Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 542,004 C→A 5.5% R81S (CGC→AGC) ‡ rpoC → DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947542,0040CA 5.5% 729.0 / 22.0 217R81S (CGC→AGC) ‡rpoCDNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base C (129/76);  new base A (7/5);  total (136/81)
Fisher's exact test for biased strand distribution p-value = 7.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 217 total aligned reads displayed.

GTGGTCGTTCGGTGAAGTTAAGAAGCCGGAAACCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAGTCGCTGCCGTCCCGTATCGGCTTGCTGATGGACA  >  NC_002947/541859‑542151
                                                                                                                                                 |                                                                                                                                                   
  ggTCGTTCGGTGAAGTTAAGAAGCCGGAAACCTTCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTTCGAGTGCCTGTGCGGCTAGTACTAGCGCCTCTAGCACCgcggcg                                                                                                                                               <  2:114452/150‑1 (MQ=255)
      gTTCGGTGAAGTTAAGAAGCCGGAAACCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAAt                                                                                                                                           >  2:3061594/1‑150 (MQ=255)
       ttCGGTGAAGTTAAGAAGCCGGAAACCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATc                                                                                                                                          >  1:2549170/1‑150 (MQ=255)
          ggTGAAGTTAAGAAGCCGGAAACCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGc                                                                                                                                       >  2:4228460/1‑150 (MQ=255)
          ggTGAAGTTAAGAAGCCGGAAACCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGc                                                                                                                                       <  2:4299972/150‑1 (MQ=255)
          ggTGAAGTTAAGAAGCCGGAAACCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGc                                                                                                                                       <  1:4937672/150‑1 (MQ=255)
            tGAAGTTAAGAAGCCGGAAACCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCga                                                                                                                                     <  2:694063/150‑1 (MQ=255)
            tGAAGTTAAGAAGCCGGAAACCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCga                                                                                                                                     <  2:637418/150‑1 (MQ=255)
                gTTAAGAAGCCGGAAACCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAg                                                                                                                                 >  1:373084/1‑150 (MQ=255)
                 ttAAGAAGCCGGAAACCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGt                                                                                                                                >  2:2523413/1‑150 (MQ=255)
                   aagaagCCGGAAACCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTgc                                                                                                                              >  2:550749/1‑150 (MQ=255)
                     gaagCCGGAAACCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTgcgg                                                                                                                            >  1:2094814/1‑150 (MQ=255)
                       agCCGGAAACCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTgcggcg                                                                                                                          >  1:433043/1‑150 (MQ=255)
                        gCCGGAGACGATCAACTATCGTACGTTCAAGCCTGAACGCGATGGTCTCTTCTGCGCGCGTATCTTTGGCCCGATCAAGGATTATGAATGCTTGTGCGGCAAGTACAAGCGTATGAAATACAAAGGCATCATCTGCGAAAAGTGCGGCGt                                                                                                                         >  2:1004642/1‑150 (MQ=255)
                        gCCGGAAACCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGt                                                                                                                         >  1:2809975/1‑150 (MQ=255)
                         ccGGAAACCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGtt                                                                                                                        >  2:1731410/1‑150 (MQ=255)
                         ccGGAAACCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGtt                                                                                                                        <  2:1076013/150‑1 (MQ=255)
                           ggAAACCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGa                                                                                                                      >  2:389619/1‑150 (MQ=255)
                            gAAACCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGaa                                                                                                                     >  1:2452419/1‑150 (MQ=255)
                               aCCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGtt                                                                                                                  <  2:4167378/150‑1 (MQ=255)
                               aCCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGtt                                                                                                                  <  2:1681897/150‑1 (MQ=255)
                                 gaTCAACTATCGTACGTTCAAGCCTGAACGCGATGGTCTCTTCTGCGCGCGTATCTTTGGCCCGATCAAGGATTATGAATGCTTGTGCGGCAAGTACAAGCGTATGAAATACAAAGGCATCATCTGCGAAAAGTGCGGCGTTGAAGtcac                                                                                                                <  2:2311651/149‑4 (MQ=255)
                                 cATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGc                                                                                                                >  2:4594766/1‑150 (MQ=255)
                                 cATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGc                                                                                                                >  2:4549246/1‑150 (MQ=255)
                                  aTCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGcc                                                                                                               >  2:4112756/1‑150 (MQ=255)
                                  aTCAACTACCGTACGTTAAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGcc                                                                                                               >  2:4878692/1‑150 (MQ=255)
                                        tACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGCGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCg                                                                                                         >  2:2948801/1‑150 (MQ=255)
                                          ccGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGaa                                                                                                       <  1:2344353/150‑1 (MQ=255)
                                           cGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAg                                                                                                      <  1:3083829/150‑1 (MQ=255)
                                             catcagcAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGt                                                                                                    >  2:3350308/7‑150 (MQ=255)
                                                  tCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTtcgtc                                                                                               <  1:1728469/150‑1 (MQ=255)
