BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	547,450	A→C	6.2%	V99V (GTA→GTC)	fusA →	elongation factor G 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	547,450	0	A	C	6.2%	493.5 / 16.3	146	V99V (GTA→GTC)	fusA	elongation factor G 1
Reads supporting (aligned to +/- strand): ref base A (74/63); new base C (7/2); total (81/65)
Fisher's exact test for biased strand distribution p-value = 2.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 152 total aligned reads displayed.

ACCGACTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATGGACCGTGCTGGTG  >  NC_002947/547301‑547598

accGACTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTa                                                                                                                                                      <  2:2711148/150‑1 (MQ=255)

accGACTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTa                                                                                                                                                      >  2:5122327/1‑150 (MQ=255)

 ccGACTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGGAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTag                                                                                                                                                     >  2:438066/1‑150 (MQ=255)

  cGACTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTaga                                                                                                                                                    <  1:98457/150‑1 (MQ=255)

     cTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCg                                                                                                                                                 <  1:1965745/150‑1 (MQ=255)

          tGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGc                                                                                                                                            >  2:1446459/1‑150 (MQ=255)

             tGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGc                                                                                                                                         <  1:4675197/150‑1 (MQ=255)

             tGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGc                                                                                                                                         <  1:3501554/150‑1 (MQ=255)

               caggagcaggagCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCgt                                                                                                                                       >  1:3168200/1‑150 (MQ=255)

                aggagcaggagcGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCgtg                                                                                                                                      >  2:942190/1‑150 (MQ=255)

                aggagcaggagcGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCgtg                                                                                                                                      >  1:2540155/1‑150 (MQ=255)

                  gagcaggagcGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTa                                                                                                                                    >  1:640639/1‑150 (MQ=255)

                  gagcaggagcGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTa                                                                                                                                    <  1:1541663/150‑1 (MQ=255)

                  gagcaggagcGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGGGTa                                                                                                                                    >  2:5015397/1‑150 (MQ=255)

                        gagcGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGAc                                                                                                                              >  2:4047702/1‑150 (MQ=255)

                                 aTCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTc                                                                                                                     >  1:5066256/1‑150 (MQ=255)

                                    aCCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGtt                                                                                                                  <  1:471982/150‑1 (MQ=255)

                                    aCCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGtt                                                                                                                  <  1:3100294/150‑1 (MQ=255)

                                     ccATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTtg                                                                                                                 <  2:1203638/150‑1 (MQ=255)

                                      cATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTtgt                                                                                                                <  1:2941979/150‑1 (MQ=255)

                                      cATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTtgt                                                                                                                >  2:2416943/1‑150 (MQ=255)

                                      cATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTtgt                                                                                                                >  1:4056594/1‑150 (MQ=255)

                                      cATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTtgt                                                                                                                >  1:4056631/1‑150 (MQ=255)

                                       atgaCCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTtgtg                                                                                                               <  2:2970800/147‑1 (MQ=255)

                                         tACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGtt                                                                                                             <  1:3942230/150‑1 (MQ=255)

                                          aCCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTc                                                                                                            >  2:1725621/1‑150 (MQ=255)

                                           ccTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCt                                                                                                           >  2:4403578/1‑150 (MQ=255)

                                           ccTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCt                                                                                                           >  2:4874458/1‑150 (MQ=255)

                                             tCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCtgt                                                                                                         >  2:1897816/1‑150 (MQ=255)

                                                 cTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCa                                                                                                     >  2:416252/1‑150 (MQ=255)

                                                  tGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCAc                                                                                                    <  1:4184692/150‑1 (MQ=255)

                                                     cGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTc                                                                                                 >  2:4860469/1‑150 (MQ=255)

                                                     cGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGCGGCACCTc                                                                                                 >  2:2653932/1‑150 (MQ=255)

                                                         accaccTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGc                                                                                             <  2:3367801/150‑1 (MQ=255)

                                                          ccaccTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCg                                                                                            >  2:2859544/1‑150 (MQ=255)

                                                           caccTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGt                                                                                           >  2:4177512/1‑150 (MQ=255)

                                                            accTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGtt                                                                                          <  2:749848/150‑1 (MQ=255)

