BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	547,444	T→C	6.0%	I97I (ATT→ATC)	fusA →	elongation factor G 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	547,444	0	T	C	6.0%	468.6 / 12.4	149	I97I (ATT→ATC)	fusA	elongation factor G 1
Reads supporting (aligned to +/- strand): ref base T (74/66); new base C (7/2); total (81/68)
Fisher's exact test for biased strand distribution p-value = 1.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

Only 100 of 151 total aligned reads displayed.

GCGACCACCGACTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATGGACCGTG  >  NC_002947/547295‑547592

gcgACCACCGACTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCAtt                                                                                                                                                      <  2:4875983/150‑1 (MQ=255)

   accaccGACTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGaa                                                                                                                                                   <  2:2420185/150‑1 (MQ=255)

      accGACTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTa                                                                                                                                                >  2:5122327/1‑150 (MQ=255)

      accGACTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTa                                                                                                                                                >  2:3915289/1‑150 (MQ=255)

      accGACTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTa                                                                                                                                                <  2:2711148/150‑1 (MQ=255)

       ccGACTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGGAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTag                                                                                                                                               >  2:438066/1‑150 (MQ=255)

           cTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCg                                                                                                                                           <  1:1965745/150‑1 (MQ=255)

             ggATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGtt                                                                                                                                         <  2:2778226/150‑1 (MQ=255)

                tGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGc                                                                                                                                      >  2:1446459/1‑150 (MQ=255)

                   tGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGc                                                                                                                                   <  1:4675197/150‑1 (MQ=255)

                      aggagcaggagcGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCgtg                                                                                                                                >  2:942190/1‑150 (MQ=255)

                      aggagcaggagcGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCgtg                                                                                                                                >  1:2540155/1‑150 (MQ=255)

                        gagcaggagcGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTa                                                                                                                              <  1:1541663/150‑1 (MQ=255)

                        gagcaggagcGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTa                                                                                                                              >  1:640639/1‑150 (MQ=255)

                        gagcaggagcGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGGGTa                                                                                                                              >  2:5015397/1‑150 (MQ=255)

                              gagcGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGAc                                                                                                                        >  2:4047702/1‑150 (MQ=255)

                                       aTCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTc                                                                                                               >  1:5066256/1‑150 (MQ=255)

                                          aCCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGtt                                                                                                            <  1:3100294/150‑1 (MQ=255)

                                          aCCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGtt                                                                                                            <  1:3100339/150‑1 (MQ=255)

                                          aCCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGtt                                                                                                            <  1:471982/150‑1 (MQ=255)

                                            cATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTtgt                                                                                                          <  2:3356601/150‑1 (MQ=255)

                                            cATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTtgt                                                                                                          <  1:2941979/150‑1 (MQ=255)

                                            cATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTtgt                                                                                                          >  1:4056594/1‑150 (MQ=255)

                                             atgaCCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTtgtg                                                                                                         <  2:2970800/147‑1 (MQ=255)

                                               tACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGtt                                                                                                       <  1:3942230/150‑1 (MQ=255)

                                                aCCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTc                                                                                                      >  2:1725621/1‑150 (MQ=255)

                                                 ccTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCt                                                                                                     >  2:4874458/1‑150 (MQ=255)

                                                 ccTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCt                                                                                                     >  2:4403668/1‑150 (MQ=255)

                                                          ccGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCt                                                                                            >  1:5037488/1‑150 (MQ=255)

                                                           cGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTc                                                                                           >  2:4860469/1‑150 (MQ=255)

                                                            gTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTcc                                                                                          >  2:1932499/1‑150 (MQ=255)

                                                               accaccTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGc                                                                                       <  2:3367801/150‑1 (MQ=255)

                                                                 caccTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGt                                                                                     >  2:4177512/1‑150 (MQ=255)

                                                                  accTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGtt                                                                                    <  2:749848/150‑1 (MQ=255)

                                                                        tGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCg                                                                              <  1:1816295/150‑1 (MQ=255)

                                                                          gAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCa                                                                            <  1:4134340/150‑1 (MQ=255)

                                                                           aaaGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAg                                                                           >  2:5060118/1‑150 (MQ=255)

                                                                             aGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTc                                                                         <  2:3275836/150‑1 (MQ=255)

                                                                                  cccGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGaaa                                                                    <  2:829213/150‑1 (MQ=255)