                                                   cAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTtcgtcg                                                                                              >  2:3291651/1‑150 (MQ=255)
                                                   cAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTtcgtcg                                                                                              >  1:2420117/1‑150 (MQ=255)
                                                      gCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGa                                                                                           >  1:4162339/1‑150 (MQ=255)
                                                      gCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGa                                                                                           >  1:1089079/1‑150 (MQ=255)
                                                         tGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAgcg                                                                                        >  2:2149021/1‑150 (MQ=255)
                                                           aGCGTGACGGCCTGATCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTAAGAGTGACTGAGCGGCAAGTACAAGCGCCTCAACCACCGCGGCGAAATCTGCTAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGATCGTCGTGAGCGCa                                                                                      >  2:319419/1‑150 (MQ=255)
                                                                 aCGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGgcgc                                                                                >  1:66596/1‑150 (MQ=255)
                                                                      cTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATc                                                                           >  1:2839168/1‑150 (MQ=255)
                                                                      cTGTTCTGCGCCAAGATCATTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATc                                                                           <  1:4048740/150‑1 (MQ=255)
                                                                         ttCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAg                                                                        <  2:3938109/150‑1 (MQ=255)
                                                                           cTGCGCCACGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCt                                                                      <  2:436807/150‑1 (MQ=255)
                                                                           cTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCt                                                                      <  2:128988/150‑1 (MQ=255)
                                                                           cTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCt                                                                      <  2:1916581/150‑1 (MQ=255)
                                                                            tGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTg                                                                     <  2:618695/150‑1 (MQ=255)
                                                                            tGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTg                                                                     <  1:389619/150‑1 (MQ=255)
                                                                              cgcgcgtaTCTTTGGCCCGATCAAGGATTATGAATGCTTGTGCGGCAAGTACAAGCGTATGAAATACAAAGGCATCATCTGCGAAAAGTGCGGCGTTGAAGTCACGCTGTCGCGCGTTCGTCGTGAGCGCATGGGCCATATTGAGCTCGc                                                                   <  2:707491/143‑1 (MQ=255)
                                                                              cgcCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGc                                                                   <  1:706692/150‑1 (MQ=255)
                                                                               gcCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGcc                                                                  >  1:4767111/1‑150 (MQ=255)
                                                                                ccAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCt                                                                 >  1:4653515/1‑150 (MQ=255)
                                                                                 cAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTc                                                                >  1:4891896/1‑150 (MQ=255)
                                                                                 cAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTc                                                                >  1:224057/1‑150 (MQ=255)
                                                                                  aaGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCg                                                               >  2:1889494/1‑150 (MQ=255)
                                                                                  aaGATC‑TTGGCGCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGc                                                              >  1:3105908/1‑150 (MQ=255)
                                                                                   aGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGc                                                              <  1:4322894/150‑1 (MQ=255)
                                                                                   aGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGATGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGc                                                              <  2:373084/150‑1 (MQ=255)
                                                                                    gATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGcc                                                             <  1:4233648/150‑1 (MQ=255)
                                                                                        tttGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGtt                                                         >  1:4314795/1‑150 (MQ=255)
                                                                                        tttGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGtt                                                         >  1:435347/1‑150 (MQ=255)
                                                                                        tttGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGtt                                                         >  2:2471811/1‑150 (MQ=255)
                                                                                           ggCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGcc                                                      <  1:4864246/150‑1 (MQ=255)
                                                                                             cccAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTAGCCca                                                    >  1:2944669/1‑150 (MQ=255)
                                                                                              ccAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGTAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCcac                                                   >  2:3569876/1‑150 (MQ=255)
                                                                                               cAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGTGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCcaca                                                  >  2:1623767/1‑150 (MQ=255)
                                                                                                    aaGGATTATGAATGCTTGTGCGGCAAGTACAAGCGTATGAAATACAAAGGCATCATCTGCGAAAAGTGCGGCGTTGAAGTCACGCTGTCGCGCGTTCGTCGTGAGCGCATGGGCCATATTGAGCTCGCAGCACCTGTTGCCCATATCTgg                                             >  2:4650543/1‑150 (MQ=255)
                                                                                                    aaGGATTATGAATGCTTGTGCGGCAAGTACAAGCGTATGAAATACAAAGGCATCATCTGCGAAAAGTGCGGCGTTGAAGTCACGCTGTCGCGCGTTCGTCGTGAGCGCATGGGCCATATTGAGCTCGCAGCACCTGTTGCCCATATCTgg                                             >  1:4749015/1‑150 (MQ=255)
                                                                                                    aaGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTgg                                             >  2:4715108/1‑150 (MQ=255)
