Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 543,059 G→T 9.1% L432L (CTG→CTT)  rpoC → DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947543,0590GT 9.1% 459.7 / 25.1 143L432L (CTG→CTT) rpoCDNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base G (62/68);  new base T (6/7);  total (68/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 149 total aligned reads displayed.

GCAAGCTGGAAATGCGTGGTCTGGCGACCACCATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTGACCCTGGAAGCCCAGCTCGAAGCGCGTGC  >  NC_002947/542911‑543208
                                                                                                                                                    |                                                                                                                                                     
gCAAGCTGGAAATGCGTGGTCTGGCGACCACCATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTgg                                                                                                                                                      <  2:1975729/150‑1 (MQ=255)
gCAAGCTGGAAATGCGTGGTCTGGCGACCACCATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTgg                                                                                                                                                      <  1:3141913/150‑1 (MQ=255)
 cAAGCTGGAAATGCGTGGTCTGGCGACCACCATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCGCCGTCTggg                                                                                                                                                     >  1:4816807/1‑150 (MQ=255)
            tGCGTGGTCTGGCGACCACCATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGt                                                                                                                                          <  1:5055824/150‑1 (MQ=255)
            tGCGTGGTCTGGCGACCACCATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGt                                                                                                                                          <  1:5055836/150‑1 (MQ=255)
             gCGTGGTCTGGCGACCACCATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGtt                                                                                                                                         <  1:314637/150‑1 (MQ=255)
              cGTGGTCTGGCGACCACCATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGttt                                                                                                                                        <  2:4890263/150‑1 (MQ=255)
              cGTGGTCTGGCGACCACCATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGttt                                                                                                                                        <  2:1032122/150‑1 (MQ=255)
              cGTGGTCTGGCGACCACCATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGttt                                                                                                                                        <  2:4030791/150‑1 (MQ=255)
                 ggTCTGGCGACCACCATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGaa                                                                                                                                     <  1:1077740/150‑1 (MQ=255)
                  gTCTGGCGACCACCATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAAc                                                                                                                                    <  2:3361066/150‑1 (MQ=255)
                  gTCTGGCGACCACCATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAAc                                                                                                                                    <  2:138189/150‑1 (MQ=255)
                  gTCTGGCGACCACCATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAAc                                                                                                                                    <  2:137925/150‑1 (MQ=255)
                  gTCTGGCGACCACCATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAAc                                                                                                                                    <  2:4833397/150‑1 (MQ=255)
                     tGGCGACCACCATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCg                                                                                                                                 <  2:411514/150‑1 (MQ=255)
                     tGGCGACCACCATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCg                                                                                                                                 >  2:715388/1‑150 (MQ=255)
                       gCGACCACCATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTa                                                                                                                               >  2:3392671/1‑150 (MQ=255)
                          accaccATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTg                                                                                                                            <  2:4791326/150‑1 (MQ=255)
                               caTCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGa                                                                                                                       >  1:82345/1‑150 (MQ=255)
                                aTCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGaa                                                                                                                      <  1:2869300/150‑1 (MQ=255)
                                aTCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGaa                                                                                                                      <  1:2869256/150‑1 (MQ=255)
                                 tCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGTTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAg                                                                                                                     <  1:619761/150‑1 (MQ=255)
                                 tCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAg                                                                                                                     >  1:2994665/1‑150 (MQ=255)
                                 tCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAg                                                                                                                     <  1:3653476/150‑1 (MQ=255)