                                                                                       ggTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTa                                                               <  2:3705942/150‑1 (MQ=255)

                                                                                        gTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTAt                                                              <  2:3281216/150‑1 (MQ=255)

                                                                                        gTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTAt                                                              <  2:2498090/150‑1 (MQ=255)

                                                                                         tCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATg                                                             >  2:4116504/1‑150 (MQ=255)

                                                                                           aGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGc                                                           <  1:1701488/150‑1 (MQ=255)

                                                                                               tGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCa                                                       >  1:3223364/1‑150 (MQ=255)

                                                                                               tGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCa                                                       >  1:2746602/1‑150 (MQ=255)

                                                                                               tGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTATGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCa                                                       >  1:621833/1‑150 (MQ=255)

                                                                                                gACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAg                                                      <  2:4376574/150‑1 (MQ=255)

                                                                                                gACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAg                                                      >  1:412620/1‑150 (MQ=255)

                                                                                                   aaCTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCgatttgtg                                                   <  2:3218196/150‑8 (MQ=255)

                                                                                                   aaCTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCgatttgtg                                                   <  2:3218159/150‑8 (MQ=255)

                                                                                                   aaCTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCgatttgtg                                                   <  2:1809374/150‑8 (MQ=255)

                                                                                                   aaCTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCgatttgtg                                                   <  2:1809541/150‑8 (MQ=255)

                                                                                                   aaCTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGcc                                                   >  2:2142567/1‑150 (MQ=255)

                                                                                                        ccGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCcaacaa                                              >  1:427994/1‑150 (MQ=255)

                                                                                                        ccGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCcaacaa                                              <  1:1030213/150‑1 (MQ=255)

                                                                                                            gTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTAc                                          >  1:2128537/1‑150 (MQ=255)

                                                                                                             tAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACg                                         <  2:2360344/150‑1 (MQ=255)

                                                                                                             tAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACg                                         <  2:4168613/150‑1 (MQ=255)

                                                                                                                aCGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCg                                      >  1:2053757/1‑150 (MQ=255)

                                                                                                                aCGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCg                                      >  1:4399527/1‑150 (MQ=255)

                                                                                                                  gTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGtt                                    >  2:4091538/1‑150 (MQ=255)

                                                                                                                  gTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGtt                                    >  2:4769506/1‑150 (MQ=255)

                                                                                                                  gTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGtt                                    >  2:2604976/1‑150 (MQ=255)

                                                                                                                   ttATCTATACCCCGGGCAACGTTGTCTTCACATTTGTATTAGAGACTTGGCTGCTTGTTCTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTc                                   <  1:2382572/150‑1 (MQ=255)

                                                                                                                   ttATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTc                                   >  2:297366/1‑150 (MQ=255)

                                                                                                                    tATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTcc                                  >  1:1224963/1‑150 (MQ=255)

                                                                                                                     aTCGATTCCCCTGGCCAAGTTGACTTCACCATTGTAGTAGAGCGTTCGCTGCGTGTACTCGAGGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGAGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTcct                                 <  2:1089663/150‑2 (MQ=255)

                                                                                                                     aTCGACACCCCGGGCCACGTTGACTTCACCATCGAAGTCGAGCGTTCGCTCCGCGTTCTCGACGGTGCAATCGCTCTTCTCGATGCGAATGCTGGCGTAGAGCCACAGACCGAAACTGTTTGGCGTCAGGCTGAGAAGTACCACGTTcca                                 >  2:798661/1‑149 (MQ=255)

                                                                                                                     aTCGACACCCCGGGCCACGTTGACTTCACCATCGAAGTCGAGCGTTCGCTCCGCGTTCTCGACGGTGCAATCGCTCTTCTCGATGCGAATGCTGGCGTAGAGCCACAGACCGAAACTGTTTGGCGTCAGGCTGAGAAGTACCACGTTcca                                 >  2:1593985/1‑149 (MQ=255)

                                                                                                                        gATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCgt                              <  2:2444836/150‑1 (MQ=255)

                                                                                                                         acaCCCCGGGCCACGTTGACTTCACCATCGAAGTCGAGCGTTCGCTCCGCGTTCTCGACGGTGCAATCGCTCTTCTCGATGCGAATGCTGGCGTAGAGCCACAGACCGAAACTGTTTGGCGTCAGGCTGAGAAGTACCACGTTCCACgta                             >  1:3717183/3‑149 (MQ=255)