                                                                 cTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGcc                                                                                     <  2:1757223/150‑1 (MQ=255)

                                                                  tGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCg                                                                                    <  1:1816295/150‑1 (MQ=255)

                                                                    gAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCa                                                                                  <  2:2294101/150‑1 (MQ=255)

                                                                    gAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCa                                                                                  <  1:4134340/150‑1 (MQ=255)

                                                                     aaaGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAg                                                                                 >  2:5060118/1‑150 (MQ=255)

                                                                       aGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTc                                                                               <  2:3275836/150‑1 (MQ=255)

                                                                           tCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGaa                                                                           >  1:3813010/1‑150 (MQ=255)

                                                                            cccGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGaaa                                                                          <  2:829213/150‑1 (MQ=255)

                                                                            cccGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGaaa                                                                          <  2:4071769/150‑1 (MQ=255)

                                                                                 ggTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTa                                                                     <  2:3705942/150‑1 (MQ=255)

                                                                                  gTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTAt                                                                    <  2:2498090/150‑1 (MQ=255)

                                                                                   tCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATg                                                                   >  2:4116504/1‑150 (MQ=255)

                                                                                     aGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGc                                                                 <  1:1701488/150‑1 (MQ=255)

                                                                                         tGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCa                                                             >  1:3223364/1‑150 (MQ=255)

                                                                                         tGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCa                                                             >  1:2746602/1‑150 (MQ=255)

                                                                                         tGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCa                                                             >  1:658342/1‑150 (MQ=255)

                                                                                             aaCTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCgatttgtg                                                         <  2:3218159/150‑8 (MQ=255)

                                                                                             aaCTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCgatttgtg                                                         <  2:1809541/150‑8 (MQ=255)

                                                                                             aaCTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGcc                                                         >  2:2142567/1‑150 (MQ=255)

                                                                                                  ccGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCcaacaa                                                    >  2:4709681/1‑150 (MQ=255)

                                                                                                  ccGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCcaacaa                                                    <  1:1030213/150‑1 (MQ=255)

                                                                                                  ccGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCcaacaa                                                    >  1:427994/1‑150 (MQ=255)

                                                                                                      gTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTAc                                                >  1:2128537/1‑150 (MQ=255)

                                                                                                          aCGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCg                                            >  1:2053757/1‑150 (MQ=255)

                                                                                                          aCGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCg                                            >  1:4399527/1‑150 (MQ=255)

                                                                                                            gTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGtt                                          >  2:4769624/1‑150 (MQ=255)

                                                                                                            gTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGtt                                          >  2:4769506/1‑150 (MQ=255)

                                                                                                             ttATCTATACCCCGGGCAACGTTGTCTTCACATTTGTATTAGAGACTTGGCTGCTTGTTCTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTc                                         <  1:2382572/150‑1 (MQ=255)

                                                                                                              tATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTcc                                        >  1:1224963/1‑150 (MQ=255)

                                                                                                               aTCGATTCCCCTGGCCAAGTTGACTTCACCATTGTAGTAGAGCGTTCGCTGCGTGTACTCGAGGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGAGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTcct                                       <  2:1089663/150‑2 (MQ=255)

                                                                                                               aTCGACACCCCGGGCCACGTTGACTTCACCATCGAAGTCGAGCGTTCGCTCCGCGTTCTCGACGGTGCAATCGCTCTTCTCGATGCGAATGCTGGCGTAGAGCCACAGACCGAAACTGTTTGGCGTCAGGCTGAGAAGTACCACGTTcca                                       >  2:798661/1‑149 (MQ=255)

                                                                                                               aTCGACACCCCGGGCCACGTTGACTTCACCATCGAAGTCGAGCGTTCGCTCCGCGTTCTCGACGGTGCAATCGCTCTTCTCGATGCGAATGCTGGCGTAGAGCCACAGACCGAAACTGTTTGGCGTCAGGCTGAGAAGTACCACGTTcca                                       >  2:1593985/1‑149 (MQ=255)

                                                                                                                  gATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCgt                                    >  1:3949624/1‑150 (MQ=255)