                                 tCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAg                                                                                                                     >  2:585077/1‑150 (MQ=255)
                                 tCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAg                                                                                                                     >  2:585078/1‑150 (MQ=255)
                                   aaGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGt                                                                                                                   <  2:3236572/150‑1 (MQ=255)
                                      gCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAg                                                                                                                >  1:4426338/1‑150 (MQ=255)
                                       cTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAgg                                                                                                               <  2:4428766/150‑1 (MQ=255)
                                       cTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAgg                                                                                                               <  1:1045106/150‑1 (MQ=255)
                                       cTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAgg                                                                                                               <  2:277986/150‑1 (MQ=255)
                                            aagaagATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTgg                                                                                                                                                      >  1:2802097/1‑106 (MQ=255)
                                                agaTGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGc                                                                                                      <  2:1002889/150‑1 (MQ=255)
                                                 gaTGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGGTTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCt                                                                                                     <  1:2983167/150‑1 (MQ=255)
                                                 gaTGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCt                                                                                                     <  2:3757614/150‑1 (MQ=255)
                                                 gaTGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCt                                                                                                     <  1:2047271/150‑1 (MQ=255)
                                                           cgcgAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCtt                                                                                           <  1:706046/150‑2 (MQ=255)
                                                             cgAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGt                                                                                         >  1:3393157/1‑150 (MQ=255)
                                                                    ccAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGtgct                                                                                  >  2:1627235/1‑150 (MQ=255)
                                                                     cAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTAT‑CAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGtgctgc                                                                                <  2:3075987/150‑1 (MQ=255)
                                                                      agagGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGtgctgc                                                                                >  2:3922006/1‑150 (MQ=255)
                                                                            gtgGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACaa                                                                          <  1:397961/150‑1 (MQ=255)
                                                                              gggACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACg                                                                        >  2:3663637/1‑150 (MQ=255)
                                                                              gggACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACg                                                                        >  2:1445271/1‑150 (MQ=255)
                                                                               ggACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGc                                                                       >  1:4817445/1‑150 (MQ=255)
                                                                                 aCGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCg                                                                     <  1:3895522/150‑1 (MQ=255)
                                                                                   gTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGAc                                                                   <  1:45005/150‑1 (MQ=255)
                                                                                   gTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGAc                                                                   >  1:4374418/1‑150 (MQ=255)
                                                                                     tctcGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACtt                                                                 <  1:1439164/150‑1 (MQ=255)
                                                                                       tcGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCg                                                               >  1:2216256/1‑150 (MQ=255)
                                                                                        cGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGa                                                              >  2:4030775/1‑150 (MQ=255)
                                                                                         gCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGAc                                                             >  2:1440/1‑150 (MQ=255)