                                                                                                                         acaCCCCGGGCCACGTTGACTTCACCATCGAAGTCGAGCGTTCGCTCCGCGTTCTCGACGGTGCAATCGCTCTTCTCGATGCGAATGCTGGCGTAGAGCCACAGACCGAAACTGTTTGGCGTCAGGCTGAGAAGTACCACGTTCCACgta                             >  1:4783331/3‑149 (MQ=255)

                                                                                                                         acaCCCCGGGCCACGTTGACTTCACCATCGAAGTCGAGCGTTCGCTCCGCGTTCTCGACGGTGCAATCGCTCTTCTCGATGCGAATGCTGGCGTAGAGCCACAGACCGAAACTGTTTGGCGTCAGGCTGAGAAGTACCACGTTCCACgta                             >  1:4783236/3‑149 (MQ=255)

                                                                                                                          tACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCACCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCgtgt                            >  1:4322088/1‑150 (MQ=255)

                                                                                                                            ccccTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGtgttg                          <  2:473331/150‑1 (MQ=255)

                                                                                                                             cccTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGtgttgt                         <  1:3599679/150‑1 (MQ=255)

                                                                                                                             cccGGGCCACGTTGACTTCACCATCGAAGTCGAGCGTTCGCTCCGCGTTCTCGACGGTGCAATCGCTCTTCTCGATGCGAATGCTGGCGTAGAGCCACAGACCGAAACTGTTTGGCGTCAGGCTGAGAAGTACCACGTTCCACgtatggt                         >  2:1039585/1‑145 (MQ=255)

                                                                                                                             cccGGGCCACGTTGACTTCACCATCGAAGTCGAGCGTTCGCTCCGCGTTCTCGACGGTGCAATCGCTCTTCTCGATGCGAATGCTGGCGTAGAGCCACAGACCGAAACTGTTTGGCGTCAGGCTGAGAAGTACCACGTTCCACgtatggt                         >  1:3342779/1‑145 (MQ=255)

                                                                                                                                tGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTa                      <  2:1969/150‑1 (MQ=255)

                                                                                                                                tGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTa                      <  2:2077/150‑1 (MQ=255)

                                                                                                                                 ggCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTAc                     <  2:4797031/150‑1 (MQ=255)

                                                                                                                                 ggCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTAc                     >  1:71279/1‑150 (MQ=255)

                                                                                                                                   ccACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGt                   <  1:2377591/150‑1 (MQ=255)

                                                                                                                                   ccACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGt                   <  1:438066/150‑1 (MQ=255)

                                                                                                                                    cACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTg                  >  2:1622076/1‑150 (MQ=255)

                                                                                                                                       gTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCa                                                       >  1:2484462/1‑110 (MQ=255)

                                                                                                                                       gTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAAc               >  1:1500138/1‑150 (MQ=255)

                                                                                                                                       gTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAAc               <  1:3915289/150‑1 (MQ=255)

                                                                                                                                        ttGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACa              >  1:79395/1‑150 (MQ=255)

                                                                                                                                         tGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACaa             <  2:2716094/150‑1 (MQ=255)

                                                                                                                                          gACATCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAg            <  2:2758907/150‑1 (MQ=255)

                                                                                                                                             ttCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATg         >  2:3677173/1‑150 (MQ=255)

                                                                                                                                             ttCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATg         >  1:1216578/1‑150 (MQ=255)

                                                                                                                                              tCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGtgtt                           <  1:1974179/131‑1 (MQ=255)

                                                                                                                                                aCCATTGAAGTAGAGCGTTCGCTGCGGGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTTAGCCGCAGTCAGAAACCGTATGGCGTCAGGCAAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATTGAc      >  2:1354450/1‑150 (MQ=255)

                                                                                                                                                 ccATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCCTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATGGAcc     >  1:1004054/1‑150 (MQ=255)

                                                                                                                                                  cATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCCGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATGGACCg    >  1:3996248/1‑150 (MQ=255)

                                                                                                                                                   aTTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATGGACCGt   >  2:2402890/1‑150 (MQ=255)

                                                                                                                                                    ttGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATGGACCGTg  >  2:4649110/1‑150 (MQ=255)

GCGACCACCGACTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATGGACCGTG  >  NC_002947/547295‑547592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`