                                                                                                    aaGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTgg                                             >  1:1449361/1‑150 (MQ=255)
                                                                                                    aaGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTgg                                             >  1:2190457/1‑150 (MQ=255)
                                                                                                     aGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGt                                            >  2:2747295/1‑150 (MQ=255)
                                                                                                       gACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTc                                          >  2:3149886/1‑150 (MQ=255)
                                                                                                       gACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTc                                          >  2:3452821/1‑150 (MQ=255)
                                                                                                          tACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTg                                       <  1:2987023/150‑1 (MQ=255)
                                                                                                          tACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTg                                       >  1:3467467/1‑150 (MQ=255)
                                                                                                          tACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTg                                       >  1:2341963/1‑150 (MQ=255)
                                                                                                          tACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTg                                       >  2:2134638/1‑150 (MQ=255)
                                                                                                           aCGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGa                                      <  2:2439120/150‑1 (MQ=255)
                                                                                                            cGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGaa                                     >  2:1691273/1‑150 (MQ=255)
                                                                                                             gAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAg                                    >  1:4448810/1‑150 (MQ=255)
                                                                                                             gAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAg                                    >  1:4406626/1‑150 (MQ=255)
                                                                                                              aGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAGt                                   >  2:4988533/1‑150 (MQ=255)
                                                                                                                  ccTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAGTCGCt                               <  1:1570223/150‑1 (MQ=255)
                                                                                                                    tgtgCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCACATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAGTCGCTGc                             >  1:1518651/1‑150 (MQ=255)
                                                                                                                     gtgCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAGTCGCTGcc                            >  1:2470417/1‑150 (MQ=255)
                                                                                                                          gCAAGTACAAGCGTATGAAATACAAAGGCATCATCTGCGAAAAGTGCGGCGTTGAAGTCACGCTGTCGCGCGTTCGTCGTGAGCGCATGGGCCATATTGAGCTCGCAGCACCTGTTGCCCATATCTGGTTCTTGAAGTCGCTGCCATcgc                       <  1:5223/150‑3 (MQ=255)
                                                                                                                          gCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCAt                                                                                     <  2:2879627/88‑1 (MQ=255)
                                                                                                                          gCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCAt                                                                                     >  1:2879627/1‑88 (MQ=255)
                                                                                                                          gCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAGTCGCTGCCGTccc                       <  2:798312/150‑1 (MQ=255)
                                                                                                                               tACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAGTCGCTGCCGTCCCGTATc                  >  2:3918444/1‑150 (MQ=255)
                                                                                                                               tACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAGTCGCTGCCGTCCCGTATc                  >  2:2219174/1‑150 (MQ=255)
                                                                                                                                  aaGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAGTCGCTGCCGTCCCGTATCGGc               >  1:3677645/1‑150 (MQ=255)
                                                                                                                                  aaGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAGTCGCTGCCGTCCCGTATCGGc               >  2:4099049/1‑150 (MQ=255)
                                                                                                                                  aaGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAGTCGCTGCCGTCCCGTATCGGc               >  1:2283611/1‑150 (MQ=255)
                                                                                                                                  aaGCGACTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAGTCGCTGCCGTCCCGTATCGGc               >  2:1944307/1‑150 (MQ=255)
                                                                                                                                      gcCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAGTCGCTGCCGTCCCGTATCGGCTTGc           >  1:1639655/1‑150 (MQ=255)
                                                                                                                                           aaGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAGTCGCTGCCGTCCCGTATCGGCTTGCTGATg      <  1:3350308/150‑1 (MQ=255)
                                                                                                                                            aGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAGTCGCTGCCGTCCCGTATCGGCTTGCTGATgg     <  1:3061594/150‑1 (MQ=255)
                                                                                                                                             gCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAGTCGCTGCCGTCCCGTATCGGCTTGCTGATGGa    >  2:3313412/1‑150 (MQ=255)
                                                                                                                                              cACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGATCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAGTCGCTGCCGTCCCGTATCGGCTTGCTGATGGAc   <  1:4586794/150‑1 (MQ=255)
                                                                                                                                               aCCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAGTCGCTGCCGTCCCGTATCGGCTTGCTGATGGACa  >  2:4938237/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GTGGTCGTTCGGTGAAGTTAAGAAGCCGGAAACCATCAACTACCGTACGTTCAAGCCTGAGCGTGACGGCCTGTTCTGCGCCAAGATCTTTGGCCCAGTCAAGGACTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTCAAGCACCGCGGCGTAATCTGCGAGAAGTGCGGCGTTGAAGTTGCCCTGGCGAAGGTTCGTCGTGAGCGCATGGCGCACATCGAGCTGGCCTCGCCGGTTGCCCACATCTGGTTCCTGAAGTCGCTGCCGTCCCGTATCGGCTTGCTGATGGACA  >  NC_002947/541859‑542151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