                                                                                                                   acaCCCCGGGCCACGTTGACTTCACCATCGAAGTCGAGCGTTCGCTCCGCGTTCTCGACGGTGCAATCGCTCTTCTCGATGCGAATGCTGGCGTAGAGCCACAGACCGAAACTGTTTGGCGTCAGGCTGAGAAGTACCACGTTCCACgta                                   >  1:4783236/3‑149 (MQ=255)

                                                                                                                   acaCCCCGGGCCACGTTGACTTCACCATCGAAGTCGAGCGTTCGCTCCGCGTTCTCGACGGTGCAATCGCTCTTCTCGATGCGAATGCTGGCGTAGAGCCACAGACCGAAACTGTTTGGCGTCAGGCTGAGAAGTACCACGTTCCACgta                                   >  1:4783331/3‑149 (MQ=255)

                                                                                                                    tACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCACCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCgtgt                                  >  1:4322088/1‑150 (MQ=255)

                                                                                                                     aCCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGtgtt                                 >  2:3180063/1‑150 (MQ=255)

                                                                                                                       cccTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGtgttgt                               <  1:5073/150‑1 (MQ=255)

                                                                                                                       cccTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGtgttgt                               <  1:3599679/150‑1 (MQ=255)

                                                                                                                          tGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTa                            <  2:1969/150‑1 (MQ=255)

                                                                                                                             ccACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGt                         <  1:438066/150‑1 (MQ=255)

                                                                                                                             ccACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGt                         <  1:2377591/150‑1 (MQ=255)

                                                                                                                             ccACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGt                         <  2:3245328/150‑1 (MQ=255)

                                                                                                                              cACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTg                        >  2:1622076/1‑150 (MQ=255)

                                                                                                                                 gTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCa                                                             >  1:2484462/1‑110 (MQ=255)

                                                                                                                                 gTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCa                                                             <  2:2484462/110‑1 (MQ=255)

                                                                                                                                 gTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAAc                     >  1:1500138/1‑150 (MQ=255)

                                                                                                                                  ttGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACa                    >  1:79395/1‑150 (MQ=255)

                                                                                                                                   tGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACaa                   <  2:3856338/150‑1 (MQ=255)

                                                                                                                                    gACATCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAg                  <  2:2758907/150‑1 (MQ=255)

                                                                                                                                    gACATCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAg                  <  2:2758890/150‑1 (MQ=255)

                                                                                                                                     aCTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGa                 >  2:4237940/1‑150 (MQ=255)

                                                                                                                                       ttCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATg               >  2:3619158/1‑150 (MQ=255)

                                                                                                                                       ttCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATg               <  2:3305452/150‑1 (MQ=255)

                                                                                                                                       ttCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATg               >  1:1216578/1‑150 (MQ=255)

                                                                                                                                        tCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGtgtt                                 >  2:1974179/1‑131 (MQ=255)

                                                                                                                                        tCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGtgtt                                 <  1:1974179/131‑1 (MQ=255)

                                                                                                                                           ccATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCCTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATGGAcc           >  1:1004054/1‑150 (MQ=255)

                                                                                                                                             aTTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATGGACCGt         >  2:2402890/1‑150 (MQ=255)

                                                                                                                                              ttGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATGGACCGTg        >  2:4649110/1‑150 (MQ=255)

                                                                                                                                                   gTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATGGACCGTGCTGGt   <  1:3075051/150‑1 (MQ=255)

                                                                                                                                                    tAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATGGACCGTGCTGGTg  >  1:4968200/1‑150 (MQ=255)

                                                                                                                                                    tAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATGGACCGTGCTGGTg  >  1:4968196/1‑150 (MQ=255)

ACCGACTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGGAAAGGTTCCCGTGGTCAGTATGACAACTACCGCGTAAACGTTATCGATACCCCTGGCCACGTTGACTTCACCATTGAAGTAGAGCGTTCGCTGCGTGTACTCGACGGCGCGGTCGTTGTGTTCTGTGGCACCTCCGGCGTTGAGCCGCAGTCCGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTTCCGCGTGTTGTTTACGTGAACAAGATGGACCGTGCTGGTG  >  NC_002947/547301‑547598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`