                                                                                         gCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGAc                                                             >  2:4139624/1‑150 (MQ=255)
                                                                                         gCTGAAGAGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGCAGGTAAGGCTATTCAGCTGCCCCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGAc                                                             >  2:3401393/1‑150 (MQ=255)
                                                                                               gTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGAc                                                       >  2:2097508/1‑150 (MQ=255)
                                                                                                tGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGAcc                                                      <  1:645981/150‑1 (MQ=255)
                                                                                                tGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGAcc                                                      <  2:4611230/150‑1 (MQ=255)
                                                                                                 gATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCa                                                     <  1:4544931/150‑1 (MQ=255)
                                                                                                 gATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCa                                                     >  1:373442/1‑150 (MQ=255)
                                                                                                   ttCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGa                                                   >  2:3854907/1‑150 (MQ=255)
                                                                                                            acCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTc                                          <  2:5058851/150‑1 (MQ=255)
                                                                                                              cccGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCAc                                        >  2:3510644/1‑150 (MQ=255)
                                                                                                              cccGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCAc                                        >  2:514686/1‑150 (MQ=255)
                                                                                                              cccGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCAc                                        <  2:2928232/150‑1 (MQ=255)
                                                                                                              cccGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATGCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCAc                                        >  1:4714279/1‑150 (MQ=255)
                                                                                                                  tACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGc                                    <  2:256562/150‑1 (MQ=255)
                                                                                                                     tGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGc                                 >  2:2167824/1‑150 (MQ=255)
                                                                                                                     tGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGc                                 >  1:1645533/1‑150 (MQ=255)
                                                                                                                      tctgaaCCGTGCGCCTACGCTGCACCGTCTTGGTATTCAGGCATTCGAACCCACGCTGATCGAAGGCAAGGCCATTCAGCTGCATCCGCTCGTCTGTACAGCGTTCAACGCTGACTTCGACGGTGACCAGATGGCTGTTCACGTTCctct                                >  1:2615247/5‑150 (MQ=255)
                                                                                                                       cTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTg                               >  2:113914/1‑150 (MQ=255)
                                                                                                                        tgaaCCGTGCGCCTACGCTGCACCGTCTTGGTATTCAGGCATTCGAACCCACGCTGATCGAAGGCAAGGCCATTCAGCTGCATCCGCTCGTCTGTACAGCGTTCAACGCTGACTTCGACGGTGACCAGATGGCTGTTCACGTTCCTCTgt                              >  2:1155098/3‑149 (MQ=255)
                                                                                                                        tCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGATCCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTGa                              <  2:4216121/150‑1 (MQ=255)
                                                                                                                        tCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTGa                              >  1:577006/1‑150 (MQ=255)
                                                                                                                        tCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTGa                              >  2:2397130/1‑150 (MQ=255)
                                                                                                                        tCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTGa                              <  2:4216135/150‑1 (MQ=255)
                                                                                                                          aaCCGTGCACAGACCCTTCACAGTCTGGGTATCCAGGCGTTTGAACCAGTACTGATCGAAGGTAAGGATATTCAGCTGCACACGCTGGTCTGTGATGAGTACAACGCCGACTTCGACGGTGACAAGATGTCCGTTCACTTGCAGCTGAca                            >  2:2888204/1‑149 (MQ=255)
                                                                                                                             cGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTGACCCTg                         <  2:3335488/150‑1 (MQ=255)
                                                                                                                             cGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTGACCCTg                         <  2:2256817/150‑1 (MQ=255)
