1396566/1‑150 (MQ=255)

                                                                                  aaCAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAgg                                                                    >  2:4428251/1‑150 (MQ=255)

                                                                                  aaCAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATACCAACAgg                                                                    <  1:4554780/150‑1 (MQ=255)

                                                                                   aCAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCTTGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGc                                                                   >  2:3682606/1‑150 (MQ=255)

                                                                                       cgcgcCGTCACCGAGGCTCATCCATGCCTCTGTGAGGAACCAGCGCAGAGCCGCCATGATCAGCACGGCGCCAAAGCAGAAGGCTACTTGGGCCATCGTAAAGCTGCCACGTGTTTCGGGTTGGTGGCGCAGGAATGCCAACAGTCTTTg                                                               <  1:1387243/150‑1 (MQ=255)

                                                                                       cgcgcCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTg                                                               <  1:1387250/150‑1 (MQ=255)

                                                                                        gcgcCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTgg                                                              <  1:5023686/150‑1 (MQ=255)

                                                                                          gcCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTc                                                            <  2:4583788/150‑1 (MQ=255)

                                                                                          gcCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAAACAGCCCATTGCCGCCATGATCAGCATTGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTTGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTc                                                            >  2:1275604/1‑150 (MQ=255)

                                                                                              tCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCtt                                                                 <  1:4117790/141‑1 (MQ=255)

                                                                                              tCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTcctgg                                                        >  1:2486558/1‑150 (MQ=255)

                                                                                                aCCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTTCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACAAGGGCCAGCTTAAAGATGCCACGTGGTGCGGGTTGCTGGCGCAGGAATTCAAACAGGCTTTGGTcctgtcc                                                      >  1:4718572/1‑150 (MQ=255)

                                                                                                aCCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTcctggcc                                                      >  1:2550490/1‑150 (MQ=255)

                                                                                                  cGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGTCTGCCAACAGGCGGTGGTcctggcgtg                                                    >  2:4094260/1‑150 (MQ=255)

                                                                                                      gCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTg                                                <  2:3326787/150‑1 (MQ=255)

                                                                                                        tCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGga                                              <  2:1484494/150‑1 (MQ=255)

                                                                                                        tCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGga                                              >  1:575157/1‑150 (MQ=255)

                                                                                                        tCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGga                                              <  2:2315312/150‑1 (MQ=255)

                                                                                                           tCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGaa                                           >  2:2541006/1‑150 (MQ=255)

                                                                                                           tCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGaa                                           >  1:1915424/1‑150 (MQ=255)

                                                                                                           tCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGaa                                           >  2:1074442/1‑150 (MQ=255)

                                                                                                            atgagCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAAt                                          <  1:3065800/146‑1 (MQ=255)

                                                                                                             cATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGTTCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATg                                         <  1:1926124/150‑1 (MQ=255)

                                                                                                             cATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAAAGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATg                                         >  1:2388258/1‑150 (MQ=255)

                                                                                                              aggCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGc                                        <  2:4930119/148‑1 (MQ=255)

                                                                                                              aTGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGc                                        <  1:920895/150‑1 (MQ=255)

                                                                                                                gCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCt                                      <  1:2665566/150‑1 (MQ=255)

                                                                                                                 ccTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTg                                     >  2:2191917/1‑150 (MQ=255)

                                                                                                                  cTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGc                                                                                                                                 >  1:4734444/1‑57 (MQ=255)

                                                                                                                  cTCGGTGAGCAACCAGCCCATGGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGc                                    <  2:3347611/150‑1 (MQ=255)

                                                                                                                   tCGGTGAGCAACCAGCCCATTGCCGCCATGATCGGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTGTGGTCCTGGCCGGGGTGGAGAGTGCCTGCt                                   >  2:2022114/1‑150 (MQ=255)

                                                                                                                   tCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCt                                   >  1:1875402/1‑150 (MQ=255)

                                                                                                                    cGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCtt                                  <  1:2329971/150‑1 (MQ=255)

                                                                                                                    cGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCtt                                  <  1:2226212/150‑1 (MQ=255)

                                                                                                                       tGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCa                               >  2:1470384/1‑150 (MQ=255)

                                                                                                                       tGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCa                               >  2:4050368/1‑150 (MQ=255)

                                                                                                                       tGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCa                               >  2:4050182/1‑150 (MQ=255)

                                                                                                                         aGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCACg                             >  2:22982/1‑150 (MQ=255)

                                                                                                                           cAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCACGgc                           <  2:3563042/150‑1 (MQ=255)

                                                                                                                           cAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCACGgc                           <  2:3562825/150‑1 (MQ=255)

                                                                                                                              ccAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCACGgcgac                        >  2:1825729/1‑148 (MQ=255)

                                                                                                                               cAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCACGgcgcgc                       >  1:4262617/1‑150 (MQ=255)

                                                                                                                               cAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCACGgcgcgc                       >  2:2831840/1‑150 (MQ=255)

                                                                                                                                tctactttGGCGCCATGCTGATCATGGCGGCAATGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCGTGGTTGGAGAATGCCTGCTTGCACGgcgcgcg                      >  2:4182075/7‑150 (MQ=255)

                                                                                                                                 gCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCACGgcgcgcgc                     >  1:3253106/1‑150 (MQ=255)

                                                                                                                                     tttGGCGCCATGCTGATCATGGCGGCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCACGGCGCGCGCCAgg                 >  2:3797354/2‑150 (MQ=255)

                                                                                                                                     tttGGCGCCATGCTGATCATGGCGGCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCACGGCGCGCGCCAgg                 >  2:244782/2‑150 (MQ=255)

                                                                                                                                     tttGGCGCCATGCTGATCATGGCGGCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCACGGCGCGCGCCAgg                 >  1:2474119/2‑150 (MQ=255)

                                                                                                                                      ttGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCACGGCGCGCGCCAGGt                >  2:5119352/1‑150 (MQ=255)

                                                                                                                                         ccgccATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAAt                                                                             <  1:2904889/86‑1 (MQ=255)

                                                                                                                                           gccATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCACGGCGCGCGCCAGGTCCTTg           <  1:324510/150‑1 (MQ=255)

                                                                                                                                           gccATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCACGGCGCGCGCCAGGTCCTTg           <  2:2669776/150‑1 (MQ=255)

                                                                                                                                             cATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCACGGCGCGCGCCAGGTCCTTGGc         <  2:1754999/150‑1 (MQ=255)

                                                                                                                                                gATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCACGGCGCGCGCCAGGTCCTTGGCGTc      <  1:80335/150‑1 (MQ=255)

                                                                                                                                                 aTCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCACGGCGCGCGCCAGGTCCTTGGCGTCg     >  1:3669359/1‑150 (MQ=255)

                                                                                                                                                    aGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCACGGCGCGCGCCAGGTCCTTGGCGTCGAAt  <  2:2801345/150‑1 (MQ=255)

GCCAGCAGCGACGGGCTGGACGCGGCGTTGCAGATGGAGGGCGAACAAGGTGATCAGCAGGATATAGAGGCTGGCGATGATCAACAGCGCGCCGTCACCGAGGCTCATCCATGCCTCGGTGAGCAACCAGCCCATTGCCGCCATGATCAGCATGGCGCCAAAGTAGAAGGCAACATGGGCCAGCTGAAAGCTGCCACGTGGTGCGGGTTGCTGGCGCAGGAATGCCAACAGGCTTTGGTCCTGGCCTGGTTGGAGAATGCCTGCTTGCACGGCGCGCGCCAGGTCCTTGGCGTCGAAT  >  NC_002947/514778‑515075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`