                                                                                                                             cGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTGACCCTg                         <  1:2227588/150‑1 (MQ=255)
                                                                                                                              gTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCt                                                                                       <  2:2354767/87‑1 (MQ=255)
                                                                                                                              gTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCt                                                                                       >  1:2354767/1‑87 (MQ=255)
                                                                                                                                gCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTGACCCTGGaa                      <  2:2209115/150‑1 (MQ=255)
                                                                                                                                    cGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTGACCCTGGAAGccc                  >  1:4899210/1‑150 (MQ=255)
                                                                                                                                     tacgctgcACCGTCTTGGTATTCAGGCATTCGAACGCACGCTGATCGAAGGCTAGGCCATTCAGCTGCATCCGCTCGTCTGTACAGCGTTCAACGCTGACTTCGACGGTGACCAGATGGCTGTTCACGTTCCTCTGTCGCTTGAAGCTCa                 <  2:146063/143‑1 (MQ=255)
                                                                                                                                      aCCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTGACCCTGGAAGCCCAg                <  2:2878118/150‑1 (MQ=255)
                                                                                                                                       cgctgcACCGTCTTGGTATTCAGGCATTCGAACCCACGCTGATCGAAGGCAAGGCCATTCAGCTGCATCCGCTCGTCTGTACAGCGTTCAACGCTGACTTCGACGGTGACCAGATGGCTGTTCACGTTCCTCTGTCGCTTGAAGCTCAGc               <  1:1270363/145‑1 (MQ=255)
                                                                                                                                        gctgcACCGTCTTGGTATTCAGGCATTCGAACCCACGCTGATCGAAGGCAAGGCCATTCAGCTGCATCCGCTCGTCTGTACAGCGTTCAACGCTGACTTCGACGGTGACCAGATGGCTGTTCACGTTCCTCTGTCGCTTGAAGCTCAGCt              <  1:4708438/146‑1 (MQ=255)
                                                                                                                                        gctgcACCGTCTTGGTATTCAGGCATTCGAACCCACGCTGATCGAAGGCAAGGCCATTCAGCTGCATCCGCTCGTCTGTACAGCGTTCAACGCTGACTTCGACGGTGACCAGATGGCTGTTCACGTTCCTCTGTCGCTTGAAGCTCAGCt              <  2:2500194/146‑1 (MQ=255)
                                                                                                                                        gctgcACCGTCTTGGTATTCAGGCATTCGAACCCACGCTGATCGAAGGCAAGGCCATTCAGCTGCATCCGCTCGTCTGTACAGCGTTCAACGCTGACTTCGACGGTGACCAGATGGCTGTTCACGTTCCTCTGTCGCTTGAAGCTCAGCt              >  2:2949635/5‑150 (MQ=255)
                                                                                                                                        ccTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTGACCCTGGAAGCCCAGCt              >  1:3433725/1‑150 (MQ=255)
                                                                                                                                          ttCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAATGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTGACCCTGGAAGCCCAGCTCg            >  2:4342544/1‑150 (MQ=255)
                                                                                                                                           gcacaGTCTTGGTATTCAGGCATTCGAACCCACGCTGATCGAAGGCAAGGCCATTCAGCTGCATCCGCTCGTCTGTACAGCGTTCAACGCTGACTTCGACGGTGACCAGATGGCTGTTCACGTTCATCTGTCGCTTGAAGCTCAGCTTGa           >  2:2642241/2‑150 (MQ=255)
                                                                                                                                            cACCGTCTTGGTATTCAGGCATTCGAACCCACGCTGATCGAAGGCAAGGCCATTCAGCTGCATCCGCTCGTCTGTACAGCGTTCAACGCTGACTTCGACGGTGACCAGATGGCTGTTCACGTTCCTCTGTCGCTTGAAGCTCAGCTTGaa          <  1:4858093/150‑1 (MQ=255)
                                                                                                                                             aCCGTCTTGGTATTCAGGCATTCGAACCCACGCTGATCGAAGGCAAGGCCATTCAGCTGCATCCGCTCGTCTGTACAGCGTTCAACGCTGAGTTCGACGGTGACCAGATGGCTGTTCACGTTCCTCTGTCGCTTGAAGCTCTGCTTGAAg         >  2:1764194/1‑150 (MQ=255)
                                                                                                                                                gTCTTGGTATTCAGGCATTCGAACCCACGCTGATCGAAGGCAAGGCCATTCAGCTGCATCCGCTCGTCTGTACAGCGTTCAACGCTGACTTCGACGGTGACCAGATGGCTGTTCACGTTCCTCTGTCGCTTGAAGCTCAGCTTGAAgcac      <  1:283836/150‑3 (MQ=255)
                                                                                                                                                  cTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTGACCCTGGAAGCCCAGCTCGAAGCGCGt    <  1:2060776/150‑1 (MQ=255)
                                                                                                                                                  cTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTGACCCTGGAAGCCCAGCTCGAAGCGCGt    <  1:715388/150‑1 (MQ=255)
                                                                                                                                                   tGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTGACCCTGGAAGCCCAGCTCGAAGCGCGTg   >  2:3077956/1‑150 (MQ=255)
                                                                                                                                                   tGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGATGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTGACCCTGGAAGCCCAGCTCGAAGCGCGTg   >  2:213840/1‑150 (MQ=255)
                                                                                                                                                    gggTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTGACCCTGGAAGCCCAGCTCGAAGCGCGTgc  >  2:741244/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCAAGCTGGAAATGCGTGGTCTGGCGACCACCATCAAGGCTGCCAAGAAGATGGTCGAGCGCGAGCTGCCAGAGGTGTGGGACGTTCTCGCTGAAGTGATTCGCGAACACCCCGTACTGCTCAACCGTGCACCGACCCTTCACCGTCTGGGTATCCAGGCGTTTGAACCCGTACTGATCGAAGGTAAGGCTATTCAGCTGCACCCGCTGGTCTGTGCTGCGTACAACGCCGACTTCGACGGTGACCAGATGGCCGTTCACGTGCCGCTGACCCTGGAAGCCCAGCTCGAAGCGCGTGC  >  NC_002947/542911‑543